144 research outputs found
Elevated genetic diversity in an F2:6 population of quinoa (<i>Chenopodium quinoa</i>) developed through an inter-ecotype cross
Quinoa (Chenopodium quinoa) is a seed crop of the Andean highlands and Araucanian coastal regions of South America that has recently expanded in use and production beyond its native range. This is largely due its superb nutritional value, consisting of protein that is rich in essential amino acids along with vitamins and minerals. Quinoa also presents a remarkable degree of tolerance to saline conditions, drought, and frost. The present study involved 72 F2:6 recombinant inbred lines (RIL) and parents developed through hybridization between highland (0654) and coastal (NL-6) germplasm groups. The purpose was to characterize the quinoa germplasm developed, to assess the discriminating potential of 21 agro-morpho phenological traits, and to evaluate the extent of genetic variability recovered through selfing. A vast amount of genetic variation was detected among the 72 lines evaluated for quantitative and qualitative traits. Impressive transgressive segregation was measured for seed yield (22.42 g/plant), while plant height and maturity had higher heritabilities (73 and 89%, respectively). Other notable characters segregating in the population included panicle and stem color, panicle form, and resistance to downy mildew. In the Principal Component analysis, the first axis explained 74% of the total variation and was correlated to plant height, panicle size, stem diameter, biomass, mildew reaction, maturation, and seed yield; those traits are relevant discriminatory characters. Yield correlated positively with panicle length and biomass. UPGMA based cluster analysis identified three groups: one consisting of late, mildew-resistant, high yielding lines; one having semi-late lines with intermediate yield and mildew susceptibility; and a third cluster consisting of early to semi-late accessions with low yield and mildew susceptibility. This study highlighted the extended diversity regenerated among the 72 accessions and helped to identify potentially adapted quinoa genotypes for production in the Moroccan coastal environment
A Chromosome-Scale Assembly of the Garden Orach (Atriplex hortensis L.) Genome Using Oxford Nanopore Sequencing
Atriplex hortensis (2n = 2x = 18, 1C genome size 1.1 gigabases), also known
as garden orach and mountain-spinach, is a highly nutritious, broadleaf annual of
the Amaranthaceae-Chenopodiaceae alliance (Chenopodiaceae sensu stricto, subfam.
Chenopodioideae) that has spread in cultivation from its native primary domestication
area in Eurasia to other temperate and subtropical regions worldwide. Atriplex L. is a
highly complex but, as understood now, a monophyletic group of mainly halophytic
and/or xerophytic plants, of which A. hortensis has been a vegetable of minor
importance in some areas of Eurasia (from Central Asia to the Mediterranean) at least
since antiquity. Nonetheless, it is a crop with tremendous nutritional potential due
primarily to its exceptional leaf and seed protein quantities (approaching 30%) and
quality (high levels of lysine). Although there is some literature describing the taxonomy
and production of A. hortensis, there is a general lack of genetic and genomic data
that would otherwise help elucidate the genetic variation, phylogenetic positioning, and
future potential of the species. Here, we report the assembly of the first high-quality,
chromosome-scale reference genome for A. hortensis cv. “Golden.” Long-read data
from Oxford Nanopore’s MinION DNA sequencer was assembled with the program
Canu and polished with Illumina short reads. Contigs were scaffolded to chromosome
scale using chromatin-proximity maps (Hi-C) yielding a final assembly containing 1,325
scaffolds with a N50 of 98.9 Mb – with 94.7% of the assembly represented in the nine
largest, chromosome-scale scaffolds. Sixty-six percent of the genome was classified
as highly repetitive DNA, with the most common repetitive elements being Gypsy-
(32%) and Copia-like (11%) long-terminal repeats. The annotation was completed using
MAKER which identified 37,083 gene models and 2,555 tRNA genes. Completeness of the genome, assessed using the Benchmarking Universal Single Copy Orthologs
(BUSCO) metric, identified 97.5% of the conserved orthologs as complete, with only
2.2% being duplicated, reflecting the diploid nature of A. hortensis. A resequencing
panel of 21 wild, unimproved and cultivated A. hortensis accessions revealed three
distinct populations with little variation within subpopulations. These resources provide
vital information to better understand A. hortensis and facilitate future study
Creativity encounters between children and robots
Creativity is an intrinsic human ability with multiple benefits across the lifespan. Despite its importance, societies not always are well equipped with contexts for creativity stimulation; as a consequence, a major decline in creative abilities occurs at the age of 7 years old. We investigated the effectiveness of using a robotic system named YOLO as an intervention tool to stimulate creativity in children. During the intervention, children used YOLO as a character for their stories and through the interaction with the robot, creative abilities were stimulated. Our study (n = 62) included 3 experimental conditions: i) YOLO displayed behaviors based on creativity techniques; ii) YOLO displayed behaviors based on creativity techniques plus social behaviors; iii) YOLO was turned off, not displaying any
behaviors. We measured children’s creative abilities at pre- and post-testing and their creative process through behavior analysis. Results showed that the interaction with YOLO contributed to higher creativity levels in children, specifically contributing to the generation of more original ideas during story creation. This study shows the potential of using social robots as tools to empower intrinsic human abilities, such as the ability to be creative.info:eu-repo/semantics/publishedVersio
Genomic insights from the first chromosome-scale assemblies of oat (Avena spp.) diploid species
Development and Characterization of Microsatellite Markers for the Grain Amaranths [Abstract]
Single Nucleotide Polymorphism Identification, Characterization, and Linkage Mapping in Quinoa
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