Citología cutánea veterinaria

La citología es una herramienta cada vez más utilizada y reconocida en el diagnóstico veterinario, y ha conseguido un sólido asentamiento en muchos países donde se utiliza de forma rutinaria en clínica. Es así porque aporta información que el veterinario demanda de forma rápida, muy deseable a la hora de instaurar tratamiento, sencilla, barata, indolora y muy versátil, ya que todas las células del organismo pueden someterse a estudio. Es por ello básicamente práctica y eficaz. Debido a la cantidad de lesiones que aparecen en la piel y tejido subcutáneo la citología en estas patologías para realizar un primer acercamiento diagnóstico, que puede ser definitivo en ocasiones, y reducir la lista de diagnósticos diferenciales posibles e incluso ahorrar en tiempo, coste y la realización de otras pruebas adicionales. Este artículo va dirigido al estudio de la citología cutánea, considerando la correcta obtención de muestras, la población celular que podemos encontrar y los procesos más frecuentemente implicados

Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes

<p>Abstract</p> <p>Background</p> <p>The recently described Type VI Secretion System (T6SS) represents a new paradigm of protein secretion in bacteria. A number of bioinformatic studies have been conducted to identify T6SS gene clusters in the available bacterial genome sequences. According to these studies, <it>Salmonella </it>harbors a unique T6SS encoded in the <it>Salmonella </it>Pathogenicity Island 6 (SPI-6). Since these studies only considered few <it>Salmonella </it>genomes, the present work aimed to identify novel T6SS loci by <it>in silico </it>analysis of every genome sequence of <it>Salmonella </it>available.</p> <p>Results</p> <p>The analysis of sequencing data from 44 completed or in progress <it>Salmonella </it>genome projects allowed the identification of 3 novel T6SS loci. These clusters are located in differentially-distributed genomic islands we designated SPI-19, SPI-20 and SPI-21, respectively. SPI-19 was identified in a subset of <it>S. enterica </it>serotypes including Dublin, Weltevreden, Agona, Gallinarum and Enteritidis. In the later, an internal deletion eliminated most of the island. On the other hand, SPI-20 and SPI-21 were restricted to <it>S. enterica </it>subspecies <it>arizonae </it>(IIIa) serotype 62:z4,z23:-. Remarkably, SPI-21 encodes a VgrG protein containing a C-terminal extension similar to S-type pyocins of <it>Pseudomonas aeruginosa</it>. This is not only the first evolved VgrG described in <it>Salmonella</it>, but also the first evolved VgrG including a pyocin domain described so far in the literature. In addition, the data indicate that SPI-6 T6SS is widely distributed in <it>S. enterica </it>and absent in serotypes Enteritidis, Gallinarum, Agona, Javiana, Paratyphi B, Virchow, IIIa 62:z4,z23:- and IIIb 61:1,v:1,5,(7). Interestingly, while some serotypes harbor multiple T6SS (Dublin, Weltvreden and IIIa 62:z4,z23:-) others do not encode for any (Enteritidis, Paratyphi B, Javiana, Virchow and IIIb 61:1,v:1,5,(7)). Comparative and phylogenetic analyses indicate that the 4 T6SS loci in <it>Salmonella </it>have a distinct evolutionary history. Finally, we identified an orphan Hcp-like protein containing the Hcp/COG3157 domain linked to a C-terminal extension. We propose to designate this and related proteins as "evolved Hcps".</p> <p>Conclusion</p> <p>Altogether, our data suggest that (i) the <it>Salmonella </it>T6SS loci were acquired by independent lateral transfer events and (ii) evolved to contribute in the adaptation of the serotypes to different lifestyles and environments, including animal hosts. Notably, the presence of an evolved VgrG protein related to pyocins suggests a novel role for T6SS in bacterial killing. Future studies on the roles of the identified T6SS loci will expand our knowledge on <it>Salmonella </it>pathogenesis and host specificity.</p

Chapter Metabolomics for soil contamination assessment

Regional geograph

Study of cross-spectra of velocity components and temperature series in a nocturnal boundary layer

Póster presentado en: European Geosciences Union General Assembly celebrada del 2 al 7 de mayo de 2010 en Viena.This research has been funded by the Spanish Ministry of Science and Innovation (projects CGL 2006-12474-C03-03 and CGL2009-12797-C03-03). GR58/08 program (supported by BSCH and UCM) has also partially financed this work through the Research Group “Micrometeorology and Climate Variability” (nº 910437)

The second flight of the Sunrise Balloon-borne Solar Observatory: overview of instrument updates, the flight, the data, and first results

