The radial abundance gradient of oxygen towards the Galactic anticentre

We present deep optical spectroscopy of eight HII regions located in the anticentre of the Milky Way. The spectra were obtained at the 10.4m GTC and 8.2m VLT. We determined Te([NII]) for all objects and Te([OIII]) for six of them. We also included in our analysis an additional sample of 13 inner-disc Galactic Hii regions from the literature that have excellent T_e determinations. We adopted the same methodology and atomic dataset to determine the physical conditions and ionic abundances for both samples. We also detected the CII and OII optical recombination lines in Sh 2-100, which enables determination of the abundance discrepancy factor for this object. We found that the slopes of the radial oxygen gradients defined by the HII regions from R_25 (= 11.5 kpc) to 17 kpc and those within R_25 are similar within the uncertainties, indicating the absence of flattening in the radial oxygen gradient in the outer Milky Way. In general, we found that the scatter of the O/H ratios of Hii regions is not substantially larger than the observational uncertainties. The largest possible local inhomogeneities of the oxygen abundances are of the order of 0.1 dex. We also found positive radial gradients in Te([O III]) and Te([N II]) across the Galactic disc. The shapes of these temperature gradients are similar and also consistent with the absence of flattening of the metallicity distribution in the outer Galactic disc.Comment: 20 pages, 11 figures. Accepted for publication in Monthly Notices of the Royal Astronomical Societ

Relations among low ionosphere parameters and high frequency radio wave absorption

Charged particle conductivities measured in the very low ionosphere at White Sands Missile Range, New Mexico, and Wallops Island, Virginia, are compared with atmospheric parameters and high frequency radio wave absorption measurements. Charged particle densities are derived from the conductivity data. Between 33 and 58 km, positive conductivity correlated well with neutral atmospheric temperature, with temperature coefficients as large as 4.6%/deg K. Good correlations were also found between HF radio wave absorption and negative conductivity at altitudes as low as 53 km, indicating that the day-to-day absorption variations were principally due to variations in electron loss rate

Does functional soil microbial diversity contribute to explain within-site plant beta-diversity in an alpine grassland and a <i>dehesa</i> meadow in Spain?

Questions: Once that the effects of hydrological and chemical soil properties have been accounted for, does soil microbial diversity contribute to explain change in plant community structure (i.e. within-site beta-diversity)? If so, at which spatial scale does microbial diversity operate? Location: La Mina in Moscosa Farm, Salamanca, western Spain (dehesa community) and Laguna Larga in the Urbión Peaks, Soria, central-northern Spain (alpine grassland). Methods: The abundance of vascular plant species, soil gram-negative microbial functional types and soil chemical properties (pH, available phosphorus, and extractable cations) were sampled at both sites, for which hydrological models were available. Redundancy analysis (RDA) was used to partition variation in plant community structure into hydrological, chemical and microbial components. Spatial filters, arranged in scalograms, were used to test for the spatial scales at which plant community structure change. Results: In the case of the dehesa the diversity of soil gram-negative microbes, weakly driven by soil pH, contributed to a small extent (adj-R2 = 2%) and at a relative medium spatial scale to explain change in plant community structure. The abundance of a few dehesa species, both annual (Trifolium dubium, Vulpia bromoides) and perennial (Poa bulbosa, Festuca ampla), was associated with either increasing or decreasing soil microbial diversity. In the alpine meadow the contribution was negligible. Conclusions: Microbial diversity can drive community structure, though in the hierarchy of environmental factors structuring communities it appears to rank lower than other soil factors. Still, microbial diversity appears to promote or restrain individual plant species. This paper aims to encourage future studies to use more comprehensive and insightful techniques to assess microbial diversity and to combine this with statistical approaches such as the one used here

Patient-Reported Outcomes Measurement Information System physical function correlates with Toronto Extremity Salvage Score in an orthopaedic oncology population

Background: The National Institute of Health\u27s Patient-Reported Outcomes Measurement Information System (PROMIS) uses computerised-adaptive testing to reduce survey burden and improve sensitivity. PROMIS is being used across medical and surgical disciplines but has not been studied in orthopaedic oncology. Questions/purposes: The aim of the study was to compare PROMIS measures with upper extremity (UE) and lower extremity (LE) Toronto Extremity Salvage Score (TESS) by assessing the following: (1) responder burden, (2) correlation between scores and (3) floor/ceiling effects. Patients and methods: This cross-sectional trial analysed all 97 adult patients treated surgically for a bone or soft tissue tumour at a tertiary institution between November 2015 and March 2016. TESS (UE or LE) and PROMIS (Physical Function, Pain Interference and Depression) surveys were administered preoperatively. Pearson correlations between each PROMIS domain and TESS were calculated, as were floor/ceiling effects of each outcome measure. Results: (1) Completion of three PROMIS questionnaires required a mean total of 16.8 (+/- 5.8 standard deviation) questions, compared with 31 and 32 questions for the LE and UE TESS questionnaires, respectively. (2) The PROMIS Physical Function scores demonstrated a strong positive correlation with the LE TESS (r = 0.84; 95% confidence interval [CI], 0.72-0.91; p \u3c 0.001) and moderate positive correlation with the UE TESS (r = 0.64; 95% CI, 0.34-0.83; p = 0.055). The PROMIS Depression scores demonstrated a weak negative correlation with both the LE TESS (r = -0.38; 95% CI, -0.61 to -0.10; p = 0.010) and with UE TESS (r = -0.38; 95% CI, -0.67 to -0.01; p = 0.055). The PROMIS Pain Interference scores demonstrated a strong negative correlation with the LE TESS (r = -0.71; 95% CI, -0.83 to -0.52; p \u3c 0.001) and a moderate negative correlation with the UE TESS (r = -0.62; 95% CI, -0.81 to -0.30; p = 0.001). (3) The UE TESS had a range of scores from 16 to 100 with a 27% ceiling effect and no floor effect, and the LE TESS had a range from 10 to 98 with no floor or ceiling effect. There was no floor or ceiling effect for any PROMIS measures. Conclusions: In an orthopaedic oncology population, the PROMIS Physical Function and Pain Interference scores correlate with the TESS and have the benefit of reduced survey burden and ceiling effect. The PROMIS Depression scores may provide additional information regarding patient outcomes not captured by the TESS. Level of Evidence: Level III. The translational potential of this article: Patient reported outcome measures asses patients\u27 symptoms, function and health-related quality of life and are designed to capture more clinical information than can be gathered by objective medial testing alone. As reimbursements and the understanding of patient outcomes are becoming tied to performance on PROMIS measures, it is an important step to establish how PROMIS measures correlate and compare to traditional legacy measures

