Efecto del porcentaje de inclusión de lisina sobre la productividad en cerdos de cebo

Las necesidades en lisina (Lys) de cerdos de altos crecimientos sacrificados para venta en fresco han sido estudiadas en detalle (NRC, 1998; BSAS, 2003; Fedna, 2006). Sin embargo, las necesidades de cerdos destinados a la obtención de productos curados, en los que se precisa obtener canales con porcentajes de grasa óptimos, han sido poco estudiadas (Beaulie et al., 2009). A ello hay que sumarle que en los últimos años la genética se ha centrado en mejorar la eficiencia alimenticia, lo que en muchos casos penaliza el contenido graso de las partes nobles (Blanchard et al., 1999; Latorre et al., 2003). Conocer las necesidades en Lys de cerdos de alta producción es clave; un déficit de Lys conlleva un menor crecimiento mientras que el exceso es caro. Por ello, el objetivo de este estudio fue estudiar el efecto del nivel de inclusión de Lys sobre los rendimientos productivos en cerdos de alta selección sacrificados a 113 kg destinados a la producción de productos curados

Laminin and integrin expression in the ventral ectodermal ridge of the mouse embryo: Implications for regulation of BMP signalling

PMCID: PMC3629792.[Background]: The ventral ectodermal ridge (VER) is an important signalling centre in the mouse tail-bud following completion of gastrulation. BMP regulation is essential for VER function, but how these signals are transmitted between adjacent tissues is unclear. [Results]: We investigated the idea that extracellular matrix components might be involved, using immunohistochemistry and in situ hybridisation to detect all known α, β, and γ laminin chains and their mRNAs in the early tail bud. We identified an apparently novel laminin variant, comprising α5, β3 and γ2 chains, as a major component of the VER basement membrane at E9.5. Strikingly, only the mRNAs for these chains were co-expressed in VER cells, suggesting that lamin532 may be the sole basement membrane laminin at this stage. Since α6 integrin was also expressed in VER cells, this raises the possibility of cell-matrix interactions regulating BMP signalling at this site of caudal morphogenesis. [Conclusions]: Laminin532 could interact with α6-containing integrin to direct differentiation of the specialised VER cells from surface ectoderm.This work was supported by de Instituto de Salud Carlos III project CP08/00111 and PS09/00050 (to P.Y.-G.), la Consejería de Salud de la Junta de Andalucia project PI-0438-2010 (to P.Y.-G.), and the Wellcome Trust (to A.J.C.).Peer reviewe

The potential role of the adipokine HMGB1 in obesity and insulin resistance. Novel effects on adipose tissue biology

Discovery of the adipose tissue as a major source of signaling molecules almost three decades ago set a novel physiological paradigm that paved the way for the identification of metabolic organs as endocrine organs. Adipocytes, the main adipose tissue cell type, do not only represent the principal site of energy storage in form of triglycerides, but also produce a variety of molecules for short and long distance intercellular communication, named adipokines, which coordinate systemic responses. Although the best known adipokines identified and characterized hitherto are leptin and adiponectin, novel adipokines are continuously being described, what have significantly helped to elucidate the role of adipocyte biology in obesity and associated comorbidities. One of these novel adipokines is high-mobility group box 1 (HMGB1), a ubiquitous nuclear protein that has been recently reported to be dysregulated in obese dysfunctional adipocytes. Although the classical function of HMGB1 is related to inflammation and immunity, acting as an alarmin, novel advances evidence an active implication of HMGB1 in tissue remodeling and fibrosis. This review summarizes the current evidence on the mechanisms controlling HMGB1 release, as well as its role as a regulator of adipocyte function and extracellular matrix remodeling, with special emphasis on the potential of this novel adipokine as a target in the obesity treatment

Effect of highly dispersed yttria addition on thermal stability of hydroxyapatite

