Disseny d'una web que exposi els treballs finals dels estudiants

Es proposa una web, que arreplegui tots els treballs i que al mateix temps els agrupi, que es puguin fer cerques fàcilment, haurà de ser dinàmica i agradable d'utilitzar.Se propone una web, que agrupe todos los trabajos i que se puedan realizar búsquedas fácilmente, tendrá que ser dinámica i agradable de utilizar.Proposal of a web that would assemble all projects and at the same time divide them into groups, so that searches would be easy to perform. The web should be dynamic and pleasant to use

The absence of the arabidopsis chaperone complex CAF-1 produces mitotic chromosome abnormalities and changes in the expression profiles of genes involved in DNA repair

Chromatin Assembly Factor 1 (CAF-1) is an evolutionary conserved heterotrimeric chaperone complex that facilitates the incorporation of histones H3 and H4 onto newly synthesized DNA. We demonstrate here that the mutant deficient for the large subunit of the complex, fas1-4, and in minor extent, the mutant deficient for the middle subunit, fas2-1, display chromosome abnormalities throughout Arabidopsis mitosis. Among them, we observed multicentromeric chromosomes at metaphase, and chromatid bridges and acentric fragments at anaphase-telophase. 45S rDNA and telomeric sequences were frequently involved in bridges and fragments. Gene expression analysis by real-time qPCR has revealed that several genes related to homologous recombination (HR) and alternative non-homologous end-joining (aNHEJ) are overexpressed in fas1-4. These results concur with previous studies which have indicated that HR may be involved in the progressive loss of 45S rDNA and telomeres displayed by fas mutants. However, increased expression of PARP1, PARP2, and LIG6 in fas1-4, and the phenotype shown by the double mutant fas1 rad51 suggest that aNHEJ should also be responsible for the chromosomal aberrations observed. The activity of different DNA repair pathways in absence of CAF-1 is discussed

Determining the nuclear neutron distribution from Coherent Elastic neutrino-Nucleus Scattering: current results and future prospects

Coherent elastic neutrino-nucleus scattering (CE$\nu$NS), a process recently measured for the first time at ORNL's Spallation Neutron Source, is directly sensitive to the weak form factor of the nucleus. The European Spallation Source (ESS), presently under construction, will generate the most intense pulsed neutrino flux suitable for the detection of CE$\nu$NS. In this paper we quantify its potential to determine the root mean square radius of the point-neutron distribution, for a variety of target nuclei and a suite of detectors. To put our results in context we also derive, for the first time, a constraint on this parameter from the analysis of the energy and timing data of the CsI detector at the COHERENT experiment.Comment: 16 pages, 4 figures. Minor text changes and references added. Matches version accepted by JHE

The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication

The GINS complex, originally discovered in Saccharomyces cerevisiae and Xenopus laevis, binds to DNA replication origins shortly before the onset of S phase and travels with the replication forks after initiation. In this study we present a detailed characterization of the human GINS (hGINS) homolog. Using new antibodies that allow the detection of endogenous hGINS in cells and tissues, we have examined its expression, abundance, subcellular localization and association with other DNA replication proteins. Expression of hGINS is restricted to actively proliferating cells. During the S phase, hGINS becomes part of a Cdc45–MCM–GINS (CMG) complex that is assembled on chromatin. Down-regulation of hGINS destabilizes CMG, causes a G1–S arrest and slows down ongoing DNA replication, effectively blocking cell proliferation. Our data support the notion that hGINS is an essential component of the human replisome

Determining the nuclear neutron distribution from Coherent Elastic neutrino-Nucleus Scattering: current results and future prospects

Coherent Elastic neutrino-Nucleus Scattering (CEνNS), a process recently measured for the first time at ORNL's Spallation Neutron Source, is directly sensitive to the weak form factor of the nucleus. The European Spallation Source (ESS), presently under construction, will generate the most intense pulsed neutrino flux suitable for the detection of CEνNS. In this paper we quantify its potential to determine the root mean square radius of the point-neutron distribution, for a variety of target nuclei and a suite of detectors. To put our results in context we also derive, for the first time, a constraint on this parameter from the analysis of the energy and timing data of the CsI detector at the COHERENT experiment

