Analysis of clothing microclimate conditions for lower body part workwear dedicated for motor impaired employees

Workwear dedicated for motor impaired employees was designed. The woven fabrics used meet the requirements for light to moderate effort according to PN-P-08525:1998, reaching suitable level of water vapor resistance meanwhile. The workwear’s design was carefully chosen to meet the requirements of motor impaired employees during their work activities. The analysis of microclimate was carried out to investigate the garment’s ability to transport water vapor outside from gaps between body part and item of clothing. Two sets of workwear were tested, showing that both garments’ construction and woven fabrics properties influence thereon

Thioloxidoreductase HP0231 of Helicobacter pylori impacts HopQ-dependent CagA translocation

Thioloxidoreductase HP0231 of Helicobacter pylori plays essential roles in gastric colonization and related gastric pathology. Comparative proteomics and analysis of complexes between HP0231 and its protein substrates suggested that several Hop proteins are its targets. HP0231 is a dimeric oxidoreductase that functions in an oxidizing Dsb (disulfide bonds) pathway of H. pylori. H. pylori HopQ possesses six cysteine residues, which generate three consecutive disulfide bridges. Comparison of the redox state of HopQ in wild-type cells to that in hp0231-mutated cells clearly indicated that HopQ is a substrate of HP0231. HopQ binds CEACAM1, 3, 5 and 6 (carcinoembryonic antigen-related cell adhesion molecules). This interaction enables T4SS-mediated translocation of CagA into host cells and induces host signaling. Site directed mutagenesis of HopQ (changing cysteine residues into serine) and analysis of the functioning of HopQ variants showed that HP0231 influences the delivery of CagA into host cells, in part through its impact on HopQ redox state. Introduction of a C382S mutation into HopQ significantly affects its reaction with CEACAM receptors, which disturbs T4SS functioning and CagA delivery. An additional effect of HP0231 on other adhesins and their redox state, resulting in their functional impairment, cannot be excluded

Identification of genetic variants associated with Huntington's disease progression: a genome-wide association study

Background Huntington's disease is caused by a CAG repeat expansion in the huntingtin gene, HTT. Age at onset has been used as a quantitative phenotype in genetic analysis looking for Huntington's disease modifiers, but is hard to define and not always available. Therefore, we aimed to generate a novel measure of disease progression and to identify genetic markers associated with this progression measure. Methods We generated a progression score on the basis of principal component analysis of prospectively acquired longitudinal changes in motor, cognitive, and imaging measures in the 218 indivduals in the TRACK-HD cohort of Huntington's disease gene mutation carriers (data collected 2008–11). We generated a parallel progression score using data from 1773 previously genotyped participants from the European Huntington's Disease Network REGISTRY study of Huntington's disease mutation carriers (data collected 2003–13). We did a genome-wide association analyses in terms of progression for 216 TRACK-HD participants and 1773 REGISTRY participants, then a meta-analysis of these results was undertaken. Findings Longitudinal motor, cognitive, and imaging scores were correlated with each other in TRACK-HD participants, justifying use of a single, cross-domain measure of disease progression in both studies. The TRACK-HD and REGISTRY progression measures were correlated with each other (r=0·674), and with age at onset (TRACK-HD, r=0·315; REGISTRY, r=0·234). The meta-analysis of progression in TRACK-HD and REGISTRY gave a genome-wide significant signal (p=1·12 × 10−10) on chromosome 5 spanning three genes: MSH3, DHFR, and MTRNR2L2. The genes in this locus were associated with progression in TRACK-HD (MSH3 p=2·94 × 10−8 DHFR p=8·37 × 10−7 MTRNR2L2 p=2·15 × 10−9) and to a lesser extent in REGISTRY (MSH3 p=9·36 × 10−4 DHFR p=8·45 × 10−4 MTRNR2L2 p=1·20 × 10−3). The lead single nucleotide polymorphism (SNP) in TRACK-HD (rs557874766) was genome-wide significant in the meta-analysis (p=1·58 × 10−8), and encodes an aminoacid change (Pro67Ala) in MSH3. In TRACK-HD, each copy of the minor allele at this SNP was associated with a 0·4 units per year (95% CI 0·16–0·66) reduction in the rate of change of the Unified Huntington's Disease Rating Scale (UHDRS) Total Motor Score, and a reduction of 0·12 units per year (95% CI 0·06–0·18) in the rate of change of UHDRS Total Functional Capacity score. These associations remained significant after adjusting for age of onset. Interpretation The multidomain progression measure in TRACK-HD was associated with a functional variant that was genome-wide significant in our meta-analysis. The association in only 216 participants implies that the progression measure is a sensitive reflection of disease burden, that the effect size at this locus is large, or both. Knockout of Msh3 reduces somatic expansion in Huntington's disease mouse models, suggesting this mechanism as an area for future therapeutic investigation

Att leva på sin (social)kompetens : - en kvalitativ studie om arbetsmiljön i IT-konsultbranschen

