93 research outputs found
Comportamiento hidromorfológico de los microambientes de campos abandonados con lluvias intensas: experiencias en el Valle de Aisa (Pirineo Aragonés)
[Resumen] A partir de pruebas de simulación de lluvia se estudia la respuesta hidromorfológica de diferentes microambientes de campos abandonados en pendiente del Pirineo (valle de Aísa). Los resultados muestran pérdidas de suelo moderadas en los microambientes con buen cubrimiento vegetal, tanto si es de matorral como de herbáceas, y relativamente más elevadas en el enlosado de piedras. La disminución de la vegetación implica incrementos considerables en el coeficiente de escorrentía y en las pérdidas de suelo.[Abstract] Using a rainfall simulator the authors have studied the hydromorphological response of different micro-environments of abandoned sloping fields (Aísa Valley, Pyrenees). The results show moderate sediment losses in microenvironments with a good vegetable cover of shrub or meadows. The erosion is more important in fields with a stone pavement. The diminution of the vegetation involves considerable increments of the runoff coefficients and the erosion rates
Fire-related debris flows in the Iberian Range, Spain
38 páginas, 2 tablas, 11 figuras[EN] Debris flows occurred three weeks after a wildfire in August 1986 in the Najerilla River valley in the Iberian Range, northern Spain. The flows were triggered by a brief, intense rainstorm (approximately 25 mm h− 1 over 15 min) in a small area with steep slopes covered by a thick colluvium of quartzite clasts. This storm resulted in the development of several unconfined hillslope debris flows and the formation of an alluvial fan at the mouth of the Pítare stream, which partially blocked the Najerilla River. We analysed the conditions that led to the development of the debris flows, and estimated the rainfall threshold for the debris flows to occur as well as the total volume of mobilised sediment. Four factors contributed to the debris flows: (i) the occurrence of a rainstorm three weeks after a wildfire, which had removed the plant cover from the soil; (ii) the steep slopes in the area (> 30°), which were the most affected by debris flows; (iii) the presence of quartzite scarps on the hillslopes, which favoured the development of a ‘firehose effect’ involving channelised surface runoff; and (iv) the low plasticity index values of the fine material of the colluvium (indices of 7 to 8), which enabled rapid liquefaction. Estimates of rainfall intensity derived from the estimated peak flow in the Pítare stream suggests that around 80 mm of rainfall fell in approximately 15 min, although this is clearly an overestimated value given the high proportion of sediment load transported during the peak flow. Various equations estimated a rainfall-threshold of approximately 25 mm h− 1 considering a concentration time of 15 min. The total sediment transported by the debris flows was 10,500 m3 (15,750 Mg, 6800 Mg km− 2), and the Pítare stream alone transported a minimum of 4000 m3 (6000 Mg, 2500 Mg km− 2). These results suggest that the rainfall threshold for initiating debris flows decreases following a wildfire, such that an ordinary rainstorm is able to trigger a severe erosion and sediment transport event. Given the absence of fresh landslide scars on the hillslopes, the origin of the fire-related debris flows in the Najerilla River valley appears to have been directly linked to increased rates of overland flow having a greater effect than infiltration for triggering debris flows.Support for this research was provided by the projects PROBASE (CGL2006-11619/HID) and INDICA (CGL2011-27753-C02-01 and CGL2011-27753-C02-02), which was funded by the Spanish Ministry of Education and Science, and ACQWA (FP7-ENV-2007-1-212250), which was financed by the European Commission. Noemí Lana-Renault was the recipient of a research contract (Programme “Juan de la Cierva”, Human Resources Mobility, funded by the Spanish Ministry of Economy and Competitivity). The authors gratefully acknowledge Dr. Susan Canon, Dr. Francisco Gutiérrez-Santolalla and an anonymous reviewer for their comments and suggestions, which significantly helped to improve this paper.Peer reviewe
Osteointegración de implantes de titanio con superficies activas. Un estudio proteómico
Titanium is a biomaterial largely used on dental implant
manufacturing. However, as a consequence of its
intrinsically low bioactivity, the development of distinct
superficial treatments in order to enhance its osseointegration
properties is being studied. In this sense,
the use of titanium implants with a higher level of
roughness has been broadened, recurring to the application
of sand-blasted acid-etched
surface treatments. In this
article, a study of two distinct
titanium surface treatments
has been carried out, regarding
the physico-chemical
properties (roughness, hydrophilicity
and chemical composition)
of each, as well as the
pattern of adhered proteins onto
each surface (proteomic study).
