Hybrid Model for the Analysis of Human Gait: A Non-linear Approach

In this work, a generalization of the study of the human gait was made from already existent models in the literature, like models of Keller and Kockshenev. In this hybrid model, a strategy of metabolic energy minimization is combined in a race process, with a non-linear description of the movement of the mass center’s libration, trying to reproduce the behavior of the walk-run transition. The results of the experimental data, for different speed regimes, indicate that the perimeter of the trajectory of the mass center is a relevant quantity in the quantification of this dynamic. An experimental procedure was put into practice in collaboration with the research group in Biomedical Engineering, Basic Sciences and Laboratories of the Manuela Beltrán University in Bogotá, Colombia

Cycle-to-cycle combustion variability modelling in spark ignited engines for control purposes

This is the author's version of a work that was accepted for publication in International Journal of Engine Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published as https://doi.org/10.1177/1468087419885754.[EN] A control-oriented model of spark ignition combustion is presented. The model makes use of avaliable signals, such as spark advance, air mass, intake pressure, and lambda, to characterize not only the average combustion evolution but also the cycle-to-cycle variability. The conventional turbulent flame propagation model with two states, namely entrained mass and burnt mass, is improved by look-up tables at some parameters, and the cycle-to-cycle variability is estimated by propagation of an exogenous noise with a normal probabilistic distribution at the turbulent and laminar flame speed, which intends to simulate the unknowns at turbulent flow, temperature distribution, or initial kernel distribution. The model is able to estimate which is the expected variability during the combustion evolution and might be used online for characterizing the time response of closed-loop control actions or it can be used offline to improve the control strategies without large experimental test campaigns. Experimental data from a four-stroke commercial engine was used for calibration and validation purposes, demonstrating the capabilities of the model in steady and transient conditions.The authors appreciate the technical support and the clues given by J. Israel Sanchez for the model development and also acknowledge the support of Spanish Ministerio de Economia, Industria y Competitividad through project TRA2016-78717-R.Pla Moreno, B.; De La Morena, J.; Bares-Moreno, P.; Jimenez, IA. (2020). Cycle-to-cycle combustion variability modelling in spark ignited engines for control purposes. International Journal of Engine Research. 21(8):1398-1411. https://doi.org/10.1177/1468087419885754S13981411218Wang, S., Prucka, R., Zhu, Q., Prucka, M., & Dourra, H. (2016). A Real-Time Model for Spark Ignition Engine Combustion Phasing Prediction. SAE International Journal of Engines, 9(2), 1180-1190. doi:10.4271/2016-01-0819Kim, N., Ko, I., & Min, K. (2018). Development of a zero-dimensional turbulence model for a spark ignition engine. International Journal of Engine Research, 20(4), 441-451. doi:10.1177/1468087418760406Wang, S., Zhu, Q., Prucka, R., Prucka, M., & Dourra, H. (2015). Input Adaptation for Control Oriented Physics-Based SI Engine Combustion Models Based on Cylinder Pressure Feedback. SAE International Journal of Engines, 8(4), 1463-1471. doi:10.4271/2015-01-0877Zhen, X., Wang, Y., Xu, S., Zhu, Y., Tao, C., Xu, T., & Song, M. (2012). The engine knock analysis – An overview. Applied Energy, 92, 628-636. doi:10.1016/j.apenergy.2011.11.079Bares, P., Selmanaj, D., Guardiola, C., & Onder, C. (2018). Knock probability estimation through an in-cylinder temperature model with exogenous noise. Mechanical Systems and Signal Processing, 98, 756-769. doi:10.1016/j.ymssp.2017.05.033Zhang, Y., Shen, X., Wu, Y., & Shen, T. (2019). On-board knock probability map learning–based spark advance control for combustion engines. International Journal of Engine Research, 20(10), 1073-1088. doi:10.1177/1468087419858026Spelina, J. M., Peyton Jones, J. C., & Frey, J. (2014). Stochastic simulation and analysis of a classical knock controller. International Journal of Engine Research, 16(3), 461-473. doi:10.1177/1468087414551073Neumann, D., Jörg, C., Peschke, N., Schaub, J., & Schnorbus, T. (2017). Real-time capable simulation of diesel combustion processes for HiL applications. International Journal of Engine Research, 19(2), 214-229. doi:10.1177/1468087417726226Pipitone, E. (2008). A Comparison Between Combustion Phase Indicators for Optimal Spark Timing. Journal of Engineering for Gas Turbines and Power, 130(5). doi:10.1115/1.2939012Bares, P., Selmanaj, D., Guardiola, C., & Onder, C. (2018). A new knock event definition for knock detection and control optimization. Applied Thermal Engineering, 131, 80-88. doi:10.1016/j.applthermaleng.2017.11.138Peyton Jones, J. C., Spelina, J. M., & Frey, J. (2013). Optimizing knock thresholds for improved knock control. International Journal of Engine Research, 15(1), 123-132. doi:10.1177/1468087413482321Emiliano, P. (2014). Spark Ignition Feedback Control by Means of Combustion Phase Indicators on Steady and Transient Operation. Journal of Dynamic Systems, Measurement, and Control, 