Male apoE*3-Leiden.CETP mice on high-fat high-cholesterol diet exhibit a biphasic dyslipidemic response, mimicking the changes in plasma lipids observed through life in men

Physiological adaptations resulting in the development of the metabolic syndrome in man occur over a time span of several decades. This combined with the prohibitive financial cost and ethical concerns to measure key metabolic parameters repeatedly in subjects for the major part of their life span makes that comprehensive longitudinal human data sets are virtually nonexistent. While experimental mice are often used, little is known whether this species is in fact an adequate model to better understand the mechanisms that drive the metabolic syndrome in man. We took up the challenge to study the response of male apoE*3-Leiden.CETP mice (with a humanized lipid profile) to a high-fat high-cholesterol diet for 6 months. Study parameters include body weight, food intake, plasma and liver lipids, hepatic transcriptome, VLDL - triglyceride production and importantly the use of stable isotopes to measure hepatic de novo lipogenesis, gluconeogenesis, and biliary/fecal sterol secretion to assess metabolic fluxes. The key observations include (1) high inter-individual variation; (2) a largely unaffected hepatic transcriptome at 2, 3, and 6 months; (3) a biphasic response curve of the main metabolic features over time; and (4) maximum insulin resistance preceding dyslipidemia. The biphasic response in plasma triglyceride and total cholesterol appears to mimic that of men in cross-sectional studies. Combined, these observations suggest that studies such as these can help to delineate the causes of metabolic derangements in patients suffering from metabolic syndrome

Imminent brain death: point of departure for potential heart-beating organ donor recognition

Contains fulltext : 88186.pdf (publisher's version ) (Closed access)PURPOSE: There is, in European countries that conduct medical chart review of intensive care unit (ICU) deaths, no consensus on uniform criteria for defining a potential organ donor. Although the term is increasingly being used in recent literature, it is seldom defined in detail. We searched for criteria for determination of imminent brain death, which can be seen as a precursor for organ donation. METHODS: We organized meetings with representatives from the field of clinical neurology, neurotraumatology, intensive care medicine, transplantation medicine, clinical intensive care ethics, and organ procurement management. During these meetings, all possible criteria were discussed to identify a patient with a reasonable probability to become brain dead (imminent brain death). We focused on the practical usefulness of two validated coma scales (Glasgow Coma Scale and the FOUR Score), brain stem reflexes and respiration to define imminent brain death. Further we discussed criteria to determine irreversibility and futility in acute neurological conditions. RESULTS: A patient who fulfills the definition of imminent brain death is a mechanically ventilated deeply comatose patient, admitted to an ICU, with irreversible catastrophic brain damage of known origin. A condition of imminent brain death requires either a Glasgow Coma Score of 3 and the progressive absence of at least three out of six brain stem reflexes or a FOUR score of E(0)M(0)B(0)R(0). CONCLUSION: The definition of imminent brain death can be used as a point of departure for potential heart-beating organ donor recognition on the intensive care unit or retrospective medical chart analysis.1 september 201

Reisjournaal van Paulus Gevers naar Batavia en Bantam, 1776-1777

The Rotterdam Patriot Paulus Gevers (1741-1797), sets sail on the 8th of December 1776 on the ship of Captain Adrianus Frederik de Graaff, 'De Jonge Lieve', as junior merchant of the 'Kamer Amsterdam' of the Dutch East India Company. He gives an almost daily account of life on board of the ship. On the 30th of March they reach the Cape of Good Hope. On the 14th of April they leave the Cape and arrive in Batavia on the 18th of June 1777. Paulus Gevers is a member of a delegation going to Bantam for the coronation of the new Sultan. He also gives an account of this journey and of the visit (August 22nd – September 14th 1777)

Diary of Paulus Gevers of his journey to Batavia and Bantam, 1776-1777

The Rotterdam Patriot Paulus Gevers (1741-1797), sets sail on the 8th of December 1776 on the ship of Captain Adrianus Frederik de Graaff, 'De Jonge Lieve', as junior merchant of the 'Kamer Amsterdam' of the Dutch East India Company. He gives an almost daily account of life on board of the ship. On the 30th of March they reach the Cape of Good Hope. On the 14th of April they leave the Cape and arrive in Batavia on the 18th of June 1777. Paulus Gevers is a member of a delegation going to Bantam for the coronation of the new Sultan. He also gives an account of this journey and of the visit (August 22nd – September 14th 1777)

Data underlying: Stacking domain morphology in epitaxial graphene on silicon carbide

 This dataset contains stitched AC-LEEM overviews visualizing stacking domain boundaries in three different, high-quality graphene on SiC samples, grown in three different manners. The samples exhibit domain boundaries with different morphology, as explored in the corresponding paper. More details on the files can be found in the README.</p

Bybel der natuure, door Jan Swammerdam, Amsteldammer. Of Historie der insecten, tot zeekere zoorten gebracht: door voorbeelden, ontleedkundige onderzoekingen van veelerhande kleine gediertens, als ook door kunstige kopere plaaten opgeheldert : Verrykt met ontelbaare waarnemingen van nooit ontdekte zeldzaamhedenin de natuur /

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How information on sexism may increase women’s perceptions of being excluded, threaten fundamental needs, and lower career motivation

The current research investigates the effects of exposure to information about the prevalence of sexism in society on women’s perceptions, needs, expectations, and career motivation. We examined this in four studies. The publication package contains four datasets in SPSS with syntaxes