Halothane hepatitis with renal failure treated with hemodialysis and exchange transfusion

A 38-year-old white female, hepatitis B antigen negative, developed fluminating hepatic failure associated with oliguria and severe azotemia after two halothane anesthesia and without exposure to other hepatotoxic drugs or blood transfusions. She was treated with multiple hemodialysis and exchange blood transfusion. The combined treatment corrected the uremic abnormalities and improved her level of consciousness. The liver and kidney function gradually improved, and she made a complete recovery, the first recorded with hepatic and renal failure under these post-anesthetic conditions. Further evaluation of this combined treatment used for this patient is warranted. © 1974 The Japan Surgical Society

Influence of association state and DNA binding on the O2-reactivity of [4Fe-4S] fumarate and nitrate reduction (FNR) regulator

The fumarate and nitrate reduction (FNR) regulator is the master switch for the transition between anaerobic and aerobic respiration in Escherichia coli. Reaction of dimeric [4Fe-4S] FNR with O2 results in conversion of the cluster into a [2Fe-2S] form, via a [3Fe-4S] intermediate, leading to the loss of DNA binding through dissociation of the dimer into monomers. In the present paper, we report studies of two previously identified variants of FNR, D154A and I151A, in which the form of the cluster is decoupled from the association state. In vivo studies of permanently dimeric D154A FNR show that DNA binding does not affect the rate of cluster incorporation into the apoprotein or the rate of O2-mediated cluster loss. In vitro studies show that O2-mediated cluster conversion for D154A and the permanent monomer I151A FNR is the same as in wild-type FNR, but with altered kinetics. Decoupling leads to an increase in the rate of the [3Fe-4S]1+ into [2Fe-2S]2+ conversion step, consistent with the suggestion that this step drives association state changes in the wild-type protein. We have also shown that DNA-bound FNR reacts more rapidly with O2 than FNR free in solution, implying that transcriptionally active FNR is the preferred target for reaction with O2

Eastern Asian emissions of anthropogenic halocarbons deduced from aircraft concentration data

The Montreal Protocol restricts production of ozone-depleting halocarbons worldwide. Enforcement of the protocol has relied mainly on annual government statistics of production and consumption of these compounds (bottom-up approach). We show here that aircraft observations of halocarbon:CO enhancement ratios on regional to continental scales can be used to infer halocarbon emissions, providing independent verification of the bottom-up approach. We apply this top-down approach to aircraft observations of Asian outflow from the TRACE-P mission over the western Pacific (March April 2001) and derive emissions from eastern Asia (China, Japan, and Korea). We derive an eastern Asian carbon tetrachloride (CCl ) source of 21.5 Gg yr , several-fold larger than previous estimates and amounting to 30% of the global budget for this gas. Our emission estimate for CFC-11 from eastern Asia is 50% higher than inventories derived from manufacturing records. Our emission estimates for methyl chloroform (CH ) and CFC-12 are in agreement with existing inventories. For halon 1211 we find only a strong local source originating from the Shanghai area. Our emission estimates for the above gases result in a 40% increase in the ozone depletion potential (ODP) of Asian emissions relative to previous estimates, corresponding to a 10% global increase in ODP

Metabolic Roles of Uncultivated Bacterioplankton Lineages in the Northern Gulf of Mexico "Dead Zone".

