82 research outputs found
TB191: Conservation and Management of Native Bees in Cranberry
Threats to agriculturally important pollinators have serious implications for human beings. A loss of bees translates to less successful crop pollination, thus reduced yield and poorer quality fruits. Native bees have the potential to serve as commercial pollinators. A diverse pollinator complex comprised of both honey bees and native bees should result in stable pollination levels and should be resistant to threats such as disease, fluctuating honey and crop prices, and honey bee transportation costs. Adding the goal of native bee conservation to land management increases the ecological integrity of an ecosystem by conserving a unique biological interaction that is the basis for most native wild plant reproduction. This report describes pollination in the cranberry agroecosystem and outlines steps to take to manage native bees in cranberry.https://digitalcommons.library.umaine.edu/aes_techbulletin/1017/thumbnail.jp
Planar Cell Polarity Enables Posterior Localization of Nodal Cilia and Left-Right Axis Determination during Mouse and Xenopus Embryogenesis
Left-right asymmetry in vertebrates is initiated in an early embryonic structure called the ventral node in human and mouse, and the gastrocoel roof plate (GRP) in the frog. Within these structures, each epithelial cell bears a single motile cilium, and the concerted beating of these cilia produces a leftward fluid flow that is required to initiate left-right asymmetric gene expression. The leftward fluid flow is thought to result from the posterior tilt of the cilia, which protrude from near the posterior portion of each cell's apical surface. The cells, therefore, display a morphological planar polarization. Planar cell polarity (PCP) is manifested as the coordinated, polarized orientation of cells within epithelial sheets, or as directional cell migration and intercalation during convergent extension. A set of evolutionarily conserved proteins regulates PCP. Here, we provide evidence that vertebrate PCP proteins regulate planar polarity in the mouse ventral node and in the Xenopus gastrocoel roof plate. Asymmetric anterior localization of VANGL1 and PRICKLE2 (PK2) in mouse ventral node cells indicates that these cells are planar polarized by a conserved molecular mechanism. A weakly penetrant Vangl1 mutant phenotype suggests that compromised Vangl1 function may be associated with left-right laterality defects. Stronger functional evidence comes from the Xenopus GRP, where we show that perturbation of VANGL2 protein function disrupts the posterior localization of motile cilia that is required for leftward fluid flow, and causes aberrant expression of the left side-specific gene Nodal. The observation of anterior-posterior PCP in the mouse and in Xenopus embryonic organizers reflects a strong evolutionary conservation of this mechanism that is important for body plan determination
Reductions in entrée energy density increase children\u27s vegetable intake and reduce energy intake
The energy density (ED; kcal/g) of an entrée influences children\u27s energy intake (EI), but the effect of simultaneously changing both ED and portion size of an entrée on preschool children\u27s EI is unknown. In this within-subject crossover study, 3- to 5-year-old children (30 boys, 31 girls) in a daycare facility were served a test lunch once/week for 4 weeks. The amount and type of vegetables and cheeses incorporated into the sauce of a pasta entrée were manipulated to create two versions that varied in ED by 25% (1.6 or 1.2 kcal/g). Across the weeks, each version of the entrée was served to the children in each of two portion sizes (400 or 300 g). Lunch, consumed ad libitum, also included carrots, applesauce, and milk. Decreasing ED of the entrée by 25% significantly (P < 0.0001) reduced children\u27s EI of the entrée by 25% (63.1 8.3 kcal) and EI at lunch by 17% (60.7 8.9 kcal). Increasing the proportion of vegetables in the pasta entrée increased children\u27s vegetable intake at lunch by half of a serving of vegetables (P < 0.01). Decreasing portion size of the entrée by 25% did not significantly affect children\u27s total food intake or EI at lunch. Therefore, reducing the ED of a lunch entrée resulted in a reduction in children\u27s EI from the entrée and from the meal in both portion size conditions. Decreasing ED by incorporating more vegetables into recipes is an effective way of reducing children\u27s EI while increasing their vegetable intake.<br /
Evaluation of an Intervention Providing HPV Vaccine in Schools
To conduct outcome and process evaluations of school-located HPV vaccination clinics in partnership with a local health department
LOOC UP: Locating and observing optical counterparts to gravitational wave bursts
Gravitational wave (GW) bursts (short duration signals) are expected to be
associated with highly energetic astrophysical processes. With such high
energies present, it is likely these astrophysical events will have signatures
in the EM spectrum as well as in gravitational radiation. We have initiated a
program, "Locating and Observing Optical Counterparts to Unmodeled Pulses in
Gravitational Waves" (LOOC UP) to promptly search for counterparts to GW burst
candidates. The proposed method analyzes near real-time data from the
LIGO-Virgo network, and then uses a telescope network to seek optical-transient
counterparts to candidate GW signals. We carried out a pilot study using
S5/VSR1 data from the LIGO-Virgo network to develop methods and software tools
for such a search. We will present the method, with an emphasis on the
potential for such a search to be carried out during the next science run of
LIGO and Virgo, expected to begin in 2009.Comment: 11 pages, 2 figures; v2) added acknowledgments, additional
references, and minor text changes v3) added 1 figure, additional references,
and minor text changes. v4) Updated references and acknowledgments. To be
published in the GWDAW 12 Conf. Proc. by Classical and Quantum Gravit
Galactic Globular and Open Clusters in the Sloan Digital Sky Survey. I. Crowded Field Photometry and Cluster Fiducial Sequences in ugriz
We present photometry for globular and open cluster stars observed with the
Sloan Digital Sky Survey (SDSS). In order to exploit over 100 million stellar
objects with r < 22.5 mag observed by SDSS, we need to understand the
characteristics of stars in the SDSS ugriz filters. While star clusters provide
important calibration samples for stellar colors, the regions close to globular
clusters, where the fraction of field stars is smallest, are too crowded for
the standard SDSS photometric pipeline to process. To complement the SDSS
imaging survey, we reduce the SDSS imaging data for crowded cluster fields
using the DAOPHOT/ALLFRAME suite of programs and present photometry for 17
globular clusters and 3 open clusters in a SDSS value-added catalog. Our
photometry and cluster fiducial sequences are on the native SDSS 2.5-meter
ugriz photometric system, and the fiducial sequences can be directly applied to
the SDSS photometry without relying upon any transformations. Model photometry
for red giant branch and main-sequence stars obtained by Girardi et al. cannot
be matched simultaneously to fiducial sequences; their colors differ by
~0.02-0.05 mag. Good agreement (< ~0.02 mag in colors) is found with Clem et
al. empirical fiducial sequences in u'g'r'i'z' when using the transformation
equations in Tucker et al.Comment: 30 pages, 25 figures. Accepted for publication in ApJS. Version with
high resolution figures available at
http://www.astronomy.ohio-state.edu/~deokkeun/AnJohnson.pd
Violence against primary school children with disabilities in Uganda: a cross-sectional study.