136(5). doi:10.1115/1.4026966Zhu, Q., Prucka, R., Wang, S., Prucka, M., & Dourra, H. (2016). Model-Based Optimal Combustion Phasing Control Strategy for Spark Ignition Engines. SAE International Journal of Engines, 9(2), 1170-1179. doi:10.4271/2016-01-0818Zhang, Y., & Shen, T. (2017). Cylinder pressure based combustion phase optimization and control in spark-ignited engines. Control Theory and Technology, 15(2), 83-91. doi:10.1007/s11768-017-6175-1Zhang, Y., Shen, X., & Shen, T. (2018). A survey on online learning and optimization for spark advance control of SI engines. Science China Information Sciences, 61(7). doi:10.1007/s11432-017-9377-7Corti, E., Forte, C., Mancini, G., & Moro, D. (2014). Automatic Combustion Phase Calibration With Extremum Seeking Approach. Journal of Engineering for Gas Turbines and Power, 136(9). doi:10.1115/1.4027188Corti, E., Cerofolini, A., Cavina, N., Forte, C., Mancini, G., Moro, D., … Ravaglioli, V. (2014). Automatic Calibration of Control Parameters based on Merit Function Spectral Analysis. Energy Procedia, 45, 919-928. doi:10.1016/j.egypro.2014.01.097Popovic, D., Jankovic, M., Magner, S., & Teel, A. R. (2006). Extremum seeking methods for optimization of variable cam timing engine operation. IEEE Transactions on Control Systems Technology, 14(3), 398-407. doi:10.1109/tcst.2005.863660Hellstrom, E., Lee, D., Jiang, L., Stefanopoulou, A. G., & Yilmaz, H. (2013). On-Board Calibration of Spark Timing by Extremum Seeking for Flex-Fuel Engines. IEEE Transactions on Control Systems Technology, 21(6), 2273-2279. doi:10.1109/tcst.2012.2236093Pera, C., Chevillard, S., & Reveillon, J. (2013). Effects of residual burnt gas heterogeneity on early flame propagation and on cyclic variability in spark-ignited engines. Combustion and Flame, 160(6), 1020-1032. doi:10.1016/j.combustflame.2013.01.009Zhao, L., Moiz, A. A., Som, S., Fogla, N., Bybee, M., Wahiduzzaman, S., … Kodavasal, J. (2017). Examining the role of flame topologies and in-cylinder flow fields on cyclic variability in spark-ignited engines using large-eddy simulation. International Journal of Engine Research, 19(8), 886-904. doi:10.1177/1468087417732447Pera, C., Knop, V., & Reveillon, J. (2015). Influence of flow and ignition fluctuations on cycle-to-cycle variations in early flame kernel growth. Proceedings of the Combustion Institute, 35(3), 2897-2905. doi:10.1016/j.proci.2014.07.037Schiffmann, P., Reuss, D. L., & Sick, V. (2017). Empirical investigation of spark-ignited flame-initiation cycle-to-cycle variability in a homogeneous charge reciprocating engine. International Journal of Engine Research, 19(5), 491-508. doi:10.1177/1468087417720558Galloni, E. (2009). Analyses about parameters that affect cyclic variation in a spark ignition engine. Applied Thermal Engineering, 29(5-6), 1131-1137. doi:10.1016/j.applthermaleng.2008.06.001Tamaki, S., Sakayanagi, Y., Sekiguchi, K., Ibuki, T., Tahara, K., & Sampei, M. (2014). On-line Feedforward Map Generation for Engine Ignition Timing Control. IFAC Proceedings Volumes, 47(3), 5691-5696. doi:10.3182/20140824-6-za-1003.01886Zhang, Y., & Shen, T. (2018). Combustion Variation Feedback Control Approach for Multi-cylinder Spark Ignition Engines. IFAC-PapersOnLine, 51(31), 105-110. doi:10.1016/j.ifacol.2018.10.020Corti, E., & Forte, C. (2011). Spark Advance Real-Time Optimization Based on Combustion Analysis. Journal of Engineering for Gas Turbines and Power, 133(9). doi:10.1115/1.4002919Gao, J., Wu, Y., & Shen, T. (2016). Experimental comparisons of hypothesis test and moving average based combustion phase controllers. ISA Transactions, 65, 504-515. doi:10.1016/j.isatra.2016.09.003Gao, J., Wu, Y., & Shen, T. (2017). A statistical combustion phase control approach of SI engines. Mechanical Systems and Signal Processing, 85, 218-235. doi:10.1016/j.ymssp.2016.08.007Lee, D., Jiang, L., Yilmaz, H., & Stefanopoulou, A. G. (2010). Preliminary Results on Optimal Variable Valve Timing and Spark Timing Control via Extremum Seeking. IFAC Proceedings Volumes, 43(18), 377-384. doi:10.3182/20100913-3-us-2015.00038Di Mauro, A., Chen, H., & Sick, V. (2019). Neural network prediction of cycle-to-cycle power variability in a spark-ignited internal combustion engine. Proceedings of the Combustion Institute, 37(4), 4937-4944. doi:10.1016/j.proci.2018.08.058Lapuerta, M., Armas, O., & Hernández, J. J. (1999). Diagnosis of DI Diesel combustion from in-cylinder pressure signal by estimation of mean thermodynamic properties of the gas. Applied Thermal Engineering, 19(5), 513-529. doi:10.1016/s1359-4311(98)00075-1Ceviz, M. A., & Kaymaz, İ. (2005). Temperature and air–fuel ratio dependent specific heat ratio functions for lean burned and unburned mixture. Energy Conversion and Management, 46(15-16), 2387-2404. doi:10.1016/j.enconman.2004.12.009Guardiola, C., Triantopoulos, V., Bares, P., Bohac, S., & Stefanopoulou, A. (2016). Simultaneous Estimation of Intake and Residual Mass Using In-Cylinder Pressure in an Engine with Negative Valve Overlap. IFAC-PapersOnLine, 49(11), 461-468. doi:10.1016/j.ifacol.2016.08.068Wang, S., Prucka, R., Prucka, M., & Dourra, H. (2014). Control-oriented residual gas mass prediction for spark ignition engines. International Journal of Engine Research, 16(7), 897-907. doi:10.1177/1468087414555732Keck, J. C. (1982). Turbulent flame structure and speed in spark-ignition engines. Symposium (International) on Combustion, 19(1), 1451-1466. doi:10.1016/s0082-0784(82)80322-

