Interconnectivity of habitats in soil:combining X-ray micro tomography and thin sectioning to reveal fungal-soil structure interactions

The extreme heterogeneity and interconnectivity of the 3-dimensional pore space within soil makes it a unique habitat for the diverse microbial population and has a pivotal role in microbial interactions. Manipulation and quantification of the 3-dimensional pore space and the spatial distribution of micro-organisms is therefore essential if we are to fully understand microbial interactions. Here we pack soil microcosms at different bulk-densities to manipulate soil structure and use x-ray micro tomography and soil thin sections to analyse the effect on the connectivity of the pore volume and on fungal exploration

The evolution of layered, basic plutons - evidence from small-scale structures

Many basic and ultrabasic plutons are commonly lithologically, texturally and compositionally layered on several scales. Associated with and defined by, the layering are a variety of small-scale structures, considered analogous to structures found in sedimentary rocks, and capable of interpretation using similar techniques. Observations on these small-scale structures are vised to infer the "depositional" and "diagenetic" processes that operated during the solidification of a number of' plutons. Erosion structures and structures associated with lithic fragments indicate that physical redistribution of solids, crystals and rock fragments, was an important process in the evolution of these bodies and that plagioclase was deposited at the floor even where it was less dense than the contemporary magma. From their relationships with lithic fragments normally graded layers are demonstrated to be the solidified remnants of crystal-laden density currents flowing across the transient floor of the magma chamber and are inferred to be the best approximation to truly chronostratigraphic horizons in layered plutons identified to date. The proposition that crystal- and rock-laden density currents can transport material vertically through the magma body is investigated experimentally by examining the nature of such flows in a small tank, Experiments and calculations indicate that such density currents straddle the range of conditions from laminar to fully turbulent. Laminar flows do not mix with the contemporary magma and will transport crystals to the transient chamber floor. It is suggested that such a process may have given rise to lensoid mafic layers of limited areal extent in the Kiglapait intrusion. Structures inferred to have formed during the "diagenetic" stage of the formation of the layered rocks include deformation structures, layers and replacement bodies. Examples of the latter two sets of structures are shown to have textures identical to those in rocks interpreted as cumulates and it is concluded that those textures alone are not sufficient basis on which to infer crystal growth from the contemporary magma. Many of the structures testify to the former presence of mobile pore liquids and the contemporaneity of pore liquids of different compositions. Movement of pore liquid is considered to have been driven by density differences due to variations in pore magma composition and thus the structures can be considered as evidence for interstitial compositional convection. Under certain circumstances pore liquids may be expelled from the crystal mush and mix with the contemporary magma. The chemical consequences of such mixing events are discussed and it is proposed that chromitite layers in the Eastern Layered Series of the Rum intrusion record the operation of the process. Several features of the Rum intrusion suggest that the magma chamber was thermally and compositionally zoned at times during its active history and this leads to the formulation of a new scheme for the formation of cyclic stratigraphy in the Rum layered intrusion, based on the progradation and regression of a liquid/liquid interface and two environments of accumulation

A 520 year record of summer sunshine for the eastern European Alps based on stable carbon isotopes in larch tree rings

A 520-year stable carbon isotope chronology from tree ring cellulose in high altitude larch trees (Larix decidua Mill.), from the eastern European Alps, correlates more strongly with summer temperature than with summer sunshine hours. However, when instrumental records of temperature and sunshine diverge after AD 1980, the tree ring time series does not follow warming summer temperatures but more closely tracks summer sunshine trends. It is concluded that sunshine is the dominant control on carbon isotope fractionation in these trees, via the influence of photosynthetic rate on the internal partial pressure of CO2, and that high summer (July-August) sunshine hours is a suitable target for climate reconstruction. We thus present the first reconstruction of summer sunshine for the eastern Alps and compare it with the regional temperature evolution

Interactions between soil structure and fungi

The spatial organisation of soils is crucially important in affecting belowground function, and the associated delivery of ecosystem services. Fungi constitute an important part of the soil biomass. As well as playing key roles in nutrient cycling and biotic interactions, they are also intimately involved in soil structural dynamics. Fungi mediate the formation of soil structure at a variety of spatial scales via charge, adhesive and enmeshment mechanisms. They also produce large quantities of hydrophobic compounds that affect water infiltration properties of soils. Fungi can also destroy soil structure via decomposition of organic matter that affects soil aggregation. In turn, soil structure affects fungi. The filamentous growth-form of fungi is a very efficient spacefilling structure well adapted for life in a spatially heterogeneous environment such as soil, but the labyrinthine pore network ultimately regulates how fungal mycelia grow through and function within the soil. The distribution of water within soils plays a crucial role in governing fungal development and activity, as does the spatial distribution of nutrient resources. This article reviews the continual interplay that occurs between soil structure and fungi, and discusses how self-organisation mechanisms may operate in the soil system

Effects of carbon dioxide on the searching behaviour of the root-feeding clover weevil <i>Sitona lepidus</i> (Coleoptera: Curculionidae)

