Anomalous magnetophotoluminescence as a result of level repulsion in arrays of quantum dots

Selectively excited photoluminescence (SPL) of an array of self-organized In$_{0.5}$Ga$_{0.5}$As quantum dots has been measured in a magnetic field up to 11T. Anomalous magnetic field sensitivity of the SPL spectra has been observed under conditions for which the regular photoluminescence spectra is insensitive to the magnetic field due to large inhomogeneous broadening. The anomalous sensitivity is interpreted in terms of the repulsion of excited levels of the dots in a random potential. A theory presented to describe this phenomena is in excellent agreement with the experimental data. The data estimated the correlation in the positions of excited levels of the dots to be 94%. The magnetic field dependence allows the determination of the reduced cyclotron effective mass in a dot. For our sample we have obtained $m_em_h/(m_e+m_h)=0.034m_0$.Comment: 12 revtex preprint pages + 4 ps figures, uuencode

Expression of chicken hepatic type I and type III iodothyronine deiodinases during embryonic development

In embryonic chicken liver (ECL) two types of iodothyronine deiodinases are expressed: D1 and D3. D1 catalyzes the activation as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. D3 only catalyzes inner ring deiodination. D1 and D3 have been cloned from mammals and amphibians and shown to contain a selenocysteine (Sec) residue. We characterized chicken D1 and D3 complementary DNAs (cDNAs) and studied the expression of hepatic D1 and D3 messenger RNAs (mRNAs) during embryonic development. Oligonucleotides based on two amino acid sequences strongly conserved in the different deiodinases (NFGSCTSecP and YIEEAH) were used for reverse transcription-PCR of poly(A+) RNA isolated from embryonic day 17 (E17) chicken liver, resulting in the amplification of two 117-bp DNA fragments. Screening of an E17 chicken liver cDNA library with these probes led to the isolation of two cDNA clones, ECL1711 and ECL1715. The ECL1711 clone was 1360 bp long and lacked a translation start site. Sequence alignment showed that it shared highest sequence identity with D1s from other vertebrates and that the coding sequence probably lacked the first five nucleotides. An ATG start codon was engineered by site-directed mutagenesis, generating a mutant (ECL1711M) with four additional codons (coding for MGTR). The open reading frame of ECL1711M coded for a 249-amino acid protein showing 58-62% identity with mammalian D1s. An in-frame TGA codon was located at position 127, which is translated as Sec in the presence ofa Sec insertion sequence (SECIS) identified in the 3'-untranslated region. Enzyme activity expressed in COS-1 cells by transfection with ECL1711M showed the same catalytic, substrate, and inhibitor specificities as native chicken D1. The ECL1715 clone was 1366 bp long and also lacked a translation start site. Sequence alignment showed that it was most homologous with D3 from other species and that the coding sequence lacked approximately the first 46 nucleotides. The deduced amino acid sequence showed 62-72% identity with the D3 sequences from other species, including a putative Sec residue at a corresponding position. The 3'-untranslated region of ECL1715 also contained a SECIS element. These results indicate that ECL1711 and ECL1715 are near-full-length cDNA clones for chicken D1 and D3 selenoproteins, respectively. The ontogeny of D1 and D3 expression in chicken liver was studied between E14 and 1 day after hatching (C1). D1 activity showed a gradual increase from E14 until C1, whereas D1 mRNA level remained relatively constant. D3 activity and mRNA level were highly significantly correlated, showing an increase from E14 to E17 and a strong decrease thereafter. These results suggest that the regulation of chicken hepatic D3 expression during embryonic development occurs predominantly at the pretranslational level

Simulating progressive motor neuron degeneration and collateral reinnervation in motor neuron diseases using a dynamic muscle model based on human single motor unit recordings