This is the final version of the article. Available from American Society for Microbiology via the DOI in this record.Marine regions that have seasonal to long-term low dissolved oxygen (DO) concentrations, sometimes called "dead zones," are increasing in number and severity around the globe with deleterious effects on ecology and economics. One of the largest of these coastal dead zones occurs on the continental shelf of the northern Gulf of Mexico (nGOM), which results from eutrophication-enhanced bacterioplankton respiration and strong seasonal stratification. Previous research in this dead zone revealed the presence of multiple cosmopolitan bacterioplankton lineages that have eluded cultivation, and thus their metabolic roles in this ecosystem remain unknown. We used a coupled shotgun metagenomic and metatranscriptomic approach to determine the metabolic potential of Marine Group II Euryarchaeota, SAR406, and SAR202. We recovered multiple high-quality, nearly complete genomes from all three groups as well as candidate phyla usually associated with anoxic environments-Parcubacteria (OD1) and Peregrinibacteria Two additional groups with putative assignments to ACD39 and PAUC34f supplement the metabolic contributions by uncultivated taxa. Our results indicate active metabolism in all groups, including prevalent aerobic respiration, with concurrent expression of genes for nitrate reduction in SAR406 and SAR202, and dissimilatory nitrite reduction to ammonia and sulfur reduction by SAR406. We also report a variety of active heterotrophic carbon processing mechanisms, including degradation of complex carbohydrate compounds by SAR406, SAR202, ACD39, and PAUC34f. Together, these data help constrain the metabolic contributions from uncultivated groups in the nGOM during periods of low DO and suggest roles for these organisms in the breakdown of complex organic matter.IMPORTANCE Dead zones receive their name primarily from the reduction of eukaryotic macrobiota (demersal fish, shrimp, etc.) that are also key coastal fisheries. Excess nutrients contributed from anthropogenic activity such as fertilizer runoff result in algal blooms and therefore ample new carbon for aerobic microbial metabolism. Combined with strong stratification, microbial respiration reduces oxygen in shelf bottom waters to levels unfit for many animals (termed hypoxia). The nGOM shelf remains one of the largest eutrophication-driven hypoxic zones in the world, yet despite its potential as a model study system, the microbial metabolisms underlying and resulting from this phenomenon-many of which occur in bacterioplankton from poorly understood lineages-have received only preliminary study. Our work details the metabolic potential and gene expression activity for uncultivated lineages across several low DO sites in the nGOM, improving our understanding of the active biogeochemical cycling mediated by these "microbial dark matter" taxa during hypoxia

Charting the Landscape of Modified Gravity

We explore brane induced gravity on a 3-brane in six locally flat dimensions. To regulate the short distance singularities in the brane core, we resolve the thin brane by a cylindrical 4-brane, with the geometry of 4D Minkowski $\times$ a circle, which has an axion flux to cancel the vacuum pressure in the compact direction. We discover a large diversity of possible solutions controlled by the axion flux, as governed by its boundary conditions. Hence brane induced gravity models really give rise to a {\it landscape} of vacua, at least semiclassically. For sub-critical tensions, the crossover scale, below which gravity may look 4D, and the effective 4D gravitational coupling are sensitive to vacuum energy. This shows how the vacuum energy problem manifests in brane induced gravity: instead of tuning the 4D curvature, generically one must tune the crossover scale. On the other hand, in the near-critical limit, branes live inside very deep throats which efficiently compactify the angular dimension. In there, 4D gravity first changes to $5D$, and only later to $6D$. The crossover scale saturates at the gravitational see-saw scale, independent of the tension. Using the fields of static loops on a wrapped brane, we check the perturbative description of long range gravity below the crossover scale. In sub-critical cases the scalars are strongly coupled already at the crossover scale even in the vacuum, because the brane bending is turned on by the axion flux. Near the critical limit, linearized perturbation theory remains under control below the crossover scale, and we find that linearized gravity around the vacuum looks like a scalar-tensor theory.Comment: 47 LaTeX pages, 3 .eps figures, typos fixed to match the published versio

Exact Black Holes and Gravitational Shockwaves on Codimension-2 Branes

We derive exact gravitational fields of a black hole and a relativistic particle stuck on a codimension-2 brane in $D$ dimensions when gravity is ruled by the bulk $D$-dimensional Einstein-Hilbert action. The black hole is locally the higher-dimensional Schwarzschild solution, which is threaded by a tensional brane yielding a deficit angle and includes the first explicit example of a `small' black hole on a tensional 3-brane. The shockwaves allow us to study the large distance limits of gravity on codimension-2 branes. In an infinite locally flat bulk, they extinguish as $1/r^{D-4}$, i.e. as $1/r^2$ on a 3-brane in $6D$, manifestly displaying the full dimensionality of spacetime. We check that when we compactify the bulk, this special case correctly reduces to the 4D Aichelburg-Sexl solution at large distances. Our examples show that gravity does not really obstruct having general matter stress-energy on codimension-2 branes, although its mathematical description may be more involved.Comment: 18 pages, LaTeX; v2: added references, version to appear in JHE

