New Advances in iPS Cell Research Do Not Obviate the Need for Human Embryonic Stem Cells

SummaryRecently three different studies were published demonstrating that mouse fibroblast (skin) cells can be directly reprogrammed to behave like embryonic stem cells (Okita et al., 2007; Wernig et al., 2007; Maherali et al., 2007). These studies advanced a breakthrough announced last year in which a quartet of genes (Oct-3/4, Sox2, c-Myc, and Klf4) were discovered to induce pluripotency in mouse cells, albeit incompletely (Takahashi and Yamanaka, 2006). Now a second generation of these induced pluripotent stem cells (called iPS cells) has been made to do almost everything mouse embryonic stem cells can do. When mouse iPS cells were injected into mouse blastocysts, they contributed to all tissue types in the resulting adult mice, including sperm and oocytes (Okita et al., 2007; Wernig et al., 2007; Maherali et al., 2007). And one research team produced fetal mice derived entirely from iPS cells—a key criterion for embryonic stem cells (Wernig et al., 2007)

Ethical issues related to brain organoid research

This review provides a snapshot of the current ethical issues related to research with human brain organoids. The issues fall into the following main themes: research oversight; human biomaterials procurement and donor consent; translational delivery; animal research; and organoid consciousness and moral status. Each of these areas poses challenges for researchers, bioethicists, regulators, research institutions, and tissue banks. However, progress can be made if these parties build on past experiences with stem cell research, ethics, and policy, but adapted accordingly to new aspects of brain organoid research

Toward Guidelines for Research on Human Embryo Models Formed from Stem Cells.

Over the past few years, a number of research groups have reported striking progress on the generation of in vitro models from mouse and human stem cells that replicate aspects of early embryonic development. Not only do these models reproduce some key cell fate decisions but, especially in the mouse system, they also mimic the spatiotemporal arrangements of embryonic and extraembryonic tissues that are required for developmental patterning and implantation in the uterus. If such models could be developed for the early human embryo, they would have great potential benefits for understanding early human development, for biomedical science, and for reducing the use of animals and human embryos in research. However, guidelines for the ethical conduct of this line of work are at present not well defined. In this Forum article, we discuss some key aspects of this emerging area of research and provide some recommendations for its ethical oversight

A Cognitive Radio-Based Energy-Efficient System for Power Transmission Line Monitoring in Smart Grids

The research in industry and academia on smart grids is predominantly focused on the regulation of generated power and management of its consumption. Because transmission of bulk-generated power to the consumer is immensely reliant on secure and efficient transmission grids, comprising huge electrical and mechanical assets spanning a vast geographic area, there is an impending need to focus on the transmission grids as well. Despite the challenges in wireless technologies for SGs, cognitive radio networks are considered promising for provisioning of communications services to SGs. In this paper, first, we present an IEEE 802.22 wireless regional area network cognitive radio-based network model for smart monitoring of transmission lines. Then, for a prolonged lifetime of battery finite monitoring network, we formulate the spectrum resource allocation problem as an energy efficiency maximization problem, which is a nonlinear integer programming problem. To solve this problem in an easier way, we propose an energy-efficient resource-assignment scheme based on the Hungarian method. Performance analysis shows that, compared to a pure opportunistic assignment scheme with a throughput maximization objective and compared to a random scheme, the proposed scheme results in an enhanced lifetime while consuming less battery energy without compromising throughput performance

Ethical Standards for Human-to-Animal Chimera Experiments in Stem Cell Research

The purpose of this report is to offer investigators and members of SCRO and animal research committees well-grounded ethical standards for evaluating research involving the transfer of multipotent and pluripotent human stem cells and their direct derivatives into animal systems. This report is deliberately written in general terms so that its recommendations can apply to diverse institutions and international settings. Thus, investigators and reviewers should aspire to these proposed ethical standards while exercising appropriate judgment in individual situations

Rethinking organoid technology through bioengineering

In recent years considerable progress has been made in the development of faithful procedures for the differentiation of human pluripotent stem cells (hPSCs). An important step in this direction has also been the derivation of organoids. This technology generally relies on traditional three-dimensional culture techniques that exploit cell-autonomous self-organization responses of hPSCs with minimal control over the external inputs supplied to the system. The convergence of stem cell biology and bioengineering offers the possibility to provide these stimuli in a controlled fashion, resulting in the development of naturally inspired approaches to overcome major limitations of this nascent technology. Based on the current developments, we emphasize the achievements and ongoing challenges of bringing together hPSC organoid differentiation, bioengineering and ethics. This Review underlines the need for providing engineering solutions to gain control of self-organization and functionality of hPSC-derived organoids. We expect that this knowledge will guide the community to generate higher-grade hPSC-derived organoids for further applications in developmental biology, drug screening, disease modelling and personalized medicine

Differentially Expressed Potassium Channels Are Associated with Function of Human Effector Memory CD8+T cells

The voltage-gated potassium channel, Kv1.3, and the Ca2+-activated potassium channel, KCa3.1, regulate membrane potentials in T cells, thereby controlling T cell activation and cytokine production. However, little is known about the expression and function of potassium channels in human effector memory ( EM) CD8+ T cells that can be further divided into functionally distinct subsets based on the expression of the interleukin ( IL)-7 receptor alpha ( IL-7R alpha) chain. Herein, we investigated the functional expression and roles of Kv1.3 and KCa3.1 in EM CD8+ T cells that express high or low levels of the IL-7 receptor alpha chain ( IL-7R alpha(high) and IL-7R alpha(low), respectively). In contrast to the significant activity of Kv1.3 and KCa3.1 in IL-7Rahigh EM CD8+ T cells, IL-7Ralow EM CD8+ T cells showed lower expression of Kv1.3 and insignificant expression of KCa3.1. Kv1.3 was involved in the modulation of cell proliferation and IL-2 production, whereas KCa3.1 affected the motility of EM CD8+ T cells. The lower motility of IL-7Ralow EM CD8+ T cells was demonstrated using transendothelial migration and motility assays with intercellular adhesion molecule 1-and/or chemokine stromal cell-derived factor-1 alpha-coated surfaces. Consistent with the lower migration property, IL-7Ralow EM CD8+ T cells were found less frequently in human skin. Stimulating IL-7Ralow EM CD8+ T cells with IL-2 or IL-15 increased their motility and recovery of KCa3.1 activity. Our findings demonstrate that Kv1.3 and KCa3.1 are differentially involved in the functions of EM CD8+ T cells. The weak expression of potassium channels in IL-7Ralow EM CD8+ T cells can be revived by stimulation with IL-2 or IL-15, which restores the associated functions. This study suggests that IL-7Rahigh EM CD8+ T cells with functional potassium channels may serve as a reservoir for effector CD8+ T cells during peripheral inflammation.112Ysciescopu