When One Hemisphere Takes Control: Metacontrol in Pigeons (Columba livia)

Vertebrate brains are composed of two hemispheres that receive input, compute, and interact to form a unified response. How the partially different processes of both hemispheres are integrated to create a single output is largely unknown. In some cases one hemisphere takes charge of the response selection--a process known as metacontrol. Thus far, this phenomenon has only been shown in a handful of studies with primates, mostly conducted in humans. Metacontrol, however, is even more relevant for animals like birds with laterally placed eyes and complete chiasmatic decussation since visual input to the hemispheres is largely different.Homing pigeons (Columba livia) were trained with a color discrimination task. Each hemisphere was trained with a different color pair and therefore had a different experience. Subsequently, the pigeons were binocularly examined with two additional stimuli that combined the positive color of one hemisphere with a negative color that had been shown to the other, omitting the availability of a coherent solution and confronting the pigeons with a conflicting situation. Some of the pigeons responded to both stimuli, indicating that none of the hemispheres dominated the overall preference. Some birds, however, responded primarily to one of the conflicting stimuli, showing that they based their choice on the left- or right-monocularly learned color pair, indicating hemispheric metacontrol.We could demonstrate for the first time that metacontrol is a widespread phenomenon that also exists in birds, and thus in principle requires no corpus callosum. Our results are closely similar to those in humans: monocular performance was higher than binocular one and animals displayed different modes of hemispheric dominance. Thus, metacontrol is a dynamic and widely distributed process that possibly constitutes a requirement for all animals with a bipartite brain to confront the problem of choosing between two hemisphere-bound behavioral options