Hence, mass spectrometry
analysis allowed the detection
of 2 18 d istinct a dsorbed p roteins,
being 37 of those related
to bone regenerative processes
and dental implant integration.
Moreover, using differential
quantification between associated proteins, comparing
surfaces, it was observable a greater affinity of
APOE, ANT3 and PROC proteins to the treated surface,
directly linked to the bone regenerative process.
On the other hand, the treated surface displays lower
affinity of CO3 protein. The variations between the
adsorbed protein profiles could be an explanation for
distinct in vivo outcomes.El titanio es un biomaterial ampliamente empleado en
la fabricación de implantes dentales, sin embargo, como
consecuencia de su baja bioactividad se han desarrollado
distintos tratamientos superficiales buscando
una mejora en su capacidad de osteintegración. De esta
forma, se ha extendido el uso de implantes de titanio
con un mayor grado de rugosidad gracias a la aplicación
de un tratamiento de granallado, al que le sigue
un tratamiento de ataque ácido. En este artículo se ha
llevado a cabo un estudio de discos de titanio con dos
tipos de superficie: sin tratamiento alguno y con tratamiento
de granallado más ataque ácido. El estudio
reveló diferencias físico-químicas (rugosidad, hidrofilia
y composición química) tras la aplicación del tratamiento
superficial, pero también en cuanto al perfil
de proteínas adheridas a cada superficie (estudio
proteómico). Así, la espectrometría de masas permitió
la caracterización de las proteínas adsorbidas en ambos
tipos de superficies. El análisis permitió la identificación
de 218 proteínas distintas, pudiendo relacionar
37 de ellas con el proceso de regeneración ósea y en
consecuencia con la osteointegración de un implante
dental. Además, tras la cuantificación diferencial entre
proteínas asociadas, antes y después de aplicar el tratamiento
superficial mencionado, se observó que tras
su aplicación se producía un aumento en la afinidad
de las proteínas APOE, ANT3 y PROC, directamente
relacionadas con el proceso de regeneración ósea. Por
el contrario, la proteína CO3 se adhería a esta superficie
en menor proporción. Estas variaciones de los perfiles
de proteínas podrían explicar la diferencia encontrada
en la respuesta de las distintas superficies al ser
caracterizadas en cuanto a su comportamiento in vivo
Development and characterisation of strontium-doped sol-gel coatings to optimise the initial bone regeneration processes
Strontium plays an important role in bone regeneration; it promotes the differentiation and maturation of osteoblasts and inhibits the activity of osteoclasts. Our principal objective in this study was to formulate new organic-inorganic hybrid sol-gel coatings applied to titanium discs. These coatings were functionalised with different amounts of SrCl2 and examined using in vitro tests and proteomics. The chemical and morphological characteristics of obtained coatings were scrutinised. The in vitro evaluation using the MC3T3-E1 osteoblasts and RAW264.7 macrophages showed the osteogenic and anti-inflammatory effects of strontium doping. The proteomic assay identified 111 different proteins adhering to the coatings. Six of these proteins reduced their adhesion affinity as a result of Sr-doping, whereas 40 showed increased affinity. Moreover, the proteomic analysis revealed osteogenic and anti-inflammatory properties of these biomaterials. The analysis also showed increased adhesion of proteins related to the coagulation system. We can conclude that proteomic methods are invaluable in developing new biomaterials and represent an important tool for predicting the biocompatibility of dental implants
Insight into the antibacterial mechanism of Cu-enriched sol–gel coatings employing proteomics
