84 research outputs found
Leaf Eh and pH: A Novel Indicator of Plant Stress. Spatial, Temporal and Genotypic Variability in Rice (Oryza sativa L.)
A wealth of knowledge has been published in the last decade on redox regulations in plants. However, these works remained largely at cellular and organelle levels. Simple indicators of oxidative stress at the plant level are still missing. We developed a method for direct measurement of leaf Eh and pH, which revealed spatial, temporal, and genotypic variations in rice. Eh (redox potential) and Eh@pH7 (redox potential corrected to pH 7) of the last fully expanded leaf decreased after sunrise. Leaf Eh was high in the youngest leaf and in the oldest leaves, and minimum for the last fully expanded leaf. Leaf pH decreased from youngest to oldest leaves. The same gradients in Eh-pH were measured for various varieties, hydric conditions, and cropping seasons. Rice varieties differed in Eh, pH, and/or Eh@pH7. Leaf Eh increases and leaf pH decreases with plant age. These patterns and dynamics in leaf Eh-pH are in accordance with the pattern and dynamics of disease infections. Leaf Eh-pH can bring new insight on redox processes at plant level and is proposed as a novel indicator of plant stress/health. It could be used by agronomists, breeders, and pathologists to accelerate the development of crop cultivation methods leading to agroecological crop protection
Air-sea gas transfer velocity estimates from the Jason-1 and TOPEX altimeters : prospects for a long-term global time series
Author Posting. © Elsevier B.V., 2006. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Journal of Marine Systems 66 (2007): 173-181, doi:10.1016/j.jmarsys.2006.03.020.Estimation of global and regional air–sea fluxes of climatically important gases is a key goal of current climate research programs. Gas transfer velocities needed to compute these fluxes can be estimated by combining altimeter-derived mean square slope with an empirical relation between transfer velocity and mean square slope derived from field measurements of gas fluxes and small-scale wave spectra [Frew, N.M., Bock, E.J., Schimpf, U., Hara, T., Hauβecker, H., Edson, J.B., McGillis, W.R., Nelson, R.K., McKenna, S.P., Uz, B.M., Jähne, B., 2004. Air–sea gas transfer: Its dependence on wind stress, small-scale roughness and surface films, J. Geophys. Res., 109, C08S17, doi: 10.1029/2003JC002131.]. We previously reported initial results from a dual-frequency (Ku- and C-band) altimeter algorithm [Glover, D.M., Frew, N.M., McCue, S.J., Bock, E.J., 2002. A Multi-year Time Series of Global Gas Transfer Velocity from the TOPEX Dual Frequency, Normalized Radar Backscatter Algorithm, In: Gas Transfer at Water Surfaces, editors: Donelan, M., Drennan, W., Saltzman, E., and Wanninkhof, R., Geophysical Monograph 127, American Geophysical Union, Washington, DC, 325–331.] for estimating the air–sea gas transfer velocity (k) from the mean square slope of short wind waves (40–100 rad/m) and derived a 6-year time series of global transfer velocities based on TOPEX observations. Since the launch of the follow-on altimeter Jason-1 in December 2001 and commencement of the TOPEX/Jason-1 Tandem Mission, we have extended this time series to 12 years, with improvements to the model parameters used in our algorithm and using the latest corrected data releases. The prospect of deriving multi-year and interdecadal time series of gas transfer velocity from TOPEX, Jason-1 and follow-on altimeter missions depends on precise intercalibration of the normalized backscatter. During the Tandem Mission collinear phase, both satellites followed identical orbits with a mere 73-s time separation. The resulting collocated, near-coincident normalized radar backscatter (σ°) data from both altimeters present a unique opportunity to intercalibrate the two instruments, compare derived fields of transfer velocity and estimate the precision of the algorithm. Initial results suggest that the monthly gas transfer velocity fields generated from the two altimeters are very similar. Comparison of along-track Ku-band and C-band σ° during the collinear phase indicates that observed discrepancies are due primarily to small offsets between TOPEX and Jason-1 σ°. The Jason-1 k values have an apparent bias of + 4% relative to TOPEX, while the precision estimated from the two observation sets is 5–7% and scales with k. The resultant long-term, global, mean k is 16 cm/h.We gratefully acknowledge funding support from NASA under grant NAGW–2431 and JPL
contract 961425
The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition
It has been proposed that ATR kinase senses the completion of DNA replication to initiate the S/G2 transition. In contrast to this model, we show here that the TRESLIN-MTBP complex prevents a premature entry into G2 from early S-phase independently of ATR/CHK1 kinases. TRESLIN-MTBP acts transiently at pre-replication complexes (preRCs) to initiate origin firing and is released after the subsequent recruitment of CDC45. This dynamic behavior of TRESLIN-MTBP implements a monitoring system that checks the activation of replication forks and senses the rate of origin firing to prevent the entry into G2. This system detects the decline in the number of origins of replication that naturally occurs in very late S, which is the signature that cells use to determine the completion of DNA replication and permit the S/G2 transition. Our work introduces TRESLIN-MTBP as a key player in cell-cycle control independent of canonical checkpoints
A new approach to estimation of global air-sea gas transfer velocity fields using dual-frequency altimeter backscatter
Author Posting. © American Geophysical Union, 2007. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 112 (2007): C11003, doi:10.1029/2006JC003819.A new approach to estimating air-sea gas transfer velocities based on normalized backscatter from the dual-frequency TOPEX and Jason-1 altimeters is described. The differential scattering of Ku-band (13.6 GHz) and C-band (5.3 GHz) microwave pulses is used to isolate the contribution of small-scale waves to mean square slope and gas transfer. Mean square slope is derived for the nominal wave number range 40–100 rad m−1 by differencing mean square slope estimates computed from the normalized backscatter in each band, using a simple geometric optics model. Model parameters for calculating the differenced mean square slope over this wave number range are optimized using in situ optical slope measurements. An empirical relation between gas transfer velocity and mean square slope, also based on field measurements, is then used to derive gas transfer velocities. Initial results demonstrate that the calculated transfer velocities exhibit magnitudes and a dynamic range which are generally consistent with existing field measurements. The new algorithm is used to construct monthly global maps of gas transfer velocity and to illustrate seasonal transfer velocity variations over a 1-year period. The measurement precision estimated from >106 duplicate observations of the sea surface by TOPEX and Jason-1 altimeters orbiting in tandem is better than 10%. The estimated overall uncertainty of the method is ±30%. The long-term global, area-weighted, Schmidt number corrected, mean gas transfer velocity is 13.7 ± 4.1 cm h−1. The new approach, based on surface roughness, represents a potential alternative to commonly used parameterizations based on wind speed.Financial support for this research from the
National Aeronautics and Space Administration through Jet Propulsion
Laboratory contract 961425 and the NOAA Global Carbon Cycle Program
under grant NA16GP2918, Office of Global Programs is gratefully acknowledged
The Testing of Correlation between the Biophysical States in the Host Tissues and the Susceptibility of Cereals to Powdery Mildew (Erysiphe graminis DC.)
Effect of different levels of calcium nitrate and potassium dihydrogen phosphate on powdery mildew ( Erysiphe graminis
The Influence of pH of Barley Tissues on the Symptoms Caused by Powdery Mildew (Erysiphe graminis DC)
- …