Análise antropométrica e dietética de surfistas amadores

O Surf é um esporte popular no Brasil, porém dados referentes à antropometria e dietéticos nos surfistas brasileiros ainda são limitados. Sendo assim, o objetivo da presente pesquisa foi verificar as características antropométricas e nutricionais de surfistas amadores brasileiros. Para tanto foram analisados seis surfistas do sexo masculino com idade média de 27,6 ± 2,7 anos, massa corporal de 75 ± 7,8 kg e estatura de 179,3 ± 5,4 cm. O percentual de gordura foi determinado por meio da bioimpedância elétrica e os valores encontrados foram de 15,6 ± 3,6 %. Para averiguar o consumo alimentar dos atletas foi utilizado o questionário Semi-quantitativo de frequência alimentar. Em relação ao grupo óleos 39,1%, cereais e leguminosas 41,5%, produtos diet e light 70,6% raramente ou nunca os mesmos eram consumidos p˂0,05. Em conclusão, os sufistas do presente estudo, se apresentaram mais altos, pesados e com %G superior aos registrados pela literatura, e mostraram uma inadequação alimentar, não coerente e balanceada que contemplem as necessidades diárias de nutrientes, para sua prática esportiva. ABSTRACT Anthropometric and dietetic analysis in amateur surfersSurfing is a popular sport in Brazil, however, data on anthropometry and dietetics in Brazilian surfers are still limited. Therefore, the objective of the present research was to verify the anthropometric and nutritional characteristics of Brazilian amateur surfers. For that, six surfers were analyzed with the mean age of 27.6 ± 2.7, body mass of 75 ± 7.8 kg and 179.3 ± 5.4 cm height. The percentage of fat was determined by electrical bioimpedance and the found values were 15.6 ± 3.6 %. The semi-quantitative food frequency questionnaire was used to determine the food intake of the athletes. In relation to the group oils 39.1%, cereals and legumes 41.5%, diet and light products 70.6% rarely or never they were consumed p˂0.05. In conclusion, the surfers of this study, they showed higher, heavier and G% higher than recorded in the literature, and showed an inadequate diet, not consistent and balanced that address the daily nutrient requirements for your sport

Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56

Bacterial AB toxins are secreted key virulence factors that are internalized by target cells through receptor-mediated endocytosis, translocating their enzymatic domain to the cytosol from endosomes (short-trip) or the endoplasmic reticulum (long-trip). To accomplish this, bacterial AB toxins evolved a multidomain structure organized into either a single polypeptide chain or non-covalently associated polypeptide chains. The prototypical short-trip single-chain toxin is characterized by a receptor-binding domain that confers cellular specificity and a translocation domain responsible for pore formation whereby the catalytic domain translocates to the cytosol in an endosomal acidification-dependent way. In this work, the determination of the three-dimensional structure of AIP56 shows that, instead of a two-domain organization suggested by previous studies, AIP56 has three-domains: a non-LEE encoded effector C (NleC)-like catalytic domain associated with a small middle domain that contains the linker-peptide, followed by the receptor-binding domain. In contrast to prototypical single-chain AB toxins, AIP56 does not comprise a typical structurally complex translocation domain; instead, the elements involved in translocation are scattered across its domains. Thus, the catalytic domain contains a helical hairpin that serves as a molecular switch for triggering the conformational changes necessary for membrane insertion only upon endosomal acidification, whereas the middle and receptor-binding domains are required for pore formation. © 2023, The Author(s).This work was supported by National funds through FCT under the project UIDB/04293/2020 and by FEDER funds through Programa Operacional Factores de Competitividade – COMPETE and by national funds through FCT – Fundação para a Ciência e a Tecnologia under the project PTDC/BIA-MIC/29910/2017 to N.M.S.S. A.d.V. was funded by Portuguese national funds through the FCT and, when eligible, by COMPETE 2020 FEDER funds, under the Scientific Employment Stimulus–Individual Call 2021.02251.CEECIND/CP1663/CT0016. We acknowledge access to the HTX crystallization facility (Proposal ID: BIOSTRUCTX_8167) and SOLEIL, ESRF and ALBA synchrotrons for provision of measurement time and thank their staff for help with data collection. The authors acknowledge the support of i3S Scientific Platforms (https://www.i3s.up.pt/scientific-platforms.php) Advanced Light Microscopy, member of the national infrastructure PPBI-Portuguese Platform of BioImaging (supported by POCI-01-0145-FEDER-022122), Animal Facility, Biochemical and Biophysical Technologies and X-ray Crystallography. A special thanks to Dr. Marc Graille and Dr. João Morais Cabral for constructive discussions in structural biology and Dr. Dimitri Panagiotis Papatheodorou for providing plasmid p327

