Genome-wide mapping targets of the metazoan chromatin remodeling factor NURF reveals nucleosome remodeling at enhancers, core promoters and gene insulators

NURF is a conserved higher eukaryotic ISWI-containing chromatin remodeling complex that catalyzes ATP-dependent nucleosome sliding. By sliding nucleosomes, NURF is able to alter chromatin dynamics to control transcription and genome organization. Previous biochemical and genetic analysis of the specificity-subunit of Drosophila NURF (Nurf301/Enhancer of Bithorax (E(bx)) has defined NURF as a critical regulator of homeotic, heat-shock and steroid-responsive gene transcription. It has been speculated that NURF controls pathway specific transcription by co-operating with sequence-specific transcription factors to remodel chromatin at dedicated enhancers. However, conclusive in vivo demonstration of this is lacking and precise regulatory elements targeted by NURF are poorly defined. To address this, we have generated a comprehensive map of in vivo NURF activity, using MNase-sequencing to determine at base pair resolution NURF target nucleosomes, and ChIP-sequencing to define sites of NURF recruitment. Our data show that, besides anticipated roles at enhancers, NURF interacts physically and functionally with the TRF2/DREF basal transcription factor to organize nucleosomes downstream of active promoters. Moreover, we detect NURF remodeling and recruitment at distal insulator sites, where NURF functionally interacts with and co-localizes with DREF and insulator proteins including CP190 to establish nucleosome-depleted domains. This insulator function of NURF is most apparent at subclasses of insulators that mark the boundaries of chromatin domains, where multiple insulator proteins co-associate. By visualizing the complete repertoire of in vivo NURF chromatin targets, our data provide new insights into how chromatin remodeling can control genome organization and regulatory interactions

Improving ionic conductivity of von-Alpen-type NASICON ceramic electrolytes via magnesium doping

NASICON (sodium (Na) superionic conductor) compounds have attracted considerable attention as promising solid electrolyte materials for advanced Na-based batteries. In this study, we investigated the improvement in ionic conductivities of von-Alpen-type NASICON (vA-NASICON) ceramic electrolytes by introducing a magnesium ion (Mg2+) as a heterogeneous element. The optimal Mg-doped vA-NASICON exhibited a high ionic conductivity of 3.64×10−3 S·cm−1, which was almost 80% higher than that of un-doped vA-NASICON. The changes in physicochemical properties of the vA-NASICONs through the Mg introduction were systematically analyzed, and their effects on the ionic conductivities of the vA-NASICON were studied in detail. When the optimal ratio of Mg2+ was used in a synthetic process, the relative density (96.6%) and grain boundary ionic conductivity (σgb) were maximized, which improved the total ionic conductivity (σt) of the vA-NASICON. However, when Mg2+ was introduced in excess, the ionic conductivity decreased because of the formation of an undesired sodium magnesium phosphate (NaxMgyPO4) secondary phase. The results of this study are expected to be effectively applied in the development of advanced sodium-based solid electrolytes with high ionic conductivities

Simple scheme for expanding a polarization-entangled W state by adding one photon

We propose a simple scheme for expanding a polarization-entangled W state. By mixing a single photon and one of the photons in an n-photon W state at a polarization-dependent beam splitter (PDBS), we can obtain an (n+1)-photon W state after post-selection. Our scheme also opens the door for generating n-photon W states using single photons and linear optics.Comment: 3 pages, 2 figure

Control of Interface Defects for Efficient and Stable Quasi-2D Perovskite Light-Emitting Diodes Using Nickel Oxide Hole Injection Layer.

Metal halide perovskites (MHPs) have emerged as promising materials for light-emitting diodes owing to their narrow emission spectrum and wide range of color tunability. However, the low exciton binding energy in MHPs leads to a competition between the trap-mediated nonradiative recombination and the bimolecular radiative recombination. Here, efficient and stable green emissive perovskite light-emitting diodes (PeLEDs) with an external quantum efficiency of 14.6% are demonstrated through compositional, dimensional, and interfacial modulations of MHPs. The interfacial energetics and optoelectronic properties of the perovskite layer grown on a nickel oxide (NiO x ) and poly(3,4-ethylenedioxythiophene):polystyrene sulfonate hole injection interfaces are investigated. The better interface formed between the NiO x /perovskite layers in terms of lower density of traps/defects, as well as more balanced charge carriers in the perovskite layer leading to high recombination yield of carriers are the main reasons for significantly improved device efficiency, photostability of perovskite, and operational stability of PeLEDs

A childhood case of spinal tuberculosis misdiagnosed as muscular dystrophy

Tuberculosis is primarily a pulmonary disease, but extra-pulmonary manifestations are not uncommon, especially in children and adolescents. Ten percent of extra pulmonary tuberculosis localizes to the bones and joints, and 56% of such cases affect the spine. We treated a childhood case of spinal tuberculosis misdiagnosed as muscular dystrophy in a patient without specific constitutional symptoms. We report this case because the patient had an unusual presentation of spinal tuberculosis