Advanced antibacterial biomaterials can help reduce the severe consequences of infections. Using copper compounds is an excellent option to achieve this goal; they offer a combination of regenerative and antimicrobial functions. In this study, new CuCl2-doped sol–gel coatings were developed and physicochemically characterised. Their osteogenic and inflammatory responses were tested in vitro using human osteoblasts and THP-1 macrophages. Their antibacterial effect was evaluated using Escherichia coli and Staphylococcus aureus. The Cu influence on the adsorption of human serum proteins was analysed employing proteomics. The materials released Cu2+ and were not cytotoxic. The osteoblasts in contact with these materials showed an increased ALP, BMP2 and OCN gene expression. THP-1 showed an increase in pro-inflammatory markers related to M1 polarization. Moreover, Cu-doped coatings displayed a potent antibacterial behaviour against E. coli and S. aureus. The copper ions affected the adsorption of proteins related to immunity, coagulation, angiogenesis, fibrinolysis, and osteogenesis. Interestingly, the coatings had increased affinity to proteins with antibacterial functions and proteins linked to the complement system activation that can lead to direct bacterial killing via large pore-forming complexes. These results contribute to our understanding of the antibacterial mechanisms of Cu-biomaterials and their interaction with biological systems.This work was supported by Ministerio Ciencia e Innovación [PID2020-113092RB-C21], Generalitat Valenciana [APOSTD/2020/036, PROMETEO/2020/069], Universitat Jaume I [UJI-B2021-25] and Basque Government [MARSA21/07]. The authors would like to thank Raquel Oliver, José Ortega and Iraide Escobés for their valuable technical assistance and GMI-Ilerimplant for making the titanium discs
Proteome analysis of human serum proteins adsorbed onto different titanium surfaces used in dental implants
Titanium dental implants are commonly used due to their biocompatibility and biochemical properties; blasted acid-etched Ti is used more frequently than smooth Ti surfaces. In this study, physico-chemical characterisation revealed important differences in roughness, chemical composition and hydrophilicity, but no differences were found in cellular in vitro studies (proliferation and mineralization). However, the deposition of proteins onto the implant surface might affect in vivo osseointegration. To test that hypothesis, protein layers formed on discs of both surface type after incubation with human serum were analysed. Using mass spectrometry (LC/MS/MS), 218 proteins were identified, 30 of which were associated with bone metabolism. Interestingly, Apo E, antithrombin and protein C adsorbed mostly onto blasted and acid-etched Ti, whereas the proteins of the complement system (C3) were found predominantly on smooth Ti surfaces. These results suggest that physico-chemical characteristics could be responsible for the differences observed in the adsorbed protein layer.This work was supported by Ministerio de Economía y Competitividad (MINECO) [MAT 2014-51918-C2-2-R], Universidad de Castellón [P11B2014-19], Plan de Promoción de la Investigación de la Universidad Jaume I under grant [Predoc/2014/25] and Generalitat Valenciana under grant [Grisolia/2014/016].
The authors would like to thank Antonio Coso and Jaime Franco (GMI-Ilerimplant) for their inestimable contribution to this study, and Iraida Escobes (CIC bioGUNE) for her valuable technical assistance
The effect of strontium incorporation into sol-gel biomaterials on their protein adsorption and cell interactions
[EN] It is known strontium can both inhibit the osteoclast formation and stimulate the osteoblast maturation, so biomaterials containing this element can favour bone structure stabilisation. The addition of Sr to biomaterials could affect their interactions with proteins and cells. Here, a silica-hybrid sol-gel network doped with different amounts of SrCl2 and applied as coatings on titanium discs was examined. in vitro analysis was performed to determine the potential effect of Sr in the coatings, showing enhanced gene expression of osteogenic markers (alkaline phosphatase and transforming growth factor-beta) in MC3T3-E1 incubated with Sr-doped biomaterials. The examination of inflammatory markers (tumour necrosis factor-alpha and interleukin 10) in RAW 264.7 macrophages revealed an anti-inflammatory potential of these materials. Proteins adsorbed onto the coatings