New zebrafish models of neurodegeneration

In modern biomedicine, the increasing need to develop experimental models to further our understanding of disease conditions and delineate innovative treatments has found in the zebrafish (Danio rerio) an experimental model, and indeed a valuable asset, to close the gap between in vitro and in vivo assays. Translation of ideas at a faster pace is vital in the field of neurodegeneration, with the attempt to slow or prevent the dramatic impact on the society's welfare being an essential priority. Our research group has pioneered the use of zebrafish to contribute to the quest for faster and improved understanding and treatment of neurodegeneration in concert with, and inspired by, many others who have primed the study of the zebrafish to understand and search for a cure for disorders of the nervous system. Aware of the many advantages this vertebrate model holds, here, we present an update on the recent zebrafish models available to study neurodegeneration with the goal of stimulating further interest and increasing the number of diseases and applications for which they can be exploited. We shall do so by citing and commenting on recent breakthroughs made possible via zebrafish, highlighting their benefits for the testing of therapeutics and dissecting of disease mechanisms

Paradoxical effects of Worrisome Thoughts Suppression: the influence of depressive mood

Thought suppression increases the persistence of unwanted idiosyncratic worries thoughts when individuals try to suppress them. The failure of suppression may contribute to the development and maintenance of emotional disorders. Depressive people seem particulary prone to engage in unsuccessful mental control strategies such as thought suppression. Worry has been reported to be elevated in depressed individuals and a dysphoric mood may also contribute for the failure of suppression. No studies examine, however, the suppression of worisome thoughts in individuals with depressive symptoms. To investigate the suppression effects of worrisome thoughts, 46 participants were selected according to the cut-off score of a depressive symptomatology scale and they were divided in two groups (subclinical and nonclinical group). All the individuals took part in an experimental paradigm of thought suppression. The results of the mixed factorial analysis of variance revealed an increased frequency of worrisome thoughts during the suppression phase on depending of the depressive symptoms. These findings confirm that depressive mood can reduce the success of suppression.info:eu-repo/semantics/publishedVersio

Interactions between downslope flows and a developing cold-air pool

A numerical model has been used to characterize the development of a region of enhanced cooling in an alpine valley with a width of order (Formula presented.) km, under decoupled stable conditions. The region of enhanced cooling develops largely as a region of relatively dry air which partitions the valley atmosphere dynamics into two volumes, with airflow partially trapped within the valley by a developing elevated inversion. Complex interactions between the region of enhanced cooling and the downslope flows are quantified. The cooling within the region of enhanced cooling and the elevated inversion is almost equally partitioned between radiative and dynamic effects. By the end of the simulation, the different valley atmospheric regions approach a state of thermal equilibrium with one another, though this cannot be said of the valley atmosphere and its external environment.Peer reviewe

The WACMOS-ET project, part 2 : evaluation of global terrestrial evaporation data sets

