Shotgun Phage Display - Selection for Bacterial Receptins or other Exported Proteins

Shotgun phage display cloning involves construction of libraries from randomly fragmented bacterial chromosomal DNA, cloned genes, or eukaryotic cDNAs, into a phagemid vector. The library obtained consists of phages expressing polypeptides corresponding to all genes encoded by the organism, or overlapping peptides derived from the cloned gene. From such a library, polypeptides with affinity for another molecule can be isolated by affinity selection, panning. The technique can be used to identify bacterial receptins and identification of their minimal binding domain, and but also to identify epitopes recognised by antibodies. In addition, after modification of the phagemid vector, the technique has also been used to identify bacterial extracytoplasmic proteins

Design of a high power production target for the Beam Dump Facility at CERN

The Beam Dump Facility (BDF) project is a proposed general-purpose facility at CERN, dedicated to beam dump and fixed target experiments. In its initial phase, the facility is foreseen to be exploited by the Search for Hidden Particles (SHiP) experiment. Physics requirements call for a pulsed 400 GeV/c proton beam as well as the highest possible number of protons on target (POT) each year of operation, in order to search for feebly interacting particles. The target/dump assembly lies at the heart of the facility, with the aim of safely absorbing the full high intensity Super Proton Synchrotron (SPS) beam, while maximizing the production of charmed and beauty mesons. High-Z materials are required for the target/dump, in order to have the shortest possible absorber and reduce muon background for the downstream experiment. The high average power deposited on target (305 kW) creates a challenge for heat removal. During the BDF facility Comprehensive Design Study (CDS), launched by CERN in 2016, extensive studies have been carried out in order to define and assess the target assembly design. These studies are described in the present contribution, which details the proposed design of the BDF production target, as well as the material selection process and the optimization of the target configuration and beam dilution. One of the specific challenges and novelty of this work is the need to consider new target materials, such as a molybdenum alloy (TZM) as core absorbing material and Ta2.5W as cladding. Thermo-structural and fluid dynamics calculations have been performed to evaluate the reliability of the target and its cooling system under beam operation. In the framework of the target comprehensive design, a preliminary mechanical design of the full target assembly has also been carried out, assessing the feasibility of the whole target system.Comment: 17 pages, 18 figure

Introduction to Nordic Mediation Research

Non peer reviewe

BDNF polymorphisms are linked to poorer working memory performance, reduced cerebellar and hippocampal volumes and differences in prefrontal cortex in a Swedish elderly population

BACKGROUND: Brain-derived neurotrophic factor (BDNF) links learning, memory and cognitive decline in elderly, but evidence linking BDNF allele variation, cognition and brain structural differences is lacking. METHODS: 367 elderly Swedish men (n = 181) and women (n = 186) from Prospective Investigation of the Vasculature in Uppsala seniors (PIVUS) were genotyped and the BDNF functional rs6265 SNP was further examined in subjects who completed the Trail Making Task (TMT), verbal fluency task, and had a magnetic resonance imaging (MRI) scan. Voxel-based morphometry (VBM) examined brain structure, cognition and links with BDNF. RESULTS: The functional BDNF SNP (rs6265,) predicted better working memory performance on the TMT with positive association of the Met rs6265, and was linked with greater cerebellar, precuneus, left superior frontal gyrus and bilateral hippocampal volume, and reduced brainstem and bilateral posterior cingulate volumes. CONCLUSIONS: The functional BDNF polymorphism influences brain volume in regions associated with memory and regulation of sensorimotor control, with the Met rs6265 allele potentially being more beneficial to these functions in the elderly

The role of matter density uncertainties in the analysis of future neutrino factory experiments

Matter density uncertainties can affect the measurements of the neutrino oscillation parameters at future neutrino factory experiments, such as the measurements of the mixing parameters $\theta_{13}$ and \deltacp. We compare different matter density uncertainty models and discuss the possibility to include the matter density uncertainties in a complete statistical analysis. Furthermore, we systematically study in which measurements and where in the parameter space matter density uncertainties are most relevant. We illustrate this discussion with examples that show the effects as functions of different magnitudes of the matter density uncertainties. We find that matter density uncertainties are especially relevant for large \stheta \gtrsim 10^{-3}. Within the KamLAND-allowed range, they are most relevant for the precision measurements of \stheta and \deltacp, but less relevant for ``binary'' measurements, such as for the sign of \ldm, the sensitivity to \stheta, or the sensitivity to maximal CP violation. In addition, we demonstrate that knowing the matter density along a specific baseline better than to about 1% precision means that all measurements will become almost independent of the matter density uncertainties.Comment: 21 pages, 7 figures, LaTeX. Final version to be published in Phys. Rev.

Khovanov-Rozansky Homology and Topological Strings

We conjecture a relation between the sl(N) knot homology, recently introduced by Khovanov and Rozansky, and the spectrum of BPS states captured by open topological strings. This conjecture leads to new regularities among the sl(N) knot homology groups and suggests that they can be interpreted directly in topological string theory. We use this approach in various examples to predict the sl(N) knot homology groups for all values of N. We verify that our predictions pass some non-trivial checks.Comment: 25 pages, 2 figures, harvmac; minor corrections, references adde