Reallabore im Kontext transformativer Forschung : Ansatzpunkte zur Konzeption und Einbettung in den internationalen Forschungsstand

Reallabore sind derzeit ein populärer Forschungsansatz an der Schnittstelle zwischen Wissenschaft und Gesellschaft. Neben vermuteten Potentialen, wie der Erzeugung von Transformationswissen und einem Beitrag zu gesellschaftlichem Wandel, gibt es auch vielfältige offene Fragen, u. a. im Hinblick auf das Verhältnis zu transdisziplinärer Forschung, der Übertragbarkeit des erzeugten Wissens oder der Gestaltung von Experimenten in der "realen Welt". Auch eine breit getragene Definition von Reallaboren existiert derzeit nicht. Dieses Diskussionspapier möchte daher dreierlei leisten: Es beginnt mit dem Herausarbeiten von Reallabor-Merkmalen als a) Beitrag zu Transformationsprozessen, b) Experimenten als zentraler Forschungsmethode, c) Transdisziplinarität als Forschungsmodus, d) dem Ziel der Ausweitung und Übertragung der Ergebnisse und e) Reallaboren als Orten wissenschaftlichen und gesellschaftlichen Lernens. Im Nachgang werden diese Merkmale durch Rückbezug auf die einschlägige Literatur vertieft diskutiert. Abschließend werden mit Reallaboren vergleichbare Forschungsansätze (Sustainable Living Labs, Urban Transition Labs und Niche Experiments) im Hinblick auf ihre Ausgestaltung der fünf herausgearbeiteten Merkmale vorgestellt und Reallabore damit in den internationalen Forschungskontext eingebettet. Das Diskussionspapier will damit Forschenden und Praktiker_innen in Reallaboren eine Orientierung ermöglichen. Die vorgestellten konzeptionellen Überlegungen basieren auf einem thematischen Literatur-Review, ebenso wie Diskussionen mit Reallabor- und Transformationsforschenden in nationalen und internationalen Kontexten. Sie sind im Rahmen der Begleitforschung des Forschungsprojektes "ForReal" zu den Baden-Württembergischen Reallaboren entstanden. Dem Charakter eines Diskussionspapieres entsprechend sind die hier vorstellten Rahmungen zum Reallaboransatz als Angebot zur Diskussion, Erweiterung und Verbesserung zu verstehen

Shaping Real-World Laboratories by Comparing Them

Various experimental approaches of transformative research in real-world settings have emerged. Yet, similarities, differences, and specific contributions remain unclear. A characteristic-based comparison reveals complementarities and provides orientation. Real-world laboratories (RwLs, German Reallabore) belong to a family of increasingly popular experimental and transdisciplinary research approaches at the science-society interface. As these approaches in general, and RwLs in particular, often lack clear definitions of key characteristics and their operationalization, we make two contributions in this article. First, we identify five core characteristics of RwLs: contribution to transformation, experimental methods, transdisciplinary research mode, scalability and transferability of results, as well as scientific and societal learning and reflexivity. Second, we compare RwLs to similar research approaches according to the five characteristics. In this way, we provide an orientation on experimental and transdisciplinary research for societal transformations, and reveal the contributions of this type of research in supporting societal change. Our findings enable learning across the different approaches and highlight their complementarities, with a particular focus on RwLs

Multilocus Detection of Wolf x Dog Hybridization in Italy, and Guidelines for Marker Selection

Hybridization and introgression can impact the evolution of natural populations. Several wild canid species hybridize in nature, sometimes originating new taxa. However, hybridization with free-ranging dogs is threatening the genetic integrity of grey wolf populations (Canis lupus), or even the survival of endangered species (e.g., the Ethiopian wolf C. simensis). Efficient molecular tools to assess hybridization rates are essential in wolf conservation strategies. We evaluated the power of biparental and uniparental markers (39 autosomal and 4 Y-linked microsatellites, a melanistic deletion at the \u3b2-defensin CBD103 gene, the hypervariable domain of the mtDNA control-region) to identify the multilocus admixture patterns in wolf x dog hybrids. We used empirical data from 2 hybrid groups with different histories: 30 presumptive natural hybrids from Italy and 73 Czechoslovakian wolfdogs of known hybrid origin, as well as simulated data. We assessed the efficiency of various marker combinations and reference samples in admixture analyses using 69 dogs of different breeds and 99 wolves from Italy, Balkans and Carpathian Mountains. Results confirmed the occurrence of hybrids in Italy, some of them showing anomalous phenotypic traits and exogenous mtDNA or Y-chromosome introgression. Hybridization was mostly attributable to village dogs and not strictly patrilineal. The melanistic \u3b2-defensin deletion was found only in Italian dogs and in putative hybrids. The 24 most divergent microsatellites (largest wolf-dog FST values) were equally or more informative than the entire panel of 39 loci. A smaller panel of 12 microsatellites increased risks to identify false admixed individuals. The frequency of F1 and F2 was lower than backcrosses or introgressed individuals, suggesting hybridization already occurred some generations in the past, during early phases of wolf expansion from their historical core areas. Empirical and simulated data indicated the identification of the past generation backcrosses is always uncertain, and a larger number of ancestry-informative markers is needed

GSK-3\u3b2-induced Tau pathology drives hippocampal neuronal cell death in Huntington's disease: involvement of astrocyte-neuron interactions

