DEVELOPMENT AND IN VITRO EVALUATION OF VALSARTAN-LOADED RESEALED ERYTHROCYTES

Objective: Resealed erythrocyte technique has attempts for preparation of valsartan loaded reservoir type using red blood cell as a carrier. Methods: The resealed erythrocytes were prepared by using glutaraldehyde as a cross-linking agent by Preswell dilution technique and sodium chloride as the medium has been made to predict mechanism of drug release and absorption based on the order of release. The prepared resealed erythrocytes was characterised as a percentage of cell recovery drug content, osmotic shock, turbulence fragility, osmotic fragility, in vitro drug release studies, and hemoglobin content study. Results: The result of the study showed that the resealed erythrocytes prepared with sodium chloride (9 %) showed biconcave shape, an assay of 6.5±0.4 %, osmotic shock (0.028±0.004 µg/ml), turbulence fragility of 0.228±0.046 µg/ml, and 44.38±5.54 % drug release in 8 h. The drug release kinetics was studied and found that release from spherical matrices, first-order model with non-fickian diffusion with and the dissolution occurs in planes that are parallel to the drug surface pattern. Conclusion: VaL administration could be avoided the number of drawbacks associated with systemic delivery and perhaps maintain a relatively constant plasma level during a lengthy course of treatment

A Nonlinear Analytical Model for Symmetric Laminated Beams in Three-point Bending

The use of composite materials with continuous fibers in the aircraft and aerospace industries requires a thorough knowledge of behaviors of these laminate composites under various loading conditions. Indeed, the aim of this work is to simulate linear and nonlinear behavior of a symmetric laminated composite under three-point bending tests. The modelization used is based on an analytical approximation that has been recently developed for isotropic materials. This approximation is still valid for the studied quasi-isotropic laminated composite because it is symmetric with a specific layer sequence. The overall response of laminate composite is determined from the behavior of each ply outside of their orthotropic axis. Two methods are used to calculate the equivalent longitudinal Young-modulus of the laminate. The result shows that when the deflection of the specimens is less than 2.5 times the thickness, the difference between the experimental and analytical curves is about 1% for the average global stresses method, and about 7.5% for the apparent bending modulus method. For large deflections, the difference relative to the first method remains less than 11% and the second method is about 20%

EBV Associated Breast Cancer Whole Methylome Analysis Reveals Viral and Developmental Enriched Pathways

Background: Breast cancer (BC) ranks among the most common cancers in Sudan and worldwide with hefty toll on female health and human resources. Recent studies have uncovered a common BC signature characterized by low frequency of oncogenic mutations and high frequency of epigenetic silencing of major BC tumor suppressor genes. Therefore, we conducted a pilot genome-wide methylome study to characterize aberrant DNA methylation in breast cancer.Results: Differential methylation analysis between primary tumor samples and normal samples from healthy adjacent tissues yielded 20,188 differentially methylated positions (DMPs), which is further divided into 13,633 hypermethylated sites corresponding to 5339 genes and 6,555 hypomethylated sites corresponding to 2811 genes. Moreover, bioinformatics analysis revealed epigenetic dysregulation of major developmental pathways including hippo signaling pathway. We also uncovered many clues to a possible role for EBV infection in BC.Conclusion: Our results clearly show the utility of epigenetic assays in interrogating breast cancer tumorigenesis, and pinpointing specific developmental and viral pathways dysregulation that might serve as potential biomarkers or targets for therapeutic interventions

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Processing and decisions relating to water resources data: The case of Morocco

The national water strategy has been an essential vector of government strategy for a long time. The management of water resources is an integral part of the economic development of Morocco. Nevertheless, the definition of the strategic axes of this component and the adequate decision-making depends directly on the collection and use of all the data relating to water resources. If big data technologies present a suitable solution to ensure optimal and rapid use of its data, the success of functional and technical designs can only be provided after total control of the processing and decision-making processes relating to the water domain. In this paper, we will try to identify the aspects relating to the processes of data collection, processing, consolidation, and decision-making through the use of the results of field surveys and interviews with business managers

