89 research outputs found
Sarcomas primarios de hueso: estudio por citometría estática mediante análisis digital de imagen
Se presenta un estudio morfométrico y densitométrico mediante análisis digital
de imagen de una serie de 50 tumores óseos malignos (32 osteosarcomas, 12 condrosarcomas
y 6 histiocitomas fibrosos malignos de hueso), con el fin de evaluar la utilidad
de la técnica para establecer el grado y el pronóstico de estas neoplasias. Las variables
morfométricas y la disposición de la cromatina guardan una estrecha relación con el grado
histológico (Spearman; p < 0,05) y muestran diferencias estadísticamente significativas
entre los tumores considerados de alto y bajo grado en base a su presentación y evolución
clínica. La ploidía estimada por densitometría óptica se comporta como un factor pronóstico
independiente en el análisis multivariante de la serie de tumores de alto grado (COX;
p < 0,001). La citometría estática, aunque más lenta e imprecisa que la citometría de flujo
en la determinación de los picos de ploidía, permite analizar el modelo de cromatina y
contribuye objetivamente al establecimiento del grado biológico de los tumores óseos.A densitometry and morphometric assessment by static image cytometry of
50 malignant bone tumors (32 osteosarcomas, 12 chondrosarcomas, and 6 malignant fibrous
histiocytomas of bone) was performed in order to assess the validity of the technic
in the pathobiological and prognostic evaluation of these neoplasms. A strong correlation
between the morphometric features, cromatine distribution and histologic grade was
found (Spearman; p < 0.05). These parameters are also statistically different for hight and
low grade tumors in clinical staging. The stimation of DNA ploidy by optic densitometry
is a significant prognostic factor in the multivariant analysis for the high grade tumors
(COX; p < 0.001). Although Flow cytometry is a better procedure to evaluate DNA ploidy
peaks, our study reveals static image cytometry as a useful technic for determine the
cromatine model and objective assessment of the biological grade of bone neoplasms
Electromiograph Parameters in Thedysfunctional Patient with Open Bite
INTRODUCTION: Open bite is an occlusal problem that appears in 0.5% of the population, and is more frequent in women. There is correlation between open bite and dysfunctional syndrome with TMJ disorders.
AIM OF THE STUDY: We attempted to analyze muscular parameters in patients with open bite treated with an occlusal splint by EMG recordings.
MATERIAL AND METHODS: Myotronics K6 electromiograph can provide information in the following situations: maximum force bite, lateral and protrusion movements, with and without an occlusal splint.
RESULTS: In maximum force bite with the occlusal splint more activity was found in the elevator muscles with a lack of dygastric activity. There was less EMG activity in protrusive and lateral
movements with occlusal splint.
CONCLUSION: With better support between the dental arches (occlusal splint) more activity was obtained in the elevators when biting, and when interferences were eliminated activity in the contralateral muscles was reduced
Poly[ADP-Ribose] Polymerase-1 Expression Is Related To Cold Ischemia, Acute Tubular Necrosis, and Delayed Renal Function In Kidney Transplantation
Cold ischemia time especially impacts on outcomes of expanded-criteria donor (ECD) transplantation. Ischemia-reperfusion (IR) injury produces excessive poly[ADP-Ribose] Polymerase-1 (PARP-1) activation. The present study explored the hypothesis that increased tubular expression of PARP-1 contributes to delayed renal function in suboptimal ECD kidney allografts and in non-ECD allografts that develop posttransplant acute tubular necrosis (ATN)
Lifestyles, environmental and phenotypic factors associated with lip cancer: a case–control study in southern Spain
Protective effect of policosanol on atherosclerotic lesions in rabbits with exogenous hypercholesterolemia
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Cutaneous metastasis as first clinical manifestation of signet ring cell gastric carcinoma
Automatic estimation procedure in partial linear model with functional data
Bandwidth selection, Cross-validation, Functional data, Partial linear regression, MSC 62G08, MSC 62G20,
Evolución de las lesiones hepáticas en pacientes con hepatitis crónica por VHC y respuesta clínica al interferón
Antiproliferative effect of topic hyaluronic acid gel. Study in gingival biopsies of patients with periodontal disease
Hyaluronic acid (HA) is the most abundant
glycosaminoglycan of high molecular weight in the
extracellular matrix of soft periodontal tissues. Our
group recently demonstrated an HA-induced reduction in
lymphoplasmocyte inflammatory infiltrate in periodontal
disease.
The objective of this study was to determine the
effect of an HA gel of high molecular weight on cell
proliferation, inflammation, and different periodontal
lesion parameters.
A double-blind clinical trial was conducted on the
effect of an HA gel on cell proliferation in gingival
biopsies from 28 patients with periodontal disease. A
split-mouth design was used, randomly applying the gel
to one quadrant and a placebo to the contralateral one. A
gingival biopsy was taken for histopathological and
immunohistochemical study, in order to determine the
expression of cell proliferation antigen Ki-67 and to
evaluate the inflammatory infiltrate.
HA gel treatment induced a significant reduction in
the proliferation index of the gingival epithelium, with
276 (range 234-317) Ki-67-positive cells per mm2 in
treated samples versus 514 (range 158-876) per mm2 in
controls (Mann-Whitney U test, p<0.003). In 13 patients,
the number of Ki-67-positive fibroblastic cells was
reduced by the treatment, whereas in 6 patients no
differences were found (global difference, p=0.12). In 10
patients, Ki-67-positive cells were decreased in chronic
inflammatory infiltrate present in the lamina propria,
whereas in 6 patients no differences were found (global
difference, p=0.054).
We conclude that high molecular-weight HA gel
reduces cell proliferation in epithelial cells such as
fibroblasts and lymphocytes, abates the inflammatory
process, and improves the periodontal lesion in patients
with chronic periodontitis
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