II Workshop on Late Neolithic Ceramics in Ancient Mesopotamia : pottery in context

Aquest volum és el resultat del workshop celebrat per investigadors i especialistes en ceràmiques del Pròxim Orient que va tenir lloc al MAC-Empúries l'octubre de 2015. Els articles compilats en el llibre han estat escrits per 31 investigadors de 13 nacionalitats diferents i abasten temàtiques diverses al voltant de la producció ceràmica: matèries primeres, tècniques, analítiques, etc. Pel que fa al context geogràfic, els estudis se centren a Turquia, Siria, Irak, Jordània, Israel i Palestina, els països en que va aparèixer la ceràmica per primera vegada en l'àrea mediterrània, i on va experimentar un ràpid procés de transformació morfotipològic i tecnològic

A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms

Electron microscopy (EM) achieves the highest spatial resolution in protein localization, but specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ visualization in cells and tissues. Here we introduce “miniSOG” (for mini Singlet Oxygen Generator), a fluorescent flavoprotein engineered from Arabidopsis phototropin 2. MiniSOG contains 106 amino acids, less than half the size of Green Fluorescent Protein. Illumination of miniSOG generates sufficient singlet oxygen to locally catalyze the polymerization of diaminobenzidine into an osmiophilic reaction product resolvable by EM. MiniSOG fusions to many well-characterized proteins localize correctly in mammalian cells, intact nematodes, and rodents, enabling correlated fluorescence and EM from large volumes of tissue after strong aldehyde fixation, without the need for exogenous ligands, probes, or destructive permeabilizing detergents. MiniSOG permits high quality ultrastructural preservation and 3-dimensional protein localization via electron tomography or serial section block face scanning electron microscopy. EM shows that miniSOG-tagged SynCAM1 is presynaptic in cultured cortical neurons, whereas miniSOG-tagged SynCAM2 is postsynaptic in culture and in intact mice. Thus SynCAM1 and SynCAM2 could be heterophilic partners. MiniSOG may do for EM what Green Fluorescent Protein did for fluorescence microscopy

HISTÒRIA; ARQUEOLOGIA

UN NOU JACIMENT PREHISTÒRIC A LA VALL DEL TER: CAN FELÓ (TAVERTET