Discovery and analysis of p-mode and g-mode oscillations in the A-type primary of the eccentric binary HD 209295

We have discovered both intermediate-order gravity mode and low-order pressure mode pulsation in the same star, HD 209295. It is therefore both a γ Doradus and a δ Scuti star, which makes it the first pulsating star to be a member of two classes. The analysis of our 128 h of multisite spectroscopic observations carried out over two seasons reveals that the star is a single-lined spectroscopic binary with an orbital period of 3.10575±0.00010 d and an eccentricity of 0.352±0.011. Only weak pulsational signals are found in both the radial velocity and line-profile variations, but we have succeeded in showing that the two highest-amplitude γ Doradus pulsation modes are consistent with ℓ=1 and ∣m∣=1. These two modes dominated our 280 h of BVIC multisite photometry, also obtained over two seasons. We detected altogether ten frequencies in the light variations, one in the δ Scuti regime and nine in the γ Doradus domain. Five of the γ Doradus frequencies are exact integer multiples of the orbital frequency. This observation leads us to suspect they are tidally excited. Attempts to identify modes from the multicolour photometry failed. We performed model calculations and a stability analysis of the pulsations. The frequency range in which δ Scuti modes are excited agrees well with observations. However, our models do not show excitation of γ Doradus pulsations, although the damping is smaller in the observed range. We also investigated tidal excitation of γ Doradus modes. Some of the observed harmonics of the orbital period were found to be unstable. The observed orbital harmonics which are stable in the models can be understood as linear combinations of the unstable modes. We could not detect the secondary component of the system in infrared photometry, suggesting that it may not be a main-sequence star. Archival data of this star show that it has a strong ultraviolet (UV) excess, the origin of which is not known. The orbit of the primary is consistent with a secondary mass of M>1.04 M⊙, which is indicative of a neutron star, although a white dwarf companion is not ruled ou

Discovery and analysis of p-mode and g-mode oscillations in the A-type primary of the eccentric binary HD 209295

We have discovered both intermediate-order gravity mode and low-order pressure mode pulsation in the same star, HD 209295. It is therefore both a Gamma Doradus and a Delta Scuti star, which makes it the first pulsating star to be a member of two classes. The star is a single-lined spectroscopic binary with an orbital period of 3.10575 d and an eccentricity of 0.352. Weak pulsational signals are found in both the radial velocity and line-profile variations, allowing us to show that the two highest-amplitude Gamma Doradus pulsation modes are consistent with l=1 and |m|=1. In our 280 h of BVI multi-site photometry we detected ten frequencies in the light variations, one in the Delta Scuti regime and nine in the Gamma Doradus domain. Five of the Gamma Doradus frequencies are exact integer multiples of the orbital frequency. This observation leads us to suspect they are tidally excited. Results of theoretical modeling (stability analysis, tidal excitation) were consistent with the observations. We could not detect the secondary component of the system in infrared photometry, suggesting that it may not be a main-sequence star. Archival data of HD 209295 show a strong ultraviolet excess, the origin of which is not known. The orbit of the primary is consistent with a secondary mass of M > 1.04 Msun indicative of a neutron star or a white dwarf companion.Comment: 18 pages, 18 figures, accepted for publication in MNRAS, shortened abstrac

A root's ability to retain K+ correlates with salt tolerance in wheat

Most work on wheat breeding for salt tolerance has focused mainly on excluding Na+ from uptake and transport to the shoot. However, some recent findings have reported no apparent correlation between leaf Na+ content and wheat salt tolerance. Thus, it appears that excluding Na+ by itself is not always sufficient to increase plant salt tolerance and other physiological traits should also be considered. In this work, it was investigated whether a root's ability to retain K+ may be such a trait, and whether our previous findings for barley can be extrapolated to species following a ‘salt exclusion’ strategy. NaCl-induced kinetics of K+ flux from roots of two bread and two durum wheat genotypes, contrasting in their salt tolerance, were measured under laboratory conditions using non-invasive ion flux measuring (the MIFE) technique. These measurements were compared with whole-plant physiological characteristics and yield responses from plants grown under greenhouse conditions. The results show that K+ flux from the root surface of 6-d-old wheat seedlings in response to salt treatment was highly correlated with major plant physiological characteristics and yield of greenhouse-grown plants. This emphasizes the critical role of K+ homeostasis in plant salt tolerance and suggests that using NaCl-induced K+ flux measurements as a physiological ‘marker’ for salt tolerance may benefit wheat-breeding programmes

Treatment of chronically depressed patients: A multisite randomized controlled trial testing the effectiveness of \u27Cognitive Behavioral Analysis System of Psychotherapy\u27 (CBASP) for chronic depressions versus usual secondary care