Conduta nutricional de bailarinos adolescentes de ambos os sexos

A balanced diet is a major factor for dancers because of their contribution to improving the body composition and physical fitness of this audience. The objective of the research was to evaluate the nutritional behavior of macro- and micronutrients in the diet of classic adolescent dancers. Nine male dancers of 16.4 ± 1.5 years old and female 14.5 ± 0.9 years old were evaluated. The fat percentage showed values of 8.8 ± 1.3% and 8.7 ± 2.2% for boys and girls in this order. The food consumption of the dancers was recorded in the semi-quantitative food frequency questionnaire. Boys rarely consumed milks and derivatives 28.8%, vegetables and fruits 70%, beverages 35.7% and products diet or light 100% where p˂0.05. Girls consumed snacks and canned once a week 66.7% and rarely drinks 84.6% and diet light products 92.9% where p˂0.05. Macronutrient intake was determined through a three-day dietary recall. The percentage of carbohydrate in the diet of the dancers was 57.8 ± 16.1%, of the dancers 59.7 ± 5.5%. For the protein the value for the dancers was 18 ± 6.2% and 17 ± 2.9 for the dancers. Considering lipids, the ingested numbers were 19.6 ± 7.6% and 21.7 ± 4.7% in this order for boys and girls where, p≥0.05. In conclusion, the dancers of the present study showed a feeding inadequacy, not consistent with the daily nutrient requirements for their dance modality.Uma alimentação balanceada constitui um fator primordial para os bailarinos, devido a sua contribuição para melhorar a composição corporal e aptidão física deste público. O objetivo da pesquisa foi avaliar a conduta nutricional de macro- e micronutrientes na dieta de bailarinos clássicos adolescentes. Foram avaliados nove bailarinos, do sexo masculino com 16,4 ± 1,5 anos e feminino 14,5 ± 0,9 anos. O percentual de gordura mostrou valores de 8,8 ± 1,3% e 8,7 ± 2,2% para os meninos e meninas nesta ordem. O consumo alimentar dos bailarinos, foi registrado no questionário semi-quantitativo de frequência alimentar. Os meninos raramente consumiam leites e derivados 28,8%, hortaliças e frutas 70%, bebidas 35,7% e produtos diet ou light 100% onde p˂0,05. As meninas consumiam petiscos e enlatados 1 vez por semana 66,7% e raramente bebidas 84,6% e produtos diet light 92,9% onde p˂0,05. A ingesta de macronutrientes foi determinada por intermédio de um recordatório alimentar de três dias. O percentual de carboidrato ingerido na dieta dos bailarinos foi de 57,8 ± 16,1% e das bailarinas de 59,7 ± 5,5%. Para as proteínas o valor para os dançarinos foi de 18 ± 6,2% e de 17 ± 2,9 para as dançarinas. Sobre os lipídios os numerários ingeridos foram de 19,6 ± 7,6% e 21,7 ± 4,7% nesta ordem para meninos e meninas onde, p≥0,05. Em conclusão, os bailarinos do presente estudo, mostraram uma inadequação alimentar, não coerentes com as necessidades diárias de nutrientes, para sua modalidade de dança. ABSTRACT Nutritional conduct of adolescent dancers of both sexA balanced diet is a major factor for dancers because of their contribution to improving the body composition and physical fitness of this audience. The objective of the research was to evaluate the nutritional behavior of macro- and micronutrients in the diet of classic adolescent dancers. Nine male dancers of 16.4 ± 1.5 years old and female 14.5 ± 0.9 years old were evaluated. The fat percentage showed values of 8.8 ± 1.3% and 8.7 ± 2.2% for boys and girls in this order. The food consumption of the dancers was recorded in the semi-quantitative food frequency questionnaire. Boys rarely consumed milks and derivatives 28.8%, vegetables and fruits 70%, beverages 35.7% and products diet or light 100% where p˂0.05. Girls consumed snacks and canned once a week 66.7% and rarely drinks 84.6% and diet light products 92.9% where p˂0.05. Macronutrient intake was determined through a three-day dietary recall. The percentage of carbohydrate in the diet of the dancers was 57.8 ± 16.1%, of the dancers 59.7 ± 5.5%. For the protein the value for the dancers was 18 ± 6.2% and 17 ± 2.9 for the dancers. Considering lipids, the ingested numbers were 19.6 ± 7.6% and 21.7 ± 4.7% in this order for boys and girls where, p≥0.05. In conclusion, the dancers of the present study showed a feeding inadequacy, not consistent with the daily nutrient requirements for their dance modality

