Serum lipids and prostate cancer

Background: Conflicting results are found in the literature relating serum lipids levels and prostate cancer. Some results imply a relationship between them; others contradict this association. The purpose of this study was to investigate a possible association between serum lipids levels and prostate cancer, at time of diagnosis. Methods: We measured serum levels of total cholesterol, HDL cholesterol, LDL cholesterol, and triglycerides in 237 patients submitted to a prostate biopsy, with PSA between 2 and 10 ng/ml. Patients without cancer at biopsy were used as controls, and the others were considered as cases. No information about lipid-lowering therapy, including statins, was available neither in cases nor in controls. Cases were divided into risk groups, according to the disease severity, based on staging. Lipids levels were compared between groups, using parametric and nonparametric tests. Logistic regression analysis and odds ratios were calculated. Results: LDL and total cholesterol levels were lower in patients with cancer, with the difference being statistically significant for LDL cholesterol (p = 0.010) and borderline for total cholesterol (p = 0.050). No significant differences were found between the several risk groups. Odds ratios for low LDL cholesterol (<130 mg/dl) and low total cholesterol (<200 mg/dl), with prostate cancer as the outcome, were 1.983 and 1.703, respectively. There were no significant differences between cases and controls for the other lipids. Conclusion: Lower LDL cholesterol (<130 mg/dl) and lower total cholesterol (<200 mg/dl) serum levels seem to associate with prostate cancer, at time of diagnosis.publishersversionpublishe

Structural analysis of the maltese cross unit in the Tapajós National Forest, State of Pará, Brazil

O presente trabalho estudou a forma estrutural do conglomerado cruz de malta, considerando três variáveis para quantificar volume de madeira. Foram usados 25 diferentes tamanhos de subparcelas, definindo-se a sua magnitude ideal através do Método da Curvatura Máxima e, a partir dela, estudando-se 17 diferentes distâncias das subparcelas ao ponto central do conglomerado. Os resultados recomendaram um tamanho igual a 0,32 ha para uma distância de 100 m. Quando utilizado um tamanho de subparcela igual a 0,25 ha, a distância delas ao ponto central não deve ser inferior a 50 m. Observou-se que o número de subparcelas necessárias para estruturar um conglomerado aumenta com a diminuição do tamanho delas, o que foi verificado quando a magnitude atingiu 0,12 ha, com os valores dos coeficientes de correlação intraconglomerado para as três variáveis estudadas variando entre 0,055 e 0,087 e, consequentemente, o número de subparcelas variando de 5,596 a 7,193, denotando ser mais eficiente uma formatação conglomerada cruzada com oito subparcelas.Palavras-chave:  Inventário Florestal; amostragem por conglomerado; tamanho de parcela; correlação intraconglomerado; estrutura amostral. AbstractStructural analysis of the maltese cross unit in the Tapajós National Forest, State of Pará, Brazil. This work studied the structural form of the Maltese cross cluster, considering three variables to quantify wood volume, using 25 different sizes of sub-sampling, to define the cluster ideal size through the Maximum Curvature Method and, from this, to study 17 different distances from the sub-sampling to the cluster central point. The results indicated a size equal to 0,32 ha for a distance of 100 m. When used a sub-sampling size of 0,25 ha, the distance between them and the central point can not be less than 50 m. It was observed that the number of sub-sampling necessary to structuralize a cluster increases when the size decreases, which was verified when the magnitude reached 0,12 ha, where the values of the intracluster correlation coefficients for the three studied variables varied between 0,055 to 0,087 and, consequently, the number of sub-sampling varied from 5,596 to 7,193, denoting to be more efficient a cluster structure in crossed shape, but showing eight sub-sampling.Keywords:              Forest Inventory; cluster sampling; sub-sampling size; intracluster correlation; sampling structure.This work studied the structural form of the Maltese cross cluster, considering three variables to quantify wood volume, using 25 different sizes of sub-sampling, to define the cluster ideal size through the Maximum Curvature Method and, from this, to study 17 different distances from the sub-sampling to the cluster central point. The results indicated a size equal to 0,32 ha for a distance of 100 m. When used a sub-sampling size of 0,25 ha, the distance between them and the central point can not be less than 50 m. It was observed that the number of sub-sampling necessary to structuralize a cluster increases when the size decreases, which was verified when the magnitude reached 0,12 ha, where the values of the intracluster correlation coefficients for the three studied variables varied between 0,055 to 0,087 and, consequently, the number of sub-sampling varied from 5,596 to 7,193, denoting to be more efficient a cluster structure in crossed shape, but showing eight sub-sampling

Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)

Although concentrations of pharmaceutical compounds in aquatic ecosystems are low, they can cause toxic effects on organisms. The aim of this study was to evaluate the effects of diclofenac (DCF), a non-steroidal anti-inflammatory drug, and caffeine (CAF), a central nervous system stimulant, both alone or combined, in Astyanax altiparanae males under acute exposure (96 h), measuring neurotoxicity biomarkers, antioxidant response and damage at biochemical and cellular levels. DCF concentration in water, separated and combined, was 3.08 mg L−1 and that of CAF was 9.59 mg L−1. To assess neurotoxicity, brain and muscle acetylcholinesterase (AChE) activities were measured. To evaluate oxidative stress, the enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione S-transferase (GST), as well as lipoperoxidation (LPO), were analyzed in liver and gills. Activity of hepatic cyclooxygenase (COX) was also evaluated. Genotoxicity was assessed in blood using comet assay and micronucleus test, as well as nuclear abnormalities. DCF and CAF, alone or combined, had neither effect on AChE activity, nor in the activity of SOD, CAT, GPx and GST in gills. In liver, DCF inhibited SOD and GPx activity, CAF inhibited CAT activity, the mixture inhibited SOD and GST activity; although only fish exposed to CAF showed increased hepatic LPO. Under these experimental conditions, no effect on COX activity was observed, nor cytotoxic and genotoxic damage. The most pronounced effects were caused by the drugs separately, since both compounds altered the enzymes, but only CAF triggered LPO, showing more harmful effects.Fil: Muñoz Peñuela, Marcela. Universidade de Sao Paulo; BrasilFil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Dal'Olio Gomes, Aline. Universidade de Sao Paulo; BrasilFil: Tolussi, Carlos Eduardo. Universidade Anhembi Morumbi; BrasilFil: Branco, Giovana Souza. Universidade de Sao Paulo; BrasilFil: Pinheiro, João Paulo Silva. Universidade de Sao Paulo; BrasilFil: Godoi, Filipe Guilherme Andrade de. Universidade de Sao Paulo; BrasilFil: Moreira, Renata Guimarães. Universidade de Sao Paulo; Brasi

Absence of Tau triggers age-dependent sciatic nerve morphofunctional deficits and motor impairment

Dementia is the cardinal feature of Alzheimer's disease (AD), yet the clinical symptoms of this disorder also include a marked loss of motor function. Tau abnormal hyperphosphorylation and malfunction are well-established key events in AD neuropathology but the impact of the loss of normal Tau function in neuronal degeneration and subsequent behavioral deficits is still debated. While Tau reduction has been increasingly suggested as therapeutic strategy against neurodegeneration, particularly in AD, there is controversial evidence about whether loss of Tau progressively impacts on motor function arguing about damage of CNS motor components. Using a variety of motor-related tests, we herein provide evidence of an age-dependent motor impairment in Tau-/- animals that is accompanied by ultrastructural and functional impairments of the efferent fibers that convey motor-related information. Specifically, we show that the sciatic nerve of old (17-22-months) Tau-/- mice displays increased degenerating myelinated fibers and diminished conduction properties, as compared to age-matched wild-type (Tau+/+) littermates and younger (4-6months) Tau-/- and Tau+/+ mice. In addition, the sciatic nerves of Tau-/- mice exhibit a progressive hypomyelination (assessed by g-ratio) specifically affecting large-diameter, motor-related axons in old animals. These findings suggest that loss of Tau protein may progressively impact on peripheral motor system.The work was supported by grants 'PTDC/SAU-NMC/113934/2009,' 'PTDC/SAU-NSC/118194/2010,' 'SFRH/BPD/97281/2013,' PTDC/SAU-NSC/118194/2010,' 'SFRH/BPD/80118/2011,' 'SFRH/BD/89714/2012' funded by FCT-Portuguese Foundation for Science and Technology and project DoIT-Desenvolvimento e Operacionalizacao da Investigacao de Translacao (No do projeto 13853), funded by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout the Programa Operacional Fatores de Competitividade (POFC). In addition, this work was also co-financed by European Union FP7 project SwitchBox (NS) and the Portuguese North Regional Operational Program (ON.2 - O Novo Norte) under the National Strategic Reference Framework (QREN), through the European Regional Development Fund (FEDER).info:eu-repo/semantics/publishedVersio

