Dual action of a dinoflagellate-derived precursor of Pacific ciguatoxins (P-CTX-4B) on voltage-dependent K(+) and Na(+) channels of single myelinated axons

The effects of Pacific ciguatoxin-4B (P-CTX-4B, also named gambiertoxin), extracted from toxic Gambierdiscus dinoflagellates, were assessed on nodal K(+) and Na(+) currents of frog myelinated axons, using a conventional voltage-clamp technique. P-CTX-4B decreased, within a few minutes, both K(+) and Na(+) currents in a dose-dependent manner, without inducing any marked change in current kinetics. The toxin was more effective in blocking K(+) than Na(+) channels. P-CTX-4B shifted the voltage-dependence of Na(+) conductance by about 14 mV towards more negative membrane potentials. This effect was reversed by increasing Ca(2+) in the external solution. A negative shift of about 16 mV in the steady-state Na(+) inactivation-voltage curve was also observed in the presence of the toxin. Unmodified and P-CTX-4B-modified Na(+) currents were similarly affected by the local anaesthetic lidocaine. The decrease of the two currents by lidocaine was dependent on both the concentration and the membrane potential during pre-pulses. In conclusion, P-CTX-4B appears about four times more effective than P-CTX-1B to affect K(+) channels, whereas it is about 50 times less efficient to affect Na(+) channels of axonal membranes. These actions may be related to subtle differences between the two chemical structures of molecules

A qualitative view of cryogenic fluid injection into high speed flows

The injection of supercritical pressure, subcritical temperature fluids, into a 2-D, ambient, static temperature and static pressure supersonic tunnel and free jet supersonic nitrogen flow field was observed. Observed patterns with fluid air were the same as those observed for fluid nitrogen injected into the tunnel at 90 deg to the supersonic flow. The nominal injection pressure was of 6.9 MPa and tunnel Mach number was 2.7. When injected directly into and opposing the tunnel exhaust flow, the observed patterns with fluid air were similar to those observed for fluid nitrogen but appeared more diffusive. Cryogenic injection creates a high density region within the bow shock wake but the standoff distance remains unchanged from the gaseous value. However, as the temperature reaches a critical value, the shock faded and advanced into the supersonic stream. For both fluids, nitrogen and air, the phenomena was completely reversible

Estimulación vagal en el tratamiento de la epilepsia

The vagal nerve stimulation is a new technique for the treatment of drug resistant epilepsies. DEVELOPMENT: In 1997, it was approved in United States by the FDA to be used in adults with refractory focal epilepsies not candidates for epilepsy surgery. Its mechanism of action is unknown. The results in the controlled studies indicated a decrease of 30 50% in the seizure frequency in around 50% of the patients. Although more experience is needed to corroborate these results, it seems reasonable as a treatment for patients with difficult epilepsies, especially when the response to the antiepileptic drugs is poor or they are producing secondary effects, and the resection of the focus is not possible

Utilisation du phytoplancton pour estimer la production piscicole potentielle des étangs

Pour faciliter la gestion des étangs de pisciculture, il est nécessaire d'avoir un moyen pour estimer en cours de saison si l'objectif de production sera atteint, et éventuellement, intervenir sur la qualité du milieu. Les méthodes d'estimation et de prévision mentionnées dans la littérature ne sont pas applicables aux étangs de pisciculture, milieux fortement anthropisés. A partir de données (physico-chimie, plancton, production piscicole) collectées sur 36 étangs en France, les auteurs proposent une méthode de prévision de la récolte piscicole à partir du phytoplancton (groupe prédominant et teneur en chlorophylle a). A l'usage, cette méthode s'est avérée satisfaisante pour prévoir des productions nettes jusqu'à plus de 500 kg/hectare

Trastornos específicos del desarrollo del lenguaje: bases neurobiológicas

Studies of twins, familial aggregates and particular phenotypic conditions have shown an inherited basis for some dysphasias or specific developmental language impairments (SLI). This predisposition is usually multifactorial but the analysis of some families allows to postulate an autosomal dominant transmission of deficits in specific modular aspects of linguistic competences. Moreover, neuroimaging studies have shown modifications of normal volumetric interhemispheric asymmetries, and in group of SLI with receptive prominent disorder coexist epileptiform activity in wakefulness and non-REM sleep EEG; in some of these cases, antiepileptic drugs, specially steroids, can significantly ameliorate the language processing. As many patients with SLI have a difficulty for discrimination of subtle temporal indices, a hypothesis can also be made of a dysfunction in various subcortical structures (thalamus, basal ganglia, cerebellum) modulating the cerebral cortex in phonological processing

Some preliminary results of brush seal/rotor interference effects on leakage at zero and low RPM using a tapered-plug rotor

Some preliminary brush seal leakage results for ambient temperature air are presented. Data for four nominal brush rotor radial clearances of -0.09, -0.048, -0.008, and 0.035 mm were taken by using a tapered plug rotor at 0 and 400 rpm with rotor runout of 0.127 mm peak to peak. The brush seal nominal bore diameter was 38 mm with 0.05 mm bristles at 200 bristles/mm of circumference and a 0.61 mm fence height. Leakages were greater than predicted, but agreement was reasonable. Leakage rates were not significantly altered by hysteresis or inlet flow variations. Visualization studies showed that the bristles followed the 400 rpm excitation, and loading studies indicated that bristles slid relative to one another