The WAter Cycle Multi-mission Observation Strategy - EvapoTranspiration (WACMOS-ET) project aims to advance the development of land evaporation estimates on global and regional scales. Its main objective is the derivation, validation, and intercomparison of a group of existing evaporation retrieval algorithms driven by a common forcing data set. Three commonly used process-based evaporation methodologies are evaluated: the Penman-Monteith algorithm behind the official Moderate Resolution Imaging Spectroradiometer (MODIS) evaporation product (PM-MOD), the Global Land Evaporation Amsterdam Model (GLEAM), and the Priestley-Taylor Jet Propulsion Laboratory model (PT-JPL). The resulting global spatiotemporal variability of evaporation, the closure of regional water budgets, and the discrete estimation of land evaporation components or sources (i.e. transpiration, interception loss, and direct soil evaporation) are investigated using river discharge data, independent global evaporation data sets and results from previous studies. In a companion article (Part 1), Michel et al. (2016) inspect the performance of these three models at local scales using measurements from eddy-covariance towers and include in the assessment the Surface Energy Balance System (SEBS) model. In agreement with Part 1, our results indicate that the Priestley and Taylor products (PT-JPL and GLEAM) perform best overall for most ecosystems and climate regimes. While all three evaporation products adequately represent the expected average geographical patterns and seasonality, there is a tendency in PM-MOD to underestimate the flux in the tropics and subtropics. Overall, results from GLEAM and PT-JPL appear more realistic when compared to surface water balances from 837 globally distributed catchments and to separate evaporation estimates from ERA-Interim and the model tree ensemble (MTE). Nonetheless, all products show large dissimilarities during conditions of water stress and drought and deficiencies in the way evaporation is partitioned into its different components. This observed inter-product variability, even when common forcing is used, suggests that caution is necessary in applying a single data set for large-scale studies in isolation. A general finding that different models perform better under different conditions highlights the potential for considering biome- or climate-specific composites of models. Nevertheless, the generation of a multi-product ensemble, with weighting based on validation analyses and uncertainty assessments, is proposed as the best way forward in our long-term goal to develop a robust observational benchmark data set of continental evaporation

Pollutant dispersion in a developing valley cold-air pool

Pollutants are trapped and accumulate within cold-air pools, thereby affecting air quality. A numerical model is used to quantify the role of cold-air-pooling processes in the dispersion of air pollution in a developing cold-air pool within an alpine valley under decoupled stable conditions. Results indicate that the negatively buoyant downslope flows transport and mix pollutants into the valley to depths that depend on the temperature deficit of the flow and the ambient temperature structure inside the valley. Along the slopes, pollutants are generally entrained above the cold-air pool and detrained within the cold-air pool, largely above the ground-based inversion layer. The ability of the cold-air pool to dilute pollutants is quantified. The analysis shows that the downslope flows fill the valley with air from above, which is then largely trapped within the cold-air pool, and that dilution depends on where the pollutants are emitted with respect to the positions of the top of the ground-based inversion layer and cold-air pool, and on the slope wind speeds. Over the lower part of the slopes, the cold-air-pool-averaged concentrations are proportional to the slope wind speeds where the pollutants are emitted, and diminish as the cold-air pool deepens. Pollutants emitted within the ground-based inversion layer are largely trapped there. Pollutants emitted farther up the slopes detrain within the cold-air pool above the ground-based inversion layer, although some fraction, increasing with distance from the top of the slopes, penetrates into the ground-based inversion layer.Peer reviewe

Influence of homogenization conditions on physical properties and antioxidant activity of fully biodegradable pea protein-alpha-tocopherol films

