Computer program developed for flowsheet calculations and process data reduction

Computer program PACER-65, is used for flowsheet calculations and easily adapted to process data reduction. Each unit, vessel, meter, and processing operation in the overall flowsheet is represented by a separate subroutine, which the program calls in the order required to complete an overall flowsheet calculation

Differential cargo mobilisation within Weibel-Palade bodies after transient fusion with the plasma membrane.

Inflammatory chemokines can be selectively released from Weibel-Palade bodies (WPBs) during kiss-and-run exocytosis. Such selectivity may arise from molecular size filtering by the fusion pore, however differential intra-WPB cargo re-mobilisation following fusion-induced structural changes within the WPB may also contribute to this process. To determine whether WPB cargo molecules are differentially re-mobilised, we applied FRAP to residual post-fusion WPB structures formed after transient exocytosis in which some or all of the fluorescent cargo was retained. Transient fusion resulted in WPB collapse from a rod to a spheroid shape accompanied by substantial swelling (>2 times by surface area) and membrane mixing between the WPB and plasma membranes. Post-fusion WPBs supported cumulative WPB exocytosis. To quantify diffusion inside rounded organelles we developed a method of FRAP analysis based on image moments. FRAP analysis showed that von Willebrand factor-EGFP (VWF-EGFP) and the VWF-propolypeptide-EGFP (Pro-EGFP) were immobile in post-fusion WPBs. Because Eotaxin-3-EGFP and ssEGFP (small soluble cargo proteins) were largely depleted from post-fusion WPBs, we studied these molecules in cells preincubated in the weak base NH4Cl which caused WPB alkalinisation and rounding similar to that produced by plasma membrane fusion. In these cells we found a dramatic increase in mobilities of Eotaxin-3-EGFP and ssEGFP that exceeded the resolution of our method (∼ 2.4 µm2/s mean). In contrast, the membrane mobilities of EGFP-CD63 and EGFP-Rab27A in post-fusion WPBs were unchanged, while P-selectin-EGFP acquired mobility. Our data suggest that selective re-mobilisation of chemokines during transient fusion contributes to selective chemokine secretion during transient WPB exocytosis. Selective secretion provides a mechanism to regulate intravascular inflammatory processes with reduced risk of thrombosis

Experimental investigation on rapid filling of a large-scale pipeline

This study presents results from detailed experiments of the two-phase pressurized flow behavior during the rapid filling of a large-scale pipeline. The physical scale of this experiment is close to the practical situation in many industrial plants. Pressure transducers, water level meters, thermometers, void fraction meters and flow meters were used to measure the two-phase unsteady flow dynamics. The main focus is on the water-air interface evolution during filling and the overall behavior of the lengthening water column. It is observed that the leading liquid front does not entirely fill the pipe cross section; flow stratification and mixing occurs. Although flow regime transition is a rather complex phenomenon, certain features of the observed transition pattern are explained qualitatively and quantitatively. The water flow during the entire filling behaves as a rigid column as the open empty pipe in front of the water column provides sufficient room for the water column to occupy without invoking air compressibility effects. As a preliminary evaluation of how these large-scale experiments can feed into improving mathematical modeling of rapid pipe filling, a comparison with a typical one-dimensional rigid-column model is made

Experimental study of filling and emptying of a large-scale pipeline

The ¿lling with liquid of an initially empty pipeline and its counterpart, the draining of an initially liquid-¿lled pipeline, are of great interest due to the many practical applications. Several potential problems may occur, of which water-hammer and slug impact are the most important. To investigate the ¿lling and emptying processes, di¿erent mathematical models have been proposed, in which a common assumption is that the water column evolves with unchanged front and/or tail. This is a reasonable assumption for small-scale systems, particularly in cases with relatively high upstream pressure head and low downstream resistance. However, it is not clear whether this assumption is applicable to large-scale systems. This issue is of high importance for the development of air pockets and gravity currents in pipelines during ¿lling and draining processes. This study presents the experimental results of the ¿ow behaviour during the rapid ¿lling and emptying of a large-scale pipeline. The experimental apparatus was designed and built at Deltares, Delft, The Netherlands, as part of the EC Hydralab III project. Di¿erent from other laboratory studies, the scale of this experiment is close to the practical situation in many industrial plants. The test rig includes a variety of components (e.g. tanks, ¿ow meters, valves, pipes of di¿erent materials) and the operation procedure is rather complex. The ¿ow behaviour is measured by various instruments and hence a thorough hydrodynamic analysis is possible. All these features make the current study particularly useful as a test case for real ¿lling and draining situations. In the ¿lling of an initially empty pipeline, the focus was on the overall behaviour of the lengthening water column and the water-air interface evolution. In the emptying of an initially water-¿lled pipeline, together with the hydrodynamics of the shortening water column, the shape and behaviour of the water tail (air-water interface) was investigated. Thirteen di¿erent combinations of initial upstream driving air pressure and downstream valve resistance were tested. The in¿uence of these two factors on the out¿ow rate is clari¿ed. It was con¿rmed that both the in¿ow front in ¿lling and the out¿ow tail in emptying do not entirely ¿ll the pipe cross section. Shape changes occur at both the water-air and air-water interfaces. Although the ¿ow regime transition is a rather complex phenomenon, certain features of the transition pattern are observed and explained qualitatively and quantitatively

