Methodology for Complex Efficiency Evaluation of Machinery Safety Measures in a Production Organization

Even though the rules for the free circulation of machinery within the European Union (EU) market have existed for more than 30 years, accidents related to their activities have constantly been reaching significant value. When designing a machine, the design must stem from a risk assessment, where all stages of its life cycle and the ways to use it must be taken into consideration. In industrial operations with old machinery, despite fulfilling its function reliably, the safety level is below the developing requirements for safe operations. The proposed methodology to assess machinery safety conditions comes from the assumption of the proper application of risk assessment steps and their effectiveness in risk reduction mainly through implementing both effective and efficient preventive measures. The objective of the research applied in three operations was to verify the methods concerning machinery safety and its management. The created methodology, based on 19 requirements for safety, evaluates the level of current measures using a criterion of the current safety status and the total effectiveness of safety measures. Its output is the assessment of the efficiency level of the implemented safety measures of each machine as well as of the whole operation

Involvement of the mitogen-activated protein kinase SIMK in regulation of root hair tip growth

Mitogen-activated protein kinases (MAPKs) are involved in stress signaling to the actin cytoskeleton in yeast and animals. We have analyzed the function of the stress-activated alfalfa MAP kinase SIMK in root hairs. In epidermal cells, SIMK is predominantly nuclear. During root hair formation, SIMK was activated and redistributed from the nucleus into growing tips of root hairs possessing dense F-actin meshworks. Actin depolymerization by latrunculin B resulted in SIMK relocation to the nucleus. Conversely, upon actin stabilization with jasplakinolide, SIMK co-localized with thick actin cables in the cytoplasm. Importantly, latrunculin B and jasplakinolide were both found to activate SIMK in a root-derived cell culture. Loss of tip-focused SIMK and actin was induced by the MAPK kinase inhibitor UO 126 and resulted in aberrant root hairs. UO 126 inhibited targeted vesicle trafficking and polarized growth of root hairs. In contrast, overexpression of gain-of-function SIMK induced rapid tip growth of root hairs and could bypass growth inhibition by UO 126. These data indicate that SIMK plays a crucial role in root hair tip growth

Involvement of MAP kinase SIMK and actin cytoskeleton in the regulation of root hair tip growth.

International audienc

Involvement of MAP kinase SIMK and actin cytoskeleton in the regulation of root hair tip growth.

International audienc