Dzieci urodzone z zarodków przechowywanych w stanie zamrożenia przez 10 lat. Analiza 5 przypadków

Faced with a scientific and legal debate on human embryo cryopreservation in Poland we show 5 documented clinical cases of successful thawing and transfer of embryos cryopreserved for a long period of time (8-11 years), resulting in successful delivery by the biological or the recipient mother. Cases described include patients with different infertility diagnoses, subjected to different hormonal stimulation treatments. Different oocyte fertilization methods were performed, and the obtained embryos were frozen after 2, 3 or 4 days of in vitro culture using methods employing various cryoprotective agents and freezing curves. As a result of performed thawing and transfer procedures normal, healthy babies were born. Our results are consistent with the international reports on successful long-term storage of embryos, (including the longest known period of over 19 years) resulting in no detectable reduction of the developmental potential after thawing. In light of data shown here, we do not see any medical or biological reasons for legally-regulated limitation of the period of frozen embryo storage. Moreover, if frozen, long-term stored embryos are not threatened by destruction band if prenatal adoption is a real, clinically documented option, we fail to see any reason for legal limitations of embryo cryopreservation in human infertility treatment.W kontekście naukowej i legislacyjnej debaty dotyczącej kriokonserwacji zarodków człowieka w Polsce przedstawiamy 5 udokumentowanych przypadków wieloletniego (przez okres 8-11 lat), skutecznego przechowywania zarodków zakończonego urodzeniem dzieci przez ich biologiczną matkę lub przez matkę adopcyjną. W opisanych przypadkach diagnozowano rożne przyczyny niepłodności. Zastosowano rożne metody hormonalnej stymulacji. Zamrażane były zarodki w rożnych stadiach rozwoju po hodowli in vitro trwającej 2, 3 lub 4 dni, przy udziale rożnych środków osłaniających. W efekcie przeprowadzonych kriotransferów urodziły się zdrowe, prawidłowo rozwinięte dzieci. Przedstawione dane potwierdzają wcześniejsze międzynarodowe doniesienia o możliwości wieloletniego przechowywania (najdłuższy znany przypadek to 19 lat) zamrożonych zarodków bez uszczerbku dla ich zdolności życiowych. W świetle tych danych nie znajdujemy biologicznych czy medycznych przesłanek do ustawowego ograniczania okresu przechowywania kriokonserwowanych zarodków. Tym samym, skoro zamrożone zarodki nie są skazane na biologiczną degradację a ich prenatalna adopcja jest absolutnie realną możliwością, nie widzimy podstaw do prawnego ograniczania stosowania metody kriokonserwacji w leczeniu niepłodności

Effect of Amino Acids and Effective Microorganisms on Meadow Silage Chemical Composition

The effects of growth stimulants consist of activating plant physiological processes, which positively affects vegetative and generative growth, increasing the yield and its quality. The aim of the experiment was to study the effect of microbial and amino acid products on the quality of grassland silage; their application in different combinations was an experimental factor. According to the results, the foliar application of the amino acid and microbial products had a statistically significant effect on the chemical composition of silage. In comparison to control, silage produced from plants treated with those growth stimulants contained significantly more total protein in dry matter, while crude fiber content changed only slightly. In addition, due to higher lactic acid content, the quality of silage treated with the stimulants was higher than the quality of control plants. To sum up, the foliar treatment of plants with microorganisms in combination with amino acids resulted in a significant increase in the content of desired components, with a higher nutritional value of the silage

ILC Reference Design Report Volume 1 - Executive Summary

The International Linear Collider (ILC) is a 200-500 GeV center-of-mass high-luminosity linear electron-positron collider, based on 1.3 GHz superconducting radio-frequency (SCRF) accelerating cavities. The ILC has a total footprint of about 31 km and is designed for a peak luminosity of 2x10^34 cm^-2s^-1. This report is the Executive Summary (Volume I) of the four volume Reference Design Report. It gives an overview of the physics at the ILC, the accelerator design and value estimate, the detector concepts, and the next steps towards project realization.The International Linear Collider (ILC) is a 200-500 GeV center-of-mass high-luminosity linear electron-positron collider, based on 1.3 GHz superconducting radio-frequency (SCRF) accelerating cavities. The ILC has a total footprint of about 31 km and is designed for a peak luminosity of 2x10^34 cm^-2s^-1. This report is the Executive Summary (Volume I) of the four volume Reference Design Report. It gives an overview of the physics at the ILC, the accelerator design and value estimate, the detector concepts, and the next steps towards project realization

ILC Reference Design Report Volume 4 - Detectors

This report, Volume IV of the International Linear Collider Reference Design Report, describes the detectors which will record and measure the charged and neutral particles produced in the ILC's high energy e+e- collisions. The physics of the ILC, and the environment of the machine-detector interface, pose new challenges for detector design. Several conceptual designs for the detector promise the needed performance, and ongoing detector R&D is addressing the outstanding technological issues. Two such detectors, operating in push-pull mode, perfectly instrument the ILC interaction region, and access the full potential of ILC physics.This report, Volume IV of the International Linear Collider Reference Design Report, describes the detectors which will record and measure the charged and neutral particles produced in the ILC's high energy e+e- collisions. The physics of the ILC, and the environment of the machine-detector interface, pose new challenges for detector design. Several conceptual designs for the detector promise the needed performance, and ongoing detector R&D is addressing the outstanding technological issues. Two such detectors, operating in push-pull mode, perfectly instrument the ILC interaction region, and access the full potential of ILC physics

ILC Reference Design Report Volume 3 - Accelerator

The International Linear Collider (ILC) is a 200-500 GeV center-of-mass high-luminosity linear electron-positron collider, based on 1.3 GHz superconducting radio-frequency (SCRF) accelerating cavities. The ILC has a total footprint of about 31 km and is designed for a peak luminosity of 2x10^34 cm^-2 s^-1. The complex includes a polarized electron source, an undulator-based positron source, two 6.7 km circumference damping rings, two-stage bunch compressors, two 11 km long main linacs and a 4.5 km long beam delivery system. This report is Volume III (Accelerator) of the four volume Reference Design Report, which describes the design and cost of the ILC.The International Linear Collider (ILC) is a 200-500 GeV center-of-mass high-luminosity linear electron-positron collider, based on 1.3 GHz superconducting radio-frequency (SCRF) accelerating cavities. The ILC has a total footprint of about 31 km and is designed for a peak luminosity of 2x10^34 cm^-2 s^-1. The complex includes a polarized electron source, an undulator-based positron source, two 6.7 km circumference damping rings, two-stage bunch compressors, two 11 km long main linacs and a 4.5 km long beam delivery system. This report is Volume III (Accelerator) of the four volume Reference Design Report, which describes the design and cost of the ILC

International Linear Collider Reference Design Report Volume 2: PHYSICS AT THE ILC

This article reviews the physics case for the ILC. Baseline running at 500 GeV as well as possible upgrades and options are discussed. The opportunities on Standard Model physics, Higgs physics, Supersymmetry and alternative theories beyond the Standard Model are described.This article reviews the physics case for the ILC. Baseline running at 500 GeV as well as possible upgrades and options are discussed. The opportunities on Standard Model physics, Higgs physics, Supersymmetry and alternative theories beyond the Standard Model are described