A Competência em Informação (Coinfo) na perspectiva da educação inclusiva

Tese (doutorado)—Universidade de Brasília, Faculdade de Ciência da Informação, Programa de Pós-Graduação em Ciência da Informação, 2017.Discute os desafios da sociedade da informação no que tange à promoção e garantia de uma educação inclusiva a partir de uma agenda estabelecida em ambiências supranacionais, internacionais e multilaterais. Destaca a necessidade de formação de professores para lidar com necessidades informacionais emergentes da prática educativa, em especial quando aplicada a pessoas com deficiência. Neste contexto, situa nos aspectos interdisciplinares entre Ciência da Informação e Educação, contribuições relacionadas tanto à inclusão quanto à atuação de docentes da Educação Básica. Admite os pressupostos teóricos e práticos da temática Competência em Informação – termo adotado para o conceito de Information Literacy – como alternativa de formação de docentes da Educação Básica na perspectiva de uma educação inclusiva. Propõe a utilização de um percurso metodológico baseado nas contribuições de estudos sobre o Paradigma Indiciário de Ginzburg o qual é composto por uma fase exploratória e uma fase aplicada. Na fase exploratória utiliza a produção científica de Ciência da Informação, especialmente nos trabalhos divulgados no Encontro Nacional de Pesquisa e Pós graduação em Ciência da Informação para identificar tendências de pesquisa sobre Competência em Informação e possíveis relações com a inclusão. Discute estas tendências relacionando-as com a tridimensionalidade que o termo inclusão propõe, a saber, inclusão social, inclusão digital e inclusão relacionada a pessoas com deficiência. Realiza a aplicação de pré testes (denominados de Pré Testes Indiciais) em situações de formação de professores. Adicionalmente analisa 18 modelos de Competência em Informação desenvolvidos em contextos internacionais cuja síntese permitiu propor grade básica para elaboração de um modelo de Competência em Informação. Na fase aplicada admite a influência teórica de conceitos de mediação como norteador da prática. Adicionalmente utiliza como método de abordagem a problematização e como técnica o desenvolvimento de um curso de formação de professores com 22 professores de uma escola púbica do Distrito Federal. Utilizou recursos didáticos originais elaborados para esta pesquisa, combinados com procedimentos de utilização e avaliação do modelo pedagógico para formação de professores em Competência em Informação numa perspectiva inclusiva. Na fase aplicada analisa a utilização deste modelo nas atividades, objetivos e habilidades propostas nas três etapas em que se estrutura, nomeadamente, pré-busca, busca propriamente dita e pós-busca. As atividades foram desenvolvidas e analisadas principalmente nas duas etapas do modelo- pré-busca e busca. Atividades de pós-busca não foram realizadas, visto que exigiriam abordagem longitudinal. Os resultados das atividades mostraram que a proposta pedagógica desenvolvida e aplicada é útil para a formação de professores de Educação Básica, em Competência em Informação numa perspectiva inclusiva.This research discusses the challenges of the information society regarding the promotion and assurance of an inclusive education based on an agenda established in supranational, international and multilateral environments. It highlights the need for teacher training to deal with informational needs emerging from educational practice, especially when applied to people with disabilities. In this context, it focuses on the interdisciplinary aspects between Information Science and Education contributions related to the inclusion and also the performance of Basic Education teachers. It admits the theoretical and practical assumptions of the theme Information Competency - a term adopted to the Information Literacy concept - as an alternative to Basic Education teachers’ training from the perspective of an inclusive education. It offers the use of a methodological path based on the contributions from studies on the Ginzburg Evidential Paradigm which is composed by an exploratory phase and an applied phase. In the exploratory phase it uses the scientific production of Information Science, especially in the works published in the National Meeting of Research and Postgraduate in Information Science to identify research trends about Information Competency and its possible relation to inclusion. It discusses those trends by relating them to the tridimensionality proposed by the term inclusion, namely, social inclusion, digital inclusion and inclusion related to people with disabilities. It performs the application of pre-tests (called Evidential Pre-tests) in situations of teacher training. Additionally it analyzes 18 models of Information Competency developed in international contexts which synthesis allowed proposing a basic grade to elaborate an Information Competency model. In the applied phase, it admits the theoretical influence of mediation concepts as a guide to the practice. Additionally it uses the problematization as an approach method and as technique the development of a teacher training course with 22 teachers from a public school in the Federal District. It used original didactic resources elaborated for this research combined with use and evaluation procedures of the pedagogical model for teachers’ training in Information Competency through an inclusive perspective. In the applied phase, it analyzes the use of this model on the activities, goals and abilities proposed through the three stages in which it is structured, namely pre-search, search itself and post-search. The activities were developed and analyzed mainly in the model stages of pre-search and search. Post-search activities were not performed, as they would require a longitudinal approach. The results of the activities showed that the pedagogical proposal developed and applied is useful to Basic Education teachers’ training in Information Competency through an inclusive perspective

A Case of Hepatocellular Carcinoma with Pulmonary Metastases Treated Successfully with a Combination of Repeated Hepatic Arterial Infusion Epirubicin and Cisplatin Chemotherapy and Systemic Low-Dose Infusion of 5-Fluorouracil

We report a case of hepatocellular carcinoma (HCC) with pulmonary metastases treated with repeated hepatic arterial infusion chemotherapy (HAIC) comprising epirubicin and cisplatin, and systemic infusion of 5-fluorouracil (a modified EC/F protocol), which led to complete remission. A 49-year-old man with compensated liver cirrhosis experienced intrahepatic recurrence of HCC with extensive lung metastases. The modified EC/F therapeutic protocol, which was applied at the tenth cycle every 4-5 weeks, resulted in disappearance of the pulmonary metastases and normalization of serum α-fetoprotein levels. A single small HCC lesion was found in the left lobe of the liver 13 months after the final chemotherapy session. HAIC with the same regimen was conducted again, followed by percutaneous intratumoral chemoinjection therapy with 5-fluorouracil and interferon-γ. Thereafter, there was no evidence of recurrence in either the liver or the lung, as evidenced by image analysis and expression of tumor markers. The disease-free intervals for the liver and lung were 41 and 54 months, respectively