Prokaryotic and eukaryotic skin microbiota modifications triggered by Leishmania infection in localized Cutaneous Leishmaniasis.

Cutaneous Leishmaniasis (CL) is a tropical disease characterized by cutaneous ulcers, sometimes with satellite lesions and nodular lymphangitis. Leishmania parasites, transmitted by sandfly vectors, cause this widespread public health challenge affecting millions worldwide. CL's complexity stems from diverse Leishmania species and intricate host interactions. Therefore, this study aims to shed light on the spatial-temporal distribution of Leishmania species and exploring the influence of skin microbiota on disease progression. We analyzed 40 samples from CL patients at three military bases across Colombia. Using Oxford Nanopore's Heat Shock Protein 70 sequencing, we identified Leishmania species and profiled microbiota in CL lesions and corresponding healthy limbs. Illumina sequencing of 16S-rRNA and 18S-rRNA genes helped analyze prokaryotic and eukaryotic communities. Our research uncovered a spatial-temporal overlap between regions of high CL incidence and our sampling locations, indicating the coexistence of various Leishmania species. L. naiffi emerged as a noteworthy discovery. In addition, our study delved into the changes in skin microbiota associated with CL lesions sampled by scraping compared with healthy skin sampled by brushing of upper and lower limbs. We observed alterations in microbial diversity, both in prokaryotic and eukaryotic communities, within the lesioned areas, signifying the potential role of microbiota in CL pathogenesis. The significant increase in specific bacterial families, such as Staphylococcaceae and Streptococcaceae, within CL lesions indicates their contribution to local inflammation. In essence, our study contributes to the ongoing research into CL, highlighting the need for a multifaceted approach to decipher the intricate interactions between Leishmaniasis and the skin microbiota

Body mass index and disease activity in chronic inflammatory rheumatic diseases: results of the Cardiovascular in Rheumatology (Carma) Project

Objective: Since obesity has been associated with a higher inflammatory burden and worse response to therapy in patients with chronic inflammatory rheumatic diseases (CIRD), we aimed to confirm the potential association between body mass index (BMI) and disease activity in a large series of patients with CIRDs included in the Spanish CARdiovascular in rheuMAtology (CARMA) registry. Methods: Baseline data analysis of patients included from the CARMA project, a 10-year prospective study of patients with rheumatoid arthritis (RA), ankylosing spondylitis (AS), and psoriatic arthritis (PsA) attending outpatient rheumatology clinics from 67 Spanish hospitals. Obesity was defined when BMI (kg/m2) was >30 according to the WHO criteria. Scores used to evaluate disease activity were Disease Activity Score of 28 joints (DAS28) in RA, Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) in AS, and modified DAS for PsA. Results: Data from 2234 patients (775 RA, 738 AS, and 721 PsA) were assessed. The mean ± SD BMI at the baseline visit were: 26.9 ± 4.8 in RA, 27.4 ± 4.4 in AS, and 28.2 ± 4.7 in PsA. A positive association between BMI and disease activity in patients with RA (β = 0.029; 95%CI (0.01- 0.05); p = 0.007) and PsA (β = 0.036; 95%CI (0.015-0.058); p = 0.001) but not in those with AS (β = 0.001; 95%CI (-0.03-0.03); p = 0.926) was found. Disease activity was associated with female sex and rheumatoid factor in RA and with Psoriasis Area Severity Index and enthesitis in PsA. Conclusions: BMI is associated with disease activity in RA and PsA, but not in AS. Given that obesity is a potentially modifiable factor, adequate control of body weight can improve the outcome of patients with CIRD and, therefore, weight control should be included in the management strategy of these patients

