144 research outputs found
Numerical analysis of seismic wave amplification in Nice (France) and comparisons with experiments
The analysis of site effects is very important since the amplification of
seismic motion in some specific areas can be very strong. In this paper, the
site considered is located in the centre of Nice on the French Riviera. Site
effects are investigated considering a numerical approach (Boundary Element
Method) and are compared to experimental results (weak motion and
microtremors). The investigation of seismic site effects through numerical
approaches is interesting because it shows the dependency of the amplification
level on such parameters as wave velocity in surface soil layers, velocity
contrast with deep layers, seismic wave type, incidence and damping. In this
specific area of Nice, a one-dimensional (1D) analytical analysis of
amplification does not give a satisfactory estimation of the maximum reached
levels. A boundary element model is then proposed considering different wave
types (SH, P, SV) as the seismic loading. The alluvial basin is successively
assumed as an isotropic linear elastic medium and an isotropic linear
viscoelastic solid (standard solid). The thickness of the surface layer, its
mechanical properties, its general shape as well as the seismic wave type
involved have a great influence on the maximum amplification and the frequency
for which it occurs. For real earthquakes, the numerical results are in very
good agreement with experimental measurements for each motion component.
Two-dimensional basin effects are found to be very strong and are well
reproduced numerically
Wave propagation through soils in centrifuge testing.
International audienceWave propagation phenomena in soils can be experimentally simulated using centrifuge scale models. An original excitation device (drop-ball arrangement) is proposed to generate short wave trains. Wave reflections on model boundaries are taken into account and removed by homomorphic filtering. Propagation is investigated through dispersion laws. For drop-ball experiments, spherical wave field analysis assuming linear viscoelasticity leads to a complete analytical description of wave propagation. Damping phenomena are examined and evaluated using this description
A Fast Multipole Method formulation for 3D elastodynamics in the frequency domain
The solution of the elastodynamic equations using boundary element methods (BEMs) gives rise to fully-populated matrix equations. Earlier investigations on the Helmholtz and Maxwell equations have established that the Fast Multipole (FM) method reduces the complexity of a BEM solution to N \mbox{log}_{2}N per GMRES teration. The present Note address the extension of the FM-BEM strategy to 3D elastodynamics in the frequency domain. Its efficiency and accuracy are demonstrated on numerical examples involving up to nodal unknowns
Sollicitations sismiques dues aux exploitations minières : amplification des ondes en surface (et vibrations des structures)
National audienceL'objectif de ce travail est d'analyser l'impact des secousses induites par les exploitations minières sur les structures situées en surface. Du fait des conditions géologiques locales, le mouvement créé par les secousses peut parfois être amplifié de façon importante en surface (effet de site). A partir d'une modélisation numérique du problème, l'amplification du mouvement est analysée pour différentes formes de remplissages sédimentaires et des contrastes de propriétés entre couches variables. Les valeurs de fréquences fondamentales (i.e amplification maximale) sont comparées à des résultats antérieurs
3D Hopkinson bar: new experiments for dynamic testing on soils.
International audienceThe direct analysis of the dynamic response of materials is possible using Split Hopkinson pressure bar method. For soils, it has to be adapted since the specimen has generally poor mechanical properties. An original experimental arrangement called "Three-Dimensional Split Hopkinson Pressure Bar" (3D SHPB) is proposed. It allows the measurement of the complete three-dimensional dynamic response of soils. Different types of confinement systems are used. The results on different loading paths are compared with other works on sand and clay. The analysis at grain-size level gives further elements on the comminution process
Modeling seismic wave propagation and amplification in 1D/2D/3D linear and nonlinear unbounded media
To analyze seismic wave propagation in geological structures, it is possible
to consider various numerical approaches: the finite difference method, the
spectral element method, the boundary element method, the finite element
method, the finite volume method, etc. All these methods have various
advantages and drawbacks. The amplification of seismic waves in surface soil
layers is mainly due to the velocity contrast between these layers and,
possibly, to topographic effects around crests and hills. The influence of the
geometry of alluvial basins on the amplification process is also know to be
large. Nevertheless, strong heterogeneities and complex geometries are not easy
to take into account with all numerical methods. 2D/3D models are needed in
many situations and the efficiency/accuracy of the numerical methods in such
