Comparison of the accumulation of astaxanthin in Haematococcus pluvialis and other green microalgae under N-starvation and high light conditions

Haematococcus pluvialis gave the highest astaxanthin accumulation rate (2.7 mg l−1 day−1) and total astaxanthin content ( 22.7 mg g−1 biomass). Astaxanthin accumulation in Neochloris wimmeri, Protosiphon botryoides, Scotiellopsis oocystiformis, Chorella zofingiensis and Scenedesmus vacuolatus was, respectively, 19.2, 14.3, 10.9, 6.8 and 2.7 mg astaxanthin g−1 biomass, respectively.Xunta de Galicia; PGIDT00PXI10301PR

Longitudinal Differences Observed in the Ionospheric F-Region During the Major Geomagnetic Storm of March 31, 2001

A new ionospheric sounding station using a Canadian Advanced Digital Ionosonde (CADI) was established for routine measurements by the “Universidade do Vale do Paraiba (UNIVAP)” at S˜ao Jos´e dos Campos (23.2_ S, 45.9_ W), Brazil, in August 2000. A major geomagnetic storm with gradual commencement at about 01:00 UT was observed on 31 March 2001. In this paper, we present and discuss salient features from the ionospheric sounding measurements carried out at S. J. Campos on the three consecutive UT days 30 March (quiet), 31 March (disturbed) and 1 April (recovery) 2001. During most of the storm period, the foF2 values showed negative phase, whereas during the two storm-time peaks, large F-region height variations were observed. In order to study the longitudinal differences observed in the F-region during the storm, the simultaneous ionospheric sounding measurements carried out at S. J. Campos, El Arenosillo (37.1_ N, 6.7_W), Spain, Okinawa (26.3_ N, 127.8_ E), Japan and Wakkanai (45.5_ N, 141.7_ E), Japan, during the period 30 March–1 April 2001, have been analyzed. A comparison of the observed ionospheric parameters (h0F and foF2) in the two longitudinal zones (1. Japanese and 2. Brazilian-Spanish) shows both similarities and differences associated with the geomagnetic disturbances. Some latitudinal differences are also observed in the two longitudinal zones. In addition, global ionospheric TEC maps from the worldwide network of GPS receivers are presented, showing widespread TEC changes during both the main and recovery phases of the storm. The ionospheric sounding measurements are compared with the ASPEN-TIMEGCM model runs appropriate for the storm conditions. The model results produce better agreement during the quiet period. During the disturbed period, some of the observed F-region height variations are well reproduced by the model results. The model foF2 and TEC results differ considerably during the recovery period and indicate much stronger negative phase at all the stations, particularly at the low-latitude ones

Label-Free Analysis and Sorting of Microalgae and Cyanobacteria in Microdroplets by Intrinsic Chlorophyll Fluorescence for the Identification of Fast Growing Strains.

Microalgae and cyanobacteria are promising organisms for sustainable biofuel production, but several challenges remain to make this economically viable, including identification of optimized strains with high biomass productivity. Here we report on a novel methodology for the label-free screening and sorting of cyanobacteria and microalgae in a microdroplet platform. We show for the first time that chlorophyll fluorescence can be used to measure differences in biomass between populations of picoliter microdroplets containing different species of cyanobacteria, Synechocystis PCC 6803 and Synechococcus PCC 7002, which exhibit different growth dynamics in bulk culture. The potential and robustness of this label-free screening approach is further demonstrated by the screening and sorting of cells of the green alga Chlamydomonas reinhardtii encapsulated in droplets.Author R.J.B. was supported by a Doctoral Training Grant from the Engineering and Physical Sciences Research Council (EPSRC) of the UK (Reference EP/P505445/1), and author J.J.L. was in receipt of a studentship from the Biotechnology and Biological Sciences Research Council (BBSRC) of the U.K. as part of the Cambridge BBSRC-DTP Programme (Reference BB/J014540/1). Authors S.A.-C., C.A., and A.G.S. acknowledge funding from the EC within the FP7 DEMA project, Grant Agreement No. 309086, and authors Z.Y., C.A., and A.G.S. were in receipt of funding from BBSRC sLoLa Award Reference BB/L002957/1.This is the final version of the article. It first appeared from the American Chemical Society via https://doi.org/10.1021/acs.analchem.6b0236

IRI-2001 model predictions compared with ionospheric data observed at Brazilian low latitude stations

International audienceIn this work, the F-region critical frequency (foF2) and peak height (hmF2) measured by digital ionosondes at two Brazilian low-latitude stations, namely Palmas (10.17° S, 48.20° W, dip ?10.80°) and São José dos Campos (23.20° S, 45.86° W, dip ?38.41°), are compared with the IRI-2001 model predictions. The comparison at the latter station shows quite a reasonable agreement for both parameters. The former station exhibits a better agreement for hmF2 than for foF2. In general, the model generates good results, although some improvements are still necessary to implement in order to obtain better simulations for equatorial ionospheric regions

Studies of ionospheric F-region response in the Latin American sector during the geomagnetic storm of 21–22 January 2005

