Application of liquid chromatography in substitution of the radioimmunoassay technique in order to reduce residues generated in health services in research laboratory

Designing a Health Care Service Waste Management Plan, according to the RDC 306 rules, is a responsibility of all those who produce such waste. Since radioimmunoassay (RIA) is one of the most employed techniques, we studied the impact of replacing this technique by liquid chromatography (HPLC) with regard to the reduction of the radioactive residues routinely produced by the UNIFESP steroid laboratory. The residues produced by the determination of serum cortisol and 17 α-hydroxyprogesterone were classified, and those belonging to groups B and C were evaluated. We observed that, when RIA is used, chemical residues (group B) and radioactive waste (group C) are produced, whereas HPLC generates only chemical residues. Adequation of these techniques showed to be advantageous, by significantly reducing the time of analysis and mainly by eliminating and/or reducing the generation of radioactive waste, encouraging its application to other methodologies, as well as its adoption by other research units.A elaboração do Plano de Gerenciamento dos Resíduos dos Serviços de Saúde, normatizado na RDC nº 306/2004, é de responsabilidade de todos os geradores. Sendo o radioimunoensaio (RIE) uma das técnicas mais empregadas, estudou-se o impacto da substituição dessa técnica por cromatografia líquida (HPLC), no aspecto da redução na geração de resíduos radioativos na rotina do Laboratório de Esteróides da Universidade Federal de São Paulo (UNIFESP). Os resíduos gerados nas determinações de cortisol e 17α-hidroxiprogesterona séricos foram classificados, e aqueles pertencentes aos grupos B e C foram avaliados. Observou-se que no emprego de RIE há geração de resíduos químicos (grupo B) e rejeitos radioativos (grupo C), enquanto no HPLC gerou-se apenas resíduos químicos. A adequação dessas técnicas teve vantagem de redução significativa no tempo de análise e, sobretudo, a eliminação e/ou a diminuição na geração de rejeitos radioativos, estimulando a sua aplicação para outras metodologias, bem como a implantação em outras unidades de pesquisas.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo (UNIFESP) Departamento de Medicina Laboratório de EsteróidesUNIFESP, Depto. de Medicina Laboratório de EsteróidesSciEL

Pressure-driven instability in auroral images to create auroral patches

第3回極域科学シンポジウム/第36回極域宙空圏シンポジウム 11月27日（火） 国立極地研究所 2階大会議

Multicomponent Bright Solitons in F = 2 Spinor Bose-Einstein Condensates

We study soliton solutions for the Gross--Pitaevskii equation of the spinor Bose--Einstein condensates with hyperfine spin F=2 in one-dimension. Analyses are made in two ways: by assuming single-mode amplitudes and by generalizing Hirota's direct method for multi-components. We obtain one-solitons of single-peak type in the ferromagnetic, polar and cyclic states, respectively. Moreover, twin-peak type solitons both in the ferromagnetic and the polar state are found.Comment: 15 pages, 8 figure

Electrically tunable spin injector free from the impedance mismatch problem

Injection of spin currents into solids is crucial for exploring spin physics and spintronics. There has been significant progress in recent years in spin injection into high-resistivity materials, for example, semiconductors and organic materials, which uses tunnel barriers to circumvent the impedance mismatch problem; the impedance mismatch between ferromagnetic metals and high-resistivity materials drastically limits the spin-injection efficiency. However, because of this problem, there is no route for spin injection into these materials through low-resistivity interfaces, that is, Ohmic contacts, even though this promises an easy and versatile pathway for spin injection without the need for growing high-quality tunnel barriers. Here we show experimental evidence that spin pumping enables spin injection free from this condition; room-temperature spin injection into GaAs from Ni81Fe19 through an Ohmic contact is demonstrated through dynamical spin exchange. Furthermore, we demonstrate that this exchange can be controlled electrically by applying a bias voltage across a Ni81Fe19/GaAs interface, enabling electric tuning of the spin-pumping efficiency

Estruturas organizacionais de empresas digitais: estudo de caso uma empresa ágil no ramo de seguros: Digital organization´s structure: a case of insurance agile company

As empresas digitais vêm transformando a percepção dos negócios atuais desde sua essência, até a forma de sua organização, cargos e atividades. Hoje com a tecnologia e informação como sendo protagonista de setores tradicionais, a busca pelo atendimento as necessidades dos clientes se tornam cada dia mais importante e as empresas procuram cada vez mais se tornarem ágeis e eficientes. As “insurtechs” são empresas digitais na área de seguros e vem transformando, em muitos sentidos, o ambiente tradicional de seguros em que as empresas estavam acostumadas e estagnadas. O presente trabalho vem colaborar com uma visão de organização ágil dentro do contexto das empresas de seguro, trazendo um estudo de caso da estrutura organizacional que se utiliza do ágil com foco no cliente e que busca esse diferencial para concorrer com as empresas tradicionais em um mercado ainda bastante tradicional

