Mongolian Libraries Assessment: A Collaboration Between Western Washington University and the American Center for Mongolian Studies

In November, 2015, Western Washington University (WWU) librarians Jenny Oleen and Gabe Gossett, the authors of this report, were invited to visit Ulaanbaatar in order to do a preliminary assessment of library services in the area and participate in a conference. The assessment was supported by the American Center for Mongolian Studies (ACMS) and WWU with the purpose of identifying ways in which there could be improved collaboration and development between Mongolian libraries, ACMS, and WWU (Western Libraries in particular). The librarians made numerous site visits over a two week period in Ulaanbaatar and the surrounding area to gather information about library facilities, collections, services, and, most importantly, their development goals in order to meet the information needs of Mongolians in the 21st Century. Near the end of their stay the librarians took part in a conference, hosted and co-sponsored by the Mongolian National University of Education, with over 200 library participants. This report includes an overview discussing general themes, a narrative describing issues and areas for development identified during library site visits and conference, and recommendations for partnerships

An Open and Shut Genome: The Dynamic Relationship between DNA Sequence, Transcription Factors, and Chromatin in S. cerevisiae

Transcription factors bind to regulatory regions to help activate or repress genes. Compaction of the genome into nucleosomes helps reduce the physical space that must be sampled to find in vivo targets, yet forces transcription factors and histones to compete for access to DNA. The equilibrium of this competition can be altered via post-translational modifications of the histone core and regulation of nucleosome position. I examined the differential contributions of DNA sequence, transcription factors, and chromatin to the regulation of gene expression in the model organism, Saccharomyces cerevisiae. While transcription factors bind DNA in a sequence specific fashion, the contribution of DNA in determining in vivo usage of consensus sequences is specific to the biological role of the transcription factor itself. Chromatin shows limited changes in different growth conditions, but localized changes surrounding transcription factor binding sites are evident. Chromatin stability appears to be regulated by the underlying DNA sequence of the genome and modified by active mechanisms such as post-translational modifications. Together, these processes establish stable chromatin in the body of genes and unstable chromatin at promoters. Depletion of nucleosomes is sufficient to alter transcription of 50% of the yeast genome, suggesting that chromatin plays a major role in regulating gene expression via regulating DNA accessibility to transcription factors as well as less direct effects, such as blocking assembly of the transcriptional machinery

A Novel Isoflurane Anesthesia Induction System for Raccoons

We developed a novel small-volume (24-L) conical-shaped isoflurane anesthesia induction chamber for use in a den chamber and tested it along with 3 conventional stand-alone induction chambers (2 clear acrylic plastic chambers and a cylindrical-shaped chamber) to determine utility for daily short-duration manipulations of captive raccoons (Procyon lotor). Although the conventional chambers were valuable, the majority of inductions were performed using the cone chamber in a pen setting. With the novel device, we were able to minimize the need for pre-anesthetic handling of animals and eliminate the need for injectable anesthesia agents. As a result, side effects normally associated with injectable agents were avoided. Mean anesthesia induction time using the cone chamber was 3.4 min (SD = 0.90). When used as designed, conventional chambers worked well, with induction times ranging from 2.7 min to 5.4 min. Because the stand-alone chambers were not reliant upon den chambers for use, they may provide greater utility for field work. The conical-shaped induction chamber, however, provides an option for safe short-duration anesthetization of captive raccoons and could perhaps be used with other species and in other research settings

AFLOW-SYM: Platform for the complete, automatic and self-consistent symmetry analysis of crystals

Determination of the symmetry profile of structures is a persistent challenge in materials science. Results often vary amongst standard packages, hindering autonomous materials development by requiring continuous user attention and educated guesses. Here, we present a robust procedure for evaluating the complete suite of symmetry properties, featuring various representations for the point-, factor-, space groups, site symmetries, and Wyckoff positions. The protocol determines a system-specific mapping tolerance that yields symmetry operations entirely commensurate with fundamental crystallographic principles. The self consistent tolerance characterizes the effective spatial resolution of the reported atomic positions. The approach is compared with the most used programs and is successfully validated against the space group information provided for over 54,000 entries in the Inorganic Crystal Structure Database. Subsequently, a complete symmetry analysis is applied to all 1.7$+$ million entries of the AFLOW data repository. The AFLOW-SYM package has been implemented in, and made available for, public use through the automated, $\textit{ab-initio}$ framework AFLOW.Comment: 24 pages, 6 figure

Greenwashing No More: The Case for Stronger Regulation of Environmental Marketing

Fraudulent and deceptive environmental claims in marketing (sometimes called “greenwashing”) are a persistent problem in the United States, despite nearly thirty years of efforts by the Federal Trade Commission (FTC) to prevent it. This Essay focuses on a recent trend in greenwashing - fraudulent “organic” claims for nonagricultural products, such as home goods and personal care products. We offer three recommendations. First, we suggest ways that the FTC can strengthen its oversight of “organic” claims for nonagricultural products and improve coordination with the USDA. Second, we argue for inclusion of guidelines for “organic” claims in the next revision of the FTC’s Guidelines for the Use of Environmental Marketing Claims (often referred to as the “Green Guides”), which the FTC is scheduled to revise in 2022. Finally, we assert that the FTC should formalize the Green Guides as binding regulations, rather than their current form as nonbinding interpretive guidance, as the USDA has done for the National Organic Program (NOP) regulations. This Essay concludes that more robust regulatory oversight of “organic” claims, together with efforts by the FTC to prevent other forms of greenwashing, will ultimately bolster demand for sustainable products and incentivize manufacturers to innovate to meet this demand

Hybrid dispersion laser scanner.

