Bacteria isolated from milk of dairy cows with and without clinical mastitis in different regions of Australia and their AMR profiles

Mastitis is the most common disease in dairy cattle worldwide. The objectives of this study were to estimate the prevalence of different bacterial species associated with mastitis from dairy herds located in geographically and climatically distinct zones in Australia, and to evaluate the antimicrobial susceptibility of the isolated bacteria. Quarter-level milk samples (n = 419) were collected from 151 mastitis cases and 268 healthy controls originating from 18 dairy herds located in tropical (Northern Queensland), subtropical (Southeast Queensland) and temperate zones (Victoria) between March and June 2019. Milk samples were cultured, and the isolated bacteria were grouped into six groups: Enterobacteriaceae spp.; Streptococcus spp.; Staphylococcus aureus, non-aureus staphylococci (NAS); Bacillus spp.; and Others. Mixed effects conditional logistic regression models were applied to quantify the association between the prevalence of each bacterial group and the herd zone and bulk milk tank somatic cell counts (BMTSCC). Of the 205 isolates, 102 (50%) originated from mastitis cases, and 103 (50%) from controls. Staphylococci were the most prevalent (NAS 32% and S. aureus 11%). Contagious mastitis bacteria were more prevalent in Victoria compared to Queensland dairy herds. NAS species (P 300,000 cells/mL compared with herds with low BMTSCC ≤150,000 cells/mL. Enterobacteriaceae and Streptococcus spp. groups showed high resistance rates to 1 (51 and 47%, respectively), and 2 (11 and 23%, respectively), antimicrobials. More than one third of the Enterobacteriaceae (48%) and Others (43%) groups spp. were resistant to at least three antimicrobials. This study provided a unique opportunity to investigate the prevalence of mastitis-associated bacteria in clinical cases and in apparently healthy controls. The findings of this study help inform mastitis control and antimicrobial stewardship programs aimed to reduce the prevalence of mastitis and antimicrobial resistance in dairy herds

Systematic review and meta-analysis of probiotic use on inflammatory biomarkers and disease prevention in cattle

The aim of this study was to appraise the available evidence on the effectiveness of probiotic treatment on mature cattle immunity, inflammation, and disease prevention. A systematic review with meta-analysis was conducted to analyse studies that were eligible to answer the following research question: “in cattle of at least 6-months of age, is the use of probiotics associated with immunomodulatory and inflammatory responses, and clinical disease outcomes?” Our literature search yielded 25 studies that fit the inclusion criteria. From these studies, only 19 were suitable for inclusion in the meta-analysis due to data limitations and differences in study population characteristics. Included studies were assessed for bias using a risk assessment tool adapted from the Cochrane Collaboration’s tool for assessing risk of bias in randomised trials. GRADE guidelines were used to assess the quality of the body of evidence at the outcome level. The meta-analysis was performed using Review Manager and R. The overall quality of evidence at the outcome level was assessed as being very low. On average, the treatment effect on immunoglobulin G (IgG), serum amyloid A (SAA), haptoglobin (Hp) and β-hydroxybutyrate (BoHB) for cows receiving probiotics did not differ from control cows. Exposure to probiotics was not associated with reduced risk of reproductive disorders (pooled RR = 1.02 95 % CI = 0.81–1.27, P = 0.88). There is insufficient evidence to support any significant positive effects of probiotics on cattle immunity and disease prevention. This lack of consistent evidence could be due to dissimilarities in the design of the included studies such as differences in dosage, dose schedule, diet composition and/or physiological state of the host at the time of treatment

The effect of clinical lameness on liveweight in a seasonally calving, pasture-fed dairy herd

This study investigated the effect of lameness on liveweight (LW) in pasture-fed dairy cattle. The data comprised 222,446 averaged daily LW measurements from 828 lactations of 542 mixed-age cows in a seasonally calving, pasture-fed New Zealand dairy herd. The LW measurements for individual cows were aggregated into weekly averages and analyses conducted to evaluate the effect of a diagnosis of lameness on LW change after controlling for the effect of week in milk, parity, LW at calving, breed, calendar month, and season. In lame cows, LW decreased for up to 3. wk before lameness was diagnosed and for up to 4. wk after treatment. Total LW loss arising from a single lameness episode was, on average, 61. kg (95% confidence interval: 47 to 74. kg). The results from this study demonstrate how LW records for individual animals can be used to enhance a herd manager's ability to detect lame cows and present them for treatment. The methods presented here show how daily LW monitoring might be used as a tool for early detection of lameness in dairy cattle

The effect of lameness on the fertility of dairy cattle in a seasonally breeding pasture-based system

The effect of lameness on the fertility of dairy cattle is well recognized. But, the effect of lameness on the fertility of seasonally breeding cattle in pasture-based systems is less well characterized. This prospective cohort study of 463 cows on 1 farm in the lower North Island of New Zealand was designed to assess the effect of clinical lameness, as identified by farm staff, on the hazard of conception after the planned start-of-mating date. A Cox proportional hazards model with time-varying covariates was used. After controlling for the effect of parity, breed, body weight at calving, and calving-to-planned start of mating interval, the daily hazard of conception for cows identified as lame was 0.78 (95% confidence interval: 0.68-0.86) compared with non-lame cows. Lame cows took 12. d longer to get pregnant compared with their non-lame counterparts