Maturation of Moristel in Different Vineyards: Amino Acid and Aroma Composition of Mistelles and Wines with Particular Emphasis in Strecker Aldehydes

The aim of this article was to assess the influence of the harvest date on the composition of amino acids and derived aromatic compounds in grape-mistelle and wine of the Moristel variety, in different vineyards. Two vineyards were sampled in 2016 and another one in 2017. At each sampling point, grapes were collected, destemmed, crushed and divided into four aliquots. The first three were fermented, and the latter was treated with ethanol, to produce 1-week macerates containing 15% ethanol (v/v)-mistelles. Overall, 10 mistelles and 33 wines were produced. Amino acids, Strecker aldehydes and aroma compounds were analysed. Amino acid profiles are characteristic of the vineyard and level of ripeness, converging with maturation. In fermentation, major amino acids, except proline, are consumed at a relatively fixed and specific tax, while consumption of 13 amino acids is determined by the ratios of alanine, glutamic acid, serine and threonine, with γ-aminobutyric acid. After fermentation, amino acid precursors to Strecker aldehydes are maxima in unripe and overripe samples, while Strecker aldehydes are maxima in unripe wines. No direct correlations between precursor amino acids in mistelle and aromatic compounds in wine have been found. Nevertheless, must amino acid profiles could determine wine aroma composition

Determination of ppq-levels of alkylmethoxypyrazines in wine by stirbar sorptive extraction combined with multidimensional gas chromatography- mass spectrometry

Alkylmethoxypyrazines are powerful odorants in many food products. A new method for analysing 3-isopropyl- 2-methoxypyrazine, 3-s-butyl-2-methoxypyrazine and 3-isobutyl-2-methoxypyrazine has been developed and applied to wine. The analytes were extracted from 5 mL of wine using stirbar sorptive extraction followed by thermal desorption and multidimensional gas chromatography-mass spectrometry analysis in a single oven. The extraction conditions were optimized in order to obtain a high recovery of the 3-alkyl-2-methoxypyrazines (MP). The detection limits of the method in all cases were under 0.08 ng/L, well below the olfactory thresholds of these compounds in wine. The reproducibility of the method was adequate (below 10%), the linearity satisfactory and the recoveries in all cases close to 100%. The method has been applied to the analysis of 111 Spanish and French wine samples. The levels found suggest that MP have a low direct impact on the aroma properties of wines from the regions around the Pyrenean massif

A two-run heart-cut multidimensional gas chromatography method using flame ionization and mass spectrometry for automated and robust determination of nearly complete wine aroma-volatile profiles

A quantitative analytical method capable of determining the concentrations of 81 aroma-relevant wine volatiles covering nine orders of magnitude was developed and validated in this study. The method is based on stir bar sorptive extraction (SBSE) of 200 μL of wine diluted with 1.8 mL NaCl brine with pH 3.5. Volatiles thermally desorbed from the stir bars were separated in two runs in a heart-cut multidimensional gas chromatographic system and quantified using either a flame ionization detector (FID) in the first dimension (27 aroma compounds) or a mass spectrometer in the second dimension (54 aroma compounds, transferred to 22 cuts). Typical limits of compound detection lay around 0.02 mg/L by FID or ranged from 0.001 to 0.30 μg/L by mass spectrometry detector, liying below the corresponding odor thresholds in all cases. Linearity, reproducibility, and recovery were considered satisfactory for most compounds, with typical R2 values of 0.989–0.999, relative standard deviation below 10 % for 37 compounds and between 10 and 20 % for 44 compounds, and recovery rates of approximately 100 % (85–109 %) for all but acetaldehyde. An analysis of 20 wine samples completed our validation of the method, showing that a single-sample preparation procedure combined with heart-cut multidimensional two-detector gas chromatography can determine wine volatile concentrations ranging from 350 mg/L of isoamyl alcohol to 3.8 ng/L of 3-isobutyl-2-methoxypyrazine

