Gyroless Spin-Stabilization Controller and Deorbiting Algorithm for CubeSats

CubeSats are becoming increasingly popular in the scientific community. While they provide a whole new range of opportunities for space exploration, they also come with their own challenges. One of the main concerns is the negative impact which they can have in the space debris problem. Commonly lacking from attitude determination and propulsion capabilities, it has been difficult to provide CubeSats with means for active deorbiting. While electric propulsion technology has been emerging for its application in CubeSats, little or no literature is available on methods to enable it to be used for deorbiting purposes, especially within the tight constraints faced by these nanosatellites. We present a new and simple algorithm for CubeSat deorbiting, which proposes the use of novel electric propulsion technology with minimum sensing and actuation capabilities. The algorithm is divided into two stages: a spin-stabilization control; and a deorbiting-phase detection. The spin-stabilization control is inspired by the B-dot controller. It does not require gyroscopes, but only requires magnetometers and magnetorquers as sensors and actuators, respectively. The deorbiting-phase detection is activated once the satellite is spin-stabilized. The algorithm can be easily implementable as it does not require any attitude information other than the orbital information, e.g., from the Global Positioning System receiver, which could be easily installed in CubeSats. The effectiveness of each part of the algorithms is validated through numerical simulations. The proposed algorithms outperform the existing approaches such as deorbiting sails, inflatable structures, and electrodynamic tethers in terms of deorbiting times. Stability and robustness analysis are also provided. The proposed algorithm is ready to be implemented with minimal effort and provides a robust solution to the space junk mitigation efforts

Actual and ideal roles of school staff to support students with special needs: Current needs and strategies for improvement

To optimise school-based service delivery for students with disabilities, it is important to understand roles and needs of school staff. This study aimed to clarify ideal and actual roles of school staff (teachers, special educators, administrators) working with students with special needs, and to identify potential strategies to support actual roles. Ninety-five school personnel (64% teachers) from 3 different elementary schools and school boards in Quebec completed a 14-question survey. Open-ended responses were coded and analysed thematically. Common actual roles included task adaptation, offering individualized support, being available, and teamwork. Respondents felt roles could improve through in-context professional support, continuing education, teamwork opportunities extending to partnerships with families, and access to resources. Clarifying roles and expectations within a tiered-model to best support students also emerged, emphasizing the importance of sharing responsibilities across all service providers. Findings can guide implementation strategies and processes for providing effective services, enabling inclusion for students

Real-time approaches for characterization of fully and partially scanned canopies in groves

Efficient information management in orchard characterization leads to more efficient agricultural processes. In this brief, a set of computational geometry methods are presented and evaluated for orchard characterization; in particular, for the estimation of canopy volume and shape in groves and orchards using a LiDAR (Light Detection And Ranging) sensor mounted on an agricultural service unit. The proposed approaches were evaluated and validated in the field, showing they are convergent in the estimation process and that they are able to estimate the crown volume for fully scanned canopies in real time; for partially observed tree crowns, accuracy decreases up to 30% (the worst case). The latter is the major contribution of this brief since it implies that the automated service unit does not need to cover all alley-ways for an accurate modeling of the orchard, thus saving valuable resources.The authors would like to thank to CONICYT (Chile): FONDECYT Grant 1140575 and Basal Grant FB0008. Also, this research was partially funded by the Spanish Ministry of Science and Innovation and by the European Union through the FEDER funds (projects Optidosa-AGL2007-66093-C04-03 and Safespray-AGL2010-22304-C04-03)

Extracellular Matrix Aggregates from Differentiating Embryoid Bodies as a Scaffold to Support ESC Proliferation and Differentiation