Laser scanning technology is one of the most integral parts of today's scientific research, manufacturing, defense, and biomedicine. In many applications, high-speed scanning capability is essential for scanning a large area in a short time and multi-dimensional sensing of moving objects and dynamical processes with fine temporal resolution. Unfortunately, conventional laser scanners are often too slow, resulting in limited precision and utility. Here we present a new type of laser scanner that offers ∼1,000 times higher scan rates than conventional state-of-the-art scanners. This method employs spatial dispersion of temporally stretched broadband optical pulses onto the target, enabling inertia-free laser scans at unprecedented scan rates of nearly 100 MHz at 800 nm. To show our scanner's broad utility, we use it to demonstrate unique and previously difficult-to-achieve capabilities in imaging, surface vibrometry, and flow cytometry at a record 2D raster scan rate of more than 100 kHz with 27,000 resolvable points

Inability of immunohistochemistry to predict clinical outcomes of endometrial cancer patients

Gossett DR, Alo P, Bristow RE, Galati M, Kyshtoobayeva A, Fruehauf J, Montz FJ. Inability of immunohistochemistry to predict clinical outcomes of endometrial cancer patients. Introduction: Despite optimal surgery, some patients with early endometrial carcinoma develop recurrence and die of disease. A number of immunohistochemical (IHC)-identified cell products (markers) have been proposed as predictors of recurrence. This study characterizes a large series of endometrial carcinomas with previously described markers as well as markers that have not been investigated in endometrial carcinoma. Patients and methods: Women who had undergone surgery for endometrial carcinoma were identified and specimens accessed. Tissue blocks were evaluated for ten IHC markers. Results were correlated with last known clinical status. Results: Mean follow-up was 43 months; complete data were available on 117 patients. Two women died of other causes; of the remaining 115, eight died of disease and six were alive with recurrence at last follow-up (12%). Vascular endothelial growth factor staining independently predicted recurrence and death. However, in multivariate analyses, only FIGO stage predicted outcome. Discussion: Our goal was to identify markers to predict which women with endometrial carcinoma were likely to have disease recurrence. We evaluated cell-cycle regulatory proteins, growth factors, hormone receptors, and angiogenic factors, but did not identify any marker that independently predicted outcome in multivariate analysis. This may reflect the few negative outcomes in our population.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72601/1/j.1048-891x.2004.014028.x.pd

Implementing Reference Statistics Collection Software at Multiple Library Service Points

Purpose– The purpose of this article is to explore the process of implementing LibAnswers, a reference statistics collection and analysis software, at multiple service points in an academic library and to outline the benefits of the process. Design/methodology/approach– The article outlines the process used to implement a statistics software, including initial testing, development of categories and terms used to define the question types, and training of library staff. Findings– Implementing a statistics program at multiple service points takes flexibility and patience. The biggest challenge faced was developing common terms to define what each desk did and how the statistics would be used. While this process was useful for implementing the software, it also resulted in unexpected organizational development opportunity as it allowed others to see and better understand the functions of different service points. A total commitment to the new program developed through training and the experiences of those who had used LibAnswers during a test period. Practical implications– LibAnswers, and similar statistics collection programs, are fairly new applications in the library field. While they have multiple uses for expanding and simplifying the collection of reference statistics, this article demonstrates that while implementing statistics software across multiple service points can be difficult, the process of putting it in place can produce numerous benefits. Originality/value– Much has been written on the implementation of reference statistics, but little has been written in detail about developing a library-wide statistics program. This article fills some of the gaps

Redundant Canonical and Noncanonical Caenorhabditis elegans p21-Activated Kinase Signaling Governs Distal Tip Cell Migrations

p21-activated kinases (Paks) are prominent mediators of Rac/Cdc42-dependent and -independent signaling and regulate signal transduction and cytoskeletal-based cell movements. We used the reproducible migrations of the Caenorhabditis elegans gonadal distal tip cells to show that two of the three nematode Pak proteins, MAX-2 and PAK-1, function redundantly in regulation of cell migration but are regulated by very different mechanisms. First, we suggest that MAX-2 requires CED-10/Rac function and thus functions canonically. Second, PIX-1 and GIT-1 function in the same role as PAK-1, and PAK-1 interaction with PIX-1 is required for PAK-1 activity; thus, PAK-1 functions noncanonically. The human Pak-Pix-Git complex is central to noncanonical Pak signaling and requires only modest Rac/CDC-42 input. Unlike the human complex, our results suggest that the C. elegans Pak-Pix-Git complex requires PAK-1 kinase domain activity. This study delineates signaling network relationships in this cell migration model, thus providing potential further mechanistic insights and an assessment of total Pak contribution to cell migration events