Objective.To simulate progressive motor neuron loss and collateral reinnervation in motor neuron diseases (MNDs) by developing a dynamic muscle model based on human single motor unit (MU) surface-electromyography (EMG) recordings.Approach.Single MU potentials recorded with high-density surface-EMG from thenar muscles formed the basic building blocks of the model. From the baseline MU pool innervating a muscle, progressive MU loss was simulated by removal of MUs, one-by-one. These removed MUs underwent collateral reinnervation with scenarios varying from 0% to 100%. These scenarios were based on a geometric variable, reflecting the overlap in MU territories using the spatiotemporal profiles of single MUs and a variable reflecting the efficacy of the reinnervation process. For validation, we tailored the model to generate compound muscle action potential (CMAP) scans, which is a promising surface-EMG method for monitoring MND patients. Selected scenarios for reinnervation that matched observed MU enlargements were used to validate the model by comparing markers (including the maximum CMAP and a motor unit number estimate (MUNE)) derived from simulated and recorded CMAP scans in a cohort of 49 MND patients and 22 age-matched healthy controls.Main results.The maximum CMAP at baseline was 8.3 mV (5th-95th percentile: 4.6 mV-11.8 mV). Phase cancellation caused an amplitude drop of 38.9% (5th-95th percentile, 33.0%-45.7%). To match observations, the geometric variable had to be set at 40% and the efficacy variable at 60%-70%. The Δ maximum CMAP between recorded and simulated CMAP scans as a function of fitted MUNE was -0.4 mV (5th-95th percentile = -4.0 - +2.4 mV).Significance.The dynamic muscle model could be used as a platform to train personnel in applying surface-EMG methods prior to their use in clinical care and trials. Moreover, the model may pave the way to compare biomarkers more efficiently, without directly posing unnecessary burden on patients.</p

Characterization of a propylthiouracil-insensitive type I iodothyronine deiodinase

Mammalian type I iodothyronine deiodinase (D1) activates and inactivates thyroid hormone by outer ring deiodination (ORD) and inner ring deiodination (IRD), respectively, and is potently inhibited by propylthiouracil (PTU). Here we describe the cloning and characterization of a complementary DNA encoding a PTU-insensitive D1 from teleost fish (Oreochromis niloticus, tilapia). This complementary DNA codes for a protein of 248 amino acids, including a putative selenocysteine (Sec) residue, encoded by a TGA triplet, at position 126. The 3' untranslated region contains two putative Sec insertion sequence (SECIS) elements. Recombinant enzyme expressed in COS-1 cells catalyzes both ORD of T4 and rT3 and IRD of T3 and T3 sulfate with the same substrate specificity as native tilapia D1 (tD1), i.e. rT3 >> T4 > T3 sulfate > T3. Native and recombinant tD1 show equally low sensitivities to inhibition by PTU, iodoacetate, and gold thioglucose compared with the potent inhibitions observed with mammalian D1s. Because the residue 2 positions downstream from Sec is Pro in tD1 and in all (PTU-insensitive) type II and type III iodothyronine deiodinases but Ser in all PTU-sensitive D1s, we prepared the Pro128Ser mutant of tD1. The mutant enzyme showed strongly decreased ORD and somewhat increased IRD activity, but was still insensitive to PTU. These results provide new information about the structure-activity relationship of D1 concerning two characteristic properties, i.e. catalysis of both ORD and IRD, and inhibition by PTU

Low-energy p-d Scattering: High Precision Data, Comparisons with Theory, and Phase-Shift Analyses

Angular distributions of sigma(theta), A_y, iT_11, T_20, T_21, and T_22 have been measured for d-p scattering at E_c.m.=667 keV. This set of high-precision data is compared to variational calculations with the nucleon-nucleon potential alone and also to calculations including a three-nucleon (3N) potential. Agreement with cross-section and tensor analyzing power data is excellent when a 3N potential is used. However, a comparison between the vector analyzing powers reveals differences of approximately 40% in the maxima of the angular distributions which is larger than reported at higher energies for both p-d and n-d scattering. Single-energy phase-shift analyses were performed on this data set and a similar data set at E_c.m.=431.3 keV. The role of the different phase-shift parameters in fitting these data is discussed.Comment: 18 pages, 6 figure