Embryonic stem cells (ESCs) have emerged as potential cell sources for tissue engineering and regeneration owing to its virtually unlimited replicative capacity and the potential to differentiate into a variety of cell types. Current differentiation strategies primarily involve various growth factor/inducer/repressor concoctions with less emphasis on the substrate. Developing biomaterials to promote stem cell proliferation and differentiation could aid in the realization of this goal. Extracellular matrix (ECM) components are important physiological regulators, and can provide cues to direct ESC expansion and differentiation. ECM undergoes constant remodeling with surrounding cells to accommodate specific developmental event. In this study, using ESC derived aggregates called embryoid bodies (EB) as a model, we characterized the biological nature of ECM in EB after exposure to different treatments: spontaneously differentiated and retinoic acid treated (denoted as SPT and RA, respectively). Next, we extracted this treatment-specific ECM by detergent decellularization methods (Triton X-100, DOC and SDS are compared). The resulting EB ECM scaffolds were seeded with undifferentiated ESCs using a novel cell seeding strategy, and the behavior of ESCs was studied. Our results showed that the optimized protocol efficiently removes cells while retaining crucial ECM and biochemical components. Decellularized ECM from SPT EB gave rise to a more favorable microenvironment for promoting ESC attachment, proliferation, and early differentiation, compared to native EB and decellularized ECM from RA EB. These findings suggest that various treatment conditions allow the formulation of unique ESC-ECM derived scaffolds to enhance ESC bioactivities, including proliferation and differentiation for tissue regeneration applications. © 2013 Goh et al

Immunocompetent 3D Model of Human Upper Airway for Disease Modeling and In Vitro Drug Evaluation

The development of more complex in vitro models for the assessment of novel drugs and chemicals is needed because of the limited biological relevance of animal models to humans as well as ethical considerations. Although some human-cell-based assays exist, they are usually 2D, consist of single cell type, and have limited cellular and functional representation of the native tissue. In this study, we have used biomimetic porous electrospun scaffolds to develop an immunocompetent 3D model of the human respiratory tract comprised of three key cell types present in upper airway epithelium. The three cell types, namely, epithelial cells (providing a physical barrier), fibroblasts (extracellular matrix production), and dendritic cells (immune sensing), were initially grown on individual scaffolds and then assembled into the 3D multicell tissue model. The epithelial layer was cultured at the air–liquid interface for up to four weeks, leading to formation of a functional barrier as evidenced by an increase in transepithelial electrical resistance (TEER) and tight junction formation. The response of epithelial cells to allergen exposure was monitored by quantifying changes in TEER readings and by assessment of cellular tight junctions using immunostaining. It was found that epithelial cells cocultured with fibroblasts formed a functional epithelial barrier at a quicker rate than single cultures of epithelial cells and that the recovery from allergen exposure was also more rapid. Also, our data show that dendritic cells within this model remain viable and responsive to external stimulation as evidenced by their migration within the 3D construct in response to allergen challenge. This model provides an easy to assemble and physiologically relevant 3D model of human airway epithelium that can be used for studies aiming at better understanding lung biology, the cross-talk between immune cells, and airborne allergens and pathogens as well as drug delivery

Transplantation of bioengineered rat lungs recellularized with endothelial and adipose-derived stromal cells

Bioengineered lungs consisting of a decellularized lung scaffold that is repopulated with a patient’s own cells could provide desperately needed donor organs in the future. This approach has been tested in rats, and has been partially explored in porcine and human lungs. However, existing bioengineered lungs are fragile, in part because of their immature vascular structure. Herein, we report the application of adipose-derived stem/stromal cells (ASCs) for engineering the pulmonary vasculature in a decellularized rat lung scaffold. We found that pre-seeded ASCs differentiated into pericytes and stabilized the endothelial cell (EC) monolayer in nascent pulmonary vessels, thereby contributing to EC survival in the regenerated lungs. The ASC-mediated stabilization of the ECs clearly reduced vascular permeability and suppressed alveolar hemorrhage in an orthotopic transplant model for up to 3?h after extubation. Fibroblast growth factor 9, a mesenchyme-targeting growth factor, enhanced ASC differentiation into pericytes but overstimulated their proliferation, causing a partial obstruction of the vasculature in the regenerated lung. ASCs may therefore provide a promising cell source for vascular regeneration in bioengineered lungs, though additional work is needed to optimize the growth factor or hormone milieu for organ culture