2,328 research outputs found
Skills Obsolescence: Causes and Cures
Until now there has been surprisingly little research on the causes of and the remedies for skills obsolescence. This study tries to fill that gap to some extent by analysing the relation between risk factors and skills obsolescence. Moreover, the role remedies play to counter skills obsolescence is analysed. Four empirical analyses that relate skills obsolescence to risk factors and remedies are presented. We find that most risk factors identified in the literature can be validated empirically. The remedies for skills obsolescence are not effective in all situations: the results show that there is considerable variation in the effectiveness of the remedies across different types of skills obsolescence. Although current available data does not allow a comprehensive analysis, which also takes account of relations between the various types of skills obsolescence, the results obtained are plausible and offer a starting point for further research.education, training and the labour market;
Molecular biological methods for studying the gut microbiota : the EU human gut flora project
Seven European laboratories co-operated in a joint project (FAIR CT97-3035) to develop, refine and apply molecular methods towards facilitating elucidation of the complex composition of the human intestinal microflora and to devise robust methodologies for monitoring the gut flora in response to diet. An extensive database of 16S rRNA sequences for tracking intestinal bacteria was generated by sequencing the 16S rRNA genes of new faecal isolates and of clones obtained by amplification with polymerase chain reaction (PCR) on faecal DNA from subjects belonging to different age groups. The analyses indicated that the number of different species (diversity) present in the human gut increased with age. The sequence information generated, provided the basis for design of 16S rRNA-directed oligonucleotide probes to specifically detect bacteria at various levels of phylogenetic hierarchy. The probes were tested for their specificity and used in whole-cell and dot-blot hybridisations. The applicability of the developed methods was demonstrated in several studies and the major outcomes are described
Direct measurement of the radiative lifetime of vibrationally excited OH radicals
Neutral molecules, isolated in the gas-phase, can be prepared in a long-lived
excited state and stored in a trap. The long observation time afforded by the
trap can then be exploited to measure the radiative lifetime of this state by
monitoring the temporal decay of the population in the trap. This method is
demonstrated here and used to benchmark the Einstein -coefficients in the
Meinel system of OH. A pulsed beam of vibrationally excited OH radicals is
Stark decelerated and loaded into an electrostatic quadrupole trap. The
radiative lifetime of the upper -doublet component of the level is determined as ms, in good
agreement with the calculated value of ms.Comment: 4 pages, 3 figures, submitted to Phys. Rev. Let
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