131 research outputs found
Direct and Indirect Induction of a Compensatory Phenotype that Alleviates the Costs of an Inducible Defense
Organisms often exhibit phenotypic plasticity in multiple traits in response to impending environmental change. Multiple traits phenotypic plasticity is complex syndrome brought on by causal relations in ecological and physiological context. Larvae of the salamander Hynobius retardatus exhibit inducible phenotypic plasticity of two traits, when at risk of predation by dragonfly larvae. One induced phenotype is an adaptive defense behaviour, i.e., stasis at the bottom of water column, directly triggered by the predation risk. Another one is a compensatory phenotype, i.e., enlarged external gills, for an unavoidable cost (hypoxia) associated with the induced defense. We identified two ways by which this compensatory phenotype could be induced. The compensatory phenotype is induced in response to not only the associated hypoxic conditions resulting from the induced defense but also the most primary but indirect cause, presence of the predator
Impact of additional module training on the level of basic life support knowledge of first year students at the University of Maribor
Regulation of Kir4.1 expression in astrocytes and astrocytic tumors: a role for interleukin-1 beta
<p>Abstract</p> <p>Objective</p> <p>Decreased expression of inwardly rectifying potassium (Kir) channels in astrocytes and glioma cells may contribute to impaired K<sup>+</sup> buffering and increased propensity for seizures. Here, we evaluated the potential effect of inflammatory molecules, such as interleukin-1β (IL-1β) on Kir4.1 mRNA and protein expression.</p> <p>Methods</p> <p>We investigated Kir4.1 (Kcnj10) and IL-1β mRNA expression in the temporal cortex in a rat model of temporal lobe epilepsy 24 h and 1 week after induction of status epilepticus (SE), using real-time PCR and western blot analysis. The U373 glioblastoma cell line and human fetal astrocytes were used to study the regulation of Kir4.1 expression in response to pro-inflammatory cytokines. Expression of Kir4.1 protein was also evaluated by means of immunohistochemistry in surgical specimens of patients with astrocytic tumors (<it>n</it> = 64), comparing the expression in tumor patients with (<it>n</it> = 38) and without epilepsy (<it>n</it> = 26).</p> <p>Results</p> <p>Twenty-four hours after onset of SE, Kir4.1 mRNA and protein were significantly down-regulated in temporal cortex of epileptic rats. This decrease in expression was followed by a return to control level at 1 week after SE. The transient downregulation of Kir4.1 corresponded to the time of prominent upregulation of IL-1β mRNA. Expression of Kir4.1 mRNA and protein in glial cells in culture was downregulated after exposure to IL-1β. Evaluation of Kir4.1 in tumor specimens showed a significantly lower Kir4.1 expression in the specimens of patients with epilepsy compared to patients without epilepsy. This paralleled the increased presence of activated microglial cells, as well as the increased expression of IL-1β and the cytoplasmic translocation of high mobility group box 1 (HMGB1).</p> <p>Conclusions</p> <p>Taken together, these findings indicate that alterations in expression of Kir4.1 occurring in epilepsy-associated lesions are possibly influenced by the local inflammatory environment and in particular by the inflammatory cytokine IL-1β.</p
Evolution of scaling emergence in large-scale spatial epidemic spreading
Background: Zipf's law and Heaps' law are two representatives of the scaling
concepts, which play a significant role in the study of complexity science. The
coexistence of the Zipf's law and the Heaps' law motivates different
understandings on the dependence between these two scalings, which is still
hardly been clarified.
Methodology/Principal Findings: In this article, we observe an evolution
process of the scalings: the Zipf's law and the Heaps' law are naturally shaped
to coexist at the initial time, while the crossover comes with the emergence of
their inconsistency at the larger time before reaching a stable state, where
the Heaps' law still exists with the disappearance of strict Zipf's law. Such
findings are illustrated with a scenario of large-scale spatial epidemic
spreading, and the empirical results of pandemic disease support a universal
analysis of the relation between the two laws regardless of the biological
details of disease. Employing the United States(U.S.) domestic air
transportation and demographic data to construct a metapopulation model for
simulating the pandemic spread at the U.S. country level, we uncover that the
broad heterogeneity of the infrastructure plays a key role in the evolution of
scaling emergence.