Background \u27Cognitive Behavioral Analysis System of Psychotherapy\u27 (CBASP) is a form of psychotherapy specifically developed for patients with chronic depression. In a study in the U.S., remarkable favorable effects of CBASP have been demonstrated. However, no other studies have as yet replicated these findings and CBASP has not been tested outside the United States. This protocol describes a randomized controlled trial on the effectiveness of CBASP in the Netherlands. Methods/Design The purpose of the present paper is to report the study protocol of a multisite randomized controlled trial testing the effectiveness of \u27Cognitive Behavioral Analysis System of Psychotherapy\u27 (CBASP) for chronic depression in the Netherlands. In this study, CBASP in combination with medication, will be tested versus usual secondary care in combination with medication. The aim is to recruit 160 patients from three mental health care organizations. Depressive symptoms will be assessed at baseline, after 8 weeks, 16 weeks, 32 weeks and 52 weeks, using the 28-item Inventory for Depressive Symptomatology (IDS). Effect modification by co morbid anxiety, alcohol consumption, general and social functioning and working alliance will be tested. GEE analyses of covariance, controlling for baseline value and center will be used to estimate the overall treatment effectiveness (difference in IDS score) at post-treatment and follow up. The primary analysis will be by \u27intention to treat\u27 using double sided tests. An economic analysis will compare the two groups in terms of mean costs and cost-effectiveness from a societal perspective. Discussion The study will provide an answer to the question whether the favorable effects of CBASP can be replicated outside the US. Trial Registration The Dutch Cochrane Center, NTR1090

Preparing clinical-grade myeloid dendritic cells by electroporation-mediated transfection of in vitro amplified tumor-derived mRNA and safety testing in stage IV malignant melanoma

BACKGROUND: Dendritic cells (DCs) have been used as vaccines in clinical trials of immunotherapy of cancer and other diseases. Nonetheless, progress towards the use of DCs in the clinic has been slow due in part to the absence of standard methods for DC preparation and exposure to disease-associated antigens. Because different ex vivo exposure methods can affect DC phenotype and function differently, we studied whether electroporation-mediated transfection (electrotransfection) of myeloid DCs with in vitro expanded RNA isolated from tumor tissue might be feasible as a standard physical method in the preparation of clinical-grade DC vaccines. METHODS: We prepared immature DCs (IDCs) from CD14(+ )cells isolated from leukapheresis products and extracted total RNA from freshly resected melanoma tissue. We reversely transcribed the RNA while attaching a T7 promoter to the products that we subsequently amplified by PCR. We transcribed the amplified cDNA in vitro and introduced the expanded RNA into IDCs by electroporation followed by DC maturation and cryopreservation. Isolated and expanded mRNA was analyzed for the presence of melanoma-associated tumor antigens gp100, tyrosinase or MART1. To test product safety, we injected five million DCs subcutaneously at three-week intervals for up to four injections into six patients suffering from stage IV malignant melanoma. RESULTS: Three preparations contained all three transcripts, one isolate contained tyrosinase and gp100 and one contained none. Electrotransfection of DCs did not affect viability and phenotype of fresh mature DCs. However, post-thaw viability was lower (69 ± 12 percent) in comparison to non-electroporated cells (82 ± 12 percent; p = 0.001). No patient exhibited grade 3 or 4 toxicity upon DC injections. CONCLUSION: Standardized preparation of viable clinical-grade DCs transfected with tumor-derived and in vitro amplified mRNA is feasible and their administration is safe

Assessing the impact of the addition of pyriproxyfen on the durability of permethrin-treated bed nets in Burkina Faso: a compound-randomized controlled trial

Background Long-lasting insecticidal nets (LLINs) treated with pyrethroids are the foundation of malaria control in sub-Saharan Africa. Rising pyrethroid resistance in vectors, however, has driven the development of alternative net formulations. Here the durability of polyethylene nets with a novel combination of a pyrethroid, permethrin, and the insect juvenile hormone mimic, pyriproxyfen (PPF), compared to a standard permethrin LLIN, was assessed in rural Burkina Faso. Methods A compound-randomized controlled trial was completed in two villages. In one village 326 of the PPF-permethrin nets (Olyset Duo) and 327 standard LLINs (Olyset) were distributed to assess bioefficacy. In a second village, 170 PPF-permethrin nets and 376 LLINs were distributed to assess survivorship. Nets were followed at 6-monthly intervals for 3 years. Bioefficacy was assessed by exposing permethrin-susceptible and resistant Anopheles gambiae sensu lato mosquito strains to standard World Health Organization (WHO) cone and tunnel tests with impacts on fertility measured in the resistant strain. Insecticide content was measured using high-performance liquid chromatography. LLIN survivorship was recorded with a questionnaire and assessed by comparing the physical integrity using the proportionate hole index (pHI). Results The PPF-permethrin net met WHO bioefficacy criteria (≥ 80% mortality or ≥ 95% knockdown) for the first 18 months, compared to 6 months for the standard LLIN. Mean mosquito mortality for PPF-permethrin nets, across all time points, was 8.6% (CI 2.6–14.6%) higher than the standard LLIN. Fertility rates were reduced after PPF-permethrin net exposure at 1-month post distribution, but not later. Permethrin content of both types of nets remained within the target range of 20 g/kg ± 25% for 242/248 nets tested. The pyriproxyfen content of PPF-permethrin nets declined by 54%, from 10.4 g/kg (CI 10.2–10.6) to 4.7 g/kg (CI 3.5–6.0, p < 0.001) over 36 months. Net survivorship was poor, with only 13% of PPF-permethrin nets and 12% of LLINs still present in the original household after 36 months. There was no difference in the fabric integrity or survivorship between the two net types. Conclusion The PPF-permethrin net, Olyset Duo, met or exceeded the performance of the WHO-recommended standard LLIN (Olyset) in the current study but both net types failed the 3-year WHO bioefficacy criteria