incubated with human serum (3 h at 37 degrees C) were also analysed; mass spectrometry was used to characterise the proteins adhering to materials with different Sr content. Adding Sr to the coatings increased their affinity to APOE and VTNC proteins (associated with anti-inflammatory and osteogenic functions). Moreover, the proteins involved in coagulation processes, such as prothrombin, were more abundant on the coatings containing Sr than on the base sol-gel surfaces. Correlations between gene expression and proteomic results were also examined.This work was supported by MINECO (MAT2017-86043-R); Universitat Jaume I (grant numbers Predoc/2014/25, UJI-B2017-37); Basque Government (grant numbers IT611-13, Predoc/2016/1/0141), and University of the Basque Country (UFI11/56). Authors would like to thank Antonio Coso and Jaime Franco (GMI-Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver, Jose Ortega (UJI), and Iraide Escobes (CIC bioGUNE) for their valuable technical assistance.Romero-Gavilán, F.; Araújo-Gomes, N.; García-Arnáez, I.; Martínez-Ramos, C.; Elortza, F.; Azkargorta, M.; Iloro, I.... (2019). The effect of strontium incorporation into sol-gel biomaterials on their protein adsorption and cell interactions. Colloids and Surfaces B Biointerfaces. 174:9-16. https://doi.org/10.1016/j.colsurfb.2018.10.075S91617
Complex effects of Mg-biomaterials on the osteoblast cell machinery: A proteomic study
The cell–biomaterial interface is highly complex; thousands of molecules and many processes participate in its formation. Growing demand for improved biomaterials has highlighted the need to understand the structure and functions of this interface. Proteomic methods offer a viable alternative to the traditional in vitro techniques for analyzing such systems. Magnesium is a promoter of cell adhesion and osteogenesis. Here, we used the LC-MS/MS to compare the protein expression profiles of human osteoblasts (HOb) exposed to sol-gel coatings without (MT) and with Mg (MT1.5Mg) for 1, 3, and 7 days. PANTHER, DAVID, and IPA databases were employed for protein identification and data analysis. Confocal microscopy and gene expression analysis were used for further characterization. Exposure to MT1.5Mg increased the HOb cell area and the expression of SP7, RUNX2, IBP3, COL3A1, MXRA8, and FBN1 genes. Proteomic analysis showed that MT1.5Mg affected the early osteoblast maturation (PI3/AKT, mTOR, ERK/MAPK), insulin metabolism, cell adhesion (integrin, FAK, actin cytoskeleton regulation) and oxidative stress pathways. Thus, the effects of Mg on cell adhesion and osteogenesis are rather complex, affecting several pathways rather than single processes. Our analysis also confirms the potential of proteomics in biomaterial characterization, showing a good correlation with in vitro results.Funding for open access charge: CRUE-Universitat Jaume IThis work was supported by Ministerio Ciencia e Innovación [PID2020-113092RB-C21]; Generalitat Valenciana [GRISOLIAP/2018/091, APOSTD/2020/036, PROMETEO/2020/069]; Universitat Jaume I [UJI-B2021-25]; and Basque Government [PRE_2017_2_0044]. The authors would like to thank Raquel Oliver, José Ortega, José Miguel Pedra and Iraide Escobés for their valuable technical assistance and Antonio Coso (GMI-Ilerimplant) for making the titanium discs
Proteomic analysis of calcium-enriched sol-gel biomaterials
[EN] Calcium is an element widely used in the development of biomaterials for bone tissue engineering as it plays important roles in bone metabolism and blood coagulation. The Ca ions can condition the microenvironment at the tissue-material interface, affecting the protein deposition process and cell responses. The aim of this study was to analyze the changes in the patterns of protein adsorption on the silica hybrid biomaterials supplemented with different amounts of CaCl2, which can function as release vehicles. This characterization was carried out by incubating the Ca-biomaterials with human serum. LC-MS/MS analysis was used to characterize the adsorbed protein layers and compile a list of proteins whose affinity for the surfaces might depend on the CaCl2 