Interprofessional Education: An evaluation of a joint learning workshop for podiatry and pharmacy students

"Interprofessional Education occurs when two or more professionals learn with, from and about each other to improve collaboration and the quality of care" (CAIPE 2002). Interprofessional education forms part of the Standards for the Initial Education and Training of Pharmacists. Working with and understanding the role of another profession has been shown to positively impact on the quality of care of the patient. Following positive pharmacy student feedback from visits to podiatry clinics an interprofessional learning workshop with case - based scenarios was developed. These were based on patients with high risk medical conditions that would impact on the work of both professions. Data from the feedback forms was evaluated and analysed to determine whether the workshop increased knowledge of the British National Formulary (BNF), the prescribing process and gave an insight in to the role of other healthcare professionals. We discuss how the student’s learning has been enhanced by the contribution of another professional group. The workshop was positively received. Students were observed working together discussing the patients’ conditions and issues relating to their care. This initially revolved around the students’ area of knowledge; however, as the session progressed it became apparent that the students were learning with, from and about each other for the benefit of patient care

Fast flowing populations are not well mixed

In evolutionary dynamics, well-mixed populations are almost always associated with all-to-all interactions; mathematical models are based on complete graphs. In most cases, these models do not predict fixation probabilities in groups of individuals mixed by flows. We propose an analytical description in the fast-flow limit. This approach is valid for processes with global and local selection, and accurately predicts the suppression of selection as competition becomes more local. It provides a modelling tool for biological or social systems with individuals in motion.Comment: 19 pages, 8 figure

Investigating and learning lessons from early experiences of implementing ePrescribing systems into NHS hospitals:a questionnaire study

Background: ePrescribing systems have significant potential to improve the safety and efficiency of healthcare, but they need to be carefully selected and implemented to maximise benefits. Implementations in English hospitals are in the early stages and there is a lack of standards guiding the procurement, functional specifications, and expected benefits. We sought to provide an updated overview of the current picture in relation to implementation of ePrescribing systems, explore existing strategies, and identify early lessons learned.Methods: a descriptive questionnaire-based study, which included closed and free text questions and involved both quantitative and qualitative analysis of the data generated.Results: we obtained responses from 85 of 108 NHS staff (78.7% response rate). At least 6% (n = 10) of the 168 English NHS Trusts have already implemented ePrescribing systems, 2% (n = 4) have no plans of implementing, and 34% (n = 55) are planning to implement with intended rapid implementation timelines driven by high expectations surrounding improved safety and efficiency of care. The majority are unclear as to which system to choose, but integration with existing systems and sophisticated decision support functionality are important decisive factors. Participants highlighted the need for increased guidance in relation to implementation strategy, system choice and standards, as well as the need for top-level management support to adequately resource the project. Although some early benefits were reported by hospitals that had already implemented, the hoped for benefits relating to improved efficiency and cost-savings remain elusive due to a lack of system maturity.Conclusions: whilst few have begun implementation, there is considerable interest in ePrescribing systems with ambitious timelines amongst those hospitals that are planning implementations. In order to ensure maximum chances of realising benefits, there is a need for increased guidance in relation to implementation strategy, system choice and standards, as well as increased financial resources to fund local activitie

Curved Tails in Polymerization-Based Bacterial Motility

The curved actin ``comet-tail'' of the bacterium Listeria monocytogenes is a visually striking signature of actin polymerization-based motility. Similar actin tails are associated with Shigella flexneri, spotted-fever Rickettsiae, the Vaccinia virus, and vesicles and microspheres in related in vitro systems. We show that the torque required to produce the curvature in the tail can arise from randomly placed actin filaments pushing the bacterium or particle. We find that the curvature magnitude determines the number of actively pushing filaments, independent of viscosity and of the molecular details of force generation. The variation of the curvature with time can be used to infer the dynamics of actin filaments at the bacterial surface.Comment: 8 pages, 2 figures, Latex2