Differentiation of Self: A Validation Study of the Bowen Theory Construct

The Differentiation of Self Inventory-Revised (DSI-R

Differentiation of Self: A Validation Study of the Bowen Theory Construct

The Differentiation of Self Inventory-Revised (DSI-R

D9.8: Final Report on Provision of Staging Support

This document presents an extension to the service architecture proposed in the EUDAT deliverable D9.4 [EUDAT D9.4] that enables a user to get easy access to different storage systems such as IBM TSM (see glossary for abbreviations) or HPSS. Hereby the document focuses on features for integrity checking based on check- summing and scheduling of staging requests to achieve the best possible quality of service. The architecture provides a front-end API following the REST paradigm over HTTP. Beside the features presented in the previous deliverable, the architecture is now extended to schedule and reorder slow, asynchronous operations based on predefined strategies. The architecture was prototypically implemented to work within the B2SAFE context and was successfully tested in defined testing environments

Exploring virtue ethics in psychodynamic psychotherapy: latent changes in humility, affect regulation, symptoms, and well-being

[Empirical exploration of a salutary role for virtues on both mental health symptoms and well-being has increased. Yet, tests for this role in psychotherapy have not matched research in other contexts. As such, we tested the virtue ethics premise that growth in humility could facilitate changes in symptoms and well-being in the context of contemporary relational psychotherapy (CRP; Sandage et al., 2020). CRP is grounded in three premises: (a) conceptualizing clients within their historical, familial and sociocultural contexts, (b) understanding distress as stemming from maladaptive intra- and interpersonal patterns, and (c) prioritizing a here-and-now focus within the therapeutic relationship. We proposed experiential avoidance as the mechanism whereby humility influences symptoms and well-being.]First author draf

Amino Acid Deprivation Promotes Tumor Angiogenesis through the GCN2/ATF4 Pathway

AbstractAs tumors continue to grow and exceed their blood supply, nutrients become limited leading to deficiencies in amino acids (AAD), glucose (GD), and oxygen (hypoxia). These alterations result in significant changes in gene expression. While tumors have been shown to overcome the stress associated with GD or hypoxia by stimulating vascular endothelial growth factor (VEGF)-mediated angiogenesis, the role of AAD in tumor angiogenesis remains to be elucidated. We found that in human tumors, the expression of the general control non-derepressible 2 (GCN2, an AAD sensor) kinase is elevated at both protein and mRNA levels. In vitro studies revealed that VEGF expression is universally induced by AAD treatment in all five cell lines tested (five of five). This is in contrast to two other angiogenesis mediators interleukin-6 (two of five) and fibroblast growth factor 2 (two of five) that have a more restricted expression. Suppressing GCN2 expression significantly decreased AAD-induced VEGF expression. Silencing activating transcription factor 4 (ATF4), a downstream transcription factor of the GCN2 signaling pathway, is also associated with strong inhibition of AAD-induced VEGF expression. PKR-like kinase, the key player in GD-induced unfolded protein response is not involved in this process. In vivo xenograft tumor studies in nonobese diabetic/severe combined immunodeficient mice confirmed that knockdown of GCN2 in tumor cells retards tumor growth and decreases tumor blood vessel density. Our results reveal that the GCN2/ATF4 pathway promotes tumor growth and angiogenesis through AAD-mediated VEGF expression and, thus, is a potential target in cancer therapy

Qué hacer con las separatas

Sección: Pequeñas soluciones para grandes problemasLas separatas son unidades de informacion muy útiles y de gran valor para los investigadores pero que, a menudo, resultan una rémora pues su materia no encaja bien en nuestra biblioteca.N

Mental health, well-being, and experiences of the COVID-19 pandemic: a mixed methods practice-based study

First author draf

The correlation between reading and mathematics ability at age twelve has a substantial genetic component

Dissecting how genetic and environmental influences impact on learning is helpful for maximizing numeracy and literacy. Here we show, using twin and genome-wide analysis, that there is a substantial genetic component to children’s ability in reading and mathematics, and estimate that around one half of the observed correlation in these traits is due to shared genetic effects (so-called Generalist Genes). Thus, our results highlight the potential role of the learning environment in contributing to differences in a child’s cognitive abilities at age twelve

The stem cell organisation, and the proliferative and gene expression profile of Barrett's epithelium, replicates pyloric-type gastric glands

Objective: Barrett's oesophagus shows appearances described as ‘intestinal metaplasia’, in structures called ‘crypts’ but do not typically display crypt architecture. Here, we investigate their relationship to gastric glands. Methods: Cell proliferation and migration within Barrett's glands was assessed by Ki67 and iododeoxyuridine (IdU) labelling. Expression of mucin core proteins (MUC), trefoil family factor (TFF) peptides and LGR5 mRNA was determined by immunohistochemistry or by in situ hybridisation, and clonality was elucidated using mitochondrial DNA (mtDNA) mutations combined with mucin histochemistry. Results: Proliferation predominantly occurs in the middle of Barrett's glands, diminishing towards the surface and the base: IdU dynamics demonstrate bidirectional migration, similar to gastric glands. Distribution of MUC5AC, TFF1, MUC6 and TFF2 in Barrett's mirrors pyloric glands and is preserved in Barrett's dysplasia. MUC2-positive goblet cells are localised above the neck in Barrett's glands, and TFF3 is concentrated in the same region. LGR5 mRNA is detected in the middle of Barrett's glands suggesting a stem cell niche in this locale, similar to that in the gastric pylorus, and distinct from gastric intestinal metaplasia. Gastric and intestinal cell lineages within Barrett's glands are clonal, indicating derivation from a single stem cell. Conclusions: Barrett's shows the proliferative and stem cell architecture, and pattern of gene expression of pyloric gastric glands, maintained by stem cells showing gastric and intestinal differentiation: neutral drift may suggest that intestinal differentiation advances with time, a concept critical for the understanding of the origin and development of Barrett's oesophagus

Genome-wide association study identifies a variant in HDAC9 associated with large vessel ischemic stroke

Genetic factors have been implicated in stroke risk but few replicated associations have been reported. We conducted a genome-wide association study (GWAS) in ischemic stroke and its subtypes in 3,548 cases and 5,972 controls, all of European ancestry. Replication of potential signals was performed in 5,859 cases and 6,281 controls. We replicated reported associations between variants close to PITX2 and ZFHX3 with cardioembolic stroke, and a 9p21 locus with large vessel stroke. We identified a novel association for a SNP within the histone deacetylase 9(HDAC9) gene on chromosome 7p21.1 which was associated with large vessel stroke including additional replication in a further 735 cases and 28583 controls (rs11984041, combined P = 1.87×10−11, OR=1.42 (95% CI) 1.28-1.57). All four loci exhibit evidence for heterogeneity of effect across the stroke subtypes, with some, and possibly all, affecting risk for only one subtype. This suggests differing genetic architectures for different stroke subtypes