Ciencia en Sociedad. Reflexiones en el marco de su relación bidireccional

Con autorización de la editorial para este libro. La edición de este libro estuvo a cargo de Jesús Rey Rocha y Víctor Ladero.Este libro surge como una iniciativa para compilar una serie de artículos alumbrados a partir de la génesis del Grupo CURIE (Científic@s Unid@s por la Reactivación de la Investigación en España), semilla de la Asociación Española para el Avance de la Ciencia (AEAC). Durante meses sus miembros estuvieron intercambiando reflexiones y debatiendo sobre la distancia entre la ciencia y la sociedad, y la necesidad de crear una asociación que llenase ese vacío entre los científicos y los ciudadanos, que apostase por llevar la ciencia y los conocimientos generados por la misma a toda la sociedad, tal y como establecen la Declaración de Derechos Humanos y la Constitución Española, en su artículo 44.Peer reviewe

Investigar sin parar, la consigna de Jesús Jaimes para ser un profesional exitoso

Jesús Jaimes –un estudiante rosarista que hace la doble titulación en Medicina y Biología– tenía claro desde temprano que su vida profesional se encontraba en la investigación y la ciencia.Jesús Jaimes –a Rosarista student doing a double degree in Medicine and Biology– was clear from an early age that his professional life was based on research and science

Investigar sin parar, la consigna de Jesús Jaimes para ser un profesional exitoso

Jesús Jaimes –un estudiante rosarista que hace la doble titulación en Medicina y Biología– tenía claro desde temprano que su vida profesional se encontraba en la investigación y la ciencia.Jesús Jaimes –a Rosarista student doing a double degree in Medicine and Biology– was clear from an early age that his professional life was based on research and science

Dressler’s syndrome : the return of a forgotten entity

El síndrome de Dressler es una enfermedad infrecuente que fue descrita por primera vez en 1956 por el doctor William Dressler. Corresponde a un cuadro de pericarditis secundaria, en ocasiones asociada a efusión pericárdica de aparente etiología autoinmunitaria, que hace parte de los síndromes de lesión poscardiaca. Se presenta un caso de síndrome de Dressler en un paciente joven.Q4Dressler’s syndrome is a rare entity which was first described in 1956 by Dr. William Dressler. It consists of secondary peri carditis, at times associated with pericardial effusion with an apparently autoimmune etiology, which is one of the post-cardiac injury syndromes. We present a case of Dressler’s syndrome in a young patient.Revista Nacional - IndexadaS

Learn by doing as a didactic strategy through the codecademy web portal in pedagogical practice to promote creative thinking and significant learning in students

The work developed is action research type, framed in the qualitative paradigm. It designs a didactic strategy for the development of a different pedagogical practice to the traditional one that allows a greater autonomy in the student during the learning process, since the apprehension of knowledge is done in a more personal and practical way. The didactic proposal is developed from the diagnosis of the student's creativity and their ability to interact with academic platforms. Its implementation allows the student to learn by doing in a process of trial, error and correction in order to promote creative thinking while achieving meaningful learning

Identification and Real Time Speed Control of a Series DC Motor

The identification and real time speed control, without reverse motion, for a series DC motor is presented. The identification is performed using the transient response analysis of the mechanical and electrical subsystems of a series DC motor. A linearized model which does not include the magnetic saturation around the operating conditions is considered. Based on this model, a PI speed controller is designed. A well-known problem arising in this type of electrical motors is the singularity at zero speed. It is shown that, in spite of this inconvenience, the PI controller, together with an antiwindup scheme, presents adequate regulation and tracking performance. It is also shown that the control system can compensate for varying loads and the counter-electromotive force with acceptable levels of current consumption

Beta diversity indices of prokaryotes and eukaryotes by <i>Leishmania</i> species.

Beta diversity of prokaryotes (A) and eukaryotes (B) based on the sample type (healthy skin (Healthy) or the lesion (Lesion)) divided by Leishmania species. There are not significant differences between species groups. (TIF)</p

Differential abundance analysis of prokaryotic phyla using the DESeq2 package.

Denoting uncovered an increase in abundance for the Firmicutes and Proteobacteria phyla, owing to positive rate changes. (TIF)</p