The Genetic Basis of Susceptibility to Therapy-related Leukemia in Mice

Treatment-related acute myeloid leukemia (t-AML) arises as a result of treating primary malignancies with alkylator chemotherapy drugs and has a poor prognosis. Genetic background influences the risk of acquiring t-AML, yet little is known about susceptibility factors. To identify candidate risk factors, cohorts of twenty inbred mouse strains were treated with N-ethyl-N-nitrosourea (ENU), a potent alkylating agent in mice. Six of these mouse strains were susceptible to alkylator-induced leukemia. SWR/J mice were the most susceptible in this relatively small screen. We expanded on that study to characterize SWR/J mice as a susceptible strain to t-AML using 245 mice. Mice were treated with different permutations of steroid treatment to determine the effect they would have on leukemia numbers. In addition to the susceptible strains, quantitative trait loci mapping was used on the initial study to identify genetic components responsible for the leukemic phenotype. This analysis revealed two significant peaks of interest on chromosomes 3 and 14. The 1 Mb region on chromosome 3 contains six genes, one of which was myeloid leukemia factor 1 (Mlf1). In humans, MLF1 waspreviously identified for its involvement in a chromosomal translocation with nucleophosmin (NPM) that is restricted to AML and myelodysplastic (MDS) patients. We show that MLF1 is pro-apoptotic and decreases the viability of hematopoietic cells

Middle school natural disaster inquiry unit with scaffolding

Inquiry based learning is complex and challenging. In order to maximize its benefits, it is vital to understand what teaching methods and scaffolds best support different student populations as they are introduced to inquiry-based learning in different contexts. One major challenge of inquiry is organizational: planning the process and organizing the information. This project studied the effectiveness of using a particular scaffolding tool, an advanced organizer template, to support seventh grade science students in an introductory inquiry-based unit. One group of seventh grade students used the advanced organizer template and one group did not. Students chose the type of project to create and their partner. Previous performance in their science class, the type of project they chose, and whether or not they used the Advanced Organizer Template were all statistically significant predictors of their success in the project. The data collected and observations made helped to develop the final Natural Disaster Unit

The Role of Life Experiences on Perceptions of Leisure During Adulthood: A Longitudinal Analysis

19 pagesThis study examined the life experiences of adults and their effect on perceptions of leisure. Data for this study came from A Study of Leisure during Adulthood (ASOLDA), a 10-year study of the values, attitudes, and perceived freedom in leisure of 84 adults. Individual growth curve modeling was used to explore patterns and change within leisure domains for the sample. Life structure predicted adults’ perceived freedom in leisure while life events were predictive of adults’ leisure attitudes. Data from interviews were used to supplement the survey data to provide a better understanding of the predictors of leisure perceptions in this study

The Rewiring of Ubiquitination Targets in a Pathogenic Yeast Promotes Metabolic Flexibility, Host Colonization and Virulence

Funding: This work was funded by the European Research Council [http://erc.europa.eu/], AJPB (STRIFE Advanced Grant; C-2009-AdG-249793). The work was also supported by: the Wellcome Trust [www.wellcome.ac.uk], AJPB (080088, 097377); the UK Biotechnology and Biological Research Council [www.bbsrc.ac.uk], AJPB (BB/F00513X/1, BB/K017365/1); the CNPq-Brazil [http://cnpq.br], GMA (Science without Borders fellowship 202976/2014-9); and the National Centre for the Replacement, Refinement and Reduction of Animals in Research [www.nc3rs.org.uk], DMM (NC/K000306/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgments We thank Dr. Elizabeth Johnson (Mycology Reference Laboratory, Bristol) for providing strains, and the Aberdeen Proteomics facility for the biotyping of S. cerevisiae clinical isolates, and to Euroscarf for providing S. cerevisiae strains and plasmids. We are grateful to our Microscopy Facility in the Institute of Medical Sciences for their expert help with the electron microscopy, and to our friends in the Aberdeen Fungal Group for insightful discussions.Peer reviewedPublisher PD

Village of Cobb

22 p.The purpose of this study was to gather opinions regarding issues to be considered in the development of the Village of Cobb's comprehensive plan. This summary is one of three summaries included in the Southwest Wisconsin Regional Planning Commission (SWWRPC) Comprehensive Planning project

Village of Rewey

22 p.The purpose of this study was to gather opinions regarding issues to be considered in the development of the Village of Rewey's comprehensive plan. This summary is one of three summaries included in the Southwest Wisconsin Regional Planning Commission (SWWRPC) Comprehensive Planning project

