13 research outputs found
Investigations of peptide structural stability in vacuo
Gas-phase analytical techniques provide very valuable tools for tackling the
structural complexity of macromolecular structures such as those encountered in
biological systems. Conformational dynamics of polypeptides and polypeptide
assemblies underlie most biological functionalities, yet great difficulties arise when
investigating such phenomena with the well-established techniques of X-ray
crystallography and NMR. In areas such as these ion mobility interfaced with mass
spectrometry (IMMS) and molecular modelling can make a significant contribution.
During an IMMS experiment analyte ions drift in a chamber filled with an inert gas;
measurement of the transport properties of analyte ions under the influence of a weak
electric field can lead to determination of the orientationally-averaged collision
cross-section of all resolved ionic species. A comparison with cross-sections
estimated for model molecular geometries can lead to structural assignments. Thus
IMMS can be used effectively to separate gas-phase ions based on their
conformation. The drift tube employed in the experiments described herein is
thermally regulated, which also enables the determination of collision cross-sections
over a range of temperatures, and can provide a view of temperature-dependent
conformational dynamics over the experimental (low microsecond) timescale.
Studies described herein employ IMMS and a gamut of other MS-based techniques,
solution spectroscopy and â importantly â molecular mechanics simulations to assess
a) conformational stability of isolated peptide ions, with a focus on small model
peptides and proteins, especially the Trp cage miniprotein; and b) structural
characteristics of oligomeric aggregates of an amyloidogenic peptide.
The results obtained serve to clarify the factors which dominate the intrinsic stability
of non-covalent structure in isolated peptides and peptide assemblies. Strong
electrostatic interactions are found to play a pivotal role in determining the
conformations of isolated proteins. Secondary structures held together by hydrogen
bonding, such as helices, are stable in the absence of solvent, however gas-phase
protein structures display loss of their hydrophobic cores. The absence of a polar
solvent, âself-solvationâ is by far the most potent force influencing the gas-phase configuration of these systems. Geometries that are more compact than the folded
state observed in solution are routinely detected, indicating the existence of
intrinsically stable compact non-native states in globular proteins, illuminating the
nature of proteinsâ âunfoldedâ states
Peptide Fragments of a β-Defensin Derivative with Potent Bactericidal Activity
β-Defensins are known to be both antimicrobial and able to chemoattract various immune cells. Although the sequences of paralogous genes are not highly conserved, the core defensin structure is retained. Defb14-1C(V) has bactericidal activity similar to that of its parent peptide (murine β-defensin Defb14) despite all but one of the canonical six cysteines being replaced with alanines. The 23-amino-acid N-terminal half of Defb14-1C(V) is a potent antimicrobial while the C-terminal half is not. Here, we use a library of peptide derivatives to demonstrate that the antimicrobial activity can be localized to a particular region. Overlapping fragments of the N-terminal region were tested for their ability to kill Gram-positive and Gram-negative bacteria. We demonstrate that the most N-terminal fragments (amino acids 1 to 10 and 6 to 17) are potent antimicrobials against Gram-negative bacteria whereas fragments based on sequence more C terminal than amino acid 13 have very poor activity against both Gram-positive and -negative types. We further test a series of N-terminal deletion peptides in both their monomeric and dimeric forms. We find that bactericidal activity is lost against both Gram types as the deletion region increases, with the point at which this occurs varying between bacterial strains. The dimeric form of the peptides is more resistant to the peptide deletions, but this is not due just to increased charge. Our results indicate that the primary sequence, together with structure, is essential in the bactericidal action of this β-defensin derivative peptide and importantly identifies a short fragment from the peptide that is a potent bactericide
Early stages of insulin fibrillogenesis examined with ion mobility mass spectrometry and molecular modelling
A Kinetic Study of Ovalbumin Fibril Formation:The Importance of Fragmentation and End-Joining
AbstractThe ability to control the morphologies of biomolecular aggregates is a central objective in the study of self-assembly processes. The development of predictive models offers the surest route for gaining such control. Under the right conditions, proteins will self-assemble into fibers that may rearrange themselves even further to form diverse structures, including the formation of closed loops. In this study, chicken egg white ovalbumin is used as a model for the study of fibril loops. By monitoring the kinetics of self-assembly, we demonstrate that loop formation is a consequence of end-to-end association between protein fibrils. A model of fibril formation kinetics, including end-joining, is developed and solved, showing that end-joining has a distinct effect on the growth of fibrillar mass density (which can be measured experimentally), establishing a link between self-assembly kinetics and the underlying growth mechanism. These results will enable experimentalists to infer fibrillar morphologies from an appropriate analysis of self-assembly kinetic data
Conformational dynamics of alpha-synuclein:insights from mass spectrometry
