Impact of high dietary plant protein with or without marine ingredients in gut mucosa proteome of gilthead seabream (Sparus aurata, L.)

[EN] The digestive tract, particularly the intestine, represents one of the main sites of interactions with the environment, playing the gut mucosa a crucial role in the digestion and absorption of nutrients, and in the immune defence. Previous researches have proven that the fishmeal replacement by plant sources could have an impact on the intestinal status at both digestive and immune level, compromising relevant productive parameters, such as feed efficiency, growth or survival. In order to evaluate the long-term impact of total fishmeal replacement on intestinal mucosa, the gut mucosa proteome was analysed in fish fed with a fishmeal-based diet, against plant protein-based diets with or without alternative marine sources inclusion. Total fishmeal replacement without marine ingredients inclusion, reported a negative impact in growth and biometric parameters, further an altered gut mucosa proteome. However, the inclusion of a low percentage of marine ingredients in plant protein-based diets was able to maintain the growth, biometrics parameters and gut mucosa proteome with similar values to FM group. A total fishmeal replacement induced a big set of underrepresented proteins in relation to several biological processes such as intracellular transport, assembly of cellular macrocomplex, protein localization and protein catabolism, as well as several molecular functions, mainly related with binding to different molecules and the maintenance of the cytoskeleton structure. The set of downregulated proteins also included molecules which have a crucial role in the maintenance of the normal function of the enterocytes, and therefore, of the epithelium, including permeability, immune and inflammatory response regulation and nutritional absorption. Possibly, the amino acid imbalance presented in VM diet, in a long-term feeding, may be the main reason of these alterations, which can be prevented by the inclusion of 15% of alternative marine sources. Significance: Long-term feeding with plant protein based diets may be considered as a stress factor and lead to a negative impact on digestive and immune system mechanisms at the gut, that can become apparent in a reduced fish performance. The need for fishmeal replacement by alternative ingredients such as plant sources to ensure the sustainability of the aquaculture sector has led the research assessing the intestinal status of fish to be of increasing importance. This scientific work provides further knowledge about the proteins and biologic processes altered in the gut in response to plant protein based diets, suggesting the loss of part of gut mucosa functionality. Nevertheless, the inclusion of alternative marine ingredients was able to reverse these negative effects, showing as a feasible option to develop sustainable aquafeeds.The first author was supported by a contract-grant (Contrato Pre doctoral para la Formacion de Profesorado Universitario) from Subprogramas de Formacion y Movilidad within the Programa Estatal de Promocion del Talento y su Empleabilidad of the Ministerio de Educacion, Cultura y Deporte of Spain.Estruch, G.; Martínez-Llorens, S.; Tomas-Vidal, A.; Monge-Ortiz, R.; Jover Cerda, M.; Brown, PB.; Peñaranda, D. (2020). Impact of high dietary plant protein with or without marine ingredients in gut mucosa proteome of gilthead seabream (Sparus aurata, L.). Journal of Proteomics. 216:1-13. https://doi.org/10.1016/j.jprot.2020.103672S113216Martínez-Llorens, S., Moñino, A. V., Tomás Vidal, A., Salvador, V. J. M., Pla Torres, M., & Jover Cerdá, M. (2007). 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Detectors with an electroluminesence readout show an excellence performance in respect of energy resolution making them interesting for various applications as X-ray detection, double beta and dark matter experiments, Compton and gamma cameras, etc. In the following the study of a readout based on avalanche photo diodes to detect directly the VUV photons is presented. Results of measurements with 5 APDs in xenon at pressures between 1 and 1.65 bar are shown indicating that such a readout can provide excellent energy and a moderate position resolution.Comment: Talk given at the 5th Symposium on Large TPCs for Low Energy Rare Events. Submitted to JPCS (Journal of Physics: Conference Series) for publication. 8 pages, 17 figure

Filipino Students’ Standpoint on Going Back to Traditional Schooling in the New Normal