Vall d’Hebron Risk Score II for myocardial infarction and cardiac death

Malaltia de l'artèria coronària; Factors de risc; Tomografia per emissió computeritzada de fotó únicEnfermedad arterial coronaria; Factores de riesgo; Tomografía por emisión computarizada de fotón únicoCoronary artery disease; Risk factors; Tomography, emission-computed, single-photonObjectives The aim of this study was to create a new Vall d’Hebron Risk Score-II (VH-RS-II) for non-fatal myocardial infarction (MI) and/or cardiac death (CD), excluding patients with coronary revascularisation (CR) during the follow-up. Methods We analysed 5215 consecutive patients underwent gated single photon emission CT (SPECT); 2960 patients (age 64.2±11, male 58.1%) had no previous MI and/or CR, and 2255 patients (age 63.3±11, male 81.9%) had previous MI and/or CR. During a follow-up of 4.3±2.6 years, the cardiac event (MI and CD) was evaluated. This study was reviewed and approved by the ethics committee of our institution (number form trial register, PR(AG)168.2012). To obtain the predictor model, multivariate Cox regression analysis and multivariate logistic regression analysis were used. RS-VH-II was validated with 679 patients. Results In patients without previous MI and/or CR, age (HR: 1.01; p<0.001), diabetes (HR: 2.1, p=0.001), metabolic equivalent (METs) (HR: 0.89, p=0.038), ST segment depression (HR: 1.4, p=0.011), ejection fraction (EF) (HR: 0.97, p<0.001) and summed stress score (HR: 1.2, p<0.001) were the independent predictors of CE (C-statistic: 0.8). In patients with previous MI and/or CR, age (HR: 1.06, p<0.001), male (HR: 1.9, p=0.047), smoker (HR: 1.5, p=0.047), METs (HR: 0.8, p<0.001), ST segment depression (HR: 1.4, p=0.002), EF (HR: 0.96; p<0.001) and summed difference score (HR: 1.03, p=0.06) were the independent predictors of CE (C-statistic:0.8). Conclusion The VH-RS-II obtained from different clinical exercise and gated SPECT variables allow the risk stratification for MI and CD in patients with or without previous MI and/or CR in due form

Estudio del sistema de marcapasos auriculares en el corazón del perro

Inclou referències bibliogràfique

Bin-CE: A comprehensive web application to decide upon the best set of outcomes to be combined in a binary composite endpoint

The estimation of the Sample Size Requirement (SSR) when using a binary composite endpoint (i.e. two or more outcomes combined in a unique primary endpoint) is not trivial. Besides information about the rate of events for each outcome, information about the strength of association between the outcomes is crucial, since it can determine an increase or decrease of the SSR. Specifically, the greater the strength of association between outcomes the higher the SSR. We present Bin-CE, a free tool to assist clinicians for computing the SSR for binary composite endpoints. In a first step, the user enters a set of candidate outcomes, the assumed rate of events for each outcome and the assumed effect of therapy on each outcome. Since the strength of the association between outcomes is usually unknown, a semi-parametric approach linking the a priori clinical knowledge of the potential degree of association between outcomes with the exact values of these parameters was programmed with Bin-CE. Bin-CE works with a recursive algorithm to choose the best combination of outcomes that minimizes the SSR. In addition, Bin-CE computes the sample size using different algorithms and shows different figures plotting the magnitude of the sample size reduction, and the effect of different combinations of outcomes on the rate of the primary endpoint. Finally, Bin-CE is programmed to perform sensitivity analyses. This manuscript presents the mathematic bases and introduces the reader to the use of Bin-CE using a real example

Description of fourth instar larva and pupa of Atrichopogon delpontei Cavalieri and Chiossone (Diptera: Ceratopogonidae) from Brazilian Amazonia

The fourth instar larva and pupa of Atrichopogon delpontei Cavalieri and Chiossone are described for the first time. The immatures were collected from stream margins in the northern Brazilian states Rondônia and Piauí, and subsequently reared to adults. Larvae and pupae are illustrated and photomicrographed. Details on the rearing process and feeding behavior in laboratory, bionomics and notes on habitats are also provided.Instituto de Limnología "Dr. Raul A. Ringuelet"Centro de Estudios Parasitológicos y de Vectore