The aggregation and deposition of alpha-synuclein in Lewy bodies is associated with the progression of Parkinson's disease. Here, Mass Spectrometry (MS) is used in combination with Ion Mobility (IM), chemical crosslinking and Electron Capture Dissociation (ECD) to probe transient structural elements of alpha-synuclein and its oligomers. Each of these reveals different aspects of the conformational heterogeneity of this 14 kDa protein. IM-MS analysis indicates that this protein is highly disordered, presenting in positive ionisation mode with a charge state range of 5 <= z <= 21 for the monomer, along with a collision cross section range of similar to 1600 angstrom(2)). Chemical crosslinking applied in conjunction with IM-MS captures solution phase conformational families enabling comparison with those exhibited in the gas phase. Crosslinking IM-MS identifies 3 distinct conformational families, Compact (similar to 1200 angstrom(2)), Extended (similar to 1500 angstrom(2)) and Unfolded (similar to 2350 angstrom(2)) which correlate with those observed in solution. ECD-Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry (ECD-FT-ICR MS) highlights the effect of pH on alpha-synuclein structure, identifying the conformational flexibility of the N and C termini as well as providing evidence for structure in the core and at times the C terminus. A hypothesis is proposed for the variability displayed in the structural rearrangement of alpha-synuclein following changes in solution pH. Following a 120 h aggregation time course, we observe an increase in the ratio of dimer to monomer, but no gross conformational changes in either, beyond the significant variations that are observed day-to-day from this conformationally dynamic protein
Investigations of peptide structural stability in vacuo
Gas-phase analytical techniques provide very valuable tools for tackling the structural complexity of macromolecular structures such as those encountered in biological systems. Conformational dynamics of polypeptides and polypeptide assemblies underlie most biological functionalities, yet great difficulties arise when investigating such phenomena with the well-established techniques of X-ray crystallography and NMR. In areas such as these ion mobility interfaced with mass spectrometry (IMMS) and molecular modelling can make a significant contribution. During an IMMS experiment analyte ions drift in a chamber filled with an inert gas; measurement of the transport properties of analyte ions under the influence of a weak electric field can lead to determination of the orientationally-averaged collision cross-section of all resolved ionic species. A comparison with cross-sections estimated for model molecular geometries can lead to structural assignments. Thus IMMS can be used effectively to separate gas-phase ions based on their conformation. The drift tube employed in the experiments described herein is thermally regulated, which also enables the determination of collision cross-sections over a range of temperatures, and can provide a view of temperature-dependent conformational dynamics over the experimental (low microsecond) timescale. Studies described herein employ IMMS and a gamut of other MS-based techniques, solution spectroscopy and â importantly â molecular mechanics simulations to assess a) conformational stability of isolated peptide ions, with a focus on small model peptides and proteins, especially the Trp cage miniprotein; and b) structural characteristics of oligomeric aggregates of an amyloidogenic peptide. The results obtained serve to clarify the factors which dominate the intrinsic stability of non-covalent structure in isolated peptides and peptide assemblies. Strong electrostatic interactions are found to play a pivotal role in determining the conformations of isolated proteins. Secondary structures held together by hydrogen bonding, such as helices, are stable in the absence of solvent, however gas-phase protein structures display loss of their hydrophobic cores. The absence of a polar solvent, âself-solvationâ is by far the most potent force influencing the gas-phase configuration of these systems. Geometries that are more compact than the folded state observed in solution are routinely detected, indicating the existence of intrinsically stable compact non-native states in globular proteins, illuminating the nature of proteinsâ âunfoldedâ states.EThOS - Electronic Theses Online ServiceGBUnited Kingdo
Effects of drift gas on collision cross sections of a protein standard in linear drift tube and traveling wave ion mobility mass spectrometry
Conformational landscapes of rigid and flexible molecules explored with variable temperature ion mobility-mass spectrometry
Understanding the effect of temperature to the structural integrity and dynamics of proteins has relevance for many areas including biotechnology and the maintenance of a stable food supply for the climate emergency. The methods that can explore changes in structure as a function of sub-ambient temperature are scant, and yet many drugs are stored at such temperatures. Here we show how variable temperature ion mobility-mass spectrometry (VT-IM-MS) can provide the role of temperature on conformational landscapes in the form of collision cross sections at discrete temperatures. To delineate collision effects from structural change we report measurements made on four molecules that possess different degrees of rigidity namely: poly (L-lysine) (PLL) dendrimer, ubiquitin, β-casein and ι-synuclein from 190-350K. We show that the PLL dendrimer varies with temperature consistent with collision theory, and conclude, as expected, its structure does not alter significantly over this range. By contrast, the structure of each protein is altered by the temperature of the drift gas, with notable unfolding to all charge states at 350 K and also at 250 K, following predicted in vitro stability curves, and with conformational variation that gives qualitative insights to the effect of temperature on the free energy landscape of these proteins. We also show that we can kinetically trap unfolding intermediates at drift temperatures of 210 K and 190 K on a millisecond experimental time scale. For alpha-synuclein, the 13+ ions present two distinct conformers and VT-IM-MS measurements allow us to calculate the transition rate and activation energies for conversion between these. These data exemplify the capability of VT-IM-MS to provide insights to thermodynamics involved in conformational restructuring.