Den här magisteruppsatsen behandlar mänskliga processer i konsultbranschen inom affärsutveckling. Författaren undersöker hur kontakter med kunder samt relationer inom konsultföretagen påverkar arbetsmiljön i konsultbranschen. Syftet med undersökningen är att på nytt skapa en bild av konsultyrket och de förhållandena som påverkar konsulternas vardagliga arbete och arbetsmiljön i branschen. Därför baserar sig undersökningen på en Grundad Teori-ansats och byggs upp på en kvalitativ forskningsmetodik. Studien omfattar semi-strukturerade intervjuer med 12 konsulter och 3 projektledare inom olika företag i affärsutvecklingsbranschen. Undersökningens resultat jämförs kort med teorier vilka studerades i början av forskningsarbetet och med den tidigare forskningen i ämnet. I själva undersökningen tar man däremot avstånd från det som har redan sagts inom konsultyrkets problematik. Från analysen av undersökningens resultat framgår det på den organisatoriska nivån att: - Socialkompetens och kommunikation är nyckelegenskaperna i konsultyrket. - Det finns brist på samarbete inom konsultföretagen. - Det fattas tid för vidareutbildning och kunskapsöverföring inom företagen. - Faktureringskraven är vanligtviss för höga och påverkar ofta konsulternas privatliv och familj. Analysen gav att följande gäller för konsulterna: - Konsulter drivs av att lära sig nytt, lösa problem (gärna med andra), se sina lösningar fungera och underlätta kundernas processer. - Konsulterna uppskattar friheten i arbetet, omväxlingen och variationen av uppdrag och människor de möts. - Samtidigt behöver konsulter rutiner och standardisering av processer i sitt vardagliga arbete. - De själva ställer höga krav på sig själva

Effect of targeted vs standard fortification of breast milk on growth and development of preterm infants (<= 32weeks) : study protocol for a randomized controlled trial

Background: Human milk is recommended for all very low birth weight infants. Breastmilk is highly variable in nutrient content, failing to meet the nutritional demands of this group. Fortification of human milk is recommended to prevent extrauterine growth retardation and associated poor neurodevelopmental outcome. However, standard fortification with fixed dose multicomponent fortifier does not account for the variability in milk composition. Targeted fortification is a promising alternative and needs further investigation. Methods: This randomized controlled trial will recruit preterm infants (≤ 32 weeks of gestation) within the first 7 days of life. After reaching 80 ml/kg/day of enteral feeding, patients will be randomized to receive standard fortification (HMF, Nutricia) or targeted fortification (modular components: Bebilon Bialka, Nutricia—protein; Polycal, Nutricia—carbohydrates; Calogen, Nutricia—lipids). The intervention will continue until 37 weeks of post-conception age or hospital discharge. Parents and outcome assessors will be blinded to the intervention. The primary outcome measure is velocity of weight, length, and head growth until 36 weeks post-conceptional age or discharge. Secondary outcomes include neurodevelopment at 12 months assessed with Bayley Scale of Development III, repeated at 36 months; body composition at discharge and at 4 months; and incidence of necrotizing enterocolitis, sepsis, retinopathy of prematurity, and bronchopulmonary dysplasia. Discussion: Targeted fortification has previously been shown as doable in the neonatal intensive care unit context. If it shows to improve growth and neonatal outcome, choosing the targeted fortification as a first line nutritional approach in very low birth weight infants may become a recommendation. Trial registration: ClinicalTrials.govNCT03775785, Registered on July 2019

The morphology, clinical significance and imaging methods of the atrial septal pouch: A critical review

The purpose of this study was to present current knowledge about the atrial septal pouch, which is a relatively new anatomical discovery located within the human interatrial septum of the heart. The atrial septal pouch is formed during the postnatal partial fusion of the primary and secondary septum. The prevalence of the left septal pouch is 40.8%, the right septal pouch is present in 5.1% and a double pouch is seen in 3.7% of cases. The patent foramen ovale channel is present in 25.9% of all cases. The left septal pouch is a relatively small structure with an average depth of 8.4 ± 5.1 mm and mean volume of 0.31 ± 0.11 ml. The right septal pouch is significantly smaller than the left septal pouch, with a mean depth of 6.2 ± 3.4 mm and mean volume of 0.25 ± 0.08 ml. Both transesophageal echocardiography and cardiac computed tomography are useful techniques for detecting left septal pouches with the insignificant lower prevalence of detecting left septal pouches compared to the matched postmortem material. Transesophageal echocardiography with contrast modality seems to be superior than computed tomography due to its unambiguous patent foramen ovale channel detection and it should be the preferred imaging technique in clinical identification of septal pouches. The anatomy of left septal pouch may promote blood stasis and thrombus formation. Case reports and cross-sectional, single-center studies show that there is an association between the presence of a left sided septal pouch and an increased risk for cryptogenic stroke. Moreover, the left septal pouch may be an arrhythmogenic substrate responsible for triggering atrial fibrillation. The right septal pouch seems to have no clinical significance. In summary, the left septal pouch is the most common structure found within the interatrial septum and should be considered as a normal variant of the human heart anatomy. The morphological nature of the atrial septal pouch is well known, however, more clinical research is needed to resolve its possible connection with atrial fibrillation and cardio-embolic stroke. Keywords: Interatrial septum, Atrium, Ischemic stroke, Cardioembolic stroke, Patent foramen ovale, Atrial fibrillation, Thrombosi

The Musical Instruments in the Early Vernacular Translations of the Psalms (3): Collective Research

International audienc