In vitro propagation of Solidago virgaurea L. through nodal culture

A reliable in vitro propagation protocol has been established from nodal segmentsof a highly valuable medicinal plant Solidago virgaurea L. The explants were cultured onMurashige and Skoog’s medium augmented with different concentrations andcombinations of plant growth regulators for shoot bud initiation and multiplications.Highest frequency of shoot proliferation (91.8 ± 0.64) and maximum number (12.1 ± 0.20) pernode was observed in Murashige and Skoog’s medium augmented with4.5 mg/l of Kinetinin combination with 0.5 mg/l of Indole -3- Acetic acid. Highest frequency (91.7 ± 1.43) ofrooting and maximum number (3.8 ± 0.28) of rootlet per shoot let was achieved onMurashige and Skoog’s medium fortified with 4.5 mg/l of Indole -3- Acetic acid incombination with 0.5 mg/l of Benzyl -6- amino purine. Regenerated plants weresuccessfully transferred to field (78%)

Effect of educational intervention on the knowledge and attitude on prescribing amongst interns of a tertiary care hospital: a questionnaire based study

Background: Prescribing correct therapy is the essential part of physicians and therefore, adequate knowledge and confidence on drugs efficacy, safety, cost, and convenience is important. Understanding the knowledge and perceptions of medical students will help to identify the problems in clinical pharmacology teaching. Prescribing performance and competence can be significantly improved by training and giving feedback to the prescriber. The objective of the study was to evaluate and compare the effect of educational intervention in enhancing the knowledge of medical interns on prescribing practice.Methods: A Cross sectional, Educational Interventional observational study was conducted using two copies of a pretested validated questionnaire (Pre & Post CME) with 15 questions among 110 MBBS interns. They were subjected to a continuous medical education on rational prescription writing followed by a hand’s on training on prescription writing in which they were divided into 9 batches and asked to prescribe for the case scenario given to them and the same was audited and feedback was given.Results: Out of 110 internees’, 105 (95.50%) and 95 (96.90%) had completely filled the pre & post educational intervention questionnaire. The overall knowledge and attitude of interns has improved after the intervention. Significant increase (P value < 0.0001) in the Mean + Standard deviation value from 5.81 + 2.1 to 7.35 + 3.18 is noted on applying Paired t Test. All the prescriptions were rational. The formats of their prescriptions were scrutinized and the precise way of writing the same was explained to the interns.Conclusions: This study clearly shows that early sensitization of Physicians about rational prescription writing during Internship phase by means of educational intervention/training program can reduce the number of prescription errors

Investigation on Battery for Electric Vehicle Application

Battery powered electric vehicles are becoming increasingly important in the automotive industry. It is hard to decide which battery meets all the most important characteristics from different perspectives such as energy storage efficiency, construction characteristics, cost price, safety and service life of today’s Electric Vehicles. Investigation on different types of batteries are discussed in this paper. Among the most important components of a car is the battery. Only the stored electrical energy in the battery provides the power for BEVs which is the unique source of energy. Various types of batteries are used in electric cars depending on their systems. The lithium-ion battery is the most common electric car battery. A battery which is considered zero emission is abbreviated as ZEBRA. In this paper, we can gain knowledge over the different types of battery options available for electric vehicles, along with their characteristics

Bioactive potential of selected actinobacterial strains against Mycobacterium tuberculosis and other clinical pathogens

1307-1311Marine actinobacteria produces diverse array of metabolites with novel chemical structures with potential bioactivities. Exploring the understudied ecosystems may increase the chance of getting novel actinobacteria and new metabolites.The present study explores the bioactive potential of actinobacteria isolated from the marine ecosystem of Andaman and Nicobar Islands, Bay of Bengal, against Mycobacterium tuberculosis and other clinical pathogens. The crude extracts from 15 marine actinobacterial strains were produced through agar surface fermentation using YEME agar and extracted using ethyl acetate. The crude extracts were tested against the standard strain M. tuberculosis H37Rv, clinical drug sensitive M. tuberculosis, and MDR M. tuberculosis strains by luciferase reporter phage (LRP) assay at 500 µg/ml concentration. The anti-microbial activity against other clinical pathogens, namely, Staphylococcus aureus, Escherichia coli, Salmonella paratyphi, Klebsiellapneumoniae, Pseudomonas aeruginosa, Candida albicans, and Cryptococcusneoformans and non-tubercular mycobacteria, M. smegmatis was studied by agar plug method. Among the 15 extracts that were tested for anti-tubercular activity, the crude ethyl acetate extract of the 14 actinobacterial strains showed anti-tubercular activity against at least one of the three M. tuberculosis strains. Exceptionally, the ethyl acetate extract of strain SACC 168 inhibited all three M. tuberculosis strains tested. In anti-microbial screening, the crude extracts of eight strains showed anti-microbial activity including six strains, which were active against the non-tuberculous mycobacteria. Further purification and characterization of the active molecule from the potential extracts will pave way for the potential natural product candidate for tuberculosis and other microbial infections

Plasma Concentrations of Myeloperoxidase Predict Mortality After Myocardial Infarction