The capability of the colloidal method to produce yttria (Y₂O₃) dispersed hydroxyapatite (HA) has been investigated as an alternative method to the conventional method of mechanical mixing and sintering for developing HA-based materials that could exhibit controllable and enhanced functional properties. A water based colloidal route to produce HA materials with highly dispersed Y₂O₃ has been applied, and the effect of 10 wt.% Y₂O₃ addition to HA investigated by thermal analysis, X-ray diffraction and Fourier transform infrared spectroscopy. These measurements evidence a remarkable effect of this Y₂O₃ addition on decomposition mechanisms of synthetic HA. Results show that incorporation of Y₂O₃ as dispersed second phase is beneficial because it hinders the decomposition mechanisms of HA into calcium phosphates. This retardation will allow the control of the sintering conditions for developing HA implants with improved properties. Besides, substitution of Ca⁻2+ with Y⁻3+ ions appears to promote the formation of OH- vacancies, which could improve the conductive properties of HA favorable to osseointegration.This work has been supported by the Ministry of Science and Innova tion of Spain (MICINN) under contracts MAT2009 14448 C02 01 and IPT 310000 2010 12, and Regional Government of Madrid through the ESTRUMAT CM program (MAT 1585).Publicad

Microstructural characterization of alumina-zirconia layered ceramics using positron annihilation spectroscopy

Positron annihilation spectroscopy (PAS), indentation, nanoindentation experiments and scanning electron microscopy (SEM) observations were performed on Al₂O₃-ZrO₂ laminates samples to assess the effect of residual stresses on their mechanical and microstructural properties. Layered samples were implemented by slip-casting, constituted by two thin Al₂O₃ external layers and an intermediate thick one, consisting of a mixture of Al₂O₃ and monoclinic ZrO₂ in the range 0-30 vol.%. In these systems residual tensile stresses fields were generated inside the external layers during cooling from the sintering temperature, by the expansion of the adjacent ZrO₂-containing layer. SEM observations showed the microstructural effects due to the level of tension related to the zirconia content. A correlation between the PAS parameters and the microstructural changes caused by the presence of residual stresses was found. Nanoindentation measurements were used to trace the sign and magnitude of the residual stress gradient across the interface between the layers.This work was supported by Spanish Government under Contract MAT2006-01038. The authors gratefully acknowledge the financial support from the Comunidad de Madrid and the Ministry of Education and Science of Spain, through the ESTRUMAT-CM (MAT/77) programs.Publicad

Reduction of Die Wear and Structural Defects of Railway Screw Spike Heads Estimated by FEM

Railway spike screws are manufactured by hot forging on a massive scale, due to each kilometer of railway track needing 8600 spike screws. These components have a low market value, so the head must be formed in a single die stroke. The service life of the dies is directly related to the amount of energy required to form a single screw. The existing standard for spike screws specifies only the required tolerances for the head dimensions, particularly the angle of the hub faces and the radius of agreement of the hub with the cap. Both geometrical variables of the head and process conditions (as-received material diameter and flash thickness) are critical parameters in spike production. This work focuses on minimizing the energy required for forming the head of a railway spike screw by computational simulation. The variables with the highest degree of incidence on the energy, forging load, and filling of the die are ordered statistically. The results show that flash thickness is the variable with the most significant influence on forming energy and forming load, as well as on die filling. Specifically, the minimum forming energy was obtained for combining of a hub wall angle of 1.3° an as-received material diameter of 23.54 mm and a flash thickness of 2.25 mm. Flash thickness generates a lack of filling at the top vertices of the hub, although this defect does not affect the functionality of the part or its serviceability. Finally, the wear is mainly concentrated on the die splice radii, where the highest contact pressure is concentrated according to the computational simulation results.This work is supported by the Serra Húnter program (Generalitat de Catalunya) reference number (UPC-LE-304 (2018)) and by the Aeronautics Advanced Manufacturing Center (CFAA). Thanks also are given to special agreement INTI-Faculty of engineering of Bilbao and to university group grant IT 1337-19