EVALUATION AND GROUPING OF SUGARCANE GENOTYPES IN AGREEMENT WITH THEIR PHYSIOLOGIC CHARACTERISTICSOTYPES

AbstractThe objective of this work was to evaluate physiological characteristics of sugar cane genotypes, as well as characterize them in groups according to their similarity, checking the ability of ecological adaptability of these genotypes. The work was performed in field conditions, being assessed ten sugarcane genotypes (RB855113, RB835486, RB867515, SP80-1816, RB72454, RB925345, RB855156, RB937570, RB947520 and RB925211) in a randomized block design with three repetitions. It were evaluated the stomatal gas flow rate (U - μ mol s-1), the concentration of under-stomatal CO2 (Ci - μmol mol-1), the photosynthetic rate (A - μmol m-2 s-1), the CO2 consumed (Δ C - μmol mol-1), the stomatal conductance (Gs - mol m-1 s-1), the temperature gradient between leaf and air (Δ T), and the transpiration rate  (E - mol H2O m-2 s-1), being also calculated the water use efficiency (WUE - mol CO2 mol H2O-1) from the values of the amount of CO2 fixed by photosynthesis and amount of water transpirated. Both univariate and multivariate data analysis were made. The genotype SP80-1816 showed better water use efficiency, combined with low stomatal conductance and transpiratory rate. The cultivar RB855113 stood out by having high photosynthetic rate, and high consumption of CO2. The cultivar RB867515, in addition to showing high water use efficiency, also showed high photosynthetic rate. With respect to the multivariate analysis, the biotypes RB925345, RB925211, RB855156 and RB855113 are situated in different groups when compared to the others as to the physiological characteristics with respect to other genotypes with isolation in separate groups.Keywords: Saccharum spp. photosynthesis. Transpiration. Cultivars

Organizational life cycle assessment: suitability for higher education institutions with environmental management systems

[EN] Purpose The purpose of this study is to analyze the suitability of organizational life cycle assessments (O-LCAs) for higher education institutions (HEIs) with special attention to the benefits and particularities of those adopting environmental management systems (EMSs) verified according to Environmental Management and Audit Scheme (EMAS). Methods A thorough analysis following ISO/TS 14072 and UNEP Guidance was carried out using the Universitat Politècnica de València (UPV) EMS verified by the EMAS for guiding principles to develop the methodological proposal. The self-sufficiency of UPV EMS for developing an O-LCA was tested at the university pilot unit. The four steps of the O-LCA were applied to the pilot. Results and discussion A reporting organization, the organization to be studied (boundaries and scope), was defined in consideration of the environmental units (EU) of the EMS. Operational control was selected as a consolidation method. Reporting flows and system boundaries are also discussed. A three-scope scheme of the GHG protocol is introduced and combined with the ISO 14072 boundary definition to support better alignment with the HEI structure. For the life cycle inventory analysis, a mechanism for identifying activities and processes as well as their material and energy flows is proposed in consideration of the particularities of HEIs. A procedure for the prioritization of data collection efforts and cutoffs was developed. The procedure integrates current EMAS actions based on the significance of environmental aspects combined with the influence of reporting organizations under their control. Impact categories focus on midpoint indicators along with an additional inventory level indicator as part of the life cycle impact assessment (LCIA). Unfortunately, due to a lack of quality data available, LCIA can only be assessed in part with little interest in outcomes. Partial results are presented. Conclusions An EMS verified by EMAS is proven to be useful in the assessment of O-LCA for HEIs. However, EMAS requirements do not ensure the availability of all data needed to develop an O-LCA. An accounting system should complement a lack of data if it is properly structured. Considerable efforts are required to obtain an accurate result. EMS and the accounting system may be able to provide information that supports an O-LCA approach based on a coherent prioritization of data collection efforts and cutoff procedures along with a set of justified impact category indicators. Overall, organization managers must be in favor of such an assessment to meet the requirements of successful implementation.Lo-Iacono-Ferreira, VG.; Torregrosa López, JI.; Capuz-Rizo, SF. (2017). Organizational life cycle assessment: suitability for higher education institutions with environmental management systems. 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