S. K. Solanki et. al.©2017 The American Astronomical Society. All rights reserved.The Sunrise balloon-borne solar observatory, consisting of a 1 m aperture telescope that provides a stabilized image to a UV filter imager and an imaging vector polarimeter, carried out its second science flight in 2013 June. It provided observations of parts of active regions at high spatial resolution, including the first high-resolution images in the Mg ii k line. The obtained data are of very high quality, with the best UV images reaching the diffraction limit of the telescope at 3000 Å after Multi-Frame Blind Deconvolution reconstruction accounting for phase-diversity information. Here a brief update is given of the instruments and the data reduction techniques, which includes an inversion of the polarimetric data. Mainly those aspects that evolved compared with the first flight are described. A tabular overview of the observations is given. In addition, an example time series of a part of the emerging active region NOAA AR 11768 observed relatively close to disk center is described and discussed in some detail. The observations cover the pores in the trailing polarity of the active region, as well as the polarity inversion line where flux emergence was ongoing and a small flare-like brightening occurred in the course of the time series. The pores are found to contain magnetic field strengths ranging up to 2500 G, and while large pores are clearly darker and cooler than the quiet Sun in all layers of the photosphere, the temperature and brightness of small pores approach or even exceed those of the quiet Sun in the upper photosphere.The German contribution to Sunrise and its reflight was funded by the Max Planck Foundation, the Strategic Innovations Fund of the President of the Max Planck Society (MPG), DLR, and private donations by supporting members of the Max Planck Society, which is gratefully acknowledged. The Spanish contribution was funded by the Ministerio de Economía y Competitividad under Projects ESP2013-47349-C6 and ESP2014-56169-C6, partially using European FEDER funds. The National Center for Atmospheric Research is sponsored by the National Science Foundation. The HAO contribution was partly funded through NASA grant number NNX13AE95G. This work was partly supported by the BK21 plus program through the National Research Foundation (NRF) funded by the Ministry of Education of Korea. L.G. acknowledges research funding from the State of Lower Saxony, Germany. SDO is a mission of NASA's Living With a Star (LWS) program. The SDO/HMI data were provided by the Joint Science Operation Center (JSOC). The National Solar Observatory (NSO) is operated by the Association of Universities for Research in Astronomy (AURA) Inc. under a cooperative agreement with the National Science Foundation.Peer reviewe

E-Commerce, social media and Social Inclusion: A Typology of Users Over 60 Years of Age in Spain

The growth of e-commerce and the ageing of the world population converges in a situation which represents significant challenges for the economy: a large population of over 65year-olds with major limitations for online shopping. The digital division, although reduced in certain sectors, in others, such as e-commerce persists. This research involves the study of 405 subjects, Spanish seniors aged between 60 and 79, to discover and analyse the barriers, motivators and behaviour with regards to shopping online. Using a telephone survey and a random sample, a typology of users was established as per their behaviour and perception of e-commerce. The main finding is the segmentation in eleven typologies of users, identified through cluster analysis. The major conclusions confirm the barriers related to data security or levels of confidence. Furthermore, with basic digital skills, seniors consider themselves capable of completing purchases on the Internet. Online shoppers feel empowered being digitally self-sufficient, while non-shoppers desiring to be independent, require the implementation of measures to aid the process on commercial websites.El crecimiento del comercio electrónico y el envejecimiento de la población mundial convergen en una situación que representa importantes retos para la economía: una gran población de mayores de 65 años con grandes limitaciones para realizar compras en línea. La brecha digital, aunque se ha reducido en algunos sectores, en otros, como el del comercio electrónico, persiste. Esta investigación consiste en un estudio de 405 sujetos, españoles de entre 60 y 79 años, para conocer y analizar las barreras, las motivaciones y el comportamiento ante las compras online. Mediante una encuesta telefónica y con una muestra aleatoria, se estableció una tipología de usuarios según su comportamiento y percepción del comercio electrónico. El principal hallazgo es la segmentación en once tipologías de usuarios, identificadas a través del análisis de clúster. Las principales conclusiones confirman las barreras relacionadas con la seguridad de los datos o los niveles de confianza. Por otro lado, con unas habilidades digitales básicas, los mayores se consideran capaces de realizar compras en Internet. Los compradores online se sienten capacitados siendo autosuficientes digitalmente, mientras que los no compradores que desean ser independientes, requieren la implementación de medidas de ayuda al proceso en los sitios web comerciales

Veinticinco años (1986-2011) de monitorización de varamientos de mamíferos marinos en el litoral de Doñana (Huelva, SO España)

Peer reviewe