BACKGROUND: 150 million children live with disabilities globally, and a recent systematic review found 3 to 4 times the levels of violence versus non-disabled children in high income countries. However, almost nothing is known about violence against disabled children in lower income countries. We aim to explore the prevalence, patterns and risk factors for physical, sexual and emotional violence among disabled children attending primary school in Luwero District, Uganda. METHODS: We performed a secondary analysis of data from the baseline survey of the Good Schools Study. 3706 children and young adolescents aged 11-14 were randomly sampled from 42 primary schools. Descriptive statistics were computed and logistic regression models fitted. RESULTS: 8.8% of boys and 7.6% of girls reported a disability. Levels of violence against both disabled and non-disabled children were extremely high. Disabled girls report slightly more physical (99.1% vs 94.6%, p = 0.010) and considerably more sexual violence (23.6% vs 12.3%, p = 0.002) than non-disabled girls; for disabled and non-disabled boys, levels are not statistically different. The school environment is one of the main venues at which violence is occurring, but patterns differ by sex. Risk factors for violence are similar between disabled and non-disabled students. CONCLUSIONS: In Uganda, disabled girls are at particular risk of violence, notably sexual violence. Schools may be a promising venue for intervention delivery. Further research on the epidemiology and prevention of violence against disabled and non-disabled children in low income countries is urgently needed
LSST Science Book, Version 2.0
A survey that can cover the sky in optical bands over wide fields to faint
magnitudes with a fast cadence will enable many of the exciting science
opportunities of the next decade. The Large Synoptic Survey Telescope (LSST)
will have an effective aperture of 6.7 meters and an imaging camera with field
of view of 9.6 deg^2, and will be devoted to a ten-year imaging survey over
20,000 deg^2 south of +15 deg. Each pointing will be imaged 2000 times with
fifteen second exposures in six broad bands from 0.35 to 1.1 microns, to a
total point-source depth of r~27.5. The LSST Science Book describes the basic
parameters of the LSST hardware, software, and observing plans. The book
discusses educational and outreach opportunities, then goes on to describe a
broad range of science that LSST will revolutionize: mapping the inner and
outer Solar System, stellar populations in the Milky Way and nearby galaxies,
the structure of the Milky Way disk and halo and other objects in the Local
Volume, transient and variable objects both at low and high redshift, and the
properties of normal and active galaxies at low and high redshift. It then
turns to far-field cosmological topics, exploring properties of supernovae to
z~1, strong and weak lensing, the large-scale distribution of galaxies and
baryon oscillations, and how these different probes may be combined to
constrain cosmological models and the physics of dark energy.Comment: 596 pages. Also available at full resolution at
http://www.lsst.org/lsst/sciboo
Finishing the euchromatic sequence of the human genome
The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead
Telomerecat: A ploidy-agnostic method for estimating telomere length from whole genome sequencing data.
Telomere length is a risk factor in disease and the dynamics of telomere length are crucial to our understanding of cell replication and vitality. The proliferation of whole genome sequencing represents an unprecedented opportunity to glean new insights into telomere biology on a previously unimaginable scale. To this end, a number of approaches for estimating telomere length from whole-genome sequencing data have been proposed. Here we present Telomerecat, a novel approach to the estimation of telomere length. Previous methods have been dependent on the number of telomeres present in a cell being known, which may be problematic when analysing aneuploid cancer data and non-human samples. Telomerecat is designed to be agnostic to the number of telomeres present, making it suited for the purpose of estimating telomere length in cancer studies. Telomerecat also accounts for interstitial telomeric reads and presents a novel approach to dealing with sequencing errors. We show that Telomerecat performs well at telomere length estimation when compared to leading experimental and computational methods. Furthermore, we show that it detects expected patterns in longitudinal data, repeated measurements, and cross-species comparisons. We also apply the method to a cancer cell data, uncovering an interesting relationship with the underlying telomerase genotype
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