Effect of the FACs distribution on the middle and low latitude ionospheric current patterns deduced by a 2-D ionospheric potential solver (GEMSIS-POT)

第2回極域科学シンポジウム/第35回極域宙空圏シンポジウム 11月15日（火） 国立極地研究所 2階大会議室前フロ

Correlation between pulsating auroras and chorus waves observed at Athabasca (L=4.4), Canada

第3回極域科学シンポジウム/第36回極域宙空圏シンポジウム 11月26日（月）、27日（火） 国立極地研究所 2階ラウン

Longitudinal development of a substorm brightening arc

We present simultaneous THEMIS-ground observations of longitudinal (eastward) extension of a substorm initial-brightening arc at Gillam (magnetic latitude: 65.6°) at 08:13 UT on 10 January 2008. The speed of the eastward arc extension was ~2.7 km/s. The extension took place very close to the footprints of the longitudinally separated THEMIS E and D satellites at ~12 <I>R<sub>E</sub></I>. The THEMIS satellites observed field dipolarization, weak earthward flow, and pressure increase, which propagated eastward from E to D at a speed of ~50 km/s. The THEMIS A satellite, located at 1.6 <I>R<sub>E</sub></I> earthward of THEMIS E, observed fluctuating magnetic field during and after the dipolarization. The THEMIS E/D observations suggest that the longitudinal extension of the brightening arc at substorm onset is caused by earthward flow braking processes which produce field dipolarization and pressure increase propagating in longitude in the near-earth plasma sheet

High intake of phytoestrogens and precocious thelarche: case report with a possible correlation

Precocious thelarche is the breast development before 8 years of age with two peaks of incidence during the first two years of life and after 6 years of age. A 4.75-year-old girl presented with thelarche associated to an excessive intake of phytoestrogens (phye). Tanner development B2P1-2, hormonal levels and pelvic US were compatible with peripheral precocious puberty. During follow-up, a daily intake of soy-based foods was observed (> 40 mg phye/day). Soy is rich in phytoestrogens, mainly genistein and daidzein. Although phye are less strong than estradiol, its concentration could be from 13,000 to 22,000 times stronger in children fed only by soy-based formulas. Parents were advised and soy intake was reduced to once a week. Progression of pubertal development ceased at B2-3P1. The patient, now 8.66 years old, keeps growing with similar bone and chronological ages. Some questions related to industrial food security, mainly the soy-based food, remain without precise answer. Although it is well known the entity of non-progressive precocious puberty and premature thelarche, pubertal development in this case was strongly related to excessive daily intake of soy and other phye-rich food that could trigger puberty as endocrine disruptor.Telarca precoce, desenvolvimento mamário antes dos 8 anos de idade, apresenta dois picos de incidência, nos dois primeiros anos de vida e após os 6 anos de idade. Uma menina de 4,75 anos apresentou-se com telarca associada à ingestão excessiva de fitoestrógenos. O desenvolvimento puberal M2P1-2, os níveis hormonais e o US pélvico eram compatíveis com puberdade precoce periférica. Durante o seguimento, observou-se ingestão diária de alimentos baseados em soja (> 40 mg fitoestrógenos/dia). Soja contém fitoestrógenos, principalmente genisteína e daidzeína. Embora menos potentes do que o estradiol, sua concentração pode ser 13.000 a 22.000 vezes maior em crianças alimentadas somente com fórmulas baseadas em soja. Os pais foram aconselhados a reduzir para uma vez na semana o seu fornecimento na dieta. O desenvolvimento puberal cessou em M2-3P1 e a paciente, com 8,66 anos, continua a desenvolver-se com idades óssea e cronológica equivalentes. Questões relacionadas à segurança alimentar, principalmente de alimentos baseados em soja, permanecem sem respostas precisas. Embora sejam conhecidas as etiologias da puberdade precoce não progressiva e da telarca prematura, neste caso esteve fortemente relacionada à ingestão excessiva de soja e de outros alimentos ricos em fitoestrógenos que poderiam ter desencadeado a telarca atuando como desreguladores endócrinos.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de MedicinaUNIFESP, EPM, Depto. de MedicinaSciEL