cases is in question. Furthermore, the radiation conditions at infinity are not
easy to handle with finite differences or finite/spectral elements whereas it
is explicitely accounted in the Boundary Element Method. Various absorbing
layer methods (e.g. F-PML, M-PML) were recently proposed to attenuate the
spurious wave reflections especially in some difficult cases such as shallow
numerical models or grazing incidences. Finally, strong earthquakes involve
nonlinear effects in surficial soil layers. To model strong ground motion, it
is thus necessary to consider the nonlinear dynamic behaviour of soils and
simultaneously investigate seismic wave propagation in complex 2D/3D geological
structures! Recent advances in numerical formulations and constitutive models
in such complex situations are presented and discussed in this paper. A crucial
issue is the availability of the field/laboratory data to feed and validate
such models.Comment: of International Journal Geomechanics (2010) 1-1
Involvement of Plasmodium falciparum protein kinase CK2 in the chromatin assembly pathway
<p>Abstract</p> <p>Background</p> <p>Protein kinase CK2 is a pleiotropic serine/threonine protein kinase with hundreds of reported substrates, and plays an important role in a number of cellular processes. The cellular functions of <it>Plasmodium falciparum </it>CK2 (PfCK2) are unknown. The parasite's genome encodes one catalytic subunit, PfCK2α, which we have previously shown to be essential for completion of the asexual erythrocytic cycle, and two putative regulatory subunits, PfCK2β1 and PfCK2β2.</p> <p>Results</p> <p>We now show that the genes encoding both regulatory PfCK2 subunits (PfCK2β1 and PfCK2β2) cannot be disrupted. Using immunofluorescence and electron microscopy, we examined the intra-erythrocytic stages of transgenic parasite lines expressing hemagglutinin (HA)-tagged catalytic and regulatory subunits (HA-CK2α, HA-PfCK2β1 or HA-PfCK2β2), and localized all three subunits to both cytoplasmic and nuclear compartments of the parasite. The same transgenic parasite lines were used to purify PfCK2β1- and PfCK2β2-containing complexes, which were analyzed by mass spectrometry. The recovered proteins were unevenly distributed between various pathways, with a large proportion of components of the chromatin assembly pathway being present in both PfCK2β1 and PfCK2β2 precipitates, implicating PfCK2 in chromatin dynamics. We also found that chromatin-related substrates such as nucleosome assembly proteins (Naps), histones, and two members of the Alba family are phosphorylated by PfCK2α <it>in vitro</it>.</p> <p>Conclusions</p> <p>Our reverse-genetics data show that each of the two regulatory PfCK2 subunits is required for completion of the asexual erythrocytic cycle. Our interactome study points to an implication of PfCK2 in many cellular pathways, with chromatin dynamics being identified as a major process regulated by PfCK2. This study paves the way for a kinome-wide interactomics-based approach to elucidate protein kinase function in malaria parasites.</p
Effet de la pression interstitielle sur la réponse sismique des sols : modélisation numérique 1D-3 Composantes
International audienceDuring strong quakes, the propagation of seismic waves in soil layers involves nonlinearities changing with the excitation level. A nonlinear hysteretic law is necessary to describe the variations of the stiffness and the energy dissipation during the seismic shaking. Furthermore, the influence of the pore pressure (cyclic mobility and liquefaction) cannot be neglected for saturated soils under strong quakes. Starting from a FEM formulation describing 1D propagation and three-dimensional loading ("1D-3 components approach"), the influence of the water is accounted for through a relation between the pore pressure and the work of the shear stress initially proposed by Iai. This model describes the variations of the pore pressure from the three-dimensional stress state of the soil. It has been validated through comparisons to laboratory tests (cyclic triaxial tests on saturated sands) and an analysis under three-dimensional excitations (seismic loading polarized along the 3 directions of space). The results involving 3 simultaneous excitation components and a single component in 3 separated analyses show the influence of the loading path on the seismic response and the pore pressure build-up
An atypical cyclin-dependent kinase controls Plasmodium falciparum proliferation rate
Malaria parasites multiply in human erythrocytes through schizogony, a process characterised by nuclear divisions in the absence of cytokinesis, leading to the formation of a multinucleated schizont from which individual daughter cells are subsequently generated. Here, we provide evidence that parasites lines lacking Pfcrk-5, an atypical cyclin-dependent kinase, display a reduced parasitemia growth rate linked to a decrease in the number of daughter nuclei produced by each schizont. We show that in vitro activity of recombinant Pfcrk-5 is indeed cyclin-dependent and that the enzyme localises to the nuclear periphery. Thus, Pfcrk-5 is part of a regulatory pathway that mediates the proliferation rate of Plasmodium falciparum through the control of nuclear divisions during schizogony
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