Evidence of precursors of defective measles virus

Cytoplasmic extracts of Vero cells infected with wild strain Edmonston measles virus were found to contain at least two distinct nucleocapsid species. The two most prominent species of nucleocapsids sedimented at 200S and 110S and contained RNA of molecular weight 6.0×10 6 and 0.6×10 6 daltons respectively. Both species of nucleocapsids had a density of 1.31 g/cm 3 in CsCl. A third species sedimenting at 170S was not present in all experiments and was not characterized in detail. Infection of cells with undiluted-passage virus usually resulted in production of mostly 110S nucleocapsids while both 110S and 200S species were found when diluted-passage virus was used. These results suggest that measles virus may produce distinct classes of defective virus which contain segments of RNA representing as little as 10% of the complete viral genome.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47527/1/430_2005_Article_BF02121716.pd

Brane cosmological solutions in six-dimensional warped flux compactifications

We study cosmology on a conical brane in the six-dimensional Einstein-Maxwell-dilaton system, where the extra dimensions are compactified by a magnetic flux. We systematically construct exact cosmological solutions using the fact that the system is equivalently described by (6+n)-dimensional pure Einstein-Maxwell theory via dimensional reduction. In particular, we find a power-law inflationary solution for a general dilatonic coupling. When the dilatonic coupling is given by that of Nishino-Sezgin chiral supergravity, this reduces to the known solution which is not inflating. The power-law solution is shown to be the late-time attractor. We also investigate cosmological tensor perturbations in this model using the (6+n)-dimensional description. We obtain the separable equation of motion and find that there always exist a zero mode, while tachyonic modes are absent in the spectrum. The mass spectrum of Kaluza-Klein modes is obtained numerically.Comment: 12 pages, 2 figures; v2: references added; v3: version published in JCA

Biochemical properties of Paracoccus denitrificans FnrP:Reactions with molecular oxygen and nitric oxide

In Paracoccus denitrificans, three CRP/FNR family regulatory proteins, NarR, NnrR and FnrP, control the switch between aerobic and anaerobic (denitrification) respiration. FnrP is a [4Fe-4S] cluster containing homologue of the archetypal O2 sensor FNR from E. coli and accordingly regulates genes encoding aerobic and anaerobic respiratory enzymes in response to O2, and also NO, availability. Here we show that FnrP undergoes O2-driven [4Fe-4S] to [2Fe-2S] cluster conversion that involves up to 2 O2 per cluster, with significant oxidation of released cluster sulfide to sulfane observed at higher O2 concentrations. The rate of the cluster reaction was found to be ~6-fold lower than that of E. coli FNR, suggesting that FnrP can remain transcriptionally active under microaerobic conditions. This is consistent with a role for FnrP in activating expression of the high O2 affinity cytochrome c oxidase under microaerobic conditions. Cluster conversion resulted in dissociation of the transcriptionally active FnrP dimer into monomers. Therefore, along with E. coli FNR, FnrP belongs to the subset of FNR proteins in which cluster type is correlated with association state. Interestingly, two key charged residues, Arg140 and Asp154, that have been shown to play key roles in the monomer-dimer equilibrium in E. coli FNR are not conserved in FnrP, indicating that different protomer interactions are important for this equilibrium. Finally, the FnrP [4Fe-4S] cluster is shown to undergo reaction with multiple NO molecules, resulting in iron nitrosyl species and dissociation into monomers

Self-assembled photosystem-I biophotovoltaics on nanostructured TiO2 and ZnO

The abundant pigment-protein membrane complex photosystem-I (PS-I) is at the heart of the Earth’s energy cycle. It is the central molecule in the “Z-scheme” of photosynthesis, converting sunlight into the chemical energy of life. Commandeering this intricately organized photosynthetic nanocircuitry and re-wiring it to produce electricity carries the promise of inexpensive and environmentally friendly solar power. We here report that dry PS-I stabilized by surfactant peptides functioned as both the light-harvester and charge separator in solar cells self-assembled on nanostructured semiconductors. Contrary to previous attempts at biophotovoltaics requiring elaborate surface chemistries, thin film deposition, and illumination concentrated into narrow wavelength ranges the devices described here are straightforward and inexpensive to fabricate and perform well under standard sunlight yielding open circuit photovoltage of 0.5 V, fill factor of 71%, electrical power density of 81 µW/cm2 and photocurrent density of 362 µA/cm2, over four orders of magnitude higher than any photosystem-based biophotovoltaic to date