In this study, antioxidant biodegradable films based on pea protein and alpha-tocopherol were successfully developed by solution casting. The effect of both the homogenization conditions (rotor stator and microfluidizer) and the relative humidity (RH) on the microstructure and physical properties (transparency, tensile, oxygen and water vapour barrier properties) of pea protein/alpha-tocopherol-based films was evaluated. The addition of alpha-tocopherol produced minimal changes in the films transparency, while providing them with antioxidant properties and improved water vapour and oxygen barrier properties (up to 30 % in both water vapour and oxygen permeability) when films were at low and intermediate RH. The addition of alpha-tocopherol in microfluidized films gave rise to an increase in their resistance to break and extensibility (up to 27 % in E values) at intermediate and high RH. These results add a new insight into the potential of employing pea protein and alpha-tocopherol in the development of fully biodegradable antioxidant films which are of interest in food packagingThe authors acknowledge the financial support from the Spanish Ministerio de Educacion y Ciencia throughout the project AGL2010-20694, co-funded by FEDER. Author M.J.Fabra is a recipient of a Juan de la Cierva contract from the Spanish Ministerio de Economia y Competitividad.Fabra, MJ.; Jiménez, A.; Talens Oliag, P.; Chiralt, A. (2014). Influence of homogenization conditions on physical properties and antioxidant activity of fully biodegradable pea protein-alpha-tocopherol films. Food and Bioprocess Technology. 7(12):3569-3578. https://doi.org/10.1007/s11947-014-1372-0S35693578712ASTM (1995). Standard test methods for water vapor transmission of materials. Standards Desingnations: E96-95. In: Annual Book of ASTM Standards (pp. 406-413); American Society for Testing and Materials: Philadelphia, PA.ASTM (2001). Standard test method for tensile properties of thin plastic sheeting. Standard D882. In: Annual book of American Standard Testing Methods (pp 162-170). D882. Philadelphia:ASTM.Bertan, L. C., Tanada-Palmu, P. S., Siani, A. C., & Grosso, C. R. F. (2005). Effect of fatty acids and “Brazilian elemi” on composite films based on gelatin. Food Hydrocolloids, 19(1), 73–82.Byun, Y., Kim, Y. T., & Whiteside, S. (2010). Characterization of an antioxidant polylactic acid (PLA) film prepared with alpha-tocopherol, BHT and polyethylene glycol using film cast extruder. Journal of Food Engineering, 100, 239–244.Cerqueira, M. A., Costa, M. J., Fuciños, C., Pastrana, L. M., & Vicente, A. A. (2014). Development of active and nanotechnology-based smart edible packaging systems: physical-chemical characterization. Food and Bioprocess Technology, 7(5), 1472–1482.Choi, W. S., & Han, J. H. (2001). Physical and mechanical properties of pea–protein-based edible films. Journal of Food Science, 66, 319–322.Choi, W. S., & Han, J. H. (2002). Film-forming mechanism and heat denaturation effects on the physical and chemical properties of pea-protein-isolate edible films. Journal of Food Science, 67, 1399–1406.Fabra, M. J., Talens, P., & Chiralt, A. (2009). Microstructure and optical properties of sodium caseinate films containing oleic acidebeeswax mixtures. Food Hydrocolloids, 23, 676–683.Fabra, M. J., Talens, P., & Chiralt, A. (2010). Water sorption isotherms and phase transitions of sodium caseinate–lipid films as affected by lipid interactions. Food Hydrocolloids, 24, 384–391.Fabra, M. J., Hambleton, A., Talens, P., Debeaufort, F., & Chiralt, A. (2011). Effect of ferulic acid and α-tocopherol antioxidants on properties of sodium caseinate edible films. Food Hydrocolloids, 25, 1441–1447.Fabra, M. J., Talens, P., Gavara, R., & Chiralt, A. (2012). Barrier properties of sodium caseinate films as affected by lipid composition and moisture content. Journal of Food Engineering, 109, 372–379.Frankel, E. N., Huang, S. W., Kanner, J., & German, J. B. (1994). Interfacial phenomena in the evaluation of antioxidants: bulk oils vs emulsions. Journal of Agriculture and Food Chemistry, 42(5), 1054–1059.Gómez-Estaca, J., Giménez, B., Montero, P., & Gómez-Guillén, M. C. (2009). Incorporation of antioxidant borage extract into edible films based on sole skin gelatin or a commercial fish gelatin. Journal of Food Engineering, 92, 78–85.Huang, S. W., Frankel, E. N., & German, J. B. (1994). Antioxidant activity of alpha.- and.gamma.-tocopherols in bulk oils and in oil-in-water emulsions. Journal of Agriculture and Food Chemistry, 42(10), 2108–2114.Hutchings, J. B. (1999). Food and colour appearance (2nd ed.). Gaithersburg: Chapman and Hall Food Science Book, Aspen Publication.Jiménez, A., Fabra, M. J., Talens, P., & Chiralt, A. (2010). Effect of lipid self-association on the microstructure and physical properties of hydroxypropylmethylcellulose edible films containing fatty acids. Carbohydrate Polymers, 82(3), 585–593.Jiménez, A., Fabra, M. J., Talens, P., & Chiralt, A. (2013). Physical properties and antioxidant capacity of starch-sodium caseinate films containing lipids. Journal of Food Engineering, 116(3), 695–702.Jung, M. Y., & Min, D. B. (1990). Effects of alpha-. γ-, and δ-tocopherols on oxidative stability of soybean oil. Journal of Food Science, 55(5), 1464–1465.López-de-Dicastillo, C., Alonso, J. M., Catalá, R., Gavara, R., & Hernández-Muñoz, P. (2010). Improving the antioxidant protection of packaged food by incorporating natural flavonoids into ethylene-vinyl alcohol copolymer (EVOH) films. Journal of Agricultural and Food Chemistry, 58, 10958–10964.Ma, W., Tang, C.-H., Yin, S.-W., Yang, X. Q., Qi, J. R., & Xia, N. (2012). Effect of homogenization conditions on properties of gelatin-olive oil composite films. Journal of Food Engineering, 113(1), 136–142.Mauer, L. J., Smith, D. E., & Labuza, T. P. (2000). Water vapor permeability, mechanical, and structural properties of edible β-casein films. International Dairy Journal, 10(5–6), 353–358.Mc Hugh, T. H., Avena-Bustillos, R., & Krochta, J. M. (1993). Hydrophobic edible films:modified procedure for water vapor permeability and explanation of thickness effects. Journal of Food Science, 58(4), 899–903.McHugh, T. H., & Krochta, J. M. (1994). Dispersed phase particle size effects on water vapour permeability of whey protein–beeswax emulsion films. Journal of Food Processing and Preservation, 18, 173–188.Ozkan, G., Simsek, B., & Kuleasan, H. (2007). Antioxidant activities of Satureja cilicica essential oil in butter and in vitro. Journal of Food Engineering, 79, 1391–1396.Pereira de Abreu, D. A., Paseiro Losada, P., Maroto, J., & Cruz, J. M. (2011). Natural antioxidant active packaging film and its effect on lipid damage in frozen blue shark (Prionace glauca). Innovative Food Science and Emerging Technologies, 12, 50–55.Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decoloration assay. Free Radical Biology and Medicine, 26, 1231–1237.Roos, Y. H. (1995). Phase transitions in food. San Diego: Academic Press.Salgado, P. R., Molina Ortiz, S. E., Petruccelli, S., & Mauri, A. N. (2010). Biodegradable sunflower protein films naturally activated with antioxidant compounds. Food Hydrocolloids, 24(5), 525–533.Salgado, P. R., Fernández, G. B., Drago, S. R., & Mauri, A. N. (2011). Addition of bovine plasma hydrolysates improves the antioxidant properties of soybean and sunflower protein-based films. Food Hydrocolloids, 25, 1433–1440.Samaranayaka, A. G. P., & Li-Chan, E. C. Y. (2008). Autolysis-assisted production of fish protein hydrolysates with antioxidant properties form Pacific hake (Merluccius productus). Food Chemistry, 107, 768–776.Souza, B. W. S., Cerqueira, A., Casariego, A., Lima, A. M. P., Teixeira, J. A., & Vicente, A. A. (2009). Effect of moderate electric fields in the permeation properties of chitosan coatings. Food Hydrocolloids, 23, 2110–2115