Using Player's Body-Orientation to Model Pass Feasibility in Soccer

Given a monocular video of a soccer match, this paper presents a computational model to estimate the most feasible pass at any given time. The method leverages offensive player's orientation (plus their location) and opponents' spatial configuration to compute the feasibility of pass events within players of the same team. Orientation data is gathered from body pose estimations that are properly projected onto the 2D game field; moreover, a geometrical solution is provided, through the definition of a feasibility measure, to determine which players are better oriented towards each other. Once analyzed more than 6000 pass events, results show that, by including orientation as a feasibility measure, a robust computational model can be built, reaching more than 0.7 Top-3 accuracy. Finally, the combination of the orientation feasibility measure with the recently introduced Expected Possession Value metric is studied; promising results are obtained, thus showing that existing models can be refined by using orientation as a key feature. These models could help both coaches and analysts to have a better understanding of the game and to improve the players' decision-making process.Comment: Accepted at the Computer Vision in Sports Workshop at CVPR 202

Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Atfas1-4 Mutant

Chromatin Assembly Factor 1 (CAF-1) is a histone chaperone that assembles acetylated histones H3/H4 onto newly synthesized DNA, allowing the de novo assembly of nucleosomes during replication. CAF-1 is an evolutionary conserved heterotrimeric protein complex. In Arabidopsis, the three CAF-1 subunits are encoded by FAS1, FAS2 and MSI1. Atfas1-4 mutants have reduced fertility due to a decrease in the number of cells that enter meiosis. Interestingly, the number of DNA double-strand breaks (DSBs), measured by scoring the presence of γH2AX, AtRAD51 and AtDMC1 foci, is higher than in wild-type (WT) plants, and meiotic recombination genes such AtCOM1/SAE2, AtBRCA1, AtRAD51 and AtDMC1 are overexpressed. An increase in DSBs in this mutant does not have a significant effect in the mean chiasma frequency at metaphase I, nor a different number of AtMLH1 nor AtMUS81 foci per cell compared to WT at pachytene. Nevertheless, this mutant does show a higher gene conversion (GC) frequency. To examine how an increase in DSBs influences meiotic recombination and synaptonemal complex (SC) formation, we analyzed double mutants defective for AtFAS1 and different homologous recombination (HR) proteins. Most showed significant increases in both the mean number of synapsis initiation points (SIPs) and the total length of AtZYP1 stretches in comparison with the corresponding single mutants. These experiments also provide new insight into the relationships between the recombinases in Arabidopsis, suggesting a prominent role for AtDMC1 versus AtRAD51 in establishing interhomolog interactions. In Arabidopsis an increase in the number of DSBs does not translate to an increase in the number of crossovers (COs) but instead in a higher GC frequency. We discuss different mechanisms to explain these results including the possible existence of CO homeostasis in plants

Molecular architecture of the human GINS complex

Chromosomal DNA replication is strictly regulated through a sequence of steps that involve many macromolecular protein complexes. One of these is the GINS complex, which is required for initiation and elongation phases in eukaryotic DNA replication. The GINS complex consists of four paralogous subunits. At the G1/S transition, GINS is recruited to the origins of replication where it assembles with cell-division cycle protein (Cdc)45 and the minichromosome maintenance mutant (MCM)2–7 to form the Cdc45/Mcm2–7/GINS (CMG) complex, the presumed replicative helicase. We isolated the human GINS complex and have shown that it can bind to DNA. By using single-particle electron microscopy and three-dimensional reconstruction, we obtained a medium-resolution volume of the human GINS complex, which shows a horseshoe shape. Analysis of the protein interactions using mass spectrometry and monoclonal antibody mapping shows the subunit organization within the GINS complex. The structure and DNA-binding data suggest how GINS could interact with DNA and also its possible role in the CMG helicase complex