Pojetí vyučovacího klimatu a faktory, které se na tvorbě vyučovacího klimatu podílejí

V příspěvku představujeme pojetí vyučovacího klimatu. Vymezujeme pojem faktor. Za faktory považujeme vzájemné vztahy mezi studenty ve třídě, pohotovost k výkonu, kooperaci, podporuučení a koncentrace na učení. Zabýváme se projevy těchto faktorů vyučovacího klimatu

Některé další faktory podílející se na tvorbě vyučovacího klimatu

V příspěvku se zabýváme dalšími faktory, které se podílejí na tvorbě vyučovacího klimatu. Pozorujeme a popisujeme projevy těchto faktorů: pořádek a organizovanost vyučování, jasnost pravidel, posuzování a hodnocení studentů, rozmanitost a participace ve vyučování, učitelovo nadšení a rozhled.In this raport we deal with other factors, that partake in formation of study climate. We study and describe the signs of these factors - order and orderliness of teaching, distinctness of rules, judging and assessment of students, multiplicity and participation in teaching, enthusiasm and insight of the teacher

In-vitro hemocompatibility of polyaniline functionalized by bioactive molecules

Hemocompatibility is an essential prerequisite for the application of materials in the field of biomedicine and biosensing. In addition, mixed ionic and electronic conductivity of conducting polymers is an advantageous property for these applications. Heparin-like materials containing sulfate, sulfamic, and carboxylic groups may have an anticoagulation effect. Therefore, sodium dodecylbenzenesulfonate, 2-aminoethane-1-sulfonic acid andN-(2-acetamido)-2-aminoethanesulfonic acid were used for modification of the representative of conducting polymers, polyaniline, and the resulting products were studied in the context of interactions with human blood. The anticoagulation activity was then correlated to surface energy and conductivity of the materials. Results show that anticoagulation activity is highly affected by the presence of suitable functional groups originating from the used heparin-like substances, and by the properties of polyaniline polymer itself. © 2019 by the authors.Czech Science FoundationGrant Agency of the Czech Republic [19-16861S]; Ministry of Education, Youth and Sports of the Czech Republic (NPU I) [LO1504]; TBU in Zlin [IGA/CPS/2019/004]; Slovenian Research AgencySlovenian Research Agency - Slovenia [P2-0082

Blood coagulation and platelet adhesion on polyaniline films

Polyaniline is a promising conducting polymer with still increasing application potential in biomedicine. Its surface modification can be an efficient way how to introduce desired functional groups and to control its properties while keeping the bulk characteristics of the material unchanged. The purpose of the study was to synthetize thin films of pristine conducting polyaniline hydrochloride, non-conducting polyaniline base and polyaniline modified with poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPSA) and investigate chosen parameters of their hemocompatibility. The modification was performed either by introduction of PAMPSA during the synthesis or by reprotonation of polyaniline base. The polyaniline hydrochloride and polyaniline base had no impact on blood coagulation and platelet adhesion. By contrast, the polyaniline reprotonated with PAMPSA completely hindered coagulation thanks to its interaction with coagulation factors Xa, Va and IIa. The significantly lower platelets adhesion was also found on this surface. Moreover, this film maintains its conductivity at pH of 6, which is an improvement in comparison with standard polyaniline hydrochloride losing most of its conductivity at pH of 4. Polyaniline film with PAMPSA introduced during synthesis had an impact on platelet adhesion but not on coagulation. The combined conductivity, anticoagulation activity, low platelet adhesion and improved conductivity at pH closer to physiological, open up new possibilities for application of polyaniline reprotonated by PAMPSA in blood-contacting devices, such as catheters or blood vessel grafts. © 2015 Elsevier B.V..Czech Science Foundation [13-08944S]; Ministry of Education, Youth and Sport of the Czech Republic [CZ.1.05/2.1.00/03.0111]; TBU in Zlin [IGA/FT/2014/004

Behaviour of titanium dioxide particles in artificial body fluids and human blood plasma

The growing application of materials containing TiO2 particles has led to an increased risk of human exposure, while a gap in knowledge about the possible adverse effects of TiO2 still exists. In this work, TiO2 particles of rutile, anatase, and their commercial mixture were exposed to various environments, including simulated gastric fluids and human blood plasma (both representing in vivo conditions), and media used in in vitro experiments. Simulated body fluids of different com-positions, ionic strengths, and pH were used, and the impact of the absence or presence of chosen enzymes was investigated. The physicochemical properties and agglomeration of TiO2 in these media were determined. The time dependent agglomeration of TiO2 related to the type of TiO2, and mainly to the type and composition of the environment that was observed. The presence of enzymes either prevented or promoted TiO2 agglomeration. TiO2 was also observed to exhibit concentration-dependent cytotoxicity. This knowledge about TiO2 behavior in all the abovementioned environments is critical when TiO2 safety is considered, especially with respect to the significant impact of the presence of proteins and size‐related cytotoxicity. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Czech Science FoundationGrant Agency of the Czech Republic [20-28732S]; Ministry of Education, Youth and Sports of the Czech Republic-grant DKRVO [RP/CPS/2020/001]; TBU in Zlin [IGA/CPS/2021/001]Ministerstvo Školství, Mládeže a Tělovýchovy, MŠMT: IGA/CPS/2021/001, RP/CPS/2020/001; Grantová Agentura České Republiky, GA ČR: 28732

Mechanism of the formation of DNA–protein cross-links by antitumor cisplatin

DNA–protein cross-links are formed by various DNA-damaging agents including antitumor platinum drugs. The natures of these ternary DNA–Pt–protein complexes (DPCLs) can be inferred, yet much remains to be learned about their structures and mechanisms of formation. We investigated the origin of these DPCLs and their cellular processing on molecular level using gel electrophoresis shift assay. We show that in cell-free media cisplatin [cis-diamminedichloridoplatinum(II)] forms DPCLs more effectively than ineffective transplatin [trans-diamminedichloridoplatinum(II)]. Mechanisms of transformation of individual types of plain DNA adducts of the platinum complexes into the DPCLs in the presence of several DNA-binding proteins have been also investigated. The DPCLs are formed by the transformation of DNA monofunctional and intrastrand cross-links of cisplatin. In contrast, interstrand cross-links of cisplatin and monofunctional adducts of transplatin are stable in presence of the proteins. The DPCLs formed by cisplatin inhibit DNA polymerization or removal of these ternary lesions from DNA by nucleotide excision repair system more effectively than plain DNA intrastrand or monofunctional adducts. Thus, the bulky DNA–protein cross-links formed by cisplatin represent a more distinct and persisting structural motif recognized by the components of downstream cellular systems processing DNA damage considerably differently than the plain DNA adducts of this metallodrug

Usage of business support for small and medium-sized firms

Import 10/08/2007Prezenční152 - Katedra podnikohospodářsk

Feasibility Study of Company

Import 01/09/2009Prezenční152 - Katedra podnikohospodářskávýborn

Školní výkonnost a sociálně-psychologické otázky žáků vycházejících ze zvláštní školy a z nižších než osmých ročníků základní devítileté školy

Katedra psychologieFilozofická fakult