Conclusions/Significance: The analyses of large-scale spatial epidemic
spreading help understand the temporal evolution of scalings, indicating the
coexistence of the Zipf's law and the Heaps' law depends on the collective
dynamics of epidemic processes, and the heterogeneity of epidemic spread
indicates the significance of performing targeted containment strategies at the
early time of a pandemic disease.Comment: 24pages, 7figures, accepted by PLoS ON
Heart Valve Tissue Engineering: Concepts, Approaches, Progress, and Challenges
Potential applications of tissue engineering in regenerative medicine range from structural tissues to organs with complex function. This review focuses on the engineering of heart valve tissue, a goal which involves a unique combination of biological, engineering, and technological hurdles. We emphasize basic concepts, approaches and methods, progress made, and remaining challenges. To provide a framework for understanding the enabling scientific principles, we first examine the elements and features of normal heart valve functional structure, biomechanics, development, maturation, remodeling, and response to injury. Following a discussion of the fundamental principles of tissue engineering applicable to heart valves, we examine three approaches to achieving the goal of an engineered tissue heart valve: (1) cell seeding of biodegradable synthetic scaffolds, (2) cell seeding of processed tissue scaffolds, and (3) in-vivo repopulation by circulating endogenous cells of implanted substrates without prior in-vitro cell seeding. Lastly, we analyze challenges to the field and suggest future directions for both preclinical and translational (clinical) studies that will be needed to address key regulatory issues for safety and efficacy of the application of tissue engineering and regenerative approaches to heart valves. Although modest progress has been made toward the goal of a clinically useful tissue engineered heart valve, further success and ultimate human benefit will be dependent upon advances in biodegradable polymers and other scaffolds, cellular manipulation, strategies for rebuilding the extracellular matrix, and techniques to characterize and potentially non-invasively assess the speed and quality of tissue healing and remodeling
Hyper-IgG4 disease: report and characterisation of a new disease
BACKGROUND: We highlight a chronic inflammatory disease we call 'hyper-IgG4 disease', which has many synonyms depending on the organ involved, the country of origin and the year of the report. It is characterized histologically by a lymphoplasmacytic inflammation with IgG4-positive cells and exuberant fibrosis, which leaves dense fibrosis on resolution. A typical example is idiopathic retroperitoneal fibrosis, but the initial report in 2001 was of sclerosing pancreatitis. METHODS: We report an index case with fever and severe systemic disease. We have also reviewed the histology of 11 further patients with idiopathic retroperitoneal fibrosis for evidence of IgG4-expressing plasma cells, and examined a wide range of other inflammatory conditions and fibrotic diseases as organ-specific controls. We have reviewed the published literature for disease associations with idiopathic, systemic fibrosing conditions and the synonyms: pseudotumour, myofibroblastic tumour, plasma cell granuloma, systemic fibrosis, xanthofibrogranulomatosis, and multifocal fibrosclerosis. RESULTS: Histology from all 12 patients showed, to varying degrees, fibrosis, intense inflammatory cell infiltration with lymphocytes, plasma cells, scattered neutrophils, and sometimes eosinophilic aggregates, with venulitis and obliterative arteritis. The majority of lymphocytes were T cells that expressed CD8 and CD4, with scattered B-cell-rich small lymphoid follicles. In all cases, there was a significant increase in IgG4-positive plasma cells compared with controls. In two cases, biopsies before and after steroid treatment were available, and only scattered plasma cells were seen after treatment, none of them expressing IgG4. Review of the literature shows that although pathology commonly appears confined to one organ, patients can have systemic symptoms and fever. In the active period, there is an acute phase response with a high serum concentration of IgG, and during this phase, there is a rapid clinical response to glucocorticoid steroid treatment. CONCLUSION: We believe that hyper-IgG4 disease is an important condition to recognise, as the diagnosis can be readily verified and the outcome with treatment is very good
Erratum to: 36th International Symposium on Intensive Care and Emergency Medicine
[This corrects the article DOI: 10.1186/s13054-016-1208-6.]
Beyond form and functioning: Understanding how contextual factors influence village health committees in northern India
Health committees are a common strategy to foster community participation in health. Efforts
to strengthen committees often focus on technical inputs to improve committee form
(e.g. representative membership) and functioning (e.g. meeting procedures). However,
porous and interconnected contextual spheres also mediate committee effectiveness.
Using a framework for contextual analysis, we explored the contextual features that facilitated
or hindered Village Health, Sanitation and Nutrition Committee (VHSNC) functionality
in rural north India. We conducted interviews (n = 74), focus groups (n = 18) and observation
over 1.5 years. Thematic content analysis enabled the identification and grouping of
themes, and detailed exploration of sub-themes. While the intervention succeeded in
strengthening committee form and functioning, participant accounts illuminated the different
ways in which contextual influences impinged on VHSNC efficacy. Women and marginalized
groups navigated social hierarchies that curtailed their ability to assert themselves in
the presence of men and powerful local families. These dynamics were not static and
unchanging, illustrated by pre-existing cross-caste problem solving, and the committee's
creation of opportunities for the careful violation of social norms. Resource and capacity deficits
in government services limited opportunities to build relationships between health system
actors and committee members and engendered mistrust of government institutions.
Fragmented administrative accountability left committee members bearing responsibility for
improving local health without access to stakeholders who could support or respond to their
efforts. The committee's narrow authority was at odds with widespread community needs,
and committee members struggled to involve diverse government services across the
health, sanitation, and nutrition sectors. Multiple parallel systems (political decentralization,
media and other village groups) presented opportunities to create more enabling VHSNC
contexts, although the potential to harness these opportunities was largely unmet. This
study highlights the urgent need for supportive contexts in which people can not only participate in health committees, but also access the power and resources needed to bring
about actual improvements to their health and wellbeing.IS
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