Parking Garage Measurements Indicating a Gamma Spectrometer-Neutron Counter Background Correlation

Gamma spectroscopy and gross neutron counting systems are commonly employed together during nuclear security operations, particularly mobile searches. The data from these systems are typically analyzed independently; however, operational data suggest a relationship between the background signals from both systems. This relationship appears to occur between the neutron count rate and the high energy (greater than 3 MeV) gamma spectrometer count rate for background conditions. To verify the presence of this correlation, high energy gamma ray and neutron count rates were measured in five parking garages on the Texas A&M University campus. These measurements employed one 4” x 4” x 16” NaI detector with an Ortec digiBASE MCA, four moderated 1” x 18” 3He tubes (part of the Ortec NAI-SS system), and two unmoderated 2” x 15” 3He tubes (part of a Thermo PackEye system). The NaI detector was calibrated to a 50 MeV scale and ignored signals less than 4 MeV. Results show a decrease in the count rate of each detector as the systems were moved further below the roof of each garage. These decreases appear linear, but exponential relationships are plausible. More importantly, the data also show that the count rates from the two different detector types are linearly related. The linear relationships are strong, and the slopes vary depending on which neutron counter is considered against the gamma spectrometer. While it is possible that these relationships are the result of the NaI system detecting 4-50 MeV (or even higher energy) gamma rays, it is more likely that this system is detecting charged particles: muons generated by cosmic ray interactions with the atmosphere or protons produced by the decay of free neutrons. All three of these particles would be produced proportionally to the neutrons that the 3He tubes would detect, either from the decay of the neutrons themselves or by being produced from the same cosmic ray interactions that produce the neutrons

X-ray structure determination using low-resolution electron microscopy maps for molecular replacement

Structures of multisubunit macromolecular machines are primarily determined either by electron microscopy (EM) or by X-ray crystallography. In many cases, a structure for a complex can be obtained at low resolution (at a coarse level of detail) with EM and at a higher resolution (with finer detail) by X-ray crystallography. The integration of these two structural techniques is becoming increasingly important for the generation of atomic models of macromolecular complexes. A low-resolution EM image can be a powerful tool for obtaining the 'phase' information that is missing from an X-ray crystallography experiment; however, integration of EM and X-ray diffraction data has been technically challenging. Here we present a step-by-step protocol that explains how low-resolution EM maps can be placed in the crystallographic unit cell by molecular replacement, and how initial phases computed from the placed EM density are extended to high resolution by averaging maps over noncrystallographic symmetry. As the resolution gap between EM and X-ray crystallography continues to narrow, the use of EM maps to help with X-ray crystal structure determination, as described in this protocol, will become increasingly effective.R.N.J. is supported by the National Research Service Award postdoctoral fellowship (F32 GM108436) from the US National Institutes of Health (NIH). R.J.R. and T.C.T. are supported by a grant (GM063210) from the NIH. R.J.R. is supported by a Principal Research Fellowship from the Wellcome Trust (grant no. 082961/Z/07/Z). Research in the Wiedenheft lab is supported by the NIH IDeA Program COBRE (GM110732), an R01 to B.W. (GM108888), the National Science Foundation EPSCoR (EPS-110134), the M.J. Murdock Charitable Trust and the Montana State University Agricultural Experimental Station.This is the author accepted manuscript. The final version is available from NPG via http://dx.doi.org/10.1038/nprot.2015.06

Climatology of the Southeastern United States Continental Shelf Waters

Data from 2872 hydrographic stations have been used to determine the oceanographie climatology of the southeastern United States continental shelf waters. The data were sorted by each degree of latitude and by depth into three zones (0–20 m, 21–40 m, 41–60 m). Inner shelf water temperatures were similar to adjacent land air temperatures, while outer shelf temperatures were moderated by the Gulf Stream. Minimum and maximum water temperatures occurred in Georgia and South Carolina inner shelf water. Bottom temperatures were unusually low off Florida in the summer probably because of shelf break upwelling. Surface salinity was lowest adjacent to the rivers and reached minimums in the spring at the time of high runoff. An exception to this occurred in the fall, when strong southward winds apparently advected low salinity coastal water southward and offshore flow was restricted. Heat flux was calculated from changes in monthly mean depth-averaged inner shelf water temperatures. Heating occurred from March through July with maximum rates of 103 W m−2. Cooling occurred from October through February with maximum rates of −90 W m−2. Bulk stratification was estimated from the difference in near-surface and near-bottom monthly mean density. In the spring, stratification increases in inner shelf areas because of decreasing winds and increasing heat flux and runoff. By summer the whole shelf is highly stratified reflecting the contrast between high surface water temperatures and cooler bottom waters. Highest bulk stratification is found over the outer shelf. Stratification decreased with the approach offall with the associated cooling and high winds. Mean flow at midshelf was northward and appears to be produced by an along-shelf slope of sea level of oceanic origin