Rat Adipose Tissue-Derived Stem Cells Transplantation Attenuates Cardiac Dysfunction Post Infarction and Biopolymers Enhance Cell Retention

Background: Cardiac cell transplantation is compromised by low cell retention and poor graft viability. Here, the effects of co-injecting adipose tissue-derived stem cells (ASCs) with biopolymers on cell cardiac retention, ventricular morphometry and performance were evaluated in a rat model of myocardial infarction (MI). Methodology/Principal Findings: (99m)Tc-labeled ASCs (1 x 10(6) cells) isolated from isogenic Lewis rats were injected 24 hours post-MI using fibrin a, collagen (ASC/C), or culture medium (ASC/M) as vehicle, and cell body distribution was assessed 24 hours later by gamma-emission counting of harvested organs. ASC/F and ASC/C groups retained significantly more cells in the myocardium than ASC/M (13.8+/-2.0 and 26.8+/-2.4% vs. 4.8+/-0.7%, respectively). Then, morphometric and direct cardiac functional parameters were evaluated 4 weeks post-MI cell injection. Left ventricle (LV) perimeter and percentage of interstitial collagen in the spare myocardium were significantly attenuated in all ASC-treated groups compared to the non-treated (NT) and control groups (culture medium, fibrin, or collagen alone). Direct hemodynamic assessment under pharmacological stress showed that stroke volume (SV) and left ventricle end-diastolic pressure were preserved in ASC-treated groups regardless of the vehicle used to deliver ASCs. Stroke work (SW), a global index of cardiac function, improved in ASC/M while it normalized when biopolymers were co-injected with ASCs. A positive correlation was observed between cardiac ASCs retention and preservation of SV and improvement in SW post-MI under hemodynamic stress. Conclusions: We provided direct evidence that intramyocardial injection of ASCs mitigates the negative cardiac remodeling and preserves ventricular function post-MI in rats and these beneficial effects can be further enhanced by administrating co-injection of ASCs with biopolymers.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[01/0009-0]Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[05/54695-3]Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[04/06784-4]Ministerio da Ciencia e Tecnologia/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/Ministerio da Saude/Departamento Ciencia e Tecnologia (MCT/CNPq/MS/DECIT)[552324/20005-1]Ministerio da Ciencia e Tecnologia/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/Ministerio da Saude/Departamento Ciencia e Tecnologia (MCT/CNPq/MS/DECIT)[10120104096700]CNPq[141276/2004-5