STUDIES ON POLY HERBAL POWDER SHAMPOO FOR THE TREATMENT OF PEDICULOSIS CAPITIS AND PITYRIASIS CAPITIS INFESTATIONS

Objective: The main objective of this study was to formulation containing poly herbal medicinal plants, which gives safety, efficacy; rectify the skin disease, scalp disorders and reducing harmful effect from herbal anti-lice and dandruff shampoos for the treatment of Pediculosis capitis and Pityriasis capitis and to prevent hair fall. Methods: The formulation of herbal shampoo with various herbal ingredients were dried, milled, sieved (120#) and mixed in geometrical order and evaluated their physicochemical characteristics. Furthermore, particle characters, organoleptic properties, foaming index, skin irritation study, and Anti-lice activity were performed.  Results: The prepared poly herbal powdered shampoo (PHPS) exhibits that was freely soluble, particle 20-25 μm size range and free flowing powder. Lest moisture content (2.5%), ash value, acid value and reduced surface tension of liquid referred as an ideal candidate for foaming, anti-lice potential with no skin irritations. Conclusion: Based on the evaluation parameters, the prepared powdered shampoo helps to remove hair grease, no hair fall and strengthen hair follicles. Preparation of PHPS formulation at laboratory scale and the compounding of several ingredients of herbal source have produced it possible to ensure safety, efficacy and secure highly effective PHPS

Flood hazards in an urbanizing watershed in Riyadh, Saudi Arabia

Riyadh, the capital of the Kingdom of Saudi Arabia, has experienced unusual levels of urbanization in the past few decades, making it one of the fastest growing cities in the world. This paper examines flood hazards in the rapidly urbanizing catchment of Al-Aysen in Riyadh. Remote sensing and geographic information system techniques were employed to obtain and prepare input data for hydrologic and hydraulic models, with the former based on the very popular curve number approach. Due to the limited nature of the rainfall data, observations from two rain gauges in the vicinity of the catchment were used to estimate design storms. The hydrologic model was run in a semi-distributed mode by dividing the catchment into many sub-catchments. The impact of urbanization on run-off volume and peak discharge resulting from different storms was investigated, with various urbanization scenarios simulated. Flood hazard zones and affected streets were also identified through hydrologic/hydraulic model simulation. The mismatch between administrative and catchment boundaries can create problems in flood risk management for similar cities since hydrologic processes and flood hazards are based on the hydrologic connectivity. Since flooding events impact the road network and create driving hazards, governmental decision-makers must take the necessary precautions to protect drivers in these situations

Pancreatic surgery outcomes: multicentre prospective snapshot study in 67 countries

Background: Pancreatic surgery remains associated with high morbidity rates. Although postoperative mortality appears to have improved with specialization, the outcomes reported in the literature reflect the activity of highly specialized centres. The aim of this study was to evaluate the outcomes following pancreatic surgery worldwide.Methods: This was an international, prospective, multicentre, cross-sectional snapshot study of consecutive patients undergoing pancreatic operations worldwide in a 3-month interval in 2021. The primary outcome was postoperative mortality within 90 days of surgery. Multivariable logistic regression was used to explore relationships with Human Development Index (HDI) and other parameters.Results: A total of 4223 patients from 67 countries were analysed. A complication of any severity was detected in 68.7 percent of patients (2901 of 4223). Major complication rates (Clavien-Dindo grade at least IIIa) were 24, 18, and 27 percent, and mortality rates were 10, 5, and 5 per cent in low-to-middle-, high-, and very high-HDI countries respectively. The 90-day postoperative mortality rate was 5.4 per cent (229 of 4223) overall, but was significantly higher in the low-to-middle-HDI group (adjusted OR 2.88, 95 per cent c.i. 1.80 to 4.48). The overall failure-to-rescue rate was 21 percent; however, it was 41 per cent in low-to-middle-compared with 19 per cent in very high-HDI countries.Conclusion: Excess mortality in low-to-middle-HDI countries could be attributable to failure to rescue of patients from severe complications. The authors call for a collaborative response from international and regional associations of pancreatic surgeons to address management related to death from postoperative complications to tackle the global disparities in the outcomes of pancreatic surgery (NCT04652271; ISRCTN95140761)