Achieving genuinely dynamic road user charging : issues with a GNSS-based approach

Peer reviewedPostprin

Effects of age, education and gender in the Consortium to Establish a Registry for the Alzheimer's Disease (CERAD)-Neuropsychological Assessment Battery for Cantonese-speaking Chinese elders

2010-2011 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

The genome and sex-dependent responses to temperature in the common yellow butterfly, Eurema hecabe

This is the final version. Available on open access from BMC via the DOI in this recordAvailability of data and materials: The raw reads generated in this study have been deposited to the NCBI database under the BioProject accession PRJNA664668 [110]. The final chromosome assembly was submitted to NCBI Assembly under accession number JADANM000000000 in NCBI [111]. The genome annotation files were deposited in the Figshare [112].BACKGROUND: Lepidoptera (butterflies and moths) is one of the most geographically widespread insect orders in the world, and its species play important and diverse ecological and applied roles. Climate change is one of the biggest challenges to biodiversity this century, and lepidopterans are vulnerable to climate change. Temperature-dependent gene expression differences are of relevance under the ongoing climate crisis. However, little is known about how climate affects gene expression in lepidopterans and the ecological consequences of this, particularly with respect to genes with biased expression in one of the sexes. The common yellow butterfly, Eurema hecabe (Family Pieridae), is one of the most geographically widespread lepidopterans that can be found in Asia, Africa, and Australia. Nevertheless, what temperature-dependent effects there may be and whether the effects differ between the sexes remain largely unexplored. RESULTS: Here, we generated high-quality genomic resources for E. hecabe along with transcriptomes from eight developmental stages. Male and female butterflies were subjected to varying temperatures to assess sex-specific gene expression responses through mRNA and microRNA transcriptomics. We find that there are more temperature-dependent sex-biased genes in females than males, including genes that are involved in a range of biologically important functions, highlighting potential ecological impacts of increased temperatures. Further, by considering available butterfly data on sex-biased gene expression in a comparative genomic framework, we find that the pattern of sex-biased gene expression identified in E. hecabe is highly species-specific, rather than conserved across butterfly species, suggesting that sex-biased gene expression responses to climate change are complex in butterflies. CONCLUSIONS: Our study lays the foundation for further understanding of differential responses to environmental stress in a widespread lepidopteran model and demonstrates the potential complexity of sex-specific responses of lepidopterans to climate change.Hong Kong Research Grant Council Collaborative Research Fund CRFGeneral Research Fund GRFArea of ExcellenceChinese University of Hong KongBiotechnology and Biological Sciences Research Council (BBSRC

Collaborative community based care for people and their families living with schizophrenia in India: protocol for a randomised controlled trial

BACKGROUND: There is a large treatment gap with few community services for people with schizophrenia in low income countries largely due to the shortage of specialist mental healthcare human resources. Community based rehabilitation (CBR), involving lay health workers, has been shown to be feasible, acceptable and more effective than routine care for people with schizophrenia in observational studies. The aim of this study is to evaluate whether a lay health worker led, Collaborative Community Based Care (CCBC) intervention, combined with usual Facility Based Care (FBC), is superior to FBC alone in improving outcomes for people with schizophrenia and their caregivers in India. METHODS/DESIGN: This trial is a multi-site, parallel group randomised controlled trial design in India.The trial will be conducted concurrently at three sites in India where persons with schizophrenia will be screened for eligibility and recruited after providing informed consent. Trial participants will be randomly allocated in a 2:1 ratio to the CCBC+FBC and FBC arms respectively using an allocation sequence pre-prepared through the use of permuted blocks, stratified within site. The structured CCBC intervention will be delivered by trained lay community health workers (CHWs) working together with the treating Psychiatrist. We aim to recruit 282 persons with schizophrenia. The primary outcomes are reduction in severity of symptoms of schizophrenia and disability at 12 months. The study will be conducted according to good ethical practice, data analysis and reporting guidelines. DISCUSSION: If the additional CCBC intervention delivered by front line CHWs is demonstrated to be effective and cost-effective in comparison to usually available care, this intervention can be scaled up to expand coverage and improve outcomes for persons with schizophrenia and their caregivers in low income countries. TRIAL REGISTRATION: The trial is registered with the International Society for the Registration of Clinical Trials and the allocated unique ID number is ISRCTN 56877013

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Peopling mountain environments: Changing Andean livelihoods in north-west Argentina

Structural adjustment and neoliberal policy implementation in Latin America have had dramatic consequences for livelihoods and patterns of natural resource use in mountain regions. Restructuring of the agricultural economy has increased socio-economic hardship and reduced industrial labour requirements, altering traditional patterns of seasonal migration from these areas. This paper examines the implications of recent economic and political transformation for Andean livelihoods in the mountains of northwest Argentina. Case study material illustrates the local impacts of such changes on socio-economic dynamics, patterns of urban–rural interaction, and natural resource use. The research highlights the influence of agro-industrial restructuring, protected areas creation, and the distribution of social funds in the region. It reveals that local development is constrained and controlled not only by distant policies but also by contemporary local networks of political clientalism. The influence of both distant and proximate factors governing livelihoods and environmental impacts reinforces the value of geographical study in mountain areas, given its acute spatial and scalar awareness. The paper reaffirms the conception of mountain livelihoods as diverse and dynamic, shaped by economic, political, social and cultural factors as well as physical eality, and critiques the economic rationality of resource use assumed by policymakers and economic models

The Congo: an opportunity for American leadership

Document collected by the University of Texas Libraries from the web-site of the Reseau Documentaire International Sur La Region Des Grands Lacs Africains (International Documentation Network on the Great African Lakes Region). The Reseau distributes "gray literature", non-published or limited distribution government or NGO documents regarding the Great Lakes area of central Africa including Rwanda, Burundi, and the Democratic Republic of Congo.UT Librarie

Long term outcome of patients undergoing combined transcranial and transsphenoidal resection of giant pituitary adenomas – a retrospective study

Objective: The extended endoscopic transsphenoidal approach for tackling giant pituitary adenomas, albeit safe and effective, is technically challenging and requires expertise. A widely adopted alternative is the “above and below” approach which combines the best of both transcranial and transsphenoidal approaches: it aids complete resection of large suprasellar lesions whilst reducing the risk of hemorrhage within the intradural space respectively. Here we present our experience in treating giant pituitary adenomas via a single-setting combined approach, with a focus on the long term outcomes and clinical implications on patient management. Method: A retrospective review was carried out on patients undergoing combined approach for treatment of pituitary adenoma in our center during October 2000 – October 2017. Outcomes in terms of completeness of resection, surgical complications, permanent endocrine and visual deficits, resolution of presenting symptoms and presence of residual or recurrent tumors were reviewed. Results: Our study included 25 patients, 16 women and 9 men with an average age of presentation at 49 years (range 17 – 85 years). 20 patients (80%) presented to us for the first time, whilst 5 patients (20%) had history of prior resection. Symptoms on presentation include visual disturbance (92%), obstructive hydrocephalus (8%), acute confusion (8%), acromegaly (4%), and cranial nerve palsy (4%). Tumor height range was 2.4 – 5.8cm. There were three patients (12%) with CSF leakage, three incidents of cranial nerve injury (12%), two graft donor site complications (8%), one case of meningitis (4%), and one case of deep vein thrombosis and subsequent pulmonary embolism (4%). No post-operative intracranial hemorrhage or operative mortality was reported. Permanent hormonal deficit was noted in 21 patients (84%), whilst 14 patients (56%) had persistent visual defects. On discharge, 11 patients (44%) had complete resolution of presenting symptoms. 56% gross total removal was achieved: 10 patients remained tumor-free; 3 patients had residual disease of which 1 required radiotherapy for progressive disease; and 1 patient refused post-operative MRI resulting in unknown tumor status. Subtotal removal was performed in 44% of cases with 6 patients having static residual disease. 10 patients in total received post-operative radiotherapy whilst two patients are pending reassessment imaging before deciding if adjuvant therapy is needed. Conclusion: With limited existing data, it is difficult to make direct comparisons on the safety and effectiveness of combined approaches in treating pituitary macroadenomas. However, from our results alone, we can conclude a 100% success rate in avoiding post-operative intracranial hemorrhage, which is an important advantage advocated by this technique. Furthermore, with the exception of one case, all our patients achieved either tumor-free status or static residual disease. The application of this surgical technique in clinical practice still requires further studies to analyze which group of patients would benefit the greatest from this approach