Cefaleias: Diagnóstico Diferencial e Abordagens Terapêuticas: Um estudo das diferentes tipos de cefaleias, incluindo enxaqueca e cefaleia tensional, e suas opções de tratamento.

Headaches, including migraine and tension-type headache, represent one of the most common medical complaints worldwide, significantly impacting the quality of life of patients. This review article examines therapeutic approaches to these conditions, emphasizing the importance of differential diagnosis and individualized treatment. The findings from the reviewed studies reveal that individualization of treatment is crucial as each patient presents a unique combination of symptoms, triggers, and treatment response. This necessitates a personalized approach that takes individual characteristics into account. Furthermore, non-pharmacological therapies such as cognitive-behavioral therapy and acupuncture have shown efficacy in headache treatment. These therapies provide valuable alternatives to pharmacological interventions, highlighting the importance of a multidisciplinary approach. Identifying and eliminating triggers is another crucial aspect of headache management. Patients with migraines can benefit from identifying specific triggers, while reducing chronic stress plays a fundamental role in tension-type headache prevention. However, the reviewed studies also point out limitations, including the lack of longer follow-up periods and individual variability in treatment response. In summary, the treatment of headaches is a complex area that requires a holistic and personalized approach. Ongoing research is essential to enhance the understanding of these conditions and the effectiveness of therapeutic approaches, aiming to improve the quality of life of patients.As cefaleias, incluindo a enxaqueca e a cefaleia tensional, representam uma das queixas médicas mais comuns em todo o mundo, impactando significativamente a qualidade de vida dos pacientes. Este artigo de revisão examina as abordagens terapêuticas dessas condições, destacando a importância do diagnóstico diferencial e da individualização do tratamento. Os resultados dos estudos analisados revelam que a individualização do tratamento é fundamental, uma vez que cada paciente apresenta uma combinação única de sintomas, fatores desencadeantes e resposta ao tratamento. Isso requer uma abordagem personalizada que leve em consideração as características individuais. Além disso, as terapias não farmacológicas, como a terapia cognitivo-comportamental e a acupuntura, demonstraram eficácia no tratamento das cefaleias. Estas terapias oferecem alternativas valiosas às intervenções farmacológicas, destacando a importância de uma abordagem multidisciplinar. Identificar e eliminar fatores desencadeantes é outro aspecto crucial da gestão das cefaleias. Pacientes com enxaqueca podem se beneficiar ao identificar gatilhos específicos, enquanto a redução do estresse crônico desempenha um papel fundamental na prevenção da cefaleia tensional. No entanto, os estudos revisados também apontam limitações, incluindo a falta de períodos de acompanhamento mais longos e a variabilidade individual na resposta ao tratamento. Em resumo, o tratamento das cefaleias é uma área complexa que exige uma abordagem holística e personalizada. A pesquisa contínua é essencial para aprimorar o entendimento dessas condições e a eficácia das abordagens terapêuticas, visando melhorar a qualidade de vida dos pacientes