Hydrographic Variability of Southeastern United States Shelf and Slope Waters During the Genesis of Atlantic Lows Experiment: Winter 1986

Continental shelf waters are particularly responsive to winter storm events mainly because of their shallow depths. Those of the southeastern United States (the South Atlantic Bight (SAB)) are especially responsive because they are broad and shallow. Also, the Gulf Stream serves as a continual source of warm water at the outer boundary. Thus the SAB receives strong meteorological (wind stress and heat loss) and oceanographic (advective) forcing. During the Genesis of Atlantic Lows Experiment (GALE) the response of shelf waters to winter storm events and Gulf Stream forcing was observed. The mean conditions showed a mixed water column with areas of stratification near the coast and at the shelf break. The nearshore area was stratified only during weak offshore winds, and the shelf break area was stratified during southward winds with accompanying onshore Ekman flow. On the inner shelf, advective buoyancy flux was similar in value to heat flux buoyancy and the buoyancy equivalent of wind mixing. Over the shelf break the advective buoyancy flux was 4 times the other forms of buoyancy flux and controlled the observed potential energy variability. A simple box model heat budget used to separate the effect of Gulf Stream eddies and meanders, and Ekman flow and air‐sea heat exchange on the shelf heat content showed that the observed heat content variability was caused by intrusion of Gulf Stream water. The intrusions may be caused either by onshore Ekman flow during southward winds or Gulf Stream meander events

Infra-red divergences in plane wave backgrounds

We show that the emission of soft photons via nonlinear Compton scattering in a pulsed plane wave (laser field) is in general infra-red divergent. We give examples of both soft and soft-collinear divergences, and we pay particular attention to the case of crossed fields in both classical and quantum theories.Comment: 15 pages, 7 figure

Faking like a woman? Towards an interpretative theorization of sexual pleasure.

This article explores the possibility of developing a feminist approach to gendered and sexual embodiment which is rooted in the pragmatist/interactionist tradition derived from G.H. Mead, but which in turn develops this perspective by inflecting it through more recent feminist thinking. In so doing we seek to rebalance some of the rather abstract work on gender and embodiment by focusing on an instance of 'heterosexual' everyday/night life - the production of the female orgasm. Through engaging with feminist and interactionist work, we develop an approach to embodied sexual pleasure that emphasizes the sociality of sexual practices and of reflexive sexual selves. We argue that sexual practices and experiences must be understood in social context, taking account of the situatedness of sex as well as wider socio-cultural processes the production of sexual desire and sexual pleasure (or their non-production) always entails interpretive, interactional processes

Direct tissue analysis of phospholipids in rat brain using MALDI-TOFMS and MALDI-ion mobility-TOFMS

After water, lipids are the most common biomolecules found in the brain (12%). A brief perusal of the physiology, anatomy, and pathophysiology of the brain illustrates the importance of lipids. Recent advances in mass spectrometry have allowed the direct probing of tissues. However, most studies have focused on proteins. In the present work, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and MALDI-ion mobility (IM)-TOFMS were employed for direct analysis of phospholipids in rat brain tissue. Molecular ions (MH+) corresponding to phosphatidylcholines, phosphatidylethanolamines, and sphingomyelin, were recorded. When studying pharmacology, we learn that many therapeutic compounds are stored in the body’s adipose tissue. MALDI-TOFMS and MALDI- IM-TOFMS were thus used to analyze rat brain tissue with chlorisondamine added directly onto the tissue slice. With both techniques, noncovalent complexes between the tissue phospholipids and chlorisondamine were detected. In addition, MALDI-IM-TOFMS of noncovalent complexes between phospholipids and chlorisondamine displayed a mobility between that of an isobaric lipid and peptide

Local lung hypoxia determines epithelial fate decisions during alveolar regeneration.

After influenza infection, lineage-negative epithelial progenitors (LNEPs) exhibit a binary response to reconstitute epithelial barriers: activating a Notch-dependent ΔNp63/cytokeratin 5 (Krt5) remodelling program or differentiating into alveolar type II cells (AEC2s). Here we show that local lung hypoxia, through hypoxia-inducible factor (HIF1α), drives Notch signalling and Krt5pos basal-like cell expansion. Single-cell transcriptional profiling of human AEC2s from fibrotic lungs revealed a hypoxic subpopulation with activated Notch, suppressed surfactant protein C (SPC), and transdifferentiation toward a Krt5pos basal-like state. Activated murine Krt5pos LNEPs and diseased human AEC2s upregulate strikingly similar core pathways underlying migration and squamous metaplasia. While robust, HIF1α-driven metaplasia is ultimately inferior to AEC2 reconstitution in restoring normal lung function. HIF1α deletion or enhanced Wnt/β-catenin activity in Sox2pos LNEPs blocks Notch and Krt5 activation, instead promoting rapid AEC2 differentiation and migration and improving the quality of alveolar repair