Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4

The small ubiquitin-like modifier (SUMO) can form polymeric chains that are important signals in cellular processes such as meiosis, genome maintenance and stress response. The SUMO-targeted ubiquitin ligase RNF4 engages with SUMO chains on linked substrates and catalyses their ubiquitination, which targets substrates for proteasomal degradation. Here we use a segmental labelling approach combined with solution nuclear magnetic resonance (NMR) spectroscopy and biochemical characterization to reveal how RNF4 manipulates the conformation of the SUMO chain, thereby facilitating optimal delivery of the distal SUMO domain for ubiquitin transfer

Characterizing late Quaternary lake-level variability in Lago de Tota, Colombian Andes, with CHIRP seismic stratigraphy

Geophysical analysis of lacustrine sediment stratigraphy at Lago de Tota (Tota), Boyaca, Colombia provided evidence for significant lake-level fluctuations through the late Quaternary and produced a record that potentially spans the last 60 ka. CHIRP data collected in 2015 from this large, high-elevation lake in the Eastern Cordillera of the northern hemisphere Colombian Andes reveal a series of off-lap and on-lap sequences in the upper ~ 20 m of the lake’s sediment column that indicate large amplitude changes in lake level. Because 14C dated sediment cores are only available for the upper 3 m of the sediment column, known Holocene sedimentation rates were extrapolated in order to assign preliminary ages to the off-lap and on-lap sequence boundaries below 3 m depth. These data suggest that lake levels at Tota were lower than present during marine isotope stage (MIS) 4 between 60 and 57 ka, relatively high during MIS 3 between 57 and 29 ka, fell to their lowest levels during MIS 2 between 29 and 14 ka, and gradually rose to the modern high stand through a series of transgressions during MIS 1 and the Holocene from ~ 14 ka to the present. These fluctuations are broadly consistent with trends observed in other lake-level reconstructions from the northern (in phase) and southern (out of phase) hemisphere Andes, possibly supporting the idea that millennial-to-orbital-scale South American hydroclimate variability is linked to shifts in the mean latitude of the intertropical convergence zone due to the influence of insolation- and ocean circulation-driven hemispheric temperature gradients during glacial/stadial and interglacial/interstadial events. Although additional geochronological data will be needed to better resolve the timing of the Tota lake-level changes and their relationships with other records, these preliminary results from Tota, as well as the presence of a thick (> 300 m) sedimentary archive, indicate that this site has significant potential to produce high-resolution, quantitative, paleo-hydroclimate data spanning much of the last 1 million years. Because geophysical surveys and long paleoclimate records from northern hemisphere South America are exceedingly rare, these data provide critical insight into regional hydroclimate trends through the Late Quaternary. Additional work, such as the collection of sediment cores spanning the depth interval represented in the CHIRP data, is required, however, in order to place firmer chronological constraints on the hypothesized timing of lake-level fluctuations at Tota and to investigate their paleo-hydroclimatic implications

Role of Haptoglobin in Polycystic Ovary Syndrome (PCOS), Obesity and Disorders of Glucose Tolerance in Premenopausal Women

alleles of the haptoglobin α–chain polymorphism reduce the anti-oxidant properties and increase the pro-inflammatory actions of this acute-phase protein in a gene-dosage fashion. We hypothesized that the haptoglobin polymorphism might contribute to the increased oxidative stress and low-grade chronic inflammation frequently associated with polycystic ovary syndrome, obesity, and abnormalities of glucose tolerance.<0.001), yet no association was found between obesity and haptoglobin genotypes. No differences were observed in haptoglobin levels or genotype frequencies depending on glucose tolerance. Fifty percent of the variation in serum haptoglobin concentrations was explained by the variability in serum C-reactive protein concentrations, BMI, insulin sensitivity and haptoglobin genotypes. alleles suggests that the anti-oxidant and anti-inflammatory properties of haptoglobin may be reduced in these patients

Human muscle-derived CLEC14A-positive cells regenerate muscle independent of PAX7

Skeletal muscle stem cells, called satellite cells and defined by the transcription factor PAX7, are responsible for postnatal muscle growth, homeostasis and regeneration. Attempts to utilize the regenerative potential of muscle stem cells for therapeutic purposes so far failed. We previously established the existence of human PAX7-positive cell colonies with high regenerative potential. We now identified PAX7-negative human muscle-derived cell colonies also positive for the myogenic markers desmin and MYF5. These include cells from a patient with a homozygous PAX7 c.86-1G > A mutation (PAX7null). Single cell and bulk transcriptome analysis show high intra- and inter-donor heterogeneity and reveal the endothelial cell marker CLEC14A to be highly expressed in PAX7null cells. All PAX7-negative cell populations, including PAX7null, form myofibers after transplantation into mice, and regenerate muscle after reinjury. Transplanted PAX7neg cells repopulate the satellite cell niche where they re-express PAX7, or, strikingly, CLEC14A. In conclusion, transplanted human cells do not depend on PAX7 for muscle regeneration

