16 research outputs found
Developmental gene regulation during tomato fruit ripening and in-vitro sepal morphogenesis
BACKGROUND: Red ripe tomatoes are the result of numerous physiological changes controlled by hormonal and developmental signals, causing maturation or differentiation of various fruit tissues simultaneously. These physiological changes affect visual, textural, flavor, and aroma characteristics, making the fruit more appealing to potential consumers for seed dispersal. Developmental regulation of tomato fruit ripening has, until recently, been lacking in rigorous investigation. We previously indicated the presence of up-regulated transcription factors in ripening tomato fruit by data mining in TIGR Tomato Gene Index. In our in-vitro system, green tomato sepals cultured at 16 to 22°C turn red and swell like ripening tomato fruit while those at 28°C remain green. RESULTS: Here, we have further examined regulation of putative developmental genes possibly involved in tomato fruit ripening and development. Using molecular biological methods, we have determined the relative abundance of various transcripts of genes during in vitro sepal ripening and in tomato fruit pericarp at three stages of development. A number of transcripts show similar expression in fruits to RIN and PSY1, ripening-associated genes, and others show quite different expression. CONCLUSIONS: Our investigation has resulted in confirmation of some of our previous database mining results and has revealed differences in gene expression that may be important for tomato cultivar variation. We present new and intriguing information on genes that should now be studied in a more focused fashion
Developmental gene regulation during tomato fruit ripening and <it>in-vitro</it> sepal morphogenesis
Abstract Background Red ripe tomatoes are the result of numerous physiological changes controlled by hormonal and developmental signals, causing maturation or differentiation of various fruit tissues simultaneously. These physiological changes affect visual, textural, flavor, and aroma characteristics, making the fruit more appealing to potential consumers for seed dispersal. Developmental regulation of tomato fruit ripening has, until recently, been lacking in rigorous investigation. We previously indicated the presence of up-regulated transcription factors in ripening tomato fruit by data mining in TIGR Tomato Gene Index. In our in-vitro system, green tomato sepals cultured at 16 to 22°C turn red and swell like ripening tomato fruit while those at 28°C remain green. Results Here, we have further examined regulation of putative developmental genes possibly involved in tomato fruit ripening and development. Using molecular biological methods, we have determined the relative abundance of various transcripts of genes during in vitro sepal ripening and in tomato fruit pericarp at three stages of development. A number of transcripts show similar expression in fruits to RIN and PSY1, ripening-associated genes, and others show quite different expression. Conclusions Our investigation has resulted in confirmation of some of our previous database mining results and has revealed differences in gene expression that may be important for tomato cultivar variation. We present new and intriguing information on genes that should now be studied in a more focused fashion.</p
The RING Domain and First Zinc Finger of TRAF6 Coordinate Signaling by Interleukin-1, Lipopolysaccharide, and RANKL*
TRAF6, a crucial adaptor molecule in innate and adaptive immunity, contains
three distinct functional domains. The C-terminal TRAF domain facilitates
oligomerization and sequence-specific interaction with receptors or other
adaptor proteins. In conjunction with the dimeric E2 enzyme Ubc13-Uev1A, the
N-terminal RING domain of TRAF6 functions as an E3 ubiquitin (Ub) ligase that
facilitates its own site-specific ubiquitination through the generation of a
Lys-63-linked poly-Ub chain. This modification does not cause its proteasomal
degradation but rather serves as a scaffold to activate both the IKK and
stress kinase pathways. Connecting the N-and C-terminal regions, the four
internal zinc finger (ZF) motifs have yet to be functionally defined. In this
study, we examined the role of the ZF domains in interleukin-1,
lipopolysaccharide, and RANKL signaling by reconstitution of TRAF6-deficient
cells with point mutations or deletions of these ZF motifs. Although ZF
domains 2-4 are dispensable for activating IKK, p38, and JNK by interleukin-1
and lipopolysaccharide, the first ZF domain together with an intact RING
domain of TRAF6 is essential for activating these pathways. Furthermore, TRAF6
autoubiquitination and its interaction with Ubc13 are dependent on ZF1 and an
intact RING domain. Additionally, expression of TRAF6 lacking ZF2-4 in
TRAF6-deficient monocytes rescues RANKL-mediated osteoclast differentiation
and LPS-stimulated interleukin-6 production. These data provide evidence for
the critical role of the Ub ligase activity of TRAF6, which is coordinated via
the RING domain and ZF1 to supply the necessary elements in signaling by
cytokines dependent upon TRAF6