CH4 and N2O fluxes from planted forests and native Cerrado ecosystems in Brazil

Forest soils are N2O sources and commonly act as CH4 sinks. This study evaluated the dynamics of the CH4 and N2O fluxes of soils under Eucalyptus plantations and native Cerrado vegetation, as well as possible interactions between environmental factors and fluxes. The study was carried out in the Distrito Federal, Brazil, during 26 months, in three areas: in two stands of the hybrid Eucalyptus urophylla × Eucalyptus grandis, planted in 2011 (E1), and in 2009 (E2) and native Cerrado vegetation (CE). Measurements to determine the fluxes in a closed static chamber were carried out from Oct 2013 to Nov 2015. Soil and climate factors were monitored. During the study period, the mean CH4 fluxes were –22.48, –8.38 and –1.31 μg CH4 m–2 h–1 and the mean N2O fluxes 5.45, 4.85 and 3.85 μg N2O m–2 h–1 from E1, E2 and CE, respectively. Seasonality affected plantations in the studied sites. Cumulative CH4 influxes were calculated (year-1: –1.86 to -0.63 kg ha–1 yr–1; year-2: –1.85 to –1.34 kg ha–1 yr–1). Cumulative N2O fluxes in the three sites were ≤ 0.85 kg ha–1 yr–1. The change in land use from Cerrado to Eucalyptus plantations did not significantly changed regarding greenhouse gases (GHG), compared to the native vegetation. Flux rates of both gases (N2O and CH4) were low. Temporal variations in GHG fluxes and different ages of the stands did not cause significant differences in cumulative annual fluxes

Characterization and selection of torch ginger for cut flower

Torch ginger, Etlingera elatior, is among the most cultivated tropical flower of its genus. Its great acceptability in the market led researchers to improve production characteristics and to adapt it for commercialization. This study aimed to characterize 10 genotypes of torch ginger in order to select and recommend them as cut flower. The experiment ran from Jan 2014 to Dec 2016 in Paulista, Brazil and nine agro-morphological characteristics were evaluated to build a grading system that determined their suitability for cut flower. The IAC 26 and IAC 34 genotypes were classified as poorly suitable, for they presented fresh mass exceeding 237 g. They also presented less than 19 flower stems per clump and only one month with ≥1 floral stem produced in 2015. The genotypes IAC 1, IAC 2, IAC 3, IAC 11, IAC 13 and the cultivar IAC Prumirim were classified as suitable, although they obtained intermediate scores for most of the evaluated criteria. The genotype IAC 41 and the cultivar IAC Camburi were classified as very suitable for cut flower, beginning of flowering period at 12 months, produce 33.25 and 41.20 flower stem per clump, respectively, and present seven months with production of more than one floral stem per clump in 2016, achieving maximum scores from most of the evaluated characteristics

Biomass and nutrients in three species of Parkia plantings on degraded area in Central Amazon

AbstractBiomass and nutrients partitioning strategies in tree species may reflect their ability to survive in plantations on degraded areas. The objective of this study was to investigate the content of biomass and nutrients in tree components of Parkia multijuga, Parkia nitida and Parkia pendula on plantings in degraded area in Manaus, AM. The biomass was determined by the harvest method in six trees of each species, which were subdivided into leaves, fine branches (Ø Ca > K > Mg > P. Parkia multijuga, by adopting better strategies of distribution of biomass and nutrients, it is a recommended species for reforestation programs on degraded sites in the Amazon.Keywords: Native forest species; forest nutrition; reforestation; restoration.As estratégias de distribuição de biomassa e nutrientes utilizadas pelas espécies florestais podem refletir sua capacidade de sobrevivência em plantios sobre áreas degradadas. O objetivo deste estudo foi quantificar os estoques de biomassa e nutrientes nos compartimentos arbóreos de Parkia multijuga, Parkia nitida e Parkia pendula em plantios sobre área degradada em Manaus, AM. A biomassa foi determinada pelo método destrutivo em seis árvores de cada espécie, que foram compartimentadas em: folhas, galhos finos (Ø Ca > K > Mg > P. O fato de Parkia multijuga adotar estratégias de alocação de biomassa e nutrientes que favorecerão seu desempenho sobre sítios com baixa disponibilidade de recursos sustenta sua indicação para a composição de programas de reflorestamento em áreas degradadas na Amazônia.Palavras-chave: Espécies florestais nativas; nutrição florestal; reflorestamento; restauração. AbstractBiomass and nutrients in three species of Parkia plantings on degraded area in Central Amazon. Biomass and nutrients partitioning strategies in tree species may reflect their ability to survive in plantations on degraded areas. The objective of this study was to investigate the content of biomass and nutrients in tree components of Parkia multijuga, Parkia nitida and Parkia pendula on plantings in degraded area in Manaus, AM. The biomass was determined by the harvest method in six trees of each species, which were subdivided into leaves, fine branches (Ø Ca > K > Mg > P. Parkia multijuga, by adopting better strategies of distribution of biomass and nutrients, it is a recommended species for reforestation programs on degraded sites in the Amazon.Keywords: Native forest species; forest nutrition; reforestation; restoration