Influencing factors in energy use of housing blocks: a new methodology, based on clustering and energy simulations, for decision making in energy refurbishment projects

In recent years, big efforts have been dedicated to identify which are the factors with highest influence in the energy consumption of residential buildings. These factors include aspects such as weather dependence, user behaviour, socio-economic situation, type of the energy installations and typology of buildings. The high number of factors increases the complexity of analysis and leads to a lack of confidence in the results of the energy simulation analysis. This fact grows when we move one step up and perform global analysis of blocks of buildings. The aim of this study is to report a new methodology for the assessment of the energy performance of large groups of buildings when considering the real use of energy. We combine two clustering methods, Generative Topographic Mapping and&nbsp;k-means, to obtain reference dwellings that can be considered as representative of the different energy patterns and energy systems of the neighbourhood. Then, simulation of energy demand and indoor temperature against the monitored comfort conditions in a short period is performed to obtain end use load disaggregation. This methodology was applied in a district at Terrassa City (Spain), and six reference dwellings were selected. Results showed that the method was able to identify the main patterns and provide occupants with feasible recommendations so that they can make required decisions at neighbourhood level. Moreover, given that the proposed method is based on the comparison with similar buildings, it could motivate building occupants to implement community improvement actions, as well as to modify their behaviour

Tethering polypeptides through bifunctional PEG cross-linking agents to probe protein function: application to ATP synthase.

Chemical crosslinking mediated by short bifunctional reagents has been widely used for determining physical relationships among polypeptides in multisubunit proteins, but less often for functional studies. Here we introduce the approach of tethering polypeptides by using bifunctional reagents containing a lengthy, flexible PEG linker as a form of crosslinking especially suited to functional analyses. The rotary molecular motor ATP synthase was used as a model subject. Single cysteine residues were introduced into selected positions of ATP synthase epsilon subunit, a component of the rotor subcomplex of the enzyme, and the unrelated maltose binding protein (MBP), then the two purified recombinant proteins were crosslinked by means of a dimaleimido-PEG cross-linking agent. Following purification, the epsilon-PEG-MBP was incorporated into membrane-bound ATP synthase by reconstitution with epsilon-depleted F(1)-ATPase and membrane vesicles that had been stripped of endogenous F(1). ATP synthase reconstituted using epsilon-PEG-MBP had reduced ATP hydrolytic activity that was uncoupled from the pumping of H(+), indicating the physical blockage of rotation of the gammaepsilonc(10) rotor by the conjugated MBP, whereas enzyme reconstituted with epsilon-PEG was normal. These results directly demonstrate the feasibility of studying mechanistic features of molecular motors through PEG-based conjugation of unrelated proteins. Since tethering polypeptides provides a means of maintaining proximity without directly specifying or modifying interactions, application of the general method to other types of protein functional studies is envisioned

Design methodology for low cost tubular digesters

The aim of this paper is to present a novel, universal, methodology for the design of low cost tubular digesters. This method improves on the established methodology by avoiding assumptions that tend to reduce the final&nbsp;hydraulic retention time&nbsp;(HRT) of digesters once installed. This work recommends designing the digester using trench cross-sectional area and proposes an optimization of the trench dimensions with respect to the angle of the walls and the relationship between the length of the biogas bell and the top width of the trench. The influence of the biogas pressure is considered. A simple geometrical analysis is presented that, by&nbsp;parameterization, can be applied in a wide range of situations

36 degree step size of proton-driven c-ring rotation in FoF1-ATP synthase

Synthesis of the biological "energy currency molecule" adenosine triphosphate ATP is accomplished by FoF1-ATP synthase. In the plasma membrane of Escherichia coli, proton-driven rotation of a ring of 10 c subunits in the Fo motor powers catalysis in the F1 motor. While F1 uses 120 degree stepping, Fo models predict a step-by-step rotation of c subunits 36 degree at a time, which is here demonstrated by single-molecule fluorescence resonance energy transfer.Comment: 8 pages, 1 figur