OUTDOOR LEISURE, PHYSICAL ACTIVITY, AND EATING HABITS AMONG COLLEGE STUDENTS: AN OBSERVATIONAL STUDY

BACKGROUND: Engagement in physical activity, outdoor leisure, and healthful eating habits are imperative for overall well-being. The purpose of the investigation was to examine physical activity, outdoor leisure, and eating habits to further our efforts in the Exercise is Medicine On-Campus (EIM-OC) initiative. METHODS: College students (n = 173) were recruited and completed a confidential online self-reported survey consisting of four questionnaires: Physical Activity and Outdoor Leisure Questionnaire, Barriers to Outdoor and Physical Activities, Motivators to Outdoor and Physical Activities, and the Eating Attitudes Test (EAT-26). RESULTS: The Physical Activity and Outdoor Leisure questionnaire (n = 120) responses revealed 86% of students were aware of the health benefits associated with physical activity. Despite awareness of the benefits, only 65% reported meeting the aerobic physical activity guidelines each week (150 min·wk-1 of moderate-intensity or 75 min·wk-1 of vigorous intensity exercise). Responses indicated 65% of students participated in at least 20 minutes of outdoor physical activity and 56% spent time outdoors at least 2-3 times each week. Lack of time, weather, and exposure to outdoor pests were the top reported barriers to participation in outdoor leisure. Students reported their top motivators were stress reduction, keeping physically fit, and getting away from the usual demands. EAT-26 responses revealed 13% of students presented with a score of 20 or higher, indicating a high level of concern about problematic eating behaviors. No significant differences were observed between students majoring in exercise science when compared to other majors (p = 0.174). However, disordered eating was significantly greater in students who were not athletes (p = 0.022). CONCLUSIONS: Students demonstrated a reasonable level of awareness regarding the advantages of adhering to physical activity guidelines. However, there remains a significant scope for enhancing their compliance with these recommendations and allocating more time to outdoor leisure activities. Furthermore, the noteworthy occurrence of disordered eating habits among non-athletic students is a cause for concern, suggesting a potential necessity for comprehensive on-campus educational initiatives aimed at promoting healthy eating patterns across all student populations

The Effects of the A Matter of Balance Program on Falls and Physical Risk of Falls, Tampa, Florida, 2013

Extracted text; Introduction This study investigated the effects of the A Matter of Balance (MOB) program on falls and physical risk factors of falling among community-dwelling older adults living in Tampa, Florida, in 2013. Methods A total of 110 adults (52 MOB, 58 comparison) were enrolled in this prospective cohort study. Data on falls, physical risk of falling, and other known risk factors of falling were collected at baseline and at the end of the program. Multivariate analysis of covariance with repeated measures and logistic regressions were used to investigate the effects of this program. Results Participants in the MOB group were less likely to have had a fall and had significant improvements in their physical risk of falling compared with adults in the comparison group. No significant effects of the MOB program on recurrent falls or the number of falls reported were found. Conclusion This study contributes to our understanding of the MOB program and its effectiveness in reducing falls and the physical risk of falling among older adults. The findings support extended use of this program to reduce falls and physical risk of falling among older adults

Cultural adaptations to augment health and mental health services: a systematic review

Background: Membership in diverse racial, ethnic, and cultural groups is often associated with inequitable health and mental health outcomes for diverse populations. Yet, little is known about how cultural adaptations of standard services affect health and mental health outcomes for service recipients. This systematic review identified extant themes in the research regarding cultural adaptations across a broad range of health and mental health services and synthesized the most rigorous experimental research available to isolate and evaluate potential efficacy gains of cultural adaptations to service delivery. Methods: MEDLINE, PsycINFO, CINAHL, EMBASE, and grey literature sources were searched for English-language studies published between January 1955 and January 2015. Cultural adaptations to any aspect of a service delivery were considered. Outcomes of interest included changes in service provider behavior or changes in the behavioral, medical, or self-reported experience of recipients. Results: Thirty-one studies met the inclusion criteria. The most frequently tested adaptation occurred in preventive services and consisted of modifying the content of materials or services delivered. None of the included studies focused on making changes in the provider’s behavior. Many different populations were studied but most research was concerned with the experiences and outcomes of African Americans. Seventeen of the 31 retained studies observed at least one significant effect in favor of a culturally adapted service. However there were also findings that favored the control group or showed no difference. Researchers did not find consistent evidence supporting implementation of any specific type of adaptation nor increased efficacy with any particular cultural group. Conclusions: Conceptual frameworks to classify cultural adaptations and their resultant health/mental health outcomes were developed and applied in a variety of ways. This review synthesizes the most rigorous research in the field and identifies implications for policy, practice, and research, including individualization, cost considerations, and patient or client satisfaction, among others.Other UBCNon UBCReviewedFacult