Association of 3-Phenoxybenzoic Acid Exposure during Pregnancy with Maternal Outcomes and Newborn Anthropometric Measures: Results from the IoMum Cohort Study

The aims of this study were to characterize the exposure of pregnant women living in Portugal to 3-phenoxybenzoic acid (3-PBA) and to evaluate the association of this exposure with maternal outcomes and newborn anthropometric measures. We also aimed to compare exposure in summer with exposure in winter. Pregnant women attending ultrasound scans from April 2018 to April 2019 at a central hospital in Porto, Portugal, were invited to participate. Inclusion criteria were: gestational week between 10 and 13, confirmed fetal vitality, and a signature of informed consent. 3-PBA was measured in spot urine samples by gas chromatography with mass spectrometry (GC-MS). The median 3-PBA concentration was 0.263 (0.167; 0.458) μg/g creatinine (n = 145). 3-PBA excretion was negatively associated with maternal pre-pregnancy body mass index (BMI) (p = 0.049), and it was higher during the summer when compared to winter (p < 0.001). The frequency of fish or yogurt consumption was associated positively with 3-PBA excretion, particularly during the winter (p = 0.002 and p = 0.015, respectively), when environmental exposure is low. Moreover, 3-PBA was associated with levothyroxine use (p = 0.01), a proxy for hypothyroidism, which could be due to a putative 3-PBA—thyroid hormone antagonistic effect. 3-PBA levels were not associated with the anthropometric measures of the newborn. In conclusion, pregnant women living in Portugal are exposed to 3-PBA, particularly during summer, and this exposure may be associated with maternal clinical features.This article was supported by national funds through the FCT Foundation for Science and Technology, I.P., within the scope of the projects RISE - LA/P/0053/2020; CINTESIS, R&D UNIT (reference UIDB/4255/2020) and LAQV (references: UIDB/50006/2020 AND UIDP/50006/2020). Virgínia Cruz Fernandes was funded by FCT/MCTES (Foundation for Science and Technology and Ministry of Science, Technology and Higher Education) and the ESF (European Social Fund) through NORTE 2020 (North Region Operational Program) through a grant of Post-Doc (reference SFRH/BPD/109153/2015). Juliana Guimarães was funded by FCT/MCTES (Foundation for Science and Technology and Ministry of Science, Technology and Higher Education) under CINTESIS by a PhD scholarship (reference UI/BD/152087/2021). The authors would like to thank the kind participation of all pregnant women and the support of the recruitment activities by the health professionals of the Department of Obstetrics, Centro Hospitalar Universitário S. João, Porto, Portugal and also to the professionals of REQUIMTE/LAQV, Instituto Superior de Engenharia, Politécnico do Porto, for their collaboration in carrying out the analyzes of the 3-PBA metabolite. This work received support from PT national funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through the projects LA/P/0053/2020; UIDB/4255/2020; UIDB/5006/2020 and UIDP/50006/2020. Virgínia Cruz Fernandes thanks FCT/MCTES (Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) and ESF (European Social Fund) through NORTE 2020 (Programa Operacional Região Norte) for his/her Post-Doc grant ref. SFRH/BPD/109153/2015). Juliana Guimarães was funded by FCT/MCTES (Foundation for Science and Technology and Ministry of Science, Technology and Higher Education) under CINTESIS by a PhD scholarship (reference UI/BD/152087/2021).info:eu-repo/semantics/publishedVersio