content. The attachment of pro- and anti-clotting proteins, such as THRB, ANT3, and PROC, increased significantly on the Ca-materials. Similarly, VTNC and APOE, proteins directly involved on osteogenic processes, attached preferentially to these surfaces. To assess correlations with the proteomic data, these formulations were tested in vitro regarding their osteogenic and inflammatory potential, employing MC3T3-E1 and RAW 264.7 cell lines, respectively. The results confirmed a Ca dose-dependent osteogenic and inflammatory behavior of the materials employed, in accordance with the protein attachment patterns.This work was supported by MINECO [MAT2017-86043-R]; Universitat Jaume I [Grant numbers Predoc/2014/25, UJI-B2017-37]; Basque Government [Grant numbers IT611-13, Predoc/2016/1/0141]; University of the Basque Country [Grant number UFI11/56]. CIC bioGUNE is supported by Basque Department of Industry, Tourism and Trade (Etortek and Elkartek programs), the Innovation Technology Department of the Bizkaia County; The ProteoRed-ISCIII (Grant PRB3 IPT17/0019); CIBERehd Network, and Severo Ochoa Grant (SEV-2016-0644). Authors would like to thank Antonio Coso and Jaime Franco (GMI-Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver, Jose Ortega (UJI) and Iraide Escobes (CIC bioGUNE) for their valuable technical assistance.Romero-Gavilán, F.; Araújo-Gomes, N.; Cerqueira, A.; García-Arnáez, I.; Martínez-Ramos, C.; Azkargorta, M.; Iloro, I.... (2019). Proteomic analysis of calcium-enriched sol-gel biomaterials. JBIC Journal of Biological Inorganic Chemistry. 24(4):563-574. https://doi.org/10.1007/s00775-019-01662-5S563574244Berridge MJ, Bootman MD, Lipp P (1998) Calcium—a life and death signal. Nature 395:645–648. https://doi.org/10.1038/27094Hoppe A, Güldal NS, Boccaccini AR (2011) A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials 32:2757–2774. https://doi.org/10.1016/j.biomaterials.2011.01.004Flynn A (2003) The role of dietary calcium in bone health. Proc Nutr Soc 62:851–858. https://doi.org/10.1079/PNS2003301Marie PJ (2010) The calcium-sensing receptor in bone cells: a potential therapeutic target in osteoporosis. Bone 46:571–576. https://doi.org/10.1016/j.bone.2009.07.082Honda Y, Fitzsimmons RJ, Baylink DJ, Mohan S (1995) Effects of extracellular calcium on insulin-like growth factor II in human bone cells. J Bone Miner Res 10:1660–1665. https://doi.org/10.1002/jbmr.5650101108Koori K, Maeda H, Fujii S et al (2014) The roles of calcium-sensing receptor and calcium channel in osteogenic differentiation of undifferentiated periodontal ligament cells. Cell Tissue Res 357:707–718. https://doi.org/10.1007/s00441-014-1918-5Habibovic P, Barralet JE (2011) Bioinorganics and biomaterials: bone repair. Acta Biomater 32:3013–3026. https://doi.org/10.1016/j.actbio.2011.03.027Oshiro Junior J, Paiva Abuçafy M, Berbel Manaia E et al (2016) Drug delivery systems obtained from silica based organic-inorganic hybrids. Polymers (Basel) 8:91. https://doi.org/10.3390/polym8040091Jones JR (2015) Reprint of: review of bioactive glass: from hench to hybrids. Acta Biomater 23:S53–S82. https://doi.org/10.1016/j.actbio.2015.07.019Romero-Gavilán F, Barros-Silva S, García-Cañadas J et al (2016) Control of the degradation of silica sol-gel hybrid coatings for metal implants prepared by the triple combination of alkoxysilanes. J Non Cryst Solids 453:66–73. https://doi.org/10.1016/j.jnoncrysol.2016.09.026Martínez-Ibáñez M, Juan-Díaz MJ, Lara-Saez I et al (2016) Biological characterization of a new silicon based coating developed for dental implants. J Mater Sci Mater Med 27:80. https://doi.org/10.1007/s10856-016-5690-9Martínez-Ibáñez M, Murthy NS, Mao Y et al (2018) Enhancement of plasma protein adsorption and osteogenesis of hMSCs by functionalized siloxane coatings for titanium implants. J Biomed Mater Res Part B Appl Biomater 106:1138–1147. https://doi.org/10.1002/jbm.b.33889Salinas AJ, Merino JM, Babonneau F et al (2007) Microstructure and Macroscopic Properties of Bioactive CaO–SiO2–PDMS Hybrids. J Biomed Mater Res B Appl Biomater 81B:274–282. https://doi.org/10.1002/jbm.b.30663Almeida JC, Wacha A, Gomes PS et al (2016) A biocompatible hybrid material with simultaneous calcium and strontium release capability for bone tissue repair. Mater Sci Eng, C 62:429–438. https://doi.org/10.1016/j.msec.2016.01.083Valliant EM, Romer F, Wang D et al (2013) Bioactivity in silica/poly(c-glutamic acid) sol-gel hybrids through calcium chelation. Acta Biomater 9:7662–7671. https://doi.org/10.1016/j.actbio.2013.04.037Shirosaki Y, Tsuru K, Hayakawa S et al (2005) In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes. Biomaterials 26:485–493. https://doi.org/10.1016/j.biomaterials.2004.02.056Romero-Gavilán F, Gomes NC, Ródenas J et al (2017) Proteome analysis of human serum proteins adsorbed onto different titanium surfaces used in dental implants. Biofouling 33:98–111. https://doi.org/10.1080/08927014.2016.1259414Hirsh SL, McKenzie DR, Nosworthy NJ et al (2013) The Vroman effect: competitive protein exchange with dynamic multilayer protein aggregates. Colloids Surfaces B Biointerfaces 103:395–404. https://doi.org/10.1016/j.colsurfb.2012.10.039Chen Z, Klein T, Murray RZ et al (2015) Osteoimmunomodulation for the development of advanced bone biomaterials. Mater Today 19:304–321. https://doi.org/10.1016/j.mattod.2015.11.004Araújo-Gomes N, Romero-Gavilán F, García-Arnáez I et al (2018) Osseointegration mechanisms: a proteomic approach. J Biol Inorg Chem 23:459–470. https://doi.org/10.1007/s00775-018-1553-9Romero-Gavilán F, Sanchez-Pérez AM, Araújo-Gomes N et al (2017) Proteomic analysis of silica hybrid sol-gel coatings: a potential tool for predicting the biocompatibility of implants in vivo. Biofouling 33:676–689. https://doi.org/10.1080/08927014.2017.1356289Araújo-Gomes N, Romero-Gavilán F, Sanchez-Pérez AM et al (2018) Characterization of serum proteins attached to distinct sol-gel hybrid surfaces. J Biomed Mater Res Part B Appl Biomater 106:1477–1485. https://doi.org/10.1002/jbm.b.33954Romero-Gavilan F, Araújo-Gomes N, Sánchez-Pérez AM et al (2017) Bioactive potential of silica coatings and its effect on the adhesion of proteins to titanium implants. Colloids Surfaces B Biointerfaces 162:316–325. https://doi.org/10.1016/j.colsurfb.2017.11.072Shiu HT, Goss B, Lutton C et al (2014) Formation of blood clot on biomaterial implants influences bone healing. Tissue Eng Part B Rev 20:697–712. https://doi.org/10.1089/ten.teb.2013.0709Wisniewski JR, Zougman A, Nagaraj N, Mann M (2009) Universal sample preparation method for proteome analysis. Nat Methods 6:3–8. https://doi.org/10.1038/NMETH.1322Dvorak MM, Riccardi D (2004) Ca2 + as an extracellular signal in bone. Cell Calcium 35:249–255. https://doi.org/10.1016/j.ceca.2003.10.014Cho NH, Seong SY (2009) Apolipoproteins inhibit the innate immunity activated by necrotic cells or bacterial endotoxin. Immunology 128:479–486. https://doi.org/10.1111/j.1365-2567.2008.03002.xMeerasa A, Huang JG, Gu FX (2013) Human serum lipoproteins influence protein deposition patterns on nanoparticle surfaces. ACS Appl Mater Interfaces 5:489–493. https://doi.org/10.1021/am302554qBaitsch D, Bock HH, Engel T et al (2011) Apolipoprotein e induces antiinflammatory phenotype in macrophages. Arterioscler Thromb Vasc Biol 31:1160–1168. https://doi.org/10.1161/ATVBAHA.111.222745Niemeier A, Schinke T, Heeren J, Amling M (2012) The role of Apolipoprotein E in bone metabolism. Bone 50:518–524. https://doi.org/10.1016/j.bone.2011.07.015Kim WS, Kim HJ, Lee ZH et al (2013) Apolipoprotein E inhibits osteoclast differentiation via regulation of c-Fos, NFATc1 and NF-κB. Exp Cell Res 319:436–446. https://doi.org/10.1016/j.yexcr.2012.12.004Emsley J, White HE, O’Hara BP et al (1994) Structure of pentameric human serum amyloid P component. Nature 367:338–345Poulsen ET, Pedersen KW, Marzeda AM, Enghild JJ (2017) Serum amyloid P component (SAP) interactome in human plasma containing physiological calcium levels. Biochemistry 56:896–902. https://doi.org/10.1021/acs.biochem.6b01027Bottazzi B, Inforzato A, Messa M et al (2016) The pentraxins PTX3 and SAP in innate immunity, regulation of inflammation and tissue remodelling. J Hepatol 64:1416–1427. https://doi.org/10.1016/j.jhep.2016.02.029Mollnes TE, Kirschfink M (2006) Strategies of therapeutic complement inhibition. Mol Immunol 43:107–121. https://doi.org/10.1016/j.molimm.2005.06.014Gessmann J, Seybold D, Peter E et al (2013) Plasma clots gelled by different amounts of calcium for stem cell delivery. Langenbeck’s Arch Surg 398:161–167. https://doi.org/10.1007/s00423-012-1015-8Scheraga HA (2004) The thrombin-fibrinogen interaction. Biophys Chem 112:117–130. https://doi.org/10.1016/j.bpc.2004.07.011Chu AJ (2010) Blood coagulation as an intrinsic pathway for proinflammation: a mini review. Inflamm Allergy Drug Targets 9:32–44. https://doi.org/10.2174/187152810791292890Suleiman L, Négrier C, Boukerche H (2013) Protein S: a multifunctional anticoagulant vitamin K-dependent protein at the crossroads of coagulation, inflammation, angiogenesis, and cancer. Crit Rev Oncol Hematol 88:637–654. https://doi.org/10.1016/j.critrevonc.2013.07.004Furie B, Furie BC (2008) Mechanisms of thrombus formation. N Engl J Med 359:938–949. https://doi.org/10.1056/NEJMra0801082Biltoft D, Gram JB, Larsen A et al (2017) Fast form alpha-2-macroglobulin—a marker for protease activation in plasma exposed to artificial surfaces. Clin Biochem 50:1203–1208. https://doi.org/10.1016/j.clinbiochem.2017.09.002Cvirn G, Gallistl S, Koestenberger M et al (2002) Alpha 2-macroglobulin enhances prothrombin activation and thrombin potential by inhibiting the anticoagulant protein C/protein S system in cord and adult plasma. Thromb Res 105:433–439. https://doi.org/10.1016/S0049-3848(02)00042-7Vogler EA, Siedlecki CA (2009) Contact activation of blood-plasma coagulation. Biomaterials 30:1857–1869. https://doi.org/10.1016/j.biomaterials.2008.12.041Leavesley DI, Kashyap AS, Croll T et al (2013) Vitronectin—master controller or micromanager? IUBMB Life 65:807–818. https://doi.org/10.1002/iub.1203Kundu AK, Putnam AJ (2006) Vitronectin and collagen I differentially regulate osteogenesis in mesenchymal stem cells. Biochem Biophys Res Commun 347:347–357. https://doi.org/10.1016/j.bbrc.2006.06.110Cacchioli A, Ravanetti F, Bagno A et al (2009) Human vitronectin-derived peptide covalently grafted onto titanium surface improves osteogenic activity: a pilot in vivo study on rabbits. Tissue Eng Part A 15:2017–2026. https://doi.org/10.1089/ten.tea.2008.054
Bioactive potential of silica coatings and its effect on the adhesion of proteins to titanium implants
There is an ever-increasing need to develop dental implants with ideal characteristics to achieve specific and desired biological response in the scope of improve the healing process post-implantation. Following that premise, enhancing and optimizing titanium implants through superficial treatments, like silica sol-gel hybrid coatings, are regarded as a route of future research in this area. These coatings change the physicochemical properties of the implant, ultimately affecting its biological characteristics. Sandblasted acid-etched titanium (SAE-Ti) and a silica hybrid sol-gel coating (35M35G30T) applied onto the Ti substrate were examined. The results of in vitro and in vivo tests and the analysis of the protein layer adsorbed to each surface were compared and discussed.
In vitro analysis with MC3T3-E1 osteoblastic cells, showed that the sol-gel coating raised the osteogenic activity potential of the implants (the expression of osteogenic markers, the alkaline phosphatase (ALP) and IL-6 mRNAs, increased). In the in vivo experiments using as model rabbit tibiae, both types of surfaces promoted osseointegration. However, the coated implants demonstrated a clear increase in the inflammatory activity in comparison with SAE-Ti. Mass spectrometry (LC–MS/MS) analysis showed differences in the composition of protein layers formed on the two tested surfaces. Large quantities of apolipoproteins were found attached predominantly to SAE-Ti. The 35M35G30T coating adsorbed a significant quantity of complement proteins, which might be related to the material intrinsic bioactivity, following an associated, natural and controlled immune response.
The correlation between the proteomic data and the in vitro and in vivo outcomes is discussed on this experimental work
- …