In the present investigation, we have studied the response of the ionospheric F-region in the Latin American sector during the intense geomagnetic storm of 21–22 January 2005. This geomagnetic storm has been considered "anomalous" (minimum Dst reached −105 nT at 07:00 UT on 22 January) because the main storm phase occurred during the northward excursion of the B_z component of interplanetary magnetic fields (IMFs). The monthly mean <i>F</i>_10.7 solar flux for the month of January 2005 was 99.0 sfu. The F-region parameters observed by ionosondes at Ramey (RAM; 18.5° N, 67.1° W), Puerto Rico, Jicamarca (JIC; 12.0° S, 76.8° W), Peru, Manaus (MAN; 2.9° S, 60.0° W), and São José dos Campos (SJC; 23.2° S, 45.9° W), Brazil, during 21–22 January (geomagnetically disturbed) and 25 January (geomagnetically quiet) have been analyzed. Both JIC and MAN, the equatorial stations, show unusually rapid uplifting of the F-region peak heights (<i>hp</i>F2/<i>hm</i>F2) and a decrease in the <i>Nm</i>F2 coincident with the time of storm sudden commencement (SSC). The observed variations in the F-region ionospheric parameters are compared with the TIMEGCM model run for 21–22 January and the model results show both similarities and differences from the observed results. Average GPS-TEC (21, 22 and 25 January) and phase fluctuations (21, 22, 25, 26 January) observed at Belem (BELE; 1.5° S, 48.5° W), Brasilia (BRAZ; 15.9° S, 47.9° W), Presidente Prudente (UEPP; 22.3° S, 51.4° W), and Porto Alegre (POAL; 30.1° S, 51.1° W), Brazil, are also presented. These GPS stations belong to the RBMC/IBGE network of Brazil. A few hours after the onset of the storm, large enhancements in the VTEC and <i>Nm</i>F2 between about 20:00 and 24:00 UT on 21 January were observed at all the stations. However, the increase in VTEC was greatest at the near equatorial station (BELE) and enhancements in VTEC decreased with latitude. It should be pointed out that no phase fluctuations or spread-F were observed in the Latin American sector during the post-sunset pre-reversal time in the geomagnetic disturbance (21 January). The disturbance dynamo electric field possibly resulted in downward drift of the F-region plasma and inhibited the formation of spread-F

Phylogenetic relationships of cone snails endemic to Cabo Verde based on mitochondrial genomes

Background: Due to their great species and ecological diversity as well as their capacity to produce hundreds of different toxins, cone snails are of interest to evolutionary biologists, pharmacologists and amateur naturalists alike. Taxonomic identification of cone snails still relies mostly on the shape, color, and banding patterns of the shell. However, these phenotypic traits are prone to homoplasy. Therefore, the consistent use of genetic data for species delimitation and phylogenetic inference in this apparently hyperdiverse group is largely wanting. Here, we reconstruct the phylogeny of the cones endemic to Cabo Verde archipelago, a well-known radiation of the group, using mitochondrial (mt) genomes. Results: The reconstructed phylogeny grouped the analyzed species into two main clades, one including Kalloconus from West Africa sister to Trovaoconus from Cabo Verde and the other with a paraphyletic Lautoconus due to the sister group relationship of Africonus from Cabo Verde and Lautoconus ventricosus from Mediterranean Sea and neighboring Atlantic Ocean to the exclusion of Lautoconus endemic to Senegal (plus Lautoconus guanche from Mauritania, Morocco, and Canary Islands). Within Trovaoconus, up to three main lineages could be distinguished. The clade of Africonus included four main lineages (named I to IV), each further subdivided into two monophyletic groups. The reconstructed phylogeny allowed inferring the evolution of the radula in the studied lineages as well as biogeographic patterns. The number of cone species endemic to Cabo Verde was revised under the light of sequence divergence data and the inferred phylogenetic relationships. Conclusions: The sequence divergence between continental members of the genus Kalloconus and island endemics ascribed to the genus Trovaoconus is low, prompting for synonymization of the latter. The genus Lautoconus is paraphyletic. Lautoconus ventricosus is the closest living sister group of genus Africonus. Diversification of Africonus was in allopatry due to the direct development nature of their larvae and mainly triggered by eustatic sea level changes during the Miocene-Pliocene. Our study confirms the diversity of cone endemic to Cabo Verde but significantly reduces the number of valid species. Applying a sequence divergence threshold, the number of valid species within the sampled Africonus is reduced to half.Spanish Ministry of Science and Innovation [CGL2013-45211-C2-2-P, CGL2016-75255-C2-1-P, BES-2011-051469, BES-2014-069575, Doctorado Nacional-567]info:eu-repo/semantics/publishedVersio

Hemispheric asymmetries in the ionospheric response observed in the American sector during an intense geomagnetic storm

The main purpose of this investigation is to study the ionospheric F region response induced by the intense geomagnetic storm that occurred on 7-8 September 2002. The geomagnetic index Dst reached a minimum of -181 nT at 0100 UT on 8 September. In this study, we used observations from a chain of 12 GPS stations and another chain of 6 digital ionosonde stations. It should be mentioned that, soon after the sudden commencement (SC) at 1637 UT on 7 September, the TEC variations at midlatitude stations in both hemispheres showed an F region positive storm phase. However, during the recovery phase, a strong hemispheric asymmetry was observed in the ionospheric response. While a TID type soliton was observed to propagate in the Southern American sector, no TID activity was seen in the Northern American sector. Also, in the Southern Hemisphere, the TEC variations were less affected by the geomagnetic storm. The Northern Hemisphere observations showed a strong and long-lasting negative F region storm phase starting at about 1000 UT on 8 September (lasting for about 24 h). A perusal of TEC phase fluctuations and equatorial spread-F (ESF) ionospheric sounding data indicates that, on the disturbed night of 7-8 September, some stations showed the occurrence of ESF starting at about 0000 UT (2000 LT) on 8 September, whereas other stations showed that the ESF occurrence started much later, at about 0800 UT (0500 LT). This hemispheric asymmetric response of the ionospheric F region possibly indicates the presence of different mechanisms for the generation of ESF along the various latitudinal regions during the disturbed period.Facultad de Ciencias Astronómicas y Geofísica