The respiratory emission of CO2 from roots is frequently proposed as an attractant that allows soil-dwelling insects to locate host plant roots, but this role has recently become less certain. CO2 is emitted from many sources other than roots, so does not necessarily indicate the presence of host plants, and because of the high density of roots in the upper soil layers, spatial gradients may not always be perceptible by soil-dwelling insects. The role of CO2 in host location was investigated using the clover root weevil Sitona lepidus Gyllenhall and its host plant white clover (Trifolium repens L.) as a model system. Rhizochamber experiments showed that CO2 concentrations were approximately 1000 ppm around the roots of white clover, but significantly decreased with increasing distance from roots. In behavioural experiments, no evidence was found for any attraction by S. lepidus larvae to point emissions of CO2, regardless of emission rates. Fewer than 15% of larvae were attracted to point emissions of CO2, compared with a control response of 17%. However, fractal analysis of movement paths in constant CO2 concentrations demonstrated that searching by S. lepidus larvae significantly intensified when they experienced CO2 concentrations similar to those found around the roots of white clover (i.e. 1000 ppm). It is suggested that respiratory emissions of CO2 may act as a ‘search trigger’ for S. lepidus, whereby it induces larvae to search a smaller area more intensively, in order to detect location cues that are more specific to their host plant.<br/

Mapping the e-Learning Assessment Domain: Concept Maps for Orientation and Navigation

Concept or Topic Maps have long been used as a method of categorizing and organizing information about a domain. Building them can help people conceptualize an area and spot trends or gaps, and as a presentation method they quickly provide an overview and general impression of a space. We are currently constructing a Reference Model of the Assessment Domain that takes the form of a highly interlinked dynamic website. This represents the assessment domain via the software, projects, standards and use cases of which it is composed. In this paper we present our efforts to create complimentary concept maps of the assessment domain, not as an overview, but for navigation and orientation within the domain. These concept maps, which model resources and activities independently, have been corroborated with practitioners in the e-learning community

Rapid Detection of the Antibiotic Sulfamethazine in Pig Body Fluids by Paper Spray Mass Spectrometry

We report herein a practical method for nonlethal detection of the antibiotic sulfamethazine in pig body fluids via the combination of simple extraction and paper spray mass spectrometry (PS-MS). This method requires minimal sample preparation while still providing high sensitivities and accuracies in complex matrices including pig whole blood (LOD = 7.9 μg/L; recovery = 95.4–103.7%), pig serum (LOD = 11.5 μg/L; recovery = 103.2–106.2%), and synthetic urine (LOD = 11.2 μg/L; recovery = 99.1–103.2%). Given a known correlation between the level of sulfamethazine in body fluids and edible tissues, this method shows great promise as a practical and nonlethal solution for rapid testing of the drug, which can substantially aid managerial decision in the livestock industry

CD40 ligand induced cytotoxicity in carcinoma cells is enhanced by inhibition of metalloproteinase cleavage and delivery via a conditionally-replicating adenovirus

Background CD40 and its ligand (CD40L) play a critical role in co-ordinating immune responses. CD40 is also expressed in lymphoid malignancies and a number of carcinomas. In carcinoma cells the physiological outcome of CD40 ligation depends on the level of receptor engagement with low levels promoting cell survival and high levels inducing cell death. The most profound induction of cell death in carcinoma cells is induced by membrane-bound rather than recombinant soluble CD40L, but like other TNF family ligands, it is cleaved from the membrane by matrix metalloproteinases. Results We have generated a replication-deficient adenovirus expressing a mutant CD40L that is resistant to metalloproteinase cleavage such that ligand expression is retained at the cell membrane. Here we show that the mutated, cleavage-resistant form of CD40L is a more potent inducer of apoptosis than wild-type ligand in CD40-positive carcinoma cell lines. Since transgene expression via replication-deficient adenovirus vectors in vivo is low, we have also engineered a conditionally replicating E1A-CR2 deleted adenovirus to express mutant CD40L, resulting in significant amplification of ligand expression and consequent enhancement of its therapeutic effect. Conclusions Combined with numerous studies demonstrating its immunotherapeutic potential, these data provide a strong rationale for the exploitation of the CD40-CD40L pathway for the treatment of solid tumours

The heterogeneous soil environment:are there preferential pathways for fungal spread?

Most studies with soil-borne pathogenic fungi have been done with little explicit characterisation of soil structure within which fungi spread and biotic interactions occur. Soil, however, constitutes a framework of surfaces formed by old root channels, cracks or biopores in combination with aggregates. Using epidemiological and soil biological techniques in controlled environments we investigated the effect of soil heterogeneity on fungal growth dynamics. We show that cracks and larger pores can act either as preferential pathways or barriers for the spread of fungal plant pathogens through soil. Understanding the effect of soil structure on pathogen and antagonist dynamics is therefore critical for our understanding of epidemics and the development of control strategies in a heterogeneous environment