Physical and Antimicrobial Properties of Compression-Molded Cassava Starch-Chitosan Films for Meat Preservation

[EN] Cassava starch-chitosan films were obtained by melt bending and compression molding, using glycerol and polyethylene glycol as plasticizers. Both the starch/chitosan and the polymer/plasticizer ratios were varied in order to analyze their effect on the physical properties of the films. Additionally, the antimicrobial activity of 70:30 polymer:plasticizer films was tested in cold-stored pork meat slices as affected by chitosan content. All film components were thermally stable up to 200 A degrees C, which guaranteed their thermostability during film processing. Starch and chitosan had limited miscibility by melt blending, which resulted in heterogeneous film microstructure. Polyethylene glycol partially crystallized in the films, to a greater extent as the chitosan ratio increased, which limited its plasticizing effect. The films with the highest plasticizer ratio were more permeable to water vapor, less rigid, and less resistant to break. The variation in the chitosan content did not have a significant effect on water vapor permeability. As the chitosan proportion increased, the films became less stretchable, more rigid, and more resistant to break, with a more saturated yellowish color. The incorporation of the highest amount of chitosan in the films led to the reduction in coliforms and total aerobic counts of cold-stored pork meat slices, thus extending their shelf-life.The authors acknowledge the financial support provided by the Spanish Ministerio de Economia y Competividad (Projects AGL2013-42989-R and AGL2016-76699-R). Author Cristina Valencia-Sullca thanks the Peruvian Grant National Program (PRONABEC Grant).Valencia-Sullca, CE.; Atarés Huerta, LM.; Vargas, M.; Chiralt, A. (2018). Physical and Antimicrobial Properties of Compression-Molded Cassava Starch-Chitosan Films for Meat Preservation. Food and Bioprocess Technology. 11(7):1339-1349. https://doi.org/10.1007/s11947-018-2094-5S13391349117Alves, V. 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