Radiative multipole moments of integer-spin fields in curved spacetime

Radiative multipole moments of scalar, electromagnetic, and linearized gravitational fields in Schwarzschild spacetime are computed to third order in v in a weak-field, slow-motion approximation, where v is a characteristic velocity associated with the motion of the source. To zeroth order in v, a radiative moment of order l is given by the corresponding source moment differentiated l times with respect to retarded time. At second order in v, additional terms appear inside the spatial integrals. These are near-zone corrections which depend on the detailed behavior of the source. At third order in v, the correction terms occur outside the spatial integrals, so that they do not depend on the detailed behavior of the source. These are wave-propagation corrections which are heuristically understood as arising from the scattering of the radiation by the spacetime curvature surrounding the source. Our calculations show that the wave-propagation corrections take a universal form which is independent of multipole order and field type. We also show that in general relativity, temporal and spatial curvatures contribute equally to the wave-propagation corrections.Comment: 34 pages, ReVTe

phosphorus and potassium fertilizer effects on alfalfa and soil in a non limited soil

Fertilization strategies for high-yielding alfalfa (Medicago sativa L.) should take in account the increase in soil nutritional status that occurred during the last decades in areas with intensive agricultural use. A field study was conducted at the University of Padova, northeastern Italy, to determine the response of alfalfa yield and nutritive value to various combinations of P and K rates in a soil lacking nutrient deficiency. Alfalfa cultivar Delta was seeded in March 2005 on a silt loam soil having 38 mg kg -1 available P and 178 mg kg -1 exchangeable K. Nine treatments deriving from the combination of three P fertilization rates (0, 100, and 200 kg ha -1 P 2 O 5 ) and three K rates (0, 300, and 600 kg ha -1 K 2 O) were compared in a randomized complete block design. Plots were harvested at bud stage during three growing seasons (2005-2007) and dry matter (DM) yield, forage nutritive value, P and K contents, canopy height, and stem density were measured at each harvest. Soil samples were collected at the end of the research period for determination of available P and exchangeable K. The results demonstrated that P application had no impact on yield and did not interact with K in determining productivity, while K had a positive effect on yield. However, the 300 kg ha -1 K 2 O rate appeared sufficient to maximize yield, without adverse effects on the forage nutritive value. Data from soil analyses showed that alfalfa has a high K uptake even when it is fertilized at high rates

Constraining primordial non-Gaussianity with cosmological weak lensing: shear and flexion

We examine the cosmological constraining power of future large-scale weak lensing surveys on the model of \emph{Euclid}, with particular reference to primordial non-Gaussianity. Our analysis considers several different estimators of the projected matter power spectrum, based on both shear and flexion, for which we review the covariances and Fisher matrices. The bounds provided by cosmic shear alone for the local bispectrum shape, marginalized over $\sigma_8$, are at the level of $\Delta f_\mathrm{NL} \sim 100$. We consider three additional bispectrum shapes, for which the cosmic shear constraints range from $\Delta f_\mathrm{NL}\sim 340$ (equilateral shape) up to $\Delta f_\mathrm{NL}\sim 500$ (orthogonal shape). The competitiveness of cosmic flexion constraints against cosmic shear ones depends on the galaxy intrinsic flexion noise, that is still virtually unconstrained. Adopting the very high value that has been occasionally used in the literature results in the flexion contribution being basically negligible with respect to the shear one, and for realistic configurations the former does not improve significantly the constraining power of the latter. Since the flexion noise decreases with decreasing scale, by extending the analysis up to $\ell_\mathrm{max} = 20,000$ cosmic flexion, while being still subdominant, improves the shear constraints by $\sim 10$ when added. However on such small scales the highly non-linear clustering of matter and the impact of baryonic physics make any error estimation uncertain. By considering lower, and possibly more realistic, values of the flexion intrinsic shape noise results in flexion constraining power being a factor of $\sim 2$ better than that of shear, and the bounds on $\sigma_8$ and $f_\mathrm{NL}$ being improved by a factor of $\sim 3$ upon their combination. (abridged)Comment: 30 pages, 4 figures, 4 tables. To appear on JCA