Schools worldwide have started opening doors to welcome back students who, for almost two years, have been stuck studying at home. This study looks at the standpoint of Filipino students on going back to regular face-to-face schooling. There were 2,274 students of different tiers of education (high school, collegiate, graduate) from different major island groups of the Philippines (Luzon, Visayas, Mindanao) who participated in the study. The study used a mixed-method of descriptive statistics to present the quantitative data gathered and thematic analysis of qualitative responses from the subjects. The majority of all the respondents favored going back to the physical classroom, and little only favored staying using the distance mode. In the qualitative analysis, the recurring reasons of the students varied from personal, economic, and fear of getting the virus. It was concluded that Filipino students want to go back to schooling. Moreover, a sizeable amount preferred hybrid while a small number preferred to stay in online or distance mode. Educational institutions should always observe the covid 19 protocol when students go back

Preventing and addressing the stress reactions of health care workers caring for patients with COVID-19: Development of a digital platform (Be + against COVID)

Background: COVID-19 became a major public health concern in March 2020. Due to the high rate of hospitalizations for COVID-19 in a short time, health care workers and other involved staff are subjected to a large workload and high emotional distress. Objective: The objective of this study is to develop a digital tool to provide support resources that might prevent and consider acute stress reactions in health care workers and other support staff due to the COVID-19 pandemic. Methods: The contents of the digital platform were created through an evidence-based review and consensus conference. The website was built using the Google Blogger tool. The Android version of the app was developed in the Java and XML languages using Android Studio version 3.6, and the iOS version was developed in the Swift language using Xcode version 11.5. The app was evaluated externally by the Andalusian Agency for Healthcare Quality. Results: We detected the needs and pressing situations of frontline health care workers, and then, we proposed a serial of recommendations and support resources to address them. These resources were redesigned using the feedback received. A website in three different languages (Spanish, English, and Portuguese) and a mobile app were developed with these contents, and the AppSaludable Quality Seal was granted to the app. A specific self-report scale to measure acute stress and additional tools were included to support the health care workforce. This instrument has been used in several Latin American countries and has been adapted considering cultural differences. The resources section of the website was the most visited with 18, 516 out of 68, 913 (26.9%) visits, and the “Self-Report Acute Stress Scale” was the most visited resource with 6468 out of 18, 516 (34.9%) visits. Conclusions: The Be + against COVID platform (website and app) was developed and launched to offer a pool of recommendations and support resources, which were specifically designed to protect the psychological well-being and the work morale of health care workers. This is an original initiative different from the usual psychological assistance hotlines

¿Vamos a la Huerta? : Recuperación de saberes culturales alrededor de la producción agroecológica de alimentos

Con el avance de la agricultura industrial, los pueblos rurales fueron vaciados de saberes culturales, como es la autoproducción de alimentos. Gobernador Ugarte, ubicado en el partido de 25 de Mayo, provincia de Buenos Aires, Argentina, no escapa a esta problemática ambiental, social y cultural, agravada por el hecho de que no tiene verdulerías, que las pocas verduras y frutas que llegan son muy caras y; que la ciudad más próxima queda a 40km. Contrariamente a lo que se piensa en las ciudades, los vecinos no saben hacer huertas, porque se perdió la transmisión cultural de boca a oreja sobre la producción de alimentos. ¿Vamos a la Huerta?, es una propuesta lúdica y comunicacional para el aprendizaje de producción de hortalizas con bases agroecológicas, que se inició en el año 2008, y que realizan los alumnos del ciclo superior de la Escuela Secundaria, los cuales diseñan juegos para que los alumnos del nivel primario e inicial, recuperen dichos saberes desde una mirada interdisciplinar y reconstruyan la relación entre ciencias y saberes culturales.Eje: B5 Sistemas de conocimiento (Relatos de experiencias)Facultad de Ciencias Agrarias y Forestale