Glycogen synthase kinase-3\u3b2 (GSK-3\u3b2) has emerged as a critical factor in several pathways involved in hippocampal neuronal maintenance and function. In Huntington's disease (HD), there are early hippocampal deficits both in patients and transgenic mouse models, which prompted us to investigate whether disease-specific changes in GSK-3\u3b2 expression may underlie these abnormalities. Thirty-three postmortem hippocampal samples from HD patients (neuropathological grades 2-4) and age- and sex-matched normal control cases were analyzed using real-time quantitative reverse transcription PCRs (qPCRs) and immunohistochemistry. In vitro and in vivo studies looking at hippocampal pathology and GSK-3\u3b2 were also undertaken in transgenic R6/2 and wild-type mice. We identified a disease and stage-dependent upregulation of GSK-3\u3b2 mRNA and protein levels in the HD hippocampus, with the active isoform pGSK-3\u3b2-Tyr(216) being strongly expressed in dentate gyrus (DG) neurons and astrocytes at a time when phosphorylation of Tau at the AT8 epitope was also present in these same neurons. This upregulation of pGSK-3\u3b2-Tyr(216) was also found in the R6/2 hippocampus in vivo and linked to the increased vulnerability of primary hippocampal neurons in vitro. In addition, the increased expression of GSK-3\u3b2 in the astrocytes of R6/2 mice appeared to be the main driver of Tau phosphorylation and caspase3 activation-induced neuronal death, at least in part via an exacerbated production of major proinflammatory mediators. This stage-dependent overactivation of GSK-3\u3b2 in HD-affected hippocampal neurons and astrocytes therefore points to GSK-3\u3b2 as being a critical factor in the pathological development of this condition. As such, therapeutic targeting of this pathway may help ameliorate neuronal dysfunction in HD

Sistema de información de tráfico vehicular en rutas

La presente publicación trata de un proyecto ya en curso que consiste en desarrollar el hardware y software de un sistema de información para la adquisición y análisis de datos de tráfico vehicular en rutas pavimentadas. El mismo se compone de varios subsistemas, entre ellos, el de adquisición de datos "in situ", el de transmisión inalámbrica y el de almacenamiento y procesamiento. Mediante sensores instalados en ruta, se toman datos sobre los vehículos que atraviesan una determinada arteria (sin interrupción del flujo vehicular), se analizan y procesan los datos y se los envía en forma remota a una central para la elaboración de informes estadísticos. Este tipo de tecnología será de gran importancia ya que permitirá caracterizar el flujo vehicular, permitiendo administrar y planificar el uso, mantenimiento y construcción de nuevas y mejores rutas, además de aportar herramientas para aumentar la seguridad en las mismas.Sociedad Argentina de Informática e Investigación Operativ

Efavirenz versus boosted atazanavir-containing regimens and immunologic, virologic, and clinical outcomes: A prospective study of HIV-positive individuals

OBJECTIVE: To compare regimens consisting of either ritonavir-boosted atazanavir or efavirenz and a nucleoside reverse transcriptase inhibitor (NRTI) backbone with respect to clinical, immunologic, and virologic outcomes. DESIGN: Prospective studies of human immunodeficiency virus (HIV)-infected individuals in Europe and the United States included in the HIV-CAUSAL Collaboration. METHODS: HIV-positive, antiretroviral therapy-naive, and acquired immune deficiency syndrome (AIDS)-free individuals were followed from the time they started an atazanavir or efavirenz regimen. We estimated an analog of the "intention-to-treat" effect for efavirenz versus atazanavir regimens on clinical, immunologic, and virologic outcomes with adjustment via inverse probability weighting for time-varying covariates. RESULTS: A total of 4301 individuals started an atazanavir regimen (83 deaths, 157 AIDS-defining illnesses or deaths) and 18,786 individuals started an efavirenz regimen (389 deaths, 825 AIDS-defining illnesses or deaths). During a median follow-up of 31 months, the hazard ratios (95% confidence intervals) were 0.98 (0.77, 1.24) for death and 1.09 (0.91, 1.30) for AIDS-defining illness or death comparing efavirenz with atazanavir regimens. The 5-year survival difference was 0.1% (95% confidence interval: -0.7%, 0.8%) and the AIDS-free survival difference was -0.3% (-1.2%, 0.6%). After 12 months, the mean change in CD4 cell count was 20.8 (95% confidence interval: 13.9, 27.8) cells/mm lower and the risk of virologic failure was 20% (14%, 26%) lower in the efavirenz regimens. CONCLUSION: Our estimates are consistent with a smaller 12-month increase in CD4 cell count, and a smaller risk of virologic failure at 12 months for efavirenz compared with atazanavir regimens. No overall differences could be detected with respect to 5-year survival or AIDS-free survival

Jointly experimenting for transformation?: Shaping real-world laboratories by comparing them

Real-world laboratories (RwLs, German Reallabore) belong to a family of increasingly popular experimental and transdisciplinary research approaches at the science-society interface. As these approaches in general, and RwLs in particular, often lack clear definitions of key characteristics and their operationalization, we make two contributions in this article. First, we identify five core characteristics of RwLs: contribution to transformation, experimental methods, transdisciplinary research mode, scalability and transferability of results, as well as scientific and societal learning and reflexivity. Second, we compare RwLs to similar research approaches according to the five characteristics. In this way, we provide an orientation on experimental and transdisciplin ary research for societal transformations, and reveal the contributions of this type of research in supporting societal change. Our findings enable learning across the different approaches and highlight their complementarities, with a particular focus on RwLs