Late Quaternary hydroclimate variability in the Colombian Andes and the potential for deep sediment core recovery at Lago de Tota revealed through CHIRP and airgun-sourced seismic reflection data

A geophysical investigation of lacustrine stratigraphy at Lago de Tota (Tota), Boyaca, Colombia provides evidence for significant lake level fluctuations during the late Quaternary from ~60 ka to the present. CHIRP data collected in 2015 from this large, high-elevation lake in the Eastern Cordillera of the Northern Hemisphere (NH) Colombian Andes reveal a series of off-lap and on-lap sequences in the upper ~20 m of the lake’s sediment column that indicate large amplitude changes in lake level. These data, temporally constrained by 14C-dated sediment cores and extrapolated Holocene sedimentation rates, suggest that lake levels at Tota were lower than present during Marine Isotope Stage (MIS) 4 between 60 and 57 ka, relatively high during MIS 3 between 57 and 29 ka, fell to their lowest levels during MIS 2 between 29 and 14 ka, and gradually rose to the modern high-stand through a series of transgressions during MIS 1 and the Holocene from ~14 ka to the present. These fluctuations are broadly consistent with trends observed in other lake level reconstructions from the Northern (in phase) and Southern (out of phase) Hemisphere Andes, possibly supporting the idea that millennial-to-orbital-scale South American hydroclimate variability is linked to shifts in the mean latitude of the Intertropical Convergence Zone (ITCZ) due to the influence of insolation- and ocean circulation-driven hemispheric temperature gradients during glacial/stadial and interglacial/interstadial events. These results, as well as the presence of a thick (>300 m) sedimentary archive, indicate that Tota has significant potential to produce high-resolution, quantitative, paleo- hydroclimate data spanning much of the last 1 million years. Because geophysical surveys and long paleoclimate records from NH South America are exceedingly rare, these data provide critical insight into regional hydroclimate trends through the Late Quaternary

West Nile Virus Isolation in Human and Mosquitoes, Mexico

West Nile virus has been isolated for the first time in Mexico, from a sick person and from mosquitoes (Culex quinquefasciatus). Partial sequencing and analysis of the 2 isolates indicate that they are genetically similar to other recent isolates from northern Mexico and the western United States

Highlights from the Pierre Auger Observatory

The Pierre Auger Observatory is the world's largest cosmic ray observatory. Our current exposure reaches nearly 40,000 km$^2$ str and provides us with an unprecedented quality data set. The performance and stability of the detectors and their enhancements are described. Data analyses have led to a number of major breakthroughs. Among these we discuss the energy spectrum and the searches for large-scale anisotropies. We present analyses of our X$_{max}$ data and show how it can be interpreted in terms of mass composition. We also describe some new analyses that extract mass sensitive parameters from the 100% duty cycle SD data. A coherent interpretation of all these recent results opens new directions. The consequences regarding the cosmic ray composition and the properties of UHECR sources are briefly discussed.Comment: 9 pages, 12 figures, talk given at the 33rd International Cosmic Ray Conference, Rio de Janeiro 201

Wheat-barley hybridization – the last forty years

Abstract Several useful alien gene transfers have been reported from related species into wheat (Triticum aestivum), but very few publications have dealt with the development of wheat/barley (Hordeum vulgare) introgression lines. An overview is given here of wheat 9 barley hybridization over the last forty years, including the development of wheat 9 barley hybrids, and of addition and translocation lines with various barley cultivars. A short summary is also given of the wheat 9 barley hybrids produced with other Hordeum species. The meiotic pairing behaviour of wheat 9 barley hybrids is presented, with special regard to the detection of wheat– barley homoeologous pairing using the molecular cytogenetic technique GISH. The effect of in vitro multiplication on the genome composition of intergeneric hybrids is discussed, and the production and characterization of the latest wheat/barley translocation lines are presented. An overview of the agronomical traits (b-glucan content, earliness, salt tolerance, sprouting resistance, etc.) of the newly developed introgression lines is given. The exploitation and possible use of wheat/barley introgression lines for the most up-to-date molecular genetic studies (transcriptome analysis, sequencing of flow-sorted chromosomes) are also discussed

Colombia's cyberinfrastructure for biodiversity: Building data infrastructure in emerging countries to foster socioeconomic growth

Science and innovation are not a luxury but a prerequisite for social and economic development (Annan, 2003)