Genetic Overexpression of NR2B Subunit Enhances Social Recognition Memory for Different Strains and Species

The ability to learn and remember conspecifics is essential for the establishment and maintenance of social groups. Many animals, including humans, primates and rodents, depend on stable social relationships for survival. Social learning and social recognition have become emerging areas of interest for neuroscientists but are still not well understood. It has been established that several hormones play a role in the modulation of social recognition including estrogen, oxytocin and arginine vasopression. Relatively few studies have investigated how social recognition might be improved or enhanced. In this study, we investigate the role of the NMDA receptor in social recognition memory, specifically the consequences of altering the ratio of the NR2B∶NR2A subunits in the forebrain regions in social behavior. We produced transgenic mice in which the NR2B subunit of the NMDA receptor was overexpressed postnatally in the excitatory neurons of the forebrain areas including the cortex, amygdala and hippocampus. We investigated the ability of both our transgenic animals and their wild-type littermate to learn and remember juvenile conspecifics using both 1-hr and 24-hr memory tests. Our experiments show that the wild-type animals and NR2B transgenic mice preformed similarly in the 1-hr test. However, transgenic mice showed better performances in 24-hr tests of recognizing animals of a different strain or animals of a different species. We conclude that NR2B overexpression in the forebrain enhances social recognition memory for different strains and animal species

Hippocampal Volume Reduction in Congenital Central Hypoventilation Syndrome

Children with congenital central hypoventilation syndrome (CCHS), a genetic disorder characterized by diminished drive to breathe during sleep and impaired CO2 sensitivity, show brain structural and functional changes on magnetic resonance imaging (MRI) scans, with impaired responses in specific hippocampal regions, suggesting localized injury

Nerve growth factor induces neurite outgrowth of PC12 cells by promoting Gβγ-microtubule interaction

Background: Assembly and disassembly of microtubules (MTs) is critical for neurite outgrowth and differentiation. Evidence suggests that nerve growth factor (NGF) induces neurite outgrowth from PC12 cells by activating the receptor tyrosine kinase, TrkA. G protein-coupled receptors (GPCRs) as well as heterotrimeric G proteins are also involved in regulating neurite outgrowth. However, the possible connection between these pathways and how they might ultimately converge to regulate the assembly and organization of MTs during neurite outgrowth is not well understood. Results: Here, we report that Gβγ, an important component of the GPCR pathway, is critical for NGF-induced neuronal differentiation of PC12 cells. We have found that NGF promoted the interaction of Gβγ with MTs and stimulated MT assembly. While Gβγ-sequestering peptide GRK2i inhibited neurite formation, disrupted MTs, and induced neurite damage, the Gβγ activator mSIRK stimulated neurite outgrowth, which indicates the involvement of Gβγ in this process. Because we have shown earlier that prenylation and subsequent methylation/demethylation of γ subunits are required for the Gβγ-MTs interaction in vitro, small-molecule inhibitors (L-28 and L-23) targeting prenylated methylated protein methyl esterase (PMPMEase) were tested in the current study. We found that these inhibitors disrupted Gβγ and ΜΤ organization and affected cellular morphology and neurite outgrowth. In further support of a role of Gβγ-MT interaction in neuronal differentiation, it was observed that overexpression of Gβγ in PC12 cells induced neurite outgrowth in the absence of added NGF. Moreover, overexpressed Gβγ exhibited a pattern of association with MTs similar to that observed in NGF-differentiated cells. Conclusions: Altogether, our results demonstrate that βγ subunit of heterotrimeric G proteins play a critical role in neurite outgrowth and differentiation by interacting with MTs and modulating MT rearrangement. Electronic supplementary material The online version of this article (doi:10.1186/s12868-014-0132-4) contains supplementary material, which is available to authorized users

The 'long' 16th century : a key period of animal husbandry change in England

Although many historians have extensively discussed the agricultural history of England between the Late Middle Ages and the Modern Era, this period of crucial changes has received less attention by archaeologists. In this paper, zooarchaeological evidence dated between the Late Middle Ages and the Early Modern period is analysed to investigate changes in animal husbandry during the ‘long’ sixteenth century. The size and shape of the main domestic animals (cattle, sheep, pig and chicken) is explored through biometrical data and discussed in line with evidence of taxonomic frequencies, ageing and sex ratios. Data from 12 sites with relevant chronologies and located in different areas of the country are considered. The results show that, although a remarkable size increase of animals occurred in England throughout the post-medieval period, much of this improvement occurred as early as the sixteenth century. The nature and causes of such improvement are discussed, with the aim of understanding the development of Early Modern farming and the foundations of the so-called Agricultural Revolution

Mangrove-forest evolution in a sediment-rich estuarine system: opportunists or agents of geomorphic change?

The majority of the world's mangrove forests occur on mostly mineral sediments of fluvial origin. Two perspectives exist on the biogeomorphic development of these forests, i.e. that mangroves are opportunistic, with forest development primarily driven by physical processes, or alternatively that biophysical feedbacks strongly influence sedimentation and resulting geomorphology. On the Firth of Thames coast, New Zealand, we evaluate these two possible scenarios for sediment accumulation and forest development using high-resolution sedimentary records and a detailed chronology of mangrove-forest (Avicennia marina) development since the 1950s. Cores were collected along a shore-normal transect of known elevation relative to mean sea level (MSL). Activities for lead-210 (Pb-210), caesium-137 (Cs-137) and beryllium-7 (Be-7), and sediment properties were analysed, with Pb-210 sediment accumulation rates (SARs), compensated for deep subsidence (similar to 8 mm yr(-1)) used as a proxy for elevation gain. At least four phases of forest development since the 1950s are recognized. An old-growth forest developed by the late-1970s with more recent seaward forest expansion thereafter. Excess 210Pb profiles from the old-growth forest exhibit relatively low SARs near the top (7-12 mm yr(-1)) and bottom (10-22 mm yr(-1)) of cores, separated by an interval of higher SARs (33-100 mm yr(-1)). A general trend of increasing SAR over time characterizes the recent forest. Biogeomorphic evolution of the system is more complex than simple mudflat accretion/progradation and mangrove-forest expansion. Surface-elevation gain in the old-growth forest displays an asymptotic trajectory, with a secondary depocentre developing on the seaward mudflat from the mid-1970s. Two-to ten-fold increases in 210Pb SARs are unambiguously large and occurred years to decades before seedling recruitment, demonstrating that mangroves do not measurably enhance sedimentation over annual to decadal timescales. This suggests that mangrove-forest development is largely dependent on physical processes, with forests occupying mudflats once they reach a suitable elevation in the intertidal. Copyright (C) 2015 John Wiley & Sons, Ltd