Solvent-assisted in situ synthesis of cysteamine-capped silver nanoparticles

Silver nanoparticles offer a huge potential for biomedical applications owing to their exceptional properties and small size. Specifically, cysteamine-capped silver nanoparticles could form the basis for new anticancer therapies combining the cytotoxic effect of the silver core with the inherent antitumor activity of cysteamine, which inhibit cancer cell proliferation and suppress invasion and metastasis. In addition, the capability of the cysteamine coating monolayer to couple a variety of active principles and targeting (bio)molecules of interest proves key to the tailoring of this platform in order to exploit the pathophysiology of specific tumor types. Nevertheless, the chain length and conformational flexibility of cysteamine, together with its ability to attach to the surface of silver nanoparticles via both the thiol and the amine group, have made the in situ synthesis of these particles an especially challenging task. Herein we report a solvent-assisted in situ synthesis method that solves this problem. The obtained nanoparticles have been fully characterized by UV–visible absorption spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, electron diffraction measurement, high resolution transmission electron microscopy, scanning transmission electron microscopy, energy dispersive x-ray spectroscopy nanoanalysis, and dynamic light scattering measurement. Our synthesis method achieves extremely high yield and surface coating ratio, and colloidal stability over a wide range of pH values including physiological pH. Additionally, we have demonstrated that cysteamine-capped nanoparticles obtained by this method can be conjugated to an antibody for active targeting of the epidermal growth factor receptor, which plays an important role in the pathogenesis and progression of a wide variety of tumors, and induce cell death in human squamous carcinoma cells. We believe this method can be readily extended to combinations of noble metals and longer chain primary, secondary, ternary or even quaternary aminethiolsEspaña, Junta de Andalucía P10- FQM-6615España, Ministerio de Economia y Competitividad CTP2016-80206-

Apoptotic microtubules delimit an active caspase free area in the cellular cortex during the execution phase of apoptosis

Apoptotic microtubule network (AMN) is organized during apoptosis, forming a cortical structure beneath plasma membrane, which has an important role in preserving cell morphology and plasma membrane permeability. The aim of this study was to examine the role of AMN in maintaining plasma membrane integrity during the execution phase of apoptosis. We demonstrated in camptothecin-induced apoptosis in H460 cells that AMN delimits an active caspase free area beneath plasma membrane that permits the preservation of cellular cortex and transmembrane proteins. AMN depolymerization in apoptotic cells by a short exposure to colchicine allowed active caspases to reach the cellular cortex and cleave many key proteins involved in plasma membrane structural support, cell adhesion and ionic homeostasis. Cleavage of cellular cortex and plasma membrane proteins, such as a-spectrin, paxilin, focal adhesion kinase (FAK), E-cadherin and integrin subunit b4 was associated with cell collapse and cell detachment. Otherwise, cleavage-mediated inactivation of calcium ATPase pump (PMCA-4) and Naþ/Ca2þ exchanger (NCX) involved in cell calcium extrusion resulted in calcium overload. Furthermore, cleavage of Naþ/Kþ pump subunit b was associated with altered sodium homeostasis. Cleavage of cell cortex and plasma membrane proteins in apoptotic cells after AMN depolymerization increased plasma permeability, ionic imbalance and bioenergetic collapse, leading apoptotic cells to secondary necrosis. The essential role of caspase-mediated cleavage in this process was demonstrated because the concomitant addition of colchicine that induces AMN depolymerization and the pan-caspase inhibitor z-VAD avoided the cleavage of cortical and plasma membrane proteins and prevented apoptotic cells to undergo secondary necrosis. Furthermore, the presence of AMN was also critical for proper phosphatidylserine externalization and apoptotic cell clearance by macrophages. These results indicate that AMN is essential to preserve an active caspase free area in the cellular cortex of apoptotic cells that allows plasma membrane integrity during the execution phase of apoptosis

Practices in Vehicle Engineering: MATLAB programming for processing experimental data in tire tests

[ES] En este trabajo, se pretende involucrar al estudiante en el proceso de medición y procesamiento de los datos en las prácticas de laboratorio de neumáticos en la asignatura de ingeniería de vehículos. El procesamiento de los datos, debido a su complejidad suele omitirse, sin embargo, esta propuesta enseña a los estudiantes a tratar con el ruido de los sensores, el alcance y la precisión de cada medición. Partiendo de una plantilla MATLAB diseñaran métodos de filtrado y procesamiento de datos.[EN] The aim of this work is to involve the student in the measurement and data processing of tire laboratory experiments in the course of vehicle engineering. Data processing, due to its complexity, is often omitted, however, this proposal teaches students to deal with sensor noise, range, and accuracy of each measurement. By using a MATLAB template, they will design data filtering and processing methods.Pérez Fernández, J.; Alcázar Vargas, M.; Sánchez Andrades, I.; Carabias Acosta, E.; Castillo Aguilar, JJ. (2023). Prácticas en Ingeniería de Vehículos: Programación en MATLAB del procesado de datos experimentales en ensayos de neumáticos. Modelling in Science Education and Learning. 16(2):13-19. https://doi.org/10.4995/msel.2023.19078131916