ObjectivesThis study investigated relationships between plasma myeloperoxidase (MPO), protein oxidation markers, and clinical outcome retrospectively in patients after acute myocardial infarction (MI).BackgroundReactive oxidants are implicated in cardiovascular disease, and elevated plasma MPO is reported to predict adverse outcome in acute coronary syndromes.MethodsDetailed demographic information, radionuclide ventriculography, neurohormone measurements, and clinical history were obtained for 512 acute MI patients at hospital admission. Plasma levels of MPO and protein carbonyls were measured in patients and 156 heart-healthy control subjects. 3-Chlorotyrosine was measured in selected patients. Patient mortality was followed for 5 years.ResultsPlasma MPO and protein carbonyl concentrations were higher in MI patients 24 h to 96 h after admission than in control subjects (medians: MPO 55 ng/ml vs. 39 ng/ml, and protein carbonyls 48 pmol/mg vs. 17 pmol/mg protein, p < 0.001 for each). Both markers were significantly correlated with each other and with cardiovascular hormone levels. Chlorotyrosine was not elevated in patients with high MPO or carbonyl levels. Above-median levels of MPO but not protein carbonyls were independently predictive of mortality (odds ratio 1.8, 95% confidence interval 1.0 to 3.0, p = 0.034). Patients with above-median MPO levels in combination with above-median plasma amino-terminal pro-brain natriuretic peptide (NT-proBNP) or below-median left ventricular ejection fraction (LVEF) had significantly greater mortality compared with other patients.ConclusionsMyeloperoxidase and protein carbonyl levels are elevated in plasma after acute MI, apparently via independent mechanisms. High MPO is a risk factor for long-term mortality and adds prognostic value to LVEF and plasma NT-proBNP measurements

Myeloperoxidase and oxidative stress in rheumatoid arthritis

Objective. To determine whether MPO contributes to oxidative stress and disease activity in RA and whether it produces hypochlorous acid in SF. Methods. Plasma and where possible SF were collected from 77 RA patients while 120 healthy controls supplied plasma only. MPO and protein carbonyls were measured by ELISAs. 3-Chlorotyrosine in proteins and allantoin in plasma were measured by mass spectrometry. Results. Plasma MPO concentrations were significantly higher in patients with RA compared with healthy controls [10.8 ng/ml, inter-quartile range (IQR): 7.214.2; P < 0.05], but there was no significant difference in plasma MPO protein concentrations between RA patients with high disease activity (HDA; DAS-28 >3.2) and those with low disease activity (LDA; DAS-28 43.2) (HDA 27.9 ng/ml, 20.234.1 vs LDA 22.1 ng/ml, 16.934.9; P > 0.05). There was a significant relationship between plasma MPO and DAS-28 (r = 0.35; P = 0.005). Plasma protein carbonyls and allantoin were significantly higher in patients with RA compared with the healthy controls. MPO protein was significantly higher in SF compared with plasma (median 624.0 ng/ml, IQR 258.42433.0 vs 30.2 ng/ml, IQR 25.150.9; P < 0.0001). The MPO present in SF was mostly active. 3-Chlorotyrosine, a specific biomarker of hypochlorous acid, was present in proteins from SF and related to the concentration of MPO (r = 0.69; P = 0.001). Protein carbonyls in SF were associated with MPO protein concentration (r = 0.40; P = 0.019) and 3-chlorotyrosine (r = 0.66; P = 0.003). Conclusion. MPO is elevated in patients with RA and promotes oxidative stress through the production of hypochlorous acid

The universally growing mode in the solar atmosphere: coronal heating by drift waves

The heating of the plasma in the solar atmosphere is discussed within both frameworks of fluid and kinetic drift wave theory. We show that the basic ingredient necessary for the heating is the presence of density gradients in the direction perpendicular to the magnetic field vector. Such density gradients are a source of free energy for the excitation of drift waves. We use only well established basic theory, verified experimentally in laboratory plasmas. Two mechanisms of the energy exchange and heating are shown to take place simultaneously: one due to the Landau effect in the direction parallel to the magnetic field, and another one, stochastic heating, in the perpendicular direction. The stochastic heating i) is due to the electrostatic nature of the waves, ii) is more effective on ions than on electrons, iii) acts predominantly in the perpendicular direction, iv) heats heavy ions more efficiently than lighter ions, and v) may easily provide a drift wave heating rate that is orders of magnitude above the value that is presently believed to be sufficient for the coronal heating, i.e., $\simeq 6 \cdot 10^{-5}$J/(m$^3$s) for active regions and $\simeq 8 \cdot 10^{-6}$J/(m$^3$s) for coronal holes. This heating acts naturally through well known effects that are, however, beyond the current standard models and theories.Comment: To appear in MNRA

Accuracy of CT Colonography for Detection of Large Adenomas and Cancers

Background Computed tomographic (CT) colonography is a noninvasive option in screening for colorectal cancer. However, its accuracy as a screening tool in asymptomatic adults has not been well defined. Methods We recruited 2600 asymptomatic study participants, 50 years of age or older, at 15 study centers. CT colonographic images were acquired with the use of standard bowel preparation, stool and fluid tagging, mechanical insufflation, and multidetector-row CT scanners (with 16 or more rows). Radiologists trained in CT colonography reported all lesions measuring 5 mm or more in diameter. Optical colonoscopy and histologic review were performed according to established clinical protocols at each center and served as the reference standard. The primary end point was detection by CT colonography of histologically confirmed large adenomas and adenocarcinomas (10 mm in diameter or larger) that had been detected by colonoscopy; detection of smaller colorectal lesions (6 to 9 mm in diameter) was also evaluated. Results Complete data were available for 2531 participants (97%). For large adenomas and cancers, the mean (±SE) per-patient estimates of the sensitivity, specificity, positive and negative predictive values, and area under the receiver-operating-characteristic curve for CT colonography were 0.90±0.03, 0.86±0.02, 0.23±0.02, 0.99± Conclusions In this study of asymptomatic adults, CT colonographic screening identified 90% of subjects with adenomas or cancers measuring 10 mm or more in diameter. These findings augment published data on the role of CT colonography in screening patients with an average risk of colorectal cancer. (ClinicalTrials.gov number, NCT00084929; American College of Radiology Imaging Network [ACRIN] number, 6664.