Impact of decitabine on immunohistochemistry expression of the putative tumor suppressor genes FHIT, WWOX, FUS1 and PTEN in clinical tumor samples.

BackgroundSince tumor suppressor gene function may be lost through hypermethylation, we assessed whether the demethylating agent decitabine could increase tumor suppressor gene expression clinically. For fragile histidine triad (FHIT), WW domain-containing oxidoreductase (WWOX), fused in sarcoma-1 (FUS1) and phosphatase and tensin homolog (PTEN), immunohistochemistry scores from pre- and post-decitabine tumor biopsies (25 patients) were correlated with methylation of the long interspersed nuclear element-1 (LINE-1) repetitive DNA element (as a surrogate for global DNA methylation) and with tumor regression.ResultsWith negative staining pre-decitabine (score = 0), the number of patients converting to positive staining post-decitabine was 1 of 1 for FHIT, 3 of 6 for WWOX, 2 of 3 for FUS1 and 1 of 10 for PTEN. In tumors with low pre-decitabine tumor suppressor gene scores (≤150), expression was higher post-treatment in 8 of 8 cases for FHIT (P = 0.014), 7 of 17 for WWOX (P = 0.0547), 7 of 12 for FUS1 (P = 0.0726), and 1 of 16 for PTEN (P = 0.2034). If FHIT, WWOX and FUS1 were considered together, median pre- versus post-decitabine scores were 60 versus 100 (P = 0.0002). Overall, tumor suppressor gene expression change did not correlate with LINE-1 demethylation, although tumors converting from negative to positive had a median decrease in LINE-1 methylation of 24%, compared to 6% in those not converting (P = 0.069). Five of 15 fully evaluable patients had reductions in tumor diameter (range 0.2% to 33.4%). Of these, three had simultaneous increases in three tumor suppressor genes (including the two patients with the greatest tumor regression) compared to 2 of 10 with tumor growth (P = 0.25).ConclusionsIn tumors with low tumor suppressor gene expression, decitabine may be associated with increased expression of the tumor suppressor genes FHIT, FUS1, and WWOX, but not PTEN

Variability in high-mass X-ray binaries

Strongly magnetized, accreting neutron stars show periodic and aperiodic variability over a wide range of time scales. By obtaining spectral and timing information on these different time scales, we can have a closer look into the physics of accretion close to the neutron star and the properties of the accreted material. One of the most prominent time scales is the strong pulsation, i.e., the rotation period of the neutron star itself. Over one rotation, our view of the accretion column and the X-ray producing region changes significantly. This allows us to sample different physical conditions within the column but at the same time requires that we have viewing-angle-resolved models to properly describe them. In wind-fed high-mass X-ray binaries, the main source of aperiodic variability is the clumpy stellar wind, which leads to changes in the accretion rate (i.e., luminosity) as well as absorption column. This variability allows us to study the behavior of the accretion column as a function of luminosity, as well as to investigate the structure and physical properties of the wind, which we can compare to winds in isolated stars.Comment: 6 pages, 4 figures, accepted for publication in Astronomische Nachrichten (proceedings of the XMM-Newton Workshop 2019

The Multispectral Microscopic Imager: Integrating Microimaging with Spectroscopy for the In-Situ Exploration of the Moon

To maximize the scientific return, future robotic and human missions to the Moon will need to have in-situ capabilities to enable the selection of the highest value samples for returning to Earth, or a lunar base for analysis. In order to accomplish this task efficiently, samples will need to be characterized using a suite of robotic instruments that can provide crucial information about elemental composition, mineralogy, volatiles and ices. Such spatially-correlated data sets, which place mineralogy into a microtextural context, are considered crucial for correct petrogenetic interpretations. . Combining microscopic imaging with visible= nearinfrared reflectance spectroscopy, provides a powerful in-situ approach for obtaining mineralogy within a microtextural context. The approach is non-destructive and requires minimal mechanical sample preparation. This approach provides data sets that are comparable to what geologists routinely acquire in the field, using a hand lens and in the lab using thin section petrography, and provide essential information for interpreting the primary formational processes in rocks and soils as well as the effects of secondary (diagenetic) alteration processes. Such observations lay a foundation for inferring geologic histories and provide "ground truth" for similar instruments on orbiting satellites; they support astronaut EVA activities and provide basic information about the physical properties of soils required for assessing associated health risks, and are basic tools in the exploration for in-situ resources to support human exploration of the Moon

A multi-decadal meridional displacement of the Subpolar Front in the Newfoundland Basin

Observations since the 1950s show a multi-decadal cycle of a meridional displacement of the Subpolar Front (SPF) in the Newfoundland Basin (NFB) in the North Atlantic. The SPF displacement is associated with corresponding variations in the path of the North Atlantic Current. We use the ocean general circulation model MPIOM with enhanced horizontal and vertical resolutions and forced with NCEP/NCAR reanalysis data to study the relation of the SPF displacement to atmospheric forcing, intensities of the subpolar gyre (SPG) and Meridional Overturning Circulation (MOC), and Labrador Sea Water (LSW) volume. The simulations indicate that the SPF displacement is associated with a circulation anomaly between the SPG and the subtropical gyre (STG), an inter-gyre gyre with a multi-decadal time scale. A sensitivity experiment indicates that both wind stress curl (WSC) and heat fluxes (which match LSW changes) contribute to the circulation anomalies in the frontal region and to the SPF displacement. An anticyclonic inter-gyre gyre is related to negative WSC and LSW anomalies and to a SPF north of its climatological position, indicating an expanding STG. A cyclonic inter-gyre gyre is related to positive WSC and LSW anomalies and a SPF south of its climatological position, indicating an expanding SPG. Therefore, the mean latitudinal position of the SPF in the NFB (a "SPF index") could be an indicator of the amount of LSW in the inter-gyre region. Spreading of LSW anomalies intensifies the MOC, suggesting our SPF index as predictor of the MOC intensity at multi-decadal time scales. The meridional displacement of the SPF has a pronounced influence on the meridional heat transport, both on its gyre and overturning components.<br

Deforming baryons into confining strings

We find explicit probe D3-brane solutions in the infrared of the Maldacena-Nunez background. The solutions describe deformed baryon vertices: q external quarks are separated in spacetime from the remaining N-q. As the separation is taken to infinity we recover known solutions describing infinite confining strings in ${\mathcal{N}}=1$ gauge theory. We present results for the mass of finite confining strings as a function of length. We also find probe D2-brane solutions in a confining type IIA geometry, the reduction of a G_2 holonomy M theory background. The relation between these deformed baryons and confining strings is not as straightforward.Comment: 1+13 pages. LaTeX. 3 Figures. Factor of 2N fixed to N for the IIA background. Minor changes to tex

Wavelet analysis of epileptic spikes

Interictal spikes and sharp waves in human EEG are characteristic signatures of epilepsy. These potentials originate as a result of synchronous, pathological discharge of many neurons. The reliable detection of such potentials has been the long standing problem in EEG analysis, especially after long-term monitoring became common in investigation of epileptic patients. The traditional definition of a spike is based on its amplitude, duration, sharpness, and emergence from its background. However, spike detection systems built solely around this definition are not reliable due to the presence of numerous transients and artifacts. We use wavelet transform to analyze the properties of EEG manifestations of epilepsy. We demonstrate that the behavior of wavelet transform of epileptic spikes across scales can constitute the foundation of a relatively simple yet effective detection algorithm.Comment: 4 pages, 3 figure

Predictive Power of Strong Coupling in Theories with Large Distance Modified Gravity

We consider theories that modify gravity at cosmological distances, and show that any such theory must exhibit a strong coupling phenomenon, or else it is either inconsistent or is already ruled out by the solar system observations. We show that all the ghost-free theories that modify dynamics of spin-2 graviton on asymptotically flat backgrounds, automatically have this property. Due to the strong coupling effect, modification of the gravitational force is source-dependent, and for lighter sources sets in at shorter distances. This universal feature makes modified gravity theories predictive and potentially testable not only by cosmological observations, but also by precision gravitational measurements at scales much shorter than the current cosmological horizon. We give a simple parametrization of consistent large distance modified gravity theories and their predicted deviations from the Einsteinian metric near the gravitating sources.Comment: 12 pages, Latex, to be published in New Journal of Physic

Fungi in a Warmer World - Fungal diversity from the Peak Warming of the Miocene Climate Optimum as Recorded in the Latah Formation, Clarkia, Idaho, USA

Microfungi are a vital part of ecosystems as they help with key processes, such as carbon and nutrient cycling, especially through the actions of mycorrhizal and saprotrophic members (Nuñez Otaño et al., 2015, 2021; Willis et al., 2018). Microfungi can also be good indicators of plant biodiversity in an area because many fungal taxa are host-specific (Rutten et al., 2021; Francioli et al., 2021; Hu et al., 2021; Wijayawardene et al., 2022 ). Despite being crucial components in ecosystems, they are often overlooked. In the fossil record, microfungi have a high preservaon rate and they are often preserved close to the original substrate they were deposited in. This makes them an important proxy for understanding local past ecological and climatological conditions (Romero et al., 2021, O’Keefe et al., 2017). The Fungi in a Warmer World project seeks to use fossil fungal assemblages to study changes in biodiversity during the Miocene Climate Opmum (MCO), a period of peak warming that closely mirrors current and projected warming trends (Steinthorsdotter et al., 2021). The current atmospheric CO2 concentraon is around 420 ppm but is rapidly approaching the MCO average of 450-550 ppm (Steinthorsdotter et al., 2021).https://scholarworks.moreheadstate.edu/celebration_posters_2022/1045/thumbnail.jp

Superconformal Gauge Theories and Non-Critical Superstrings

We consider effective actions for six-dimensional non-critical superstrings. We show that the addition of $N$ units of R-R flux and of $N_f$ space-time filling D5-branes produces $AdS_5 \times S^1$ solutions with curvature comparable to the string scale. These solutions have the right structure to be dual to ${\cal N}=1$ supersymmetric SU(N) gauge theories with $N_f$ flavors. We further suggest bounds on the mass-squared of tachyonic fields in this background that should restrict the